Protein : Qrob_P0024220.2 Q. robur
Protein Identifier  ? Qrob_P0024220.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=5) K04506 - E3 ubiquitin-protein ligase SIAH1 [EC:6.3.2.19] Code Enzyme  EC:6.3.2.19
Gene Prediction Quality  validated Protein length 

Sequence

Length: 306  
Kegg Orthology  K04506
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0 Synonyms

7 GO Terms

Identifier Name Description
GO:0005515 protein binding Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO:0008270 zinc ion binding Interacting selectively and non-covalently with zinc (Zn) ions.
GO:0004842 ubiquitin-protein transferase activity Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages.
GO:0016567 protein ubiquitination The process in which one or more ubiquitin groups are added to a protein.
GO:0007275 multicellular organismal development The biological process whose specific outcome is the progression of a multicellular organism over time from an initial condition (e.g. a zygote or a young adult) to a later condition (e.g. a multicellular animal or an aged adult).
GO:0005634 nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
GO:0006511 ubiquitin-dependent protein catabolic process The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of a ubiquitin group, or multiple ubiquitin groups, to the protein.

30 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|vvi:100244678 1 305 + 305 none 100.00 305 95.08 0.0 E3 ubiquitin-protein ligase SINAT5-like
blastp_kegg lcl|rcu:RCOM_1452850 1 305 + 305 none 100.00 305 94.75 0.0 Ubiquitin ligase SINAT5 putative
blastp_kegg lcl|tcc:TCM_045318 1 305 + 305 Gaps:1 100.00 304 94.08 0.0 RING/U-box and TRAF-like domains
blastp_kegg lcl|pop:POPTR_0003s19820g 1 305 + 305 none 100.00 305 93.11 0.0 POPTRDRAFT_711983 SINA-like family protein
blastp_kegg lcl|pper:PRUPE_ppa009142mg 1 305 + 305 none 100.00 305 92.46 0.0 hypothetical protein
blastp_kegg lcl|pmum:103343720 1 305 + 305 none 100.00 305 92.46 0.0 E3 ubiquitin-protein ligase SINAT5-like
blastp_kegg lcl|cit:102624862 1 305 + 305 Gaps:2 100.00 307 91.21 0.0 E3 ubiquitin-protein ligase SINAT5-like
blastp_kegg lcl|cic:CICLE_v10005495mg 1 305 + 305 Gaps:2 100.00 307 90.88 0.0 hypothetical protein
blastp_kegg lcl|pxb:103942729 1 305 + 305 none 100.00 305 92.13 0.0 E3 ubiquitin-protein ligase SINAT5-like
blastp_kegg lcl|pxb:103952155 1 305 + 305 none 100.00 305 92.13 0.0 E3 ubiquitin-protein ligase SINAT5-like
blastp_pdb 2a25_A 107 298 + 192 Gaps:9 97.93 193 31.75 2e-22 mol:protein length:193 Ubiquitin ligase SIAH1
blastp_pdb 2an6_D 110 298 + 189 Gaps:9 97.38 191 31.18 9e-22 mol:protein length:191 Ubiquitin ligase SIAH1A
blastp_pdb 2an6_C 110 298 + 189 Gaps:9 97.38 191 31.18 9e-22 mol:protein length:191 Ubiquitin ligase SIAH1A
blastp_pdb 2an6_B 110 298 + 189 Gaps:9 97.38 191 31.18 9e-22 mol:protein length:191 Ubiquitin ligase SIAH1A
blastp_pdb 2an6_A 110 298 + 189 Gaps:9 97.38 191 31.18 9e-22 mol:protein length:191 Ubiquitin ligase SIAH1A
blastp_pdb 1k2f_B 110 298 + 189 Gaps:9 97.89 190 31.18 1e-21 mol:protein length:190 siah-1A protein
blastp_pdb 1k2f_A 110 298 + 189 Gaps:9 97.89 190 31.18 1e-21 mol:protein length:190 siah-1A protein
blastp_uniprot_sprot sp|Q8S3N1|SINA5_ARATH 45 305 + 261 none 84.47 309 83.52 1e-169 E3 ubiquitin-protein ligase SINAT5 OS Arabidopsis thaliana GN SINAT5 PE 1 SV 2
blastp_uniprot_sprot sp|Q84JL3|SINA3_ARATH 45 305 + 261 none 80.06 326 83.52 6e-167 E3 ubiquitin-protein ligase SINAT3 OS Arabidopsis thaliana GN SINAT3 PE 2 SV 1
blastp_uniprot_sprot sp|Q9STN8|SINA4_ARATH 48 305 + 258 none 78.90 327 82.56 5e-164 E3 ubiquitin-protein ligase SINAT4 OS Arabidopsis thaliana GN SINAT4 PE 2 SV 1
blastp_uniprot_sprot sp|Q9M2P4|SINA2_ARATH 48 305 + 258 none 83.77 308 80.62 2e-159 E3 ubiquitin-protein ligase SINAT2 OS Arabidopsis thaliana GN SINAT2 PE 2 SV 1
blastp_uniprot_sprot sp|P93748|SINA1_ARATH 48 305 + 258 none 84.59 305 79.46 3e-157 Putative E3 ubiquitin-protein ligase SINAT1 OS Arabidopsis thaliana GN SINAT1 PE 3 SV 1
blastp_uniprot_sprot sp|Q86MW9|SINA_SCHMA 41 298 + 258 Gaps:9 68.73 371 35.69 9e-47 E3 ubiquitin-protein ligase sina OS Schistosoma mansoni GN SINA PE 1 SV 1
blastp_uniprot_sprot sp|Q7ZVG6|SIAH1_DANRE 40 298 + 259 Gaps:10 90.43 282 36.08 4e-45 E3 ubiquitin-protein ligase Siah1 OS Danio rerio GN siah1 PE 2 SV 2
blastp_uniprot_sprot sp|Q920M9|SIAH1_RAT 43 298 + 256 Gaps:14 90.78 282 36.33 1e-44 E3 ubiquitin-protein ligase SIAH1 OS Rattus norvegicus GN Siah1 PE 1 SV 2
blastp_uniprot_sprot sp|P61092|SIA1A_MOUSE 43 298 + 256 Gaps:14 90.78 282 36.33 1e-44 E3 ubiquitin-protein ligase SIAH1A OS Mus musculus GN Siah1a PE 1 SV 1
blastp_uniprot_sprot sp|Q8IUQ4|SIAH1_HUMAN 43 298 + 256 Gaps:14 90.78 282 36.33 2e-44 E3 ubiquitin-protein ligase SIAH1 OS Homo sapiens GN SIAH1 PE 1 SV 2
rpsblast_cdd gnl|CDD|190542 99 298 + 200 Gaps:2 100.00 198 43.43 7e-68 pfam03145 Sina Seven in absentia protein family. The seven in absentia (sina) gene was first identified in Drosophila. The Drosophila Sina protein is essential for the determination of the R7 pathway in photoreceptor cell development: the loss of functional Sina results in the transformation of the R7 precursor cell to a non- neuronal cell type. The Sina protein contains an N-terminal RING finger domain pfam00097. Through this domain Sina binds E2 ubiquitin-conjugating enzymes (UbcD1) Sina also interacts with Tramtrack (TTK88) via PHYL. Tramtrack is a transcriptional repressor that blocks photoreceptor determination while PHYL down-regulates the activity of TTK88. In turn the activity of PHYL requires the activation of the Sevenless receptor tyrosine kinase a process essential for R7 determination. It is thought that thus Sina targets TTK88 for degradation therefore promoting the R7 pathway. Murine and human homologues of Sina have also been identified. The human homologue Siah-1 also binds E2 enzymes (UbcH5) and through a series of physical interactions targets beta-catenin for ubiquitin degradation. Siah-1 expression is enhanced by p53 itself promoted by DNA damage. Thus this pathway links DNA damage to beta-catenin degradation. Sina proteins therefore physically interact with a variety of proteins. The N-terminal RING finger domain that binds ubiquitin conjugating enzymes is described in pfam00097 and does not form part of the alignment for this family. The remainder C-terminal part is involved in interactions with other proteins and is included in this alignment. In addition to the Drosophila protein and mammalian homologues whose similarity was noted previously this family also includes putative homologues from Caenorhabditis elegans Arabidopsis thaliana.
rpsblast_cdd gnl|CDD|63889 171 299 + 129 Gaps:4 100.00 127 29.92 5e-47 cd03829 Sina Seven in absentia (Sina) protein family C-terminal substrate binding domain composed of the Drosophila Sina protein the mammalian Sina homolog (Siah) the plant protein SINAT5 and similar proteins. Sina Siah and SINAT5 are RING-containing proteins that function as E3 ubiquitin ligases acting either as single proteins or as a part of multiprotein complexes. Sina is expressed in many cells in the developing eye but is essential specifically for R7 photoreceptor cell development. Sina cooperates with Phyllopod (Phyl) Ebi and the E2 ubiquitin-conjugating enzyme Ubcd1 to catalyze the ubiquitination and subsequent degradation of Tramtrack (Ttk88) Ttk88 is a transcriptional repressor that blocks photoreceptor differentiation. Similarly the mammalian homologue Siah1 cooperates with SIP (Siah-interacting protein) Ebi and the adaptor protein Skp1 to target beta-catenin for ubiquitination and degradation via a p53-dependent mechanism. SINAT5 targets NAC1 for ubiquitin-mediated degradation resulting in the downregulation of auxin a hormone that controls many aspects of plant development. Other targets of Sina family proteins include c-Myb synaptophysin group 1 glutamate receptors promyelocytic leukemia protein alpha-synuclein synphilin-1 and alpha-ketoglutarate dehydrogenase among others. Sina proteins also bind proteins that are not targets for ubiquitination such as Phyl adenomatous polyposis coli VAV BAG-1 and Dab-1. Siah binds to a consensus motif PXAXVXP which is present in Siah-binding proteins. Siah is a dimeric protein consisting of an N-terminal RING domain two zinc finger motifs and a C-terminal substrate-binding domain (SBD) this SBD contains an eight-stranded antiparallel beta-sandwich fold similar to the MATH (meprin and TRAF-C homology) domain..
rpsblast_kog gnl|CDD|38212 1 305 + 305 Gaps:10 99.33 299 40.40 1e-82 KOG3002 KOG3002 KOG3002 Zn finger protein [General function prediction only].

9 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Gene3D 123 165 43 G3DSA:3.90.890.10 none none IPR013323
SUPERFAMILY 49 97 49 SSF57850 none none none
ProSiteProfiles 57 93 37 PS50089 none Zinc finger RING-type profile. IPR001841
ProSiteProfiles 110 170 61 PS51081 "UniPathway:UPA00143" Zinc finger SIAH-type profile. IPR013010
Gene3D 176 302 127 G3DSA:2.60.210.10 none none none
SUPERFAMILY 110 302 193 SSF49599 none none IPR008974
Pfam 99 298 200 PF03145 "UniPathway:UPA00143" Seven in absentia protein family IPR018121
Gene3D 45 122 78 G3DSA:3.30.40.10 none none IPR013083
PANTHER 39 305 267 PTHR10315 "UniPathway:UPA00143";signature_desc=SEVEN IN ABSENTIA HOMOLOG none IPR004162

0 Localization

0 Qtllist

0 Targeting