| Protocol |
Molecular weigth: null | Lib seq origin: null | Definition: CS wheat unstressed etiolated seedling shoot cDNA library | Target genes: null | nb seq planned: null (5') null (3') | Labo: E. coli SOLR | lib orientation: null | lib primed: null | Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA and poly(A) RNA were prepared, a cDNA library was made and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). | comment: null |
