| Protocol |
Molecular weigth: null | Lib seq origin: null | Definition: Aegilops speltoides pre-meiotic anther cDNA library | Target genes: null | nb seq planned: null (5') null (3') | Labo: E. coli SOLR | lib orientation: null | lib primed: null | Plants were grown in a growth chamber at the University of California, Davis (Akhunov in Devorak's lab). Pre-meiotic anthers were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made and the cDNA clones were in vivo excised to give pBluescript phagemids at the University of California, Davis (Akhunov in Devorak's lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). | comment: null |
