Protein : Qrob_P0102030.2 Q. robur

Protein Identifier  ? Qrob_P0102030.2 Organism . Name  Quercus robur
Score  94.1 Score Type  egn
Protein Description  (M=2) K12666 - oligosaccharyltransferase complex subunit alpha (ribophorin I) Code Enzyme  EC:2.4.99.18
Gene Prediction Quality  validated Protein length 

Sequence

Length: 451  
Kegg Orthology  K12666

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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0016021 integral component of membrane The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
GO:0005783 endoplasmic reticulum The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisternae. The ER takes two forms, rough (or granular), with ribosomes adhering to the outer surface, and smooth (with no ribosomes attached).
GO:0006486 protein glycosylation A protein modification process that results in the addition of a carbohydrate or carbohydrate derivative unit to a protein amino acid, e.g. the addition of glycan chains to proteins.
GO:0004579 dolichyl-diphosphooligosaccharide-protein glycotransferase activity Catalysis of the reaction: dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by glycosylamine linkage to protein L-asparagine.

22 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pop:POPTR_0008s03860g 1 436 + 436 Gaps:7 91.47 469 79.49 0.0 POPTRDRAFT_720702 ribophorin 1 family protein
blastp_kegg lcl|pop:POPTR_0010s23030g 1 436 + 436 Gaps:7 91.47 469 78.79 0.0 POPTRDRAFT_770546 ribophorin 1 family protein
blastp_kegg lcl|pxb:103936646 1 433 + 433 Gaps:4 91.70 470 81.44 0.0 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B
blastp_kegg lcl|mdm:103406266 1 433 + 433 Gaps:4 91.70 470 80.97 0.0 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B-like
blastp_kegg lcl|rcu:RCOM_0092950 32 433 + 402 none 89.73 448 83.83 0.0 ribophorin putative (EC:2.4.1.119)
blastp_kegg lcl|pmum:103332361 1 433 + 433 Gaps:4 91.51 471 80.51 0.0 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B
blastp_kegg lcl|fve:101295172 32 433 + 402 Gaps:2 85.84 466 81.25 0.0 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B-like
blastp_kegg lcl|mdm:103430694 1 433 + 433 Gaps:8 91.77 474 80.23 0.0 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B-like
blastp_kegg lcl|pper:PRUPE_ppa005245mg 1 433 + 433 Gaps:4 91.51 471 80.05 0.0 hypothetical protein
blastp_kegg lcl|gmx:100814638 26 433 + 408 none 86.99 469 77.94 0.0 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B-like
blastp_uniprot_sprot sp|Q9ZUA0|OST1B_ARATH 1 436 + 436 Gaps:7 92.46 464 71.33 0.0 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B OS Arabidopsis thaliana GN OST1B PE 2 SV 1
blastp_uniprot_sprot sp|B9FDT1|OST1B_ORYSJ 22 433 + 412 Gaps:2 87.53 473 68.84 0.0 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B OS Oryza sativa subsp. japonica GN OST1B PE 2 SV 1
blastp_uniprot_sprot sp|Q0DJC5|OST1A_ORYSJ 26 433 + 408 Gaps:6 66.02 615 57.88 2e-159 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A OS Oryza sativa subsp. japonica GN OST1A PE 2 SV 1
blastp_uniprot_sprot sp|Q9SFX3|OST1A_ARATH 33 433 + 401 Gaps:2 65.31 614 52.62 8e-144 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A OS Arabidopsis thaliana GN OST1A PE 2 SV 1
blastp_uniprot_sprot sp|Q9GMB0|RPN1_PIG 38 425 + 388 Gaps:20 63.49 608 37.56 2e-75 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 OS Sus scrofa GN RPN1 PE 1 SV 1
blastp_uniprot_sprot sp|Q5RFB6|RPN1_PONAB 38 425 + 388 Gaps:18 63.59 607 37.31 5e-75 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 OS Pongo abelii GN RPN1 PE 2 SV 1
blastp_uniprot_sprot sp|Q4R4T0|RPN1_MACFA 38 425 + 388 Gaps:18 63.59 607 37.56 9e-75 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 OS Macaca fascicularis GN RPN1 PE 2 SV 1
blastp_uniprot_sprot sp|P04843|RPN1_HUMAN 38 425 + 388 Gaps:18 63.59 607 37.31 1e-74 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 OS Homo sapiens GN RPN1 PE 1 SV 1
blastp_uniprot_sprot sp|Q91YQ5|RPN1_MOUSE 38 425 + 388 Gaps:18 63.49 608 37.56 2e-74 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 OS Mus musculus GN Rpn1 PE 2 SV 1
blastp_uniprot_sprot sp|P07153|RPN1_RAT 38 425 + 388 Gaps:18 63.80 605 37.05 1e-72 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 OS Rattus norvegicus GN Rpn1 PE 2 SV 1
rpsblast_cdd gnl|CDD|191038 33 431 + 399 Gaps:12 92.54 429 46.10 1e-130 pfam04597 Ribophorin_I Ribophorin I. Ribophorin I is an essential subunit of oligosaccharyltransferase (OST) which is also known as Dolichyl-diphosphooligosaccharide--protein glycosyltransferase (EC:2.4.1.119). OST catalyzes the transfer of an oligosaccharide from dolichol pyrophosphate to selected asparagine residues of nascent polypeptides as they are translocated into the lumen of the rough endoplasmic reticulum. Ribophorin I and OST48 are though to be responsible for OST catalytic activity. Both yeast and mammalian proteins are glycosylated but the sites are not conserved. Glycosylation may contribute towards general solubility but is unlikely to be involved in a specific biochemical function Most family members are predicted to have a transmembrane helix at the C terminus of this region.
rpsblast_kog gnl|CDD|37502 7 433 + 427 Gaps:9 70.43 602 51.89 1e-137 KOG2291 KOG2291 KOG2291 Oligosaccharyltransferase alpha subunit (ribophorin I) [Posttranslational modification protein turnover chaperones].

8 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Phobius 28 450 423 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
PANTHER 5 431 427 PTHR21049 "KEGG:00510+2.4.99.18","KEGG:00513+2.4.99.18","UniPathway:UPA00378";signature_desc=RIBOPHORIN I none IPR007676
Phobius 9 22 14 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
PANTHER 5 431 427 PTHR21049:SF0 none none none
Pfam 34 433 400 PF04597 "KEGG:00510+2.4.99.18","KEGG:00513+2.4.99.18","UniPathway:UPA00378" Ribophorin I IPR007676
Phobius 23 27 5 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
Phobius 1 8 8 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
Phobius 1 27 27 SIGNAL_PEPTIDE none Signal peptide region none

1 Localization

Analysis Start End Length
SignalP_EUK 1 27 26

8 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nLBD*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 34,91 16,12 53,62 lod 2,4961 5,2
Bourran2_2014_rEpiBC*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 35,77 14,11 55,31 lod 2,9413 6,2
Bourran2_2015_nEpiBC_3P Qrob_Chr12 12 s_1B73S5_217 v_7050_211 28,31 26,37 28,45 lod 4.5 11.6
Bourran2_2014_aSeqBC_A4 Qrob_Chr08 8 v_15999_278 v_AP13YL15_395 32,52 4,22 57,22 lod 2,7561 6,7
Bourran2_2014_nFork*_A4 Qrob_Chr08 8 PIE175 s_1CD7GJ_1398 31,22 5,24 57,24 lod 2,6724 6,8
Bourran2_2015_rEpiBC_3P Qrob_Chr08 8 s_A9TNV_543 v_11837_70 9,93 9,83 11,15 lod 3.3 7.3
Champenoux_2015_nSeqBC_A4 Qrob_Chr08 8 v_AD7YD13_501 s_1A7IED_780 43,44 43,42 43,99 lod 3.7 8.9
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL3_d13Cf Qrob_Chr08 8 v_5216_549 v_11625_20 37.08 12,26 54,9 lod 6.5888 0.04

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 27   Secretory pathway 1 0.952 0.059 NON-PLANT 27