Protein : Qrob_P0170630.2 Q. robur
Protein Identifier  ? Qrob_P0170630.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=6) K01176 - alpha-amylase [EC:3.2.1.1] Code Enzyme  EC:3.2.1.1
Gene Prediction Quality  validated Protein length 

Sequence

Length: 434  
Kegg Orthology  K01176
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0 Synonyms

5 GO Terms

Identifier Name Description
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0005975 carbohydrate metabolic process The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. Includes the formation of carbohydrate derivatives by the addition of a carbohydrate residue to another molecule.
GO:0005509 calcium ion binding Interacting selectively and non-covalently with calcium ions (Ca2+).
GO:0043169 cation binding Interacting selectively and non-covalently with cations, charged atoms or groups of atoms with a net positive charge.
GO:0004556 alpha-amylase activity Catalysis of the endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more alpha-(1->4)-linked D-glucose units.

42 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|vvi:100245165 1 426 + 426 Gaps:2 100.00 424 80.19 0.0 alpha-amylase-like
blastp_kegg lcl|pper:PRUPE_ppa006152mg 1 427 + 427 Gaps:2 100.00 425 77.41 0.0 hypothetical protein
blastp_kegg lcl|mdm:103436492 6 427 + 422 Gaps:3 97.45 432 76.48 0.0 alpha-amylase-like
blastp_kegg lcl|pmum:103335273 6 427 + 422 Gaps:9 98.57 419 79.42 0.0 alpha-amylase-like
blastp_kegg lcl|pmum:103335272 6 427 + 422 Gaps:5 98.59 425 79.24 0.0 alpha-amylase-like
blastp_kegg lcl|pxb:103941038 6 427 + 422 Gaps:3 97.45 432 76.72 0.0 alpha-amylase-like
blastp_kegg lcl|fve:101294444 4 425 + 422 Gaps:4 90.67 461 78.23 0.0 alpha-amylase-like
blastp_kegg lcl|vvi:100250317 1 426 + 426 Gaps:3 100.00 425 75.53 0.0 alpha-amylase-like
blastp_kegg lcl|pxb:103941903 5 427 + 423 Gaps:4 98.82 424 73.51 0.0 alpha-amylase-like
blastp_kegg lcl|mdm:103436491 5 427 + 423 Gaps:4 98.82 424 73.51 0.0 alpha-amylase-like
blastp_pdb 1bg9_A 24 425 + 402 Gaps:4 99.75 403 64.18 0.0 mol:protein length:403 1 4-ALPHA-D-GLUCAN GLUCANOHYDROLASE
blastp_pdb 1ava_B 24 425 + 402 Gaps:4 99.75 403 64.18 0.0 mol:protein length:403 BARLEY ALPHA-AMYLASE 2(CV MENUET)
blastp_pdb 1ava_A 24 425 + 402 Gaps:4 99.75 403 64.18 0.0 mol:protein length:403 BARLEY ALPHA-AMYLASE 2(CV MENUET)
blastp_pdb 1amy_A 24 425 + 402 Gaps:4 99.75 403 64.18 0.0 mol:protein length:403 1 4-ALPHA-D-GLUCAN GLUCANOHYDROLASE
blastp_pdb 2qpu_C 24 425 + 402 Gaps:5 99.51 405 61.79 0.0 mol:protein length:405 Alpha-amylase type A isozyme
blastp_pdb 2qpu_B 24 425 + 402 Gaps:5 99.51 405 61.79 0.0 mol:protein length:405 Alpha-amylase type A isozyme
blastp_pdb 2qpu_A 24 425 + 402 Gaps:5 99.51 405 61.79 0.0 mol:protein length:405 Alpha-amylase type A isozyme
blastp_pdb 3bsg_A 24 425 + 402 Gaps:5 97.34 414 61.54 0.0 mol:protein length:414 Alpha-amylase type A isozyme
blastp_pdb 1rpk_A 24 425 + 402 Gaps:5 99.51 405 61.54 0.0 mol:protein length:405 Alpha-amylase type 1 isozyme
blastp_pdb 1p6w_A 24 425 + 402 Gaps:5 99.51 405 61.54 0.0 mol:protein length:405 PROTEIN (Alpha-amylase type A isozyme)
blastp_uniprot_sprot sp|P17859|AMYA_VIGMU 7 424 + 418 Gaps:3 98.57 421 69.88 0.0 Alpha-amylase OS Vigna mungo GN AMY1.1 PE 2 SV 1
blastp_uniprot_sprot sp|P27933|AMY3D_ORYSJ 19 425 + 407 Gaps:2 92.89 436 64.44 0.0 Alpha-amylase isozyme 3D OS Oryza sativa subsp. japonica GN AMY1.3 PE 2 SV 2
blastp_uniprot_sprot sp|P27934|AMY3E_ORYSJ 10 426 + 417 Gaps:2 94.97 437 62.89 0.0 Alpha-amylase isozyme 3E OS Oryza sativa subsp. japonica GN AMY1.4 PE 2 SV 1
blastp_uniprot_sprot sp|P27939|AMY3C_ORYSJ 19 425 + 407 Gaps:2 92.68 437 64.69 0.0 Alpha-amylase isozyme 3C OS Oryza sativa subsp. japonica GN AMY1.7 PE 2 SV 2
blastp_uniprot_sprot sp|P27937|AMY3B_ORYSJ 19 425 + 407 Gaps:2 92.47 438 64.44 0.0 Alpha-amylase isozyme 3B OS Oryza sativa subsp. japonica GN AMY1.6 PE 2 SV 1
blastp_uniprot_sprot sp|P27932|AMY3A_ORYSJ 1 425 + 425 Gaps:7 97.27 440 60.05 0.0 Alpha-amylase isozyme 3A OS Oryza sativa subsp. japonica GN AMY1.2 PE 2 SV 2
blastp_uniprot_sprot sp|P04063|AMY2_HORVU 19 425 + 407 Gaps:4 95.32 427 63.39 0.0 Alpha-amylase type B isozyme OS Hordeum vulgare GN AMY1.2 PE 1 SV 3
blastp_uniprot_sprot sp|P17654|AMY1_ORYSJ 16 425 + 410 Gaps:6 94.47 434 63.41 0.0 Alpha-amylase OS Oryza sativa subsp. japonica GN AMY1.1 PE 2 SV 2
blastp_uniprot_sprot sp|Q8VZ56|AMY1_ARATH 7 426 + 420 Gaps:8 98.35 423 64.66 0.0 Alpha-amylase 1 OS Arabidopsis thaliana GN AMY1 PE 2 SV 1
blastp_uniprot_sprot sp|A2YGY2|AMY2A_ORYSI 21 426 + 406 Gaps:7 91.70 446 62.59 0.0 Alpha-amylase isozyme 2A OS Oryza sativa subsp. indica GN AMYC2 PE 2 SV 1

17 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Pfam 43 234 192 PF00128 none Alpha amylase, catalytic domain IPR006047
SUPERFAMILY 24 360 337 SSF51445 none none IPR017853
SMART 24 370 347 SM00642 none Alpha-amylase domain IPR006589
Gene3D 369 426 58 G3DSA:2.60.40.1180 none none IPR013780
Phobius 28 433 406 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Gene3D 25 368 344 G3DSA:3.20.20.80 none none IPR013781
Pfam 366 425 60 PF07821 "KEGG:00500+3.2.1.1" Alpha-amylase C-terminal beta-sheet domain IPR012850
SMART 365 425 61 SM00810 "KEGG:00500+3.2.1.1" Alpha-amylase C-terminal beta-sheet domain IPR012850
PIRSF 1 426 426 PIRSF001028 "KEGG:00500+3.2.1.1" none IPR013775
Phobius 6 27 22 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
PANTHER 5 424 420 PTHR10357 none none IPR015902
PRINTS 302 314 13 PR00110 "KEGG:00500+3.2.1.1","Reactome:REACT_474" Alpha-amylase signature IPR006046
PRINTS 106 117 12 PR00110 "KEGG:00500+3.2.1.1","Reactome:REACT_474" Alpha-amylase signature IPR006046
PRINTS 198 209 12 PR00110 "KEGG:00500+3.2.1.1","Reactome:REACT_474" Alpha-amylase signature IPR006046
Phobius 1 5 5 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
PANTHER 5 424 420 PTHR10357:SF112 none none none
SUPERFAMILY 369 425 57 SSF51011 none none none

2 Localization

Analysis Start End Length
SignalP_EUK 1 22 21
TMHMM 5 27 22

6 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2015_nEpis_3P Qrob_Chr12 12 s_1AOES6_1466 s_1B0DDG_1094 28,97 28,55 30,1 lod 3.6 8.4
Champenoux_2015_nEpis_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,53 25,47 27,72 lod 4.4 8.9
Bourran2_2014_nP_A4 Qrob_Chr11 11 s_1B58GB_1413 s_1A5BYY_1671 11,15 0 42,38 lod 1,8913 4,5
Bourran2_2002_QTL16_peak_Bud_burst_A4 Qrob_Chr11 11 s_A9OIA_166 s_1C6WBF_114 21,23 2,83 42,83 lod 5,1 7,2
Bourran2_2015_nSeqBC_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,06 25,47 27,72 lod 3.6 7.1
Champenoux_2015_nSecLBD_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 25,78 25,47 27,72 lod 6.3 16.6

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 23   Secretory pathway 1 0.978 0.014 NON-PLANT 23