| Protocol |
Molecular weigth: null | Lib seq origin: null | Definition: Wheat CS whole plant cDNA library | Target genes: null | nb seq planned: null (5') null (3') | Labo: E. coli SOLR | lib orientation: null | lib primed: null | Plant tissues from wheat cv. CS grown to full tillering stage in greenhouse were collected at University of California, Davis (Jan Dvorak's lab, K. Deal and E. Akhunov ). Total RNA was prepared from leaves (young leaf and third leaf), whole roots, crown, steam and sheath tissues and then equal quantities of RNA were pooled from the these samples. PolyA was purified from the pooled RNA, a cDNA library was made and the cDNA clones were in vivo excised to give pBluescript SK(-) phagemids in J. Dvorak's lab (E. Akhunov, J. Dvorak) at the University of California, Davis. Colony plating, plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). | comment: null |
