GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P146290.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_05875	1	404	404	Gaps:35	100.00	369	99.73	0.00	0.0	hypothetical protein
	cim:CIMG_01096	13	401	389	Gaps:18	92.79	430	49.12	18.30	1e-109	aspartate aminotransferase K00813 aspartate aminotransferase [EC:2.6.1.1]
	nfi:NFIA_048770	1	401	401	Gaps:12	99.27	412	44.74	20.05	1e-103	aspartate transaminase putative K00813 aspartate aminotransferase [EC:2.6.1.1]
	pcs:Pc22g23830	6	404	399	Gaps:12	97.84	416	45.45	19.41	1e-103	Pc22g23830 K00813 aspartate aminotransferase [EC:2.6.1.1]
	aor:AO090012000899	6	401	396	Gaps:10	97.83	415	47.04	16.01	1e-103	aspartate aminotransferase/Glutamic oxaloacetic transaminase AAT2/GOT1
	afv:AFLA_021460	6	401	396	Gaps:10	36.38	1116	47.04	16.01	1e-103	phosphate-repressible Na+/phosphate cotransporter Pho89 putative
	afm:AFUA_6G02490	1	401	401	Gaps:12	99.27	412	44.25	20.05	1e-101	aspartate transaminase (EC:2.6.1.1) K00813 aspartate aminotransferase [EC:2.6.1.1]
	ang:An12g07870	6	401	396	Gaps:15	98.07	415	44.72	19.90	1e-101	hypothetical protein K00813 aspartate aminotransferase [EC:2.6.1.1]
	nfi:NFIA_020570	6	401	396	Gaps:16	90.99	444	47.03	17.33	1e-101	aspartate aminotransferase putative K00813 aspartate aminotransferase [EC:2.6.1.1]
	afm:AFUA_1G04160	6	401	396	Gaps:16	90.99	444	46.53	17.33	1e-99	aspartate aminotransferase (EC:2.6.1.1) K00813 aspartate aminotransferase [EC:2.6.1.1]
blastp_uniprot_sprot	sp\|O42652\|AATC_SCHPO	4	397	394	Gaps:10	96.82	409	42.68	20.45	2e-91	Aspartate aminotransferase cytoplasmic OS Schizosaccharomyces pombe GN aat2 PE 2 SV 1
	sp\|P23542\|AATC_YEAST	6	401	396	Gaps:12	96.17	418	40.80	19.65	4e-86	Aspartate aminotransferase cytoplasmic OS Saccharomyces cerevisiae GN AAT2 PE 1 SV 3
	sp\|A5A6K8\|AATC_PANTR	4	394	391	Gaps:11	95.88	413	40.91	19.19	2e-83	Aspartate aminotransferase cytoplasmic OS Pan troglodytes GN GOT1 PE 2 SV 1
	sp\|P17174\|AATC_HUMAN	4	394	391	Gaps:11	95.88	413	40.91	19.19	2e-83	Aspartate aminotransferase cytoplasmic OS Homo sapiens GN GOT1 PE 1 SV 3
	sp\|Q5R691\|AATC_PONAB	4	394	391	Gaps:11	95.88	413	40.91	18.94	3e-83	Aspartate aminotransferase cytoplasmic OS Pongo abelii GN GOT1 PE 2 SV 1
	sp\|Q4R5L1\|AATC_MACFA	4	401	398	Gaps:11	97.58	413	40.20	19.35	6e-83	Aspartate aminotransferase cytoplasmic OS Macaca fascicularis GN GOT1 PE 2 SV 1
	sp\|P13221\|AATC_RAT	4	394	391	Gaps:11	95.88	413	40.40	19.19	3e-82	Aspartate aminotransferase cytoplasmic OS Rattus norvegicus GN Got1 PE 1 SV 3
	sp\|P00503\|AATC_PIG	4	394	391	Gaps:11	95.88	413	39.90	19.95	1e-81	Aspartate aminotransferase cytoplasmic OS Sus scrofa GN GOT1 PE 1 SV 3
	sp\|P33097\|AATC_BOVIN	4	394	391	Gaps:11	95.88	413	40.66	18.43	2e-81	Aspartate aminotransferase cytoplasmic OS Bos taurus GN GOT1 PE 1 SV 3
	sp\|P12344\|AATM_BOVIN	12	400	389	Gaps:9	90.23	430	42.01	18.30	2e-81	Aspartate aminotransferase mitochondrial OS Bos taurus GN GOT2 PE 1 SV 2
blastp_pdb	1yaa_D	6	401	396	Gaps:12	97.57	412	40.80	19.65	9e-87	mol:protein length:412 ASPARTATE AMINOTRANSFERASE
	1yaa_C	6	401	396	Gaps:12	97.57	412	40.80	19.65	9e-87	mol:protein length:412 ASPARTATE AMINOTRANSFERASE
	1yaa_B	6	401	396	Gaps:12	97.57	412	40.80	19.65	9e-87	mol:protein length:412 ASPARTATE AMINOTRANSFERASE
	1yaa_A	6	401	396	Gaps:12	97.57	412	40.80	19.65	9e-87	mol:protein length:412 ASPARTATE AMINOTRANSFERASE
	3ii0_D	11	394	384	Gaps:11	92.18	422	40.62	19.54	6e-82	mol:protein length:422 Aspartate aminotransferase cytoplasmic
	3ii0_C	11	394	384	Gaps:11	92.18	422	40.62	19.54	6e-82	mol:protein length:422 Aspartate aminotransferase cytoplasmic
	3ii0_B	11	394	384	Gaps:11	92.18	422	40.62	19.54	6e-82	mol:protein length:422 Aspartate aminotransferase cytoplasmic
	3ii0_A	11	394	384	Gaps:11	92.18	422	40.62	19.54	6e-82	mol:protein length:422 Aspartate aminotransferase cytoplasmic
	1ajs_B	4	394	391	Gaps:11	96.12	412	39.65	20.20	1e-81	mol:protein length:412 ASPARTATE AMINOTRANSFERASE
	1ajs_A	4	394	391	Gaps:11	96.12	412	39.65	20.20	1e-81	mol:protein length:412 ASPARTATE AMINOTRANSFERASE
rpsblast_cdd	gnl\|CDD\|173569	1	401	401	Gaps:9	99.01	404	43.50	19.00	1e-129	PTZ00376 PTZ00376 aspartate aminotransferase Provisional.
	gnl\|CDD\|169748	3	402	400	Gaps:9	100.00	397	41.56	18.14	1e-116	PRK09257 PRK09257 aromatic amino acid aminotransferase Provisional.
	gnl\|CDD\|31637	5	401	397	Gaps:9	99.49	396	42.13	19.04	1e-115	COG1448 TyrB Aspartate/tyrosine/aromatic aminotransferase [Amino acid transport and metabolism].
	gnl\|CDD\|166038	4	400	397	Gaps:8	92.94	425	38.99	21.77	1e-110	PLN02397 PLN02397 aspartate transaminase.
	gnl\|CDD\|143923	28	397	370	Gaps:47	100.00	351	27.07	16.24	5e-58	pfam00155 Aminotran_1_2 Aminotransferase class I and II.
	gnl\|CDD\|99734	31	391	361	Gaps:54	98.00	350	21.57	15.45	9e-33	cd00609 AAT_like Aspartate aminotransferase family. This family belongs to pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). Pyridoxal phosphate combines with an alpha-amino acid to form a compound called a Schiff base or aldimine intermediate which depending on the reaction is the substrate in four kinds of reactions (1) transamination (movement of amino groups) (2) racemization (redistribution of enantiomers) (3) decarboxylation (removing COOH groups) and (4) various side-chain reactions depending on the enzyme involved. Pyridoxal phosphate (PLP) dependent enzymes were previously classified into alpha beta and gamma classes based on the chemical characteristics (carbon atom involved) of the reaction they catalyzed. The availability of several structures allowed a comprehensive analysis of the evolutionary classification of PLP dependent enzymes and it was found that the functional classification did not always agree with the evolutionary history of these enzymes. The major groups in this CD corresponds to Aspartate aminotransferase a b and c Tyrosine Alanine Aromatic-amino-acid Glutamine phenylpyruvate 1-Aminocyclopropane-1-carboxylate synthase Histidinol-phosphate gene products of malY and cobC Valine-pyruvate aminotransferase and Rhizopine catabolism regulatory protein..
	gnl\|CDD\|30785	4	403	400	Gaps:62	98.73	393	20.62	14.43	2e-13	COG0436 COG0436 Aspartate/tyrosine/aromatic aminotransferase [Amino acid transport and metabolism].
	gnl\|CDD\|30428	99	404	306	Gaps:45	78.37	356	23.66	19.00	6e-11	COG0079 HisC Histidinol-phosphate/aromatic aminotransferase and cobyric acid decarboxylase [Amino acid transport and metabolism].
	gnl\|CDD\|169528	122	367	246	Gaps:21	63.64	385	23.67	20.82	1e-07	PRK08637 PRK08637 hypothetical protein Provisional.
	gnl\|CDD\|99742	98	265	168	Gaps:21	87.65	170	23.49	15.44	1e-07	cd01494 AAT_I Aspartate aminotransferase (AAT) superfamily (fold type I) of pyridoxal phosphate (PLP)-dependent enzymes. PLP combines with an alpha-amino acid to form a compound called a Schiff base or aldimine intermediate which depending on the reaction is the substrate in four kinds of reactions (1) transamination (movement of amino groups) (2) racemization (redistribution of enantiomers) (3) decarboxylation (removing COOH groups) and (4) various side-chain reactions depending on the enzyme involved. Pyridoxal phosphate (PLP) dependent enzymes were previously classified into alpha beta and gamma classes based on the chemical characteristics (carbon atom involved) of the reaction they catalyzed. The availability of several structures allowed a comprehensive analysis of the evolutionary classification of PLP dependent enzymes and it was found that the functional classification did not always agree with the evolutionary history of these enzymes. Structure and sequence analysis has revealed that the PLP dependent enzymes can be classified into four major groups of different evolutionary origin: aspartate aminotransferase superfamily (fold type I) tryptophan synthase beta superfamily (fold type II) alanine racemase superfamily (fold type III) and D-amino acid superfamily (fold type IV) and Glycogen phophorylase family (fold type V)..
rpsblast_kog	gnl\|CDD\|36626	5	401	397	Gaps:6	96.83	410	43.07	18.64	1e-111	KOG1412 KOG1412 KOG1412 Aspartate aminotransferase/Glutamic oxaloacetic transaminase AAT2/GOT1 [Amino acid transport and metabolism].
	gnl\|CDD\|36625	4	400	397	Gaps:8	92.51	427	42.28	22.03	4e-98	KOG1411 KOG1411 KOG1411 Aspartate aminotransferase/Glutamic oxaloacetic transaminase AAT1/GOT2 [Amino acid transport and metabolism].
	gnl\|CDD\|35478	46	376	331	Gaps:30	76.90	420	21.05	14.55	4e-07	KOG0257 KOG0257 KOG0257 Kynurenine aminotransferase glutamine transaminase K [Amino acid transport and metabolism].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	YCIA_533826	224615	225196	521	100.00	100.00	n/a	Botrytis cinerea HortResearch libraries
sim4_Bot_allest4_clctg	no results
sim4_Ss_allest	no results
blastx_uniprot_sprot	O42652	224669	225979	409	43.48	89.98	n/a	Aspartate aminotransferase cytoplasmic - Schizosaccharomyces pombe (Fission yeast)
	A5A6K8	224669	225979	413	41.56	93.22	n/a	Aspartate aminotransferase cytoplasmic - Pan troglodytes (Chimpanzee)
	P17174	224669	225979	413	41.56	93.22	n/a	Aspartate aminotransferase cytoplasmic - Homo sapiens (Human)
	Q5R691	224669	225979	413	41.56	93.22	n/a	Aspartate aminotransferase cytoplasmic - Pongo pygmaeus (Orangutan)
	Q4R5L1	224669	225979	413	41.56	93.22	n/a	Aspartate aminotransferase cytoplasmic - Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)
	P13221	224669	225979	413	41.04	93.22	n/a	Aspartate aminotransferase cytoplasmic - Rattus norvegicus (Rat)
	P00503	224669	225979	413	40.52	93.22	n/a	Aspartate aminotransferase cytoplasmic - Sus scrofa (Pig)
	P33097	224669	225979	413	41.04	93.22	n/a	Aspartate aminotransferase cytoplasmic - Bos taurus (Bovine)
	P05201	224669	225979	413	40.78	93.22	n/a	Aspartate aminotransferase cytoplasmic - Mus musculus (Mouse)
	P12344	224669	225979	430	41.95	88.14	n/a	Aspartate aminotransferase mitochondrial precursor - Bos taurus (Bovine)
blastx_uniprot_trembl	Q1E8L7	224684	225979	430	50.00	86.98	n/a	Aspartate aminotransferase - Coccidioides immitis
	A1DL69	224657	225979	412	45.01	94.90	n/a	Aspartate aminotransferase - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q4WCU8	224657	225979	412	44.50	94.90	n/a	Aspartate aminotransferase - Aspergillus fumigatus (Sartorya fumigata)
	A2R099	224672	225979	415	45.24	93.73	n/a	Aspartate aminotransferase - Aspergillus niger
	A1D4K6	224672	225979	444	47.55	87.16	n/a	Aspartate aminotransferase putative - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q0CGI9	224657	225979	418	44.67	94.26	n/a	Aspartate aminotransferase - Aspergillus terreus (strain NIH 2624)
	A2QTK1	224675	225979	413	49.73	90.56	n/a	Contig An09c0060 complete genome. - Aspergillus niger
	Q5B082	224669	225979	445	46.63	86.74	n/a	Putative uncharacterized protein - Emericella nidulans (Aspergillus nidulans)
	Q4X1K5	224669	225979	469	47.14	81.88	n/a	Aspartate transaminase putative - Aspergillus fumigatus (Sartorya fumigata)
	A1DL69	224657	225979	412	45.01	94.90	n/a	Aspartate aminotransferase - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q4WCU8	224657	225979	412	44.50	94.90	n/a	Aspartate aminotransferase - Aspergillus fumigatus (Sartorya fumigata)
	Q0CGI9	224657	225979	418	44.67	94.26	n/a	Aspartate aminotransferase - Aspergillus terreus (strain NIH 2624)
blastx_FNG30_prot	SNU08795.1	224660	225979	424	47.24	93.87	n/a	Stagonospora nodorum hypothetical protein translation
	CIMG_01096.2	224684	225979	430	50.00	86.98	n/a	Coccidioides immitis aspartate aminotransferase translation 431 aa
	FG11128.1	224657	225979	423	46.68	89.13	n/a	fusarium_graminearum hypothetical protein 424 aa
	gi:70984643:ref:XP_747828.1:	224657	225979	412	44.50	94.90	n/a	Aspergillus fumigatus Af293 aspartate transaminase
	FG03981.1	224961	225979	378	51.99	79.89	n/a	fusarium_graminearum hypothetical protein 379 aa
	AN6048	224669	225979	445	46.63	86.74	n/a	Aspergillus nidulans AN6048.3 conserved hypothetical protein translation 446 aa
	gi:70990632:ref:XP_750165.1:	224672	225979	444	47.03	87.16	n/a	Aspergillus fumigatus Af293 aspartate aminotransferase
	gi:71001234:ref:XP_755298.1:	224669	225979	469	47.14	81.88	n/a	Aspergillus fumigatus Af293 aspartate transaminase
	AN8709	224672	225979	416	44.36	93.75	n/a	Aspergillus nidulans AN8709.3 conserved hypothetical protein translation 417 aa
	AO090011000679	224675	225979	443	46.35	86.68	n/a	Aspergillus oryzae aspartate aminotransferase/Glutamic oxaloacetic transaminase AAT2/GOT1
	FG11128.1	224657	225979	423	46.68	89.13	n/a	fusarium_graminearum hypothetical protein 424 aa
	gi:70984643:ref:XP_747828.1:	224657	225979	412	44.50	94.90	n/a	Aspergillus fumigatus Af293 aspartate transaminase
sim4_B0510_genes	BC1G_05875.1	224657	226096	1110	99.82	100.00	n/a	n/a
sim4_SS_genes	no results
blastn_b0510_supercontig	B0510_supercontig_1.28	215508	229940	372624	99.66	n/a	n/a	n/a
blastn_SS_supercontig	no results
blat_oligo	botrytisP00148401	224680	224739	60	n/a	n/a	n/a	array_gene:BofuT4_P146290_1 . max_over_threshold:2.32 . min_over_threshold:0.35 . percent_hyb_over_threshold:28.57 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00148402	224794	224914	60	n/a	n/a	n/a	array_gene:BofuT4_P146290_1 . max_over_threshold:3.8 . min_over_threshold:0.3 . percent_hyb_over_threshold:35.71 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00148403	224972	225031	60	n/a	n/a	n/a	array_gene:BofuT4_P146290_1 . max_over_threshold:15.37 . min_over_threshold:0.42 . percent_hyb_over_threshold:42.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00148404	225092	225151	60	n/a	n/a	n/a	array_gene:BofuT4_P146290_1 . max_over_threshold:15.92 . min_over_threshold:0.29 . percent_hyb_over_threshold:42.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00148405	225218	225277	60	n/a	n/a	n/a	array_gene:BofuT4_P146290_1 . max_over_threshold:7.32 . min_over_threshold:0.34 . percent_hyb_over_threshold:39.28 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00148406	225344	225460	60	n/a	n/a	n/a	array_gene:BofuT4_P146290_1 . max_over_threshold:33.54 . min_over_threshold:0.41 . percent_hyb_over_threshold:42.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00148407	225574	225633	60	n/a	n/a	n/a	array_gene:BofuT4_P146290_1 . max_over_threshold:21.64 . min_over_threshold:1.32 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:1
	botrytisP00148408	225590	225649	60	n/a	n/a	n/a	array_gene:BofuT4_P146290_1 . max_over_threshold:18.81 . min_over_threshold:0.89 . percent_hyb_over_threshold:92.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:1
	botrytisP00148410	225810	225922	60	n/a	n/a	n/a	array_gene:BofuT4_P146290_1 . max_over_threshold:9.4 . min_over_threshold:0.36 . percent_hyb_over_threshold:42.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog