GRS - Genome Report System

Sclerotinia sclerotiorum GRS

Functional annotation

SS1G_05107.1

Gene Identifier

Domain/Motif (Interproscan results)

Localization / Targeting

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	ssl:SS1G_05107	21	851	831	n/a	97.65	851	93.98	0.00	0.0	hypothetical protein K12659 N-acetyl-gamma-glutamyl-phosphate reductase / acetylglutamate kinase [EC:1.2.1.38 2.7.2.8]
	bfu:BC1G_07180	26	851	826	n/a	92.39	894	91.65	1.09	0.0	hypothetical protein K12659 N-acetyl-gamma-glutamyl-phosphate reductase / acetylglutamate kinase [EC:1.2.1.38 2.7.2.8]
	ang:An12g07580	26	851	826	Gaps:9	91.58	903	75.57	10.16	0.0	hypothetical protein K12659 N-acetyl-gamma-glutamyl-phosphate reductase / acetylglutamate kinase [EC:1.2.1.38 2.7.2.8]
	act:ACLA_097520	26	851	826	Gaps:10	87.90	942	76.21	8.94	0.0	acetylglutamate kinase putative
	pcs:Pc22g23250	26	851	826	Gaps:5	92.91	888	75.88	8.97	0.0	Pc22g23250
	nfi:NFIA_049240	26	851	826	Gaps:9	91.28	906	75.94	9.07	0.0	acetylglutamate kinase putative K12659 N-acetyl-gamma-glutamyl-phosphate reductase / acetylglutamate kinase [EC:1.2.1.38 2.7.2.8]
	afv:AFLA_016960	26	851	826	Gaps:8	91.39	906	75.24	9.66	0.0	acetylglutamate kinase putative
	afm:AFUA_6G02910	26	851	826	Gaps:7	91.28	906	75.82	8.95	0.0	acetylglutamate kinase (EC:1.2.1.38) K12659 N-acetyl-gamma-glutamyl-phosphate reductase / acetylglutamate kinase [EC:1.2.1.38 2.7.2.8]
	ani:AN8770.2	26	851	826	Gaps:12	91.56	900	75.12	9.83	0.0	hypothetical protein K12659 N-acetyl-gamma-glutamyl-phosphate reductase / acetylglutamate kinase [EC:1.2.1.38 2.7.2.8]
	cim:CIMG_10081	26	851	826	Gaps:13	92.86	882	74.73	9.04	0.0	hypothetical protein
blastp_uniprot_sprot	sp\|P54898\|ARG56_NEUCR	26	849	824	Gaps:22	92.77	871	71.16	12.00	0.0	Protein arg-6 mitochondrial OS Neurospora crassa GN arg-6 PE 1 SV 1
	sp\|P78586\|ARG56_CANAL	26	851	826	Gaps:49	91.83	857	60.74	14.87	0.0	Protein ARG5 6 mitochondrial OS Candida albicans GN ARG5 6 PE 3 SV 1
	sp\|P31318\|ARG56_SCHPO	26	851	826	Gaps:18	91.75	885	53.20	16.26	0.0	Protein arg11 mitochondrial OS Schizosaccharomyces pombe GN arg11 PE 2 SV 2
	sp\|Q01217\|ARG56_YEAST	25	849	825	Gaps:53	90.15	863	57.20	14.40	0.0	Protein ARG5 6 mitochondrial OS Saccharomyces cerevisiae GN ARG5 6 PE 1 SV 1
	sp\|Q54M18\|ARG56_DICDI	43	851	809	Gaps:75	88.31	847	38.24	18.58	1e-128	Bifunctional protein argC mitochondrial OS Dictyostelium discoideum GN argC PE 1 SV 1
	sp\|B2FMD7\|ARGC_STRMK	537	849	313	Gaps:10	97.48	317	54.05	15.86	1e-87	N-acetyl-gamma-glutamyl-phosphate reductase OS Stenotrophomonas maltophilia (strain K279a) GN argC PE 3 SV 1
	sp\|B4SQ93\|ARGC_STRM5	537	849	313	Gaps:10	97.48	317	53.72	15.86	5e-87	N-acetyl-gamma-glutamyl-phosphate reductase OS Stenotrophomonas maltophilia (strain R551-3) GN argC PE 3 SV 1
	sp\|Q8PK31\|ARGC_XANAC	533	848	316	Gaps:10	98.73	316	54.17	15.71	8e-87	N-acetyl-gamma-glutamyl-phosphate reductase OS Xanthomonas axonopodis pv. citri GN argC PE 3 SV 1
	sp\|Q3BSI7\|ARGC_XANC5	537	848	312	Gaps:10	97.47	316	53.90	16.23	7e-86	N-acetyl-gamma-glutamyl-phosphate reductase OS Xanthomonas campestris pv. vesicatoria (strain 85-10) GN argC PE 3 SV 1
	sp\|B0RS54\|ARGC_XANCB	531	848	318	Gaps:12	99.37	316	54.14	14.97	8e-86	N-acetyl-gamma-glutamyl-phosphate reductase OS Xanthomonas campestris pv. campestris (strain B100) GN argC PE 3 SV 1
blastp_pdb	1vkn_D	536	848	313	Gaps:51	94.02	351	35.45	13.03	2e-34	mol:protein length:351 N-acetyl-gamma-glutamyl-phosphate reductase
	1vkn_C	536	848	313	Gaps:51	94.02	351	35.45	13.03	2e-34	mol:protein length:351 N-acetyl-gamma-glutamyl-phosphate reductase
	1vkn_B	536	848	313	Gaps:51	94.02	351	35.45	13.03	2e-34	mol:protein length:351 N-acetyl-gamma-glutamyl-phosphate reductase
	1vkn_A	536	848	313	Gaps:51	94.02	351	35.45	13.03	2e-34	mol:protein length:351 N-acetyl-gamma-glutamyl-phosphate reductase
	2q49_D	524	848	325	Gaps:57	97.49	359	30.29	16.86	4e-32	mol:protein length:359 Probable N-acetyl-gamma-glutamyl-phosphate re
	2q49_C	524	848	325	Gaps:57	97.49	359	30.29	16.86	4e-32	mol:protein length:359 Probable N-acetyl-gamma-glutamyl-phosphate re
	2q49_B	524	848	325	Gaps:57	97.49	359	30.29	16.86	4e-32	mol:protein length:359 Probable N-acetyl-gamma-glutamyl-phosphate re
	2q49_A	524	848	325	Gaps:57	97.49	359	30.29	16.86	4e-32	mol:protein length:359 Probable N-acetyl-gamma-glutamyl-phosphate re
	1xyg_D	524	848	325	Gaps:57	97.49	359	30.29	16.86	4e-32	mol:protein length:359 putative N-acetyl-gamma-glutamyl-phosphate re
	1xyg_C	524	848	325	Gaps:57	97.49	359	30.29	16.86	4e-32	mol:protein length:359 putative N-acetyl-gamma-glutamyl-phosphate re
rpsblast_cdd	gnl\|CDD\|58618	46	294	249	Gaps:1	100.00	248	73.39	11.29	1e-109	cd04252 AAK_NAGK-fArgBP AAK_NAGK-fArgBP: N-Acetyl-L-glutamate kinase (NAGK) of the fungal arginine-biosynthetic pathway (fArgBP). The nuclear-encoded mitochondrial polyprotein precursor with an N-terminal NAGK (ArgB) domain (this CD) a central DUF619 domain and a C-terminal reductase domain (ArgC N-Acetylglutamate Phosphate Reductase NAGPR). The precursor is cleaved in the mitochondria into two distinct enzymes (NAGK-DUF619 and NAGPR). Native molecular weights of these proteins indicate that the kinase is an octamer whereas the reductase is a dimer. This CD also includes some gamma-proteobacteria (Xanthomonas and Xylella) NAG kinases with an N-terminal NAGK (ArgB) domain (this CD) and a C-terminal DUF619 domain. The DUF619 domain is described as a putative distant homolog of the acetyltransferase ArgA predicted to function in NAG synthase association in fungi. Eukaryotic sequences have an N-terminal mitochondrial transit peptide. Members of this NAG kinase domain CD belong to the Amino Acid Kinase Superfamily (AAK)..
	gnl\|CDD\|162559	536	849	314	Gaps:41	97.98	346	39.82	12.68	1e-106	TIGR01850 argC N-acetyl-gamma-glutamyl-phosphate reductase common form. This model represents the more common of two related families of N-acetyl-gamma-glutamyl-phosphate reductase an enzyme catalyzing the third step or Arg biosynthesis from Glu. The two families differ by phylogeny similarity clustering and the gap architecture in a multiple sequence alignment. Bacterial members of this family tend to be found within Arg biosynthesis operons.
	gnl\|CDD\|30351	533	850	318	Gaps:40	98.57	349	38.08	12.50	9e-87	COG0002 ArgC Acetylglutamate semialdehyde dehydrogenase [Amino acid transport and metabolism].
	gnl\|CDD\|166961	536	850	315	Gaps:49	97.67	344	36.61	16.07	1e-81	PRK00436 argC N-acetyl-gamma-glutamyl-phosphate reductase Validated.
	gnl\|CDD\|167827	26	455	430	Gaps:50	95.48	398	40.26	21.05	9e-79	PRK04531 PRK04531 acetylglutamate kinase Provisional.
	gnl\|CDD\|147097	279	449	171	Gaps:3	100.00	168	49.40	19.64	2e-68	pfam04768 DUF619 Protein of unknown function (DUF619). This region of unknown function is found at the C-terminus of Neurospora crassa acetylglutamate synthase (amino-acid acetyltransferase EC: 2.3.1.1). It is also found C-terminal to the amino acid kinase region (pfam00696) in some fungal acetylglutamate kinase enzymes.
	gnl\|CDD\|162026	45	272	228	Gaps:5	100.00	231	42.42	18.18	1e-60	TIGR00761 argB acetylglutamate kinase. This model describes N-acetylglutamate kinases (ArgB) of many prokaryotes and the N-acetylglutamate kinase domains of multifunctional proteins from yeasts. This enzyme is the second step in the "acetylated" ornithine biosynthesis pathway. A related group of enzymes representing the first step of the pathway contain a homologous domain and are excluded from this model.
	gnl\|CDD\|30894	43	296	254	Gaps:10	99.62	265	33.71	20.45	1e-55	COG0548 ArgB Acetylglutamate kinase [Amino acid transport and metabolism].
	gnl\|CDD\|166609	510	848	339	Gaps:42	96.80	375	30.03	16.80	4e-45	PLN02968 PLN02968 N-acetyl-gamma-glutamyl-phosphate reductase.
	gnl\|CDD\|176265	333	432	100	Gaps:2	100.00	98	68.37	13.27	1e-42	cd04263 DUF619-NAGK-FABP DUF619 domain of N-acetylglutamate kinase (NAGK) of the fungal arginine-biosynthetic pathway. DUF619-NAGK-FABP: DUF619 domain of N-acetylglutamate kinase (NAGK) of the fungal arginine-biosynthetic pathway (FABP). The nuclear-encoded mitochondrial polyprotein precursor (ARG5 6) consists of an N-terminal NAGK (ArgB) domain a central DUF619 domain and a C-terminal reductase domain (ArgC N-Acetylglutamate Phosphate Reductase NAGPR). The precursor is cleaved into two distinct enzymes (NAGK-DUF619 and NAGPR) in the mitochondria. Native molecular weights of these proteins indicate that the kinase is an octamer whereas the reductase is a dimer. Arg5 6 catalyzes the second reaction of arginine biosynthesis the phosphorylation of the gamma-carboxyl group of NAG to produce N-acetylglutamylphosphate (NAGP) which is subsequently converted to ornithine in two more steps. It also binds and regulates the promoters of nuclear and mitochondrial genes and may possibly regulate precursor mRNA metabolism. The DUF619 domain function has yet to be characterized.
rpsblast_kog	gnl\|CDD\|39555	532	851	320	Gaps:4	94.12	340	54.69	14.69	1e-122	KOG4354 KOG4354 KOG4354 N-acetyl-gamma-glutamyl-phosphate reductase [Amino acid transport and metabolism].
rpsblast_kog	gnl\|CDD\|37647	23	435	413	Gaps:24	79.42	520	36.32	16.71	8e-84	KOG2436 KOG2436 KOG2436 Acetylglutamate kinase/acetylglutamate synthase [Amino acid transport and metabolism].

Other analysis

Functional Gbrowse

Ortholog