Protein : Qrob_P0007910.2 Q. robur
Protein Identifier  ? Qrob_P0007910.2 Organism . Name  Quercus robur
Score  93.3 Score Type  egn
Protein Description  (M=5) 1.16.5.1 - Ascorbate ferrireductase (transmembrane). Code Enzyme  EC:1.16.5.1
Gene Prediction Quality  validated Protein length 

Sequence

Length: 210  
Kegg Orthology  K08360
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0 Synonyms

1 GO Terms

Identifier Name Description
GO:0016021 integral component of membrane The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.

31 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|fve:101295085 4 196 + 193 Gaps:10 79.57 230 73.22 4e-88 transmembrane ascorbate ferrireductase 1-like
blastp_kegg lcl|pop:POPTR_0015s13160g 5 196 + 192 Gaps:10 86.67 210 73.63 2e-86 POPTRDRAFT_824927 hypothetical protein
blastp_kegg lcl|pop:POPTR_0012s13210g 8 196 + 189 Gaps:10 65.57 273 73.74 1e-84 POPTRDRAFT_662338 hypothetical protein
blastp_kegg lcl|tcc:TCM_015161 4 196 + 193 Gaps:10 80.26 228 74.86 4e-84 Cytochrome b561/ferric reductase transmembrane protein family isoform 1
blastp_kegg lcl|pxb:103960561 4 196 + 193 Gaps:12 79.74 232 69.19 1e-83 transmembrane ascorbate ferrireductase 1-like
blastp_kegg lcl|pxb:103940888 4 196 + 193 Gaps:12 79.74 232 69.19 1e-83 transmembrane ascorbate ferrireductase 1-like
blastp_kegg lcl|mdm:103439749 4 196 + 193 Gaps:11 79.65 231 69.02 5e-83 transmembrane ascorbate ferrireductase 1
blastp_kegg lcl|cic:CICLE_v10005795mg 4 194 + 191 Gaps:10 78.70 230 77.35 8e-83 hypothetical protein
blastp_kegg lcl|cit:102608192 4 194 + 191 Gaps:10 78.70 230 77.35 8e-83 transmembrane ascorbate ferrireductase 1-like
blastp_kegg lcl|cam:101500328 8 195 + 188 Gaps:10 77.73 229 70.22 3e-81 transmembrane ascorbate ferrireductase 1-like
blastp_uniprot_sprot sp|Q8L856|ACFR1_ARATH 4 202 + 199 Gaps:16 81.59 239 63.08 1e-75 Transmembrane ascorbate ferrireductase 1 OS Arabidopsis thaliana GN CYB561A PE 1 SV 1
blastp_uniprot_sprot sp|A3A9H6|ACET1_ORYSJ 4 196 + 193 Gaps:11 78.63 234 57.61 7e-67 Probable ascorbate-specific transmembrane electron transporter 1 OS Oryza sativa subsp. japonica GN Os02g0642300 PE 3 SV 1
blastp_uniprot_sprot sp|Q6I681|ACET1_MAIZE 4 195 + 192 Gaps:11 77.54 236 56.83 7e-65 Ascorbate-specific transmembrane electron transporter 1 OS Zea mays GN ZCYB PE 1 SV 1
blastp_uniprot_sprot sp|C4IYS8|ACFR2_MAIZE 4 193 + 190 Gaps:11 76.69 236 57.46 2e-64 Ascorbate-specific transmembrane electron transporter 2 OS Zea mays PE 2 SV 1
blastp_uniprot_sprot sp|Q7XMK3|ACET2_ORYSJ 4 172 + 169 Gaps:11 67.80 236 60.00 3e-58 Probable ascorbate-specific transmembrane electron transporter 2 OS Oryza sativa subsp. japonica GN Os04g0533500 PE 2 SV 2
blastp_uniprot_sprot sp|Q9SWS1|ACFR2_ARATH 8 200 + 193 Gaps:13 79.13 230 39.01 2e-41 Probable transmembrane ascorbate ferrireductase 2 OS Arabidopsis thaliana GN CYB561B PE 2 SV 1
blastp_uniprot_sprot sp|Q9C540|ACFR4_ARATH 9 207 + 199 Gaps:17 81.36 236 38.54 6e-33 Probable transmembrane ascorbate ferrireductase 4 OS Arabidopsis thaliana GN CYB561D PE 2 SV 1
blastp_uniprot_sprot sp|Q95245|CY561_PIG 9 206 + 198 Gaps:20 76.19 252 35.94 6e-31 Cytochrome b561 OS Sus scrofa GN CYB561 PE 2 SV 1
blastp_uniprot_sprot sp|Q60720|CY561_MOUSE 4 206 + 203 Gaps:20 78.80 250 34.01 2e-30 Cytochrome b561 OS Mus musculus GN Cyb561 PE 2 SV 2
blastp_uniprot_sprot sp|Q67ZF6|ACFR3_ARATH 7 185 + 179 Gaps:14 73.66 224 38.18 8e-30 Probable transmembrane ascorbate ferrireductase 3 OS Arabidopsis thaliana GN CYB561C PE 2 SV 1
rpsblast_cdd gnl|CDD|178406 4 200 + 197 Gaps:10 80.95 231 71.66 3e-72 PLN02810 PLN02810 carbon-monoxide oxygenase.
rpsblast_cdd gnl|CDD|176496 7 153 + 147 Gaps:10 95.14 144 55.47 1e-48 cd08766 Cyt_b561_ACYB-1_like Plant cytochrome b(561) including the carbon monoxide oxygenase ACYB-1. Cytochrome b(561) as found in plants similar to the Arabidopsis thaliana ACYB-1 gene product a cytochrome b561 isoform localized to the tonoplast. This protein might act as a ferric-chelate reductase catalyzing the reduction of Fe(3+) to Fe(2+) and might be capable of trans-membrane electron transport from intracellular ascorbate to extracellular ferric chelates. It is assumed that this protein uses ascorbate as the electron donor. Belongs to the cytochrome b(561) family which are secretory vesicle-specific electron transport proteins. Cytochromes b(561) are integral membrane proteins that bind two heme groups non-covalently and may have six alpha-helical trans-membrane segments.
rpsblast_cdd gnl|CDD|178284 9 194 + 186 Gaps:11 76.29 232 40.68 2e-39 PLN02680 PLN02680 carbon-monoxide oxygenase.
rpsblast_cdd gnl|CDD|190557 9 152 + 144 Gaps:13 100.00 137 52.55 5e-36 pfam03188 Cytochrom_B561 Eukaryotic cytochrome b561. Cytochrome b561 is a secretory vesicle-specific electron transport protein. It is an integral membrane protein that binds two heme groups non-covalently. This is a eukaryotic family. Members of the 'prokaryotic cytochrome b561' family can be found in Pfam: PF01292.
rpsblast_cdd gnl|CDD|177985 9 187 + 179 Gaps:14 69.83 242 44.38 3e-30 PLN02351 PLN02351 cytochromes b561 family protein.
rpsblast_cdd gnl|CDD|128911 9 145 + 137 Gaps:12 100.00 129 46.51 3e-29 smart00665 B561 Cytochrome b-561 / ferric reductase transmembrane domain. Cytochrome b-561 recycles ascorbate for the generation of norepinephrine by dopamine-beta-hydroxylase in the chromaffin vesicles of the adrenal gland. It is a transmembrane heme protein with the two heme groups being bound to conserved histidine residues. A cytochrome b-561 homologue termed Dcytb is an iron-regulated ferric reductase in the duodenal mucosa. Other homologues of these are also likely to be ferric reductases. SDR2 is proposed to be important in regulating the metabolism of iron in the onset of neurodegenerative disorders.
rpsblast_cdd gnl|CDD|176494 10 182 + 173 Gaps:20 77.10 214 40.61 8e-28 cd08764 Cyt_b561_CG1275_like Non-vertebrate eumetazoan cytochrome b(561). Cytochrome b(561) as found in non-vertebrate eumetazoans similar to the Drosophila melanogaster CG1275 gene product. This protein might act as a ferric-chelate reductase catalyzing the reduction of Fe(3+) to Fe(2+) such as associated with the transport of iron from the endosome to the cytoplasm. It is assumed that this protein uses ascorbate as the electron donor. Belongs to the cytochrome b(561) family which are secretory vesicle-specific electron transport proteins. Cytochromes b(561) are integral membrane proteins that bind two heme groups non-covalently and may have six alpha-helical trans-membrane segments.
rpsblast_cdd gnl|CDD|176493 10 151 + 142 Gaps:15 93.01 143 42.86 3e-23 cd08763 Cyt_b561_CYB561 Vertebrate cytochrome b(561) CYB561 gene product. Cytochrome b(561) as found in vertebrates which might act as a ferric-chelate reductase catalyzing the reduction of Fe(3+) to Fe(2+) such as associated with the transport of iron from the endosome to the cytoplasm. It is assumed that this protein uses ascorbate as the electron donor. Belongs to the cytochrome b(561) family which are secretory vesicle-specific electron transport proteins. Cytochromes b(561) are integral membrane proteins that bind two heme groups non-covalently and may have six alpha-helical trans-membrane segments.
rpsblast_cdd gnl|CDD|176489 7 138 + 132 Gaps:11 93.89 131 44.72 1e-22 cd08554 Cyt_b561 Eukaryotic cytochrome b(561). Cytochrome b(561) is a family of endosomal or secretory vesicle-specific electron transport proteins. They are integral membrane proteins that bind two heme groups non-covalently and may have six alpha-helical trans-membrane segments. This is an exclusively eukaryotic family. Members of the prokaryotic cytochrome b561 family are not deemed homologous.
rpsblast_cdd gnl|CDD|176495 10 151 + 142 Gaps:15 88.24 153 40.74 5e-19 cd08765 Cyt_b561_CYBRD1 Vertebrate cytochrome b(561) CYBRD1 gene product. Duodenal cytochrome b or ferric-chelate reductase 3 a cytochrome b(561) as found in vertebrates which might act as a ferric-chelate reductase catalyzing the reduction of Fe(3+) to Fe(2+) such as associated with the transport of iron from the endosome to the cytoplasm. It is assumed that this protein uses ascorbate as the electron donor. This protein is expressed at the brush border of duodenal enterocytes and may play a role in the uptake of dietary Fe(3+) facilitating its transport into the mucosal cells. It may also be involved in the recycling of extracellular ascorbate in erythrocyte membranes and act as a ferrireductase in epithelial cells of the respiratory system. Belongs to the cytochrome b(561) family which are secretory vesicle-specific electron transport proteins. Cytochromes b(561) are integral membrane proteins that bind two heme groups non-covalently and may have six alpha-helical trans-membrane segments.

18 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Phobius 12 32 21 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 91 101 11 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
ProSiteProfiles 1 186 186 PS50939 none Cytochrome b561 domain profile. IPR006593
Phobius 187 209 23 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 147 165 19 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
PANTHER 7 195 189 PTHR10106 none none none
PANTHER 7 195 189 PTHR10106:SF9 none none none
Phobius 65 69 5 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 120 124 5 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 70 90 21 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 44 64 21 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 166 186 21 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Pfam 9 151 143 PF03188 none Eukaryotic cytochrome b561 IPR004877
Phobius 102 119 18 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
SMART 9 145 137 SM00665 none Cytochrome b-561 / ferric reductase transmembrane domain. IPR006593
Phobius 1 11 11 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 33 43 11 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 125 146 22 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none

6 Localization

Analysis Start End Length
TMHMM 44 62 18
TMHMM 7 29 22
TMHMM 101 118 17
TMHMM 123 145 22
TMHMM 165 187 22
TMHMM 69 91 22

3 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran1_2003_QTL2_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 87 lod 3,3 8,7
Bourran2_2004_QTL9_peak_Bud_burst_3P Qrob_Chr02 2 s_1C34E9_788 v_12238_322 50 25 75 lod 4,4 10,1
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL2_d13Cf Qrob_Chr02 2 s_1AQA4Z_1644 s_1AK5QX_947 53.67 14,01 79,68 lod 5.6594 0.03

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 25   Secretory pathway 1 0.942 0.033 NON-PLANT 25