| Protocol |
Molecular weigth: null | Lib seq origin: null | Definition: CS wheat 5-15 DAP spike cDNA library | Target genes: null | nb seq planned: null (5') null (3') | Labo: E. coli SOLR | lib orientation: null | lib primed: null | Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). | comment: null |
