Protein : Qrob_P0173090.2 Q. robur
Protein Identifier  ? Qrob_P0173090.2 Organism . Name  Quercus robur
Score  98.0 Score Type  egn
Protein Description  (M=1) 2.7.1.49 - Hydroxymethylpyrimidine kinase. Code Enzyme  EC:2.5.1.3, EC:2.7.1.49
Gene Prediction Quality  validated Protein length 

Sequence

Length: 564  
Kegg Orthology  K14153
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0009228 thiamine biosynthetic process The chemical reactions and pathways resulting in the formation of thiamine (vitamin B1), a water soluble vitamin present in fresh vegetables and meats, especially liver.
GO:0004789 thiamine-phosphate diphosphorylase activity Catalysis of the reaction: 4-amino-2-methyl-5-diphosphomethylpyrimidine + 4-methyl-5-(2-phosphoethyl)-thiazole + H(+) = diphosphate + thiamine phosphate.
GO:0008972 phosphomethylpyrimidine kinase activity Catalysis of the reaction: ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.

42 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|vvi:100249214 19 561 + 543 Gaps:1 79.59 681 82.84 0.0 putative hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 2-like
blastp_kegg lcl|pmum:103318894 17 556 + 540 Gaps:1 95.40 565 80.89 0.0 thiamine biosynthetic bifunctional enzyme TH1 chloroplastic
blastp_kegg lcl|pper:PRUPE_ppa003609mg 1 558 + 558 Gaps:3 99.47 562 79.25 0.0 hypothetical protein
blastp_kegg lcl|pxb:103940368 4 556 + 553 Gaps:8 98.21 559 80.69 0.0 thiamine biosynthetic bifunctional enzyme TH1 chloroplastic
blastp_kegg lcl|tcc:TCM_006934 12 556 + 545 Gaps:9 95.12 574 78.94 0.0 Thiamin biosynthesis protein isoform 1
blastp_kegg lcl|cit:102610242 30 557 + 528 Gaps:2 97.79 542 80.57 0.0 thiamine biosynthetic bifunctional enzyme TH1 chloroplastic-like
blastp_kegg lcl|cic:CICLE_v10000782mg 50 557 + 508 Gaps:2 99.22 514 81.96 0.0 hypothetical protein
blastp_kegg lcl|fve:101301972 64 556 + 493 none 98.60 500 81.74 0.0 thiamine biosynthetic bifunctional enzyme TH1 chloroplastic-like
blastp_kegg lcl|rcu:RCOM_0321500 33 558 + 526 Gaps:11 94.32 546 80.19 0.0 phosphomethylpyrimidine kinase putative (EC:2.7.4.7 2.5.1.3)
blastp_kegg lcl|cam:101509757 62 556 + 495 none 87.30 567 78.18 0.0 thiamine biosynthetic bifunctional enzyme TH1 chloroplastic-like
blastp_pdb 1jxi_B 63 328 + 266 Gaps:2 91.67 288 40.91 3e-54 mol:protein length:288 PHOSPHOMETHYLPYRIMIDINE KINASE
blastp_pdb 1jxi_A 63 328 + 266 Gaps:2 91.67 288 40.91 3e-54 mol:protein length:288 PHOSPHOMETHYLPYRIMIDINE KINASE
blastp_pdb 1jxh_B 63 328 + 266 Gaps:2 91.67 288 40.91 3e-54 mol:protein length:288 PHOSPHOMETHYLPYRIMIDINE KINASE
blastp_pdb 1jxh_A 63 328 + 266 Gaps:2 91.67 288 40.91 3e-54 mol:protein length:288 PHOSPHOMETHYLPYRIMIDINE KINASE
blastp_pdb 2i5b_E 65 331 + 267 Gaps:6 98.52 271 39.70 8e-52 mol:protein length:271 Phosphomethylpyrimidine kinase
blastp_pdb 2i5b_B 65 331 + 267 Gaps:6 98.52 271 39.70 8e-52 mol:protein length:271 Phosphomethylpyrimidine kinase
blastp_pdb 2i5b_D 65 331 + 267 Gaps:6 98.52 271 39.70 8e-52 mol:protein length:271 Phosphomethylpyrimidine kinase
blastp_pdb 2i5b_C 65 331 + 267 Gaps:6 98.52 271 39.70 8e-52 mol:protein length:271 Phosphomethylpyrimidine kinase
blastp_pdb 2i5b_A 65 331 + 267 Gaps:6 98.52 271 39.70 8e-52 mol:protein length:271 Phosphomethylpyrimidine kinase
blastp_pdb 1xi3_B 347 557 + 211 Gaps:9 93.95 215 43.07 9e-47 mol:protein length:215 Thiamine phosphate pyrophosphorylase
blastp_uniprot_sprot sp|Q5M731|TPS1L_ARATH 50 556 + 507 Gaps:2 96.74 522 76.44 0.0 Thiamine biosynthetic bifunctional enzyme TH1 chloroplastic OS Arabidopsis thaliana GN TH1 PE 1 SV 1
blastp_uniprot_sprot sp|O48881|TPS1_BRANA 66 556 + 491 Gaps:2 93.50 523 75.66 0.0 Thiamine biosynthetic bifunctional enzyme BTH1 chloroplastic OS Brassica napus GN BTH1 PE 1 SV 1
blastp_uniprot_sprot sp|Q2QWK9|TPS1_ORYSJ 63 556 + 494 none 90.15 548 71.05 0.0 Probable thiamine biosynthetic bifunctional enzyme chloroplastic OS Oryza sativa subsp. japonica GN Os12g0192500 PE 2 SV 2
blastp_uniprot_sprot sp|P56904|THID_RHIME 71 330 + 260 Gaps:3 96.62 266 48.25 4e-56 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase OS Rhizobium meliloti (strain 1021) GN thiD PE 3 SV 2
blastp_uniprot_sprot sp|P61422|THIED_GEOSL 70 537 + 468 Gaps:14 88.37 490 45.73 7e-56 Thiamine biosynthesis bifunctional protein ThiED OS Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) GN thiDE PE 3 SV 1
blastp_uniprot_sprot sp|P76422|THID_ECOLI 65 328 + 264 Gaps:2 98.50 266 41.60 5e-55 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase OS Escherichia coli (strain K12) GN thiD PE 1 SV 1
blastp_uniprot_sprot sp|O94266|THI22_SCHPO 63 368 + 306 Gaps:26 56.62 551 38.78 2e-54 Putative hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 2 OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN SPBP8B7.18c PE 3 SV 1
blastp_uniprot_sprot sp|P55882|THID_SALTY 65 328 + 264 Gaps:2 98.50 266 41.60 5e-54 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) GN thiD PE 1 SV 2
blastp_uniprot_sprot sp|A8MK92|THIE_ALKOO 348 551 + 204 Gaps:5 95.22 209 47.74 2e-51 Thiamine-phosphate synthase OS Alkaliphilus oremlandii (strain OhILAs) GN thiE PE 3 SV 1
blastp_uniprot_sprot sp|P39610|PDXK_BACSU 65 331 + 267 Gaps:6 98.52 271 39.70 3e-51 Pyridoxine kinase OS Bacillus subtilis (strain 168) GN pdxK PE 1 SV 1

11 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 55 365 311 PTHR20858:SF17 none none none
SUPERFAMILY 65 325 261 SSF53613 none none IPR029056
Hamap 349 554 206 MF_00097 "KEGG:00730+2.5.1.3","MetaCyc:PWY-6893","MetaCyc:PWY-6894","MetaCyc:PWY-6897","MetaCyc:PWY-6907","MetaCyc:PWY-6908","MetaCyc:PWY-7353","MetaCyc:PWY-7357","UniPathway:UPA00060" Thiamine-phosphate synthase [thiE]. IPR003733
Pfam 351 536 186 PF02581 "KEGG:00730+2.5.1.3","MetaCyc:PWY-6893","MetaCyc:PWY-6894","MetaCyc:PWY-6897","MetaCyc:PWY-6907","MetaCyc:PWY-6908","MetaCyc:PWY-7353","MetaCyc:PWY-7357","UniPathway:UPA00060" Thiamine monophosphate synthase/TENI IPR003733
TIGRFAM 70 327 258 TIGR00097 "KEGG:00730+2.7.1.49","MetaCyc:PWY-6910","MetaCyc:PWY-7356","MetaCyc:PWY-7357" HMP-P_kinase: hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase IPR004399
Gene3D 345 554 210 G3DSA:3.20.20.70 none none IPR013785
TIGRFAM 351 548 198 TIGR00693 "KEGG:00730+2.5.1.3","MetaCyc:PWY-6893","MetaCyc:PWY-6894","MetaCyc:PWY-6897","MetaCyc:PWY-6907","MetaCyc:PWY-6908","MetaCyc:PWY-7353","MetaCyc:PWY-7357","UniPathway:UPA00060" thiE: thiamine-phosphate diphosphorylase IPR003733
Gene3D 67 326 260 G3DSA:3.40.1190.20 none none IPR029056
SUPERFAMILY 346 552 207 SSF51391 "KEGG:00730+2.5.1.3","MetaCyc:PWY-6893","MetaCyc:PWY-6894","MetaCyc:PWY-6897","MetaCyc:PWY-6907","MetaCyc:PWY-6908","MetaCyc:PWY-7353","MetaCyc:PWY-7357","UniPathway:UPA00060" none IPR022998
PANTHER 55 365 311 PTHR20858 none none none
Pfam 77 321 245 PF08543 none Phosphomethylpyrimidine kinase IPR013749

0 Localization

0 Qtllist

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 95   Mitochondrion 5 0.027 0.481 NON-PLANT 95