| Protocol |
Molecular weigth: null | Lib seq origin: null | Definition: Triticum monococcum vernalized apex cDNA library | Target genes: null | nb seq planned: null (5') null (3') | Labo: E. coli XLOLR | lib orientation: null | lib primed: null | One-month old plants were subjected to vernalization treatment by placing them in the cold room at 6 C, under 15hr light/9hr dark condition. Total RNA was prepared from apex tissue extracted from plants with no cold treatment, and from plants with 2-week, 4-week and 6-week cold treatment separately. Equal amount of total RNA was pooled from all four samples, a cDNA library was made using pooled polyA RNA and cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). | comment: null |
