GRS - Genome Report System

Sclerotinia sclerotiorum GRS

Functional annotation

SS1G_09546.1

Gene Identifier

Domain/Motif (Interproscan results)

Localization / Targeting

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	ssl:SS1G_09546	1	298	298	n/a	100.00	298	100.00	0.00	1e-177	hypothetical protein
	bfu:BC1G_14083	8	262	255	n/a	21.48	1187	89.41	6.27	1e-138	nitrilase
	aor:AO090102000347	8	276	269	Gaps:18	97.55	286	53.41	19.35	2e-83	hypothetical protein
	act:ACLA_002910	10	276	267	Gaps:11	90.34	290	55.34	18.32	6e-82	hydrolase carbon-nitrogen family putative
	afm:AFUA_5G02350	10	276	267	Gaps:17	83.49	321	55.97	15.67	2e-79	hydrolase carbon-nitrogen family
	pcs:Pc21g15050	8	276	269	Gaps:11	90.72	291	52.27	20.45	6e-79	Pc21g15050
	nfi:NFIA_039740	10	276	267	Gaps:17	87.87	305	54.85	16.42	1e-78	hydrolase carbon-nitrogen family putative
	ani:AN8024.2	7	276	270	Gaps:26	91.75	303	50.72	17.99	3e-77	hypothetical protein
	pno:SNOG_08320	8	267	260	Gaps:41	95.35	301	53.31	11.85	1e-76	hypothetical protein
	nfi:NFIA_007720	4	289	286	Gaps:27	94.21	311	50.17	13.99	5e-73	hydrolase carbon-nitrogen family protein
blastp_uniprot_sprot	sp\|O31664\|MTNU_BACSU	1	267	267	Gaps:24	93.82	259	32.10	16.46	3e-17	UPF0012 hydrolase mtnU OS Bacillus subtilis GN mtnU PE 3 SV 1
	sp\|Q9NQR4\|NIT2_HUMAN	10	259	250	Gaps:29	85.87	276	29.11	20.25	2e-15	Omega-amidase NIT2 OS Homo sapiens GN NIT2 PE 1 SV 1
	sp\|P49954\|NIT3_YEAST	10	260	251	Gaps:25	84.54	291	28.46	20.33	3e-14	Probable hydrolase NIT3 OS Saccharomyces cerevisiae GN NIT3 PE 1 SV 1
	sp\|O59829\|YCU9_SCHPO	64	252	189	Gaps:22	62.87	272	31.58	22.22	1e-13	Probable nitrilase C965.09 OS Schizosaccharomyces pombe GN SPCC965.09 PE 2 SV 1
	sp\|Q9JHW2\|NIT2_MOUSE	10	259	250	Gaps:35	85.87	276	27.85	21.10	1e-13	Omega-amidase NIT2 OS Mus musculus GN Nit2 PE 1 SV 1
	sp\|Q6INI7\|NIT2B_XENLA	10	264	255	Gaps:35	87.68	276	26.86	21.90	1e-12	Omega-amidase NIT2-B OS Xenopus laevis GN nit2b PE 2 SV 1
	sp\|Q5R4L6\|NIT2_PONAB	10	259	250	Gaps:36	85.82	275	27.97	20.34	1e-12	Omega-amidase NIT2 OS Pongo abelii GN NIT2 PE 3 SV 1
	sp\|Q6IR61\|NIT2A_XENLA	10	259	250	Gaps:39	85.87	276	27.85	21.94	2e-12	Omega-amidase NIT2-A OS Xenopus laevis GN nit2a PE 2 SV 1
	sp\|Q28IE5\|NIT2_XENTR	10	264	255	Gaps:35	87.68	276	26.03	21.49	2e-12	Omega-amidase NIT2 OS Xenopus tropicalis GN nit2 PE 2 SV 1
	sp\|P55175\|Y601_SYNY3	10	259	250	Gaps:36	88.97	272	27.27	21.07	2e-12	UPF0012 hydrolase sll0601 OS Synechocystis sp. (strain PCC 6803) GN sll0601 PE 3 SV 1
blastp_pdb	1f89_B	10	260	251	Gaps:25	84.54	291	28.46	20.33	5e-15	mol:protein length:291 32.5 KDA PROTEIN YLR351C
	1f89_A	10	260	251	Gaps:25	84.54	291	28.46	20.33	5e-15	mol:protein length:291 32.5 KDA PROTEIN YLR351C
	2w1v_B	10	259	250	Gaps:35	85.87	276	27.85	21.10	2e-14	mol:protein length:276 NITRILASE HOMOLOG 2
	2w1v_A	10	259	250	Gaps:35	85.87	276	27.85	21.10	2e-14	mol:protein length:276 NITRILASE HOMOLOG 2
	1j31_D	8	253	246	Gaps:29	85.11	262	30.49	16.59	5e-13	mol:protein length:262 Hypothetical protein PH0642
	1j31_C	8	253	246	Gaps:29	85.11	262	30.49	16.59	5e-13	mol:protein length:262 Hypothetical protein PH0642
	1j31_B	8	253	246	Gaps:29	85.11	262	30.49	16.59	5e-13	mol:protein length:262 Hypothetical protein PH0642
	1j31_A	8	253	246	Gaps:29	85.11	262	30.49	16.59	5e-13	mol:protein length:262 Hypothetical protein PH0642
	3hkx_A	17	252	236	Gaps:33	77.39	283	29.68	18.26	2e-12	mol:protein length:283 Amidase
	3iw3_B	8	254	247	Gaps:29	85.50	262	29.02	17.41	2e-11	mol:protein length:262 Nitrilase
rpsblast_cdd	gnl\|CDD\|143587	8	266	259	Gaps:23	94.07	253	34.87	15.55	1e-50	cd07197 nitrilase Nitrilase superfamily including nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes. This superfamily (also known as the C-N hydrolase superfamily) contains hydrolases that break carbon-nitrogen bonds it includes nitrilases cyanide dihydratases aliphatic amidases N-terminal amidases beta-ureidopropionases biotinidases pantotheinase N-carbamyl-D-amino acid amidohydrolases the glutaminase domain of glutamine-dependent NAD+ synthetase apolipoprotein N-acyltransferases and N-carbamoylputrescine amidohydrolases among others. These enzymes depend on a Glu-Lys-Cys catalytic triad and work through a thiol acylenzyme intermediate. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer. These oligomers include dimers tetramers hexamers octamers tetradecamers octadecamers as well as variable length helical arrangements and homo-oligomeric spirals. These proteins have roles in vitamin and co-enzyme metabolism in detoxifying small molecules in the synthesis of signaling molecules and in the post-translational modification of proteins. They are used industrially as biocatalysts in the fine chemical and pharmaceutical industry in cyanide remediation and in the treatment of toxic effluent. This superfamily has been classified previously in the literature based on global and structure-based sequence analysis into thirteen different enzyme classes (referred to as 1-13). This hierarchy includes those thirteen classes and a few additional subfamilies. A putative distant relative the plasmid-borne TraB family has not been included in the hierarchy.
	gnl\|CDD\|143605	10	260	251	Gaps:26	91.37	255	30.90	16.74	1e-32	cd07581 nitrilase_3 Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases). The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
	gnl\|CDD\|30737	8	266	259	Gaps:27	89.05	274	32.79	16.80	2e-31	COG0388 COG0388 Predicted amidohydrolase [General function prediction only].
	gnl\|CDD\|143607	8	257	250	Gaps:34	90.12	253	33.33	20.18	2e-28	cd07583 nitrilase_5 Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases). The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
	gnl\|CDD\|143596	10	267	258	Gaps:57	91.70	265	32.51	15.23	1e-26	cd07572 nit Nit1 Nit 2 and related proteins and the Nit1-like domain of NitFhit (class 10 nitrilases). This subgroup includes mammalian Nit1 and Nit2 the Nit1-like domain of the invertebrate NitFhit and various uncharacterized bacterial and archaeal Nit-like proteins. Nit1 and Nit2 are candidate tumor suppressor proteins. In NitFhit the Nit1-like domain is encoded as a fusion protein with the non-homologous tumor suppressor fragile histidine triad (Fhit). Mammalian Nit1 and Fhit may affect distinct signal pathways and both may participate in DNA damage-induced apoptosis. Nit1 is a negative regulator in T cells. Overexpression of Nit2 in HeLa cells leads to a suppression of cell growth through cell cycle arrest in G2. These Nit proteins and the Nit1-like domain of NitFhit belong to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) this subgroup corresponds to class 10.
	gnl\|CDD\|143600	7	261	255	Gaps:33	91.34	254	30.17	16.81	5e-26	cd07576 R-amidase_like Pseudomonas sp. MCI3434 R-amidase and related proteins (putative class 13 nitrilases). Pseudomonas sp. MCI3434 R-amidase hydrolyzes (R S)-piperazine-2-tert-butylcarboxamide to form (R)-piperazine-2-carboxylic acid. It does so with strict R-stereoselectively. Its preferred substrates are carboxamide compounds which have the amino or imino group connected to their beta- or gamma-carbon. This subgroup belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) class 13 represents proteins that at the time were difficult to place in a distinct similarity group. It has been suggested that this subgroup represents a new class. Members of the nitrilase superfamily generally form homomeric complexes the basic building block of which is a homodimer. Native R-amidase however appears to be a monomer.
	gnl\|CDD\|143608	3	252	250	Gaps:44	89.92	258	33.19	12.93	6e-26	cd07584 nitrilase_6 Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases). The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
	gnl\|CDD\|143598	12	273	262	Gaps:33	91.79	280	31.91	14.79	3e-25	cd07574 nitrilase_Rim1_like Uncharacterized subgroup of the nitrilase superfamily some members of this subgroup have an N-terminal RimI domain (class 12 nitrilases). Some members of this subgroup are implicated in post-translational modification as they contain an N-terminal GCN5-related N-acetyltransferase (GNAT) protein RimI family domain. The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) this subgroup corresponds to class 12. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
	gnl\|CDD\|144405	10	165	156	Gaps:9	90.12	172	29.68	20.65	2e-22	pfam00795 CN_hydrolase Carbon-nitrogen hydrolase. This family contains hydrolases that break carbon-nitrogen bonds. The family includes: Nitrilase EC:3.5.5.1 Aliphatic amidase EC:3.5.1.4 Biotidinase EC:3.5.1.12 Beta-ureidopropionase EC:3.5.1.6. Nitrilase-related proteins generally have a conserved E-K-C catalytic triad and are multimeric alpha-beta-beta-alpha sandwich proteins.
	gnl\|CDD\|143604	8	176	169	Gaps:6	61.57	268	33.94	16.97	8e-22	cd07580 nitrilase_2 Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases). The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
rpsblast_kog	gnl\|CDD\|36025	10	256	247	Gaps:36	81.69	295	28.63	17.84	6e-18	KOG0807 KOG0807 KOG0807 Carbon-nitrogen hydrolase [Amino acid transport and metabolism].
	gnl\|CDD\|36024	4	263	260	Gaps:46	86.58	298	21.71	15.50	1e-16	KOG0806 KOG0806 KOG0806 Carbon-nitrogen hydrolase [Amino acid transport and metabolism].
	gnl\|CDD\|36023	12	165	154	Gaps:21	49.55	337	26.35	16.17	1e-07	KOG0805 KOG0805 KOG0805 Carbon-nitrogen hydrolase [Amino acid transport and metabolism].

Other analysis

Functional Gbrowse

Ortholog