| Protocol |
Molecular weigth: null | Lib seq origin: null | Definition: Wheat meiotic anther cDNA library | Target genes: null | nb seq planned: null (5') null (3') | Labo: E. coli DH10B | lib orientation: null | lib primed: null | Plants were grown in a glasshouse. Anther meiotic stage was determined by removing anthers from individual primary florets. One anther was sacrificed for microscopic staging, and if determined to be between (and including) meiotic stages pre-meiosis and metaphase I, the remaining two anthers were collected and pooled for library construction. The tissue, total RNA and poly(A) RNA were prepared, cDNA synthesised and directionally ligated into pSPORT1 by Tim Sutton in the P Langridge Lab at the Department of Plant Science, University of Adelaide, Waite Campus, Australia. Average insert size 1.5Kb. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). | comment: null |
