GRS - Genome Report System

Sclerotinia sclerotiorum GRS

Functional annotation

SS1G_12843.1

Gene Identifier

Domain/Motif (Interproscan results)

Localization / Targeting

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	ssl:SS1G_12843	1	518	518	n/a	100.00	518	100.00	0.00	0.0	similar to translation initiation factor 2 gamma subunit K03242 translation initiation factor eIF-2 gamma subunit
	bfu:BC1G_16041	1	518	518	n/a	100.00	518	98.46	0.77	0.0	similar to translation initiation factor 2 gamma subunit K03242 translation initiation factor eIF-2 gamma subunit
	fgr:FG06843.1	1	518	518	Gaps:9	100.00	509	85.07	7.47	0.0	hypothetical protein K03242 translation initiation factor eIF-2 gamma subunit
	mgr:MGG_01592	3	518	516	Gaps:4	99.81	513	84.38	8.20	0.0	MG01592.4 similar to translation initiation factor 2 gamma subunit K03242 translation initiation factor eIF-2 gamma subunit
	ncr:NCU02810	1	509	509	Gaps:9	100.00	502	86.85	7.57	0.0	eukaryotic translation initiation factor 2 gamma subunit K03242 translation initiation factor eIF-2 gamma subunit
	pan:PODANSg10065	1	518	518	Gaps:9	100.00	509	84.87	9.04	0.0	hypothetical protein K03242 translation initiation factor eIF-2 gamma subunit
	nfi:NFIA_108620	1	515	515	Gaps:12	99.42	516	80.31	8.97	0.0	translation initiation factor EF-2 gamma subunit putative K03242 translation initiation factor eIF-2 gamma subunit
	afm:AFUA_4G07580	1	515	515	Gaps:12	99.42	516	80.12	8.97	0.0	translation initiation factor EF-2 gamma subunit putative K03242 translation initiation factor eIF-2 gamma subunit
	ang:An04g01940	1	515	515	Gaps:13	99.42	513	80.78	8.04	0.0	hypothetical protein K03242 translation initiation factor eIF-2 gamma subunit
	afv:AFLA_112270	1	515	515	Gaps:13	99.42	513	80.20	8.43	0.0	translation initiation factor EF-2 gamma subunit putative K03242 translation initiation factor eIF-2 gamma subunit
blastp_uniprot_sprot	sp\|P32481\|IF2G_YEAST	63	515	453	Gaps:2	85.58	527	77.38	10.86	0.0	Eukaryotic translation initiation factor 2 subunit gamma OS Saccharomyces cerevisiae GN GCD11 PE 1 SV 1
	sp\|Q09130\|IF2G_SCHPO	70	513	444	Gaps:2	99.10	446	75.57	11.31	0.0	Eukaryotic translation initiation factor 2 subunit gamma OS Schizosaccharomyces pombe GN tif213 PE 2 SV 1
	sp\|Q24208\|IF2G_DROME	71	515	445	Gaps:1	93.89	475	71.08	13.23	0.0	Eukaryotic translation initiation factor 2 subunit 3 OS Drosophila melanogaster GN Su(var)3-9 PE 2 SV 1
	sp\|Q54XD8\|IF2G_DICDI	70	513	444	n/a	96.52	460	69.82	14.19	0.0	Eukaryotic translation initiation factor 2 subunit 3 OS Dictyostelium discoideum GN eif2s3 PE 2 SV 1
	sp\|Q2KHU8\|IF2G_BOVIN	72	516	445	Gaps:1	94.49	472	70.40	14.35	0.0	Eukaryotic translation initiation factor 2 subunit 3 OS Bos taurus GN EIF2S3 PE 2 SV 1
	sp\|P41091\|IF2G_HUMAN	72	516	445	Gaps:1	94.49	472	70.40	14.35	0.0	Eukaryotic translation initiation factor 2 subunit 3 OS Homo sapiens GN EIF2S3 PE 1 SV 3
	sp\|P81795\|IF2G_RAT	72	516	445	Gaps:1	94.49	472	70.40	14.35	0.0	Eukaryotic translation initiation factor 2 subunit 3 OS Rattus norvegicus GN Eif2s3 PE 1 SV 2
	sp\|Q9Z0N1\|IF2G_MOUSE	72	516	445	Gaps:1	94.49	472	70.40	14.35	0.0	Eukaryotic translation initiation factor 2 subunit 3 X-linked OS Mus musculus GN Eif2s3x PE 1 SV 2
	sp\|Q9Z0N2\|IF2H_MOUSE	72	516	445	Gaps:1	94.49	472	70.63	13.90	0.0	Eukaryotic translation initiation factor 2 subunit 3 Y-linked OS Mus musculus GN Eif2s3y PE 2 SV 2
	sp\|Q5R797\|IF2G_PONAB	72	516	445	Gaps:1	94.49	472	70.18	14.35	0.0	Eukaryotic translation initiation factor 2 subunit 3 OS Pongo abelii GN EIF2S3 PE 2 SV 1
blastp_pdb	1s0u_A	85	507	423	Gaps:19	99.02	408	50.25	19.31	1e-108	mol:protein length:408 Translation initiation factor 2 gamma subunit
	2dcu_A	86	507	422	Gaps:19	96.18	419	47.39	21.34	1e-101	mol:protein length:419 Translation initiation factor 2 gamma subunit
	2d74_A	86	507	422	Gaps:19	96.18	419	47.39	21.34	1e-101	mol:protein length:419 Translation initiation factor 2 gamma subunit
	1kk3_A	86	507	422	Gaps:19	98.29	410	47.39	21.34	1e-100	mol:protein length:410 eIF2gamma
	1kk0_A	86	507	422	Gaps:19	98.29	410	47.39	21.34	1e-100	mol:protein length:410 eIF2gamma
	1kk2_A	86	507	422	Gaps:19	98.29	410	47.15	21.34	1e-99	mol:protein length:410 eIF2gamma
	1kk1_A	86	507	422	Gaps:19	98.29	410	47.15	21.34	1e-99	mol:protein length:410 eIF2gamma
	1kjz_A	86	507	422	Gaps:19	98.29	410	47.15	21.34	1e-99	mol:protein length:410 eIF2gamma
	3i1f_B	88	507	420	Gaps:15	98.07	415	43.73	21.87	5e-95	mol:protein length:415 Translation initiation factor 2 subunit gamma
	3i1f_A	88	507	420	Gaps:15	98.07	415	43.73	21.87	5e-95	mol:protein length:415 Translation initiation factor 2 subunit gamma
rpsblast_cdd	gnl\|CDD\|173526	68	513	446	Gaps:7	95.70	465	68.54	12.81	0.0	PTZ00327 PTZ00327 eukaryotic translation initiation factor 2 gamma subunit Provisional.
	gnl\|CDD\|34854	86	510	425	Gaps:16	98.55	415	54.28	16.87	1e-163	COG5257 GCD11 Translation initiation factor 2 gamma subunit (eIF-2gamma GTPase) [Translation ribosomal structure and biogenesis].
	gnl\|CDD\|167667	87	507	421	Gaps:16	98.30	412	49.88	20.25	1e-144	PRK04000 PRK04000 translation initiation factor IF-2 subunit gamma Validated.
	gnl\|CDD\|163393	87	507	421	Gaps:18	99.75	406	50.62	19.26	1e-133	TIGR03680 eif2g_arch translation initiation factor 2 subunit gamma. eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.
	gnl\|CDD\|133288	91	297	207	Gaps:4	100.00	203	73.40	11.33	1e-109	cd01888 eIF2_gamma eIF2-gamma (gamma subunit of initiation factor 2). eIF2 is a heterotrimeric translation initiation factor that consists of alpha beta and gamma subunits. The GTP-bound gamma subunit also binds initiator methionyl-tRNA and delivers it to the 40S ribosomal subunit. Following hydrolysis of GTP to GDP eIF2:GDP is released from the ribosome. The gamma subunit has no intrinsic GTPase activity but is stimulated by the GTPase activating protein (GAP) eIF5 and GDP/GTP exchange is stimulated by the guanine nucleotide exchange factor (GEF) eIF2B. eIF2B is a heteropentamer and the epsilon chain binds eIF2. Both eIF5 and eIF2B-epsilon are known to bind strongly to eIF2-beta but have also been shown to bind directly to eIF2-gamma. It is possible that eIF2-beta serves simply as a high-affinity docking site for eIF5 and eIF2B-epsilon or that eIF2-beta serves a regulatory role. eIF2-gamma is found only in eukaryotes and archaea. It is closely related to SelB the selenocysteine-specific elongation factor from eubacteria. The translational factor components of the ternary complex IF2 in eubacteria and eIF2 in eukaryotes are not the same protein (despite their unfortunately similar names). Both factors are GTPases however eubacterial IF-2 is a single polypeptide while eIF2 is heterotrimeric. eIF2-gamma is a member of the same family as eubacterial IF2 but the two proteins are only distantly related. This family includes translation initiation elongation and release factors.
	gnl\|CDD\|58079	298	411	114	Gaps:1	100.00	113	71.68	17.70	7e-47	cd03688 eIF2_gamma_II eIF2_gamma_II: this subfamily represents the domain II of the gamma subunit of eukaryotic translation initiation factor 2 (eIF2-gamma) found in Eukaryota and Archaea. eIF2 is a G protein that delivers the methionyl initiator tRNA to the small ribosomal subunit and releases it upon GTP hydrolysis after the recognition of the initiation codon. eIF2 is composed three subunits alpha beta and gamma. Subunit gamma shows strongest conservation and it confers both tRNA binding and GTP/GDP binding..
	gnl\|CDD\|150009	417	507	91	Gaps:3	100.00	88	64.77	12.50	7e-36	pfam09173 eIF2_C Initiation factor eIF2 gamma C terminal. Members of this family which are found in the initiation factors eIF2 and EF-Tu adopt a structure consisting of a beta barrel with Greek key topology. They are required for formation of the ternary complex with GTP and initiator tRNA.
	gnl\|CDD\|143801	91	294	204	Gaps:56	98.38	185	34.07	17.58	3e-35	pfam00009 GTP_EFTU Elongation factor Tu GTP binding domain. This domain contains a P-loop motif also found in several other families such as pfam00071 pfam00025 and pfam00063. Elongation factor Tu consists of three structural domains this plus two C-terminal beta barrel domains.
	gnl\|CDD\|133371	91	290	200	Gaps:37	99.39	164	36.81	23.31	4e-30	cd04171 SelB SelB subfamily. SelB is an elongation factor needed for the co-translational incorporation of selenocysteine. Selenocysteine is coded by a UGA stop codon in combination with a specific downstream mRNA hairpin. In bacteria the C-terminal part of SelB recognizes this hairpin while the N-terminal part binds GTP and tRNA in analogy with elongation factor Tu (EF-Tu). It specifically recognizes the selenocysteine charged tRNAsec which has a UCA anticodon in an EF-Tu like manner. This allows insertion of selenocysteine at in-frame UGA stop codons. In E. coli SelB binds GTP selenocysteyl-tRNAsec and a stem-loop structure immediately downstream of the UGA codon (the SECIS sequence). The absence of active SelB prevents the participation of selenocysteyl-tRNAsec in translation. Archaeal and animal mechanisms of selenocysteine incorporation are more complex. Although the SECIS elements have different secondary structures and conserved elements between archaea and eukaryotes they do share a common feature. Unlike in E. coli these SECIS elements are located in the 3' UTRs. This group contains bacterial SelBs as well as one from archaea.
	gnl\|CDD\|133257	92	295	204	Gaps:63	100.00	189	31.75	16.93	2e-24	cd00881 GTP_translation_factor GTP translation factor family. This family consists primarily of translation initiation elongation and release factors which play specific roles in protein translation. In addition the family includes Snu114p a component of the U5 small nuclear riboprotein particle which is a component of the spliceosome and is involved in excision of introns TetM a tetracycline resistance gene that protects the ribosome from tetracycline binding and the unusual subfamily CysN/ATPS which has an unrelated function (ATP sulfurylase) acquired through lateral transfer of the EF1-alpha gene and development of a new function.
rpsblast_kog	gnl\|CDD\|35687	68	515	448	Gaps:1	96.35	466	73.27	13.81	0.0	KOG0466 KOG0466 KOG0466 Translation initiation factor 2 gamma subunit (eIF-2gamma GTPase) [Translation ribosomal structure and biogenesis].
	gnl\|CDD\|35681	91	429	339	Gaps:69	67.71	449	31.91	17.11	1e-22	KOG0460 KOG0460 KOG0460 Mitochondrial translation elongation factor Tu [Translation ribosomal structure and biogenesis].
	gnl\|CDD\|35682	86	381	296	Gaps:53	51.15	522	29.96	20.22	8e-21	KOG0461 KOG0461 KOG0461 Selenocysteine-specific elongation factor [Translation ribosomal structure and biogenesis].
	gnl\|CDD\|35683	66	343	278	Gaps:69	36.15	650	29.79	17.02	1e-10	KOG0462 KOG0462 KOG0462 Elongation factor-type GTP-binding protein [Translation ribosomal structure and biogenesis].
	gnl\|CDD\|35679	2	399	398	Gaps:89	63.18	603	22.05	17.06	1e-09	KOG0458 KOG0458 KOG0458 Elongation factor 1 alpha [Translation ribosomal structure and biogenesis].
	gnl\|CDD\|35680	29	408	380	Gaps:63	72.85	501	23.01	19.18	2e-07	KOG0459 KOG0459 KOG0459 Polypeptide release factor 3 [Translation ribosomal structure and biogenesis].

Other analysis

Functional Gbrowse

Ortholog