GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P138310.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	ssl:SS1G_09971	34	842	809	Gaps:33	96.85	826	79.88	3.50	0.0	hypothetical protein
	bfu:BC1G_07268	32	829	798	n/a	95.72	724	90.19	0.14	0.0	hypothetical protein
	fgr:FG06228.1	290	842	553	Gaps:23	72.36	738	65.73	13.30	0.0	hypothetical protein
	ncr:NCU08319	190	842	653	Gaps:41	78.97	780	60.23	13.64	0.0	developmental regulator flbA
	pan:PODANSg7195	296	842	547	Gaps:45	75.99	679	65.50	13.95	0.0	hypothetical protein
	cim:CIMG_07972	322	842	521	Gaps:23	73.30	693	60.63	14.76	1e-175	hypothetical protein
	ure:UREG_02258	199	842	644	Gaps:88	92.04	691	52.67	14.62	1e-172	developmental regulator flbA
	afv:AFLA_134030	305	842	538	Gaps:20	73.16	719	59.32	14.26	1e-172	developmental regulator FlbA
	aor:AO090026000532	305	842	538	Gaps:20	73.16	719	59.32	14.26	1e-172	hypothetical protein
	act:ACLA_070140	288	842	555	Gaps:31	72.48	734	58.65	14.10	1e-170	developmental regulator FlbA
blastp_uniprot_sprot	sp\|P38093\|FLBA_EMENI	296	842	547	Gaps:27	74.27	719	57.87	14.61	1e-170	Developmental regulator flbA OS Emericella nidulans GN flbA PE 2 SV 1
	sp\|Q09777\|RGS1_SCHPO	314	807	494	Gaps:49	94.59	481	29.89	20.88	1e-37	Regulator of G-protein signaling 1 OS Schizosaccharomyces pombe GN rgs1 PE 2 SV 1
	sp\|P11972\|SST2_YEAST	534	765	232	Gaps:56	38.11	698	28.57	18.80	3e-22	Protein SST2 OS Saccharomyces cerevisiae GN SST2 PE 1 SV 2
	sp\|P49802\|RGS7_HUMAN	668	816	149	Gaps:33	23.43	495	38.79	19.83	5e-09	Regulator of G-protein signaling 7 OS Homo sapiens GN RGS7 PE 1 SV 3
	sp\|O46470\|RGS7_BOVIN	668	816	149	Gaps:33	24.73	469	42.24	20.69	7e-09	Regulator of G-protein signaling 7 OS Bos taurus GN RGS7 PE 1 SV 1
	sp\|P49803\|RGS7_RAT	668	816	149	Gaps:33	24.32	477	42.24	20.69	8e-09	Regulator of G-protein signaling 7 OS Rattus norvegicus GN Rgs7 PE 2 SV 2
	sp\|Q8K443\|RGS13_MOUSE	666	817	152	Gaps:34	75.95	158	35.00	25.83	1e-08	Regulator of G-protein signaling 13 OS Mus musculus GN Rgs13 PE 1 SV 1
	sp\|O54829\|RGS7_MOUSE	668	816	149	Gaps:33	24.73	469	41.38	21.55	1e-08	Regulator of G-protein signaling 7 OS Mus musculus GN Rgs7 PE 1 SV 1
	sp\|Q2M5E4\|RGS21_HUMAN	665	817	153	Gaps:32	79.61	152	35.54	23.14	2e-07	Regulator of G-protein signaling 21 OS Homo sapiens GN RGS21 PE 2 SV 1
blastp_pdb	2a72_B	668	816	149	Gaps:33	79.45	146	38.79	19.83	7e-10	mol:protein length:146 Regulator of G-protein signalling 7
	2a72_A	668	816	149	Gaps:33	79.45	146	38.79	19.83	7e-10	mol:protein length:146 Regulator of G-protein signalling 7
	2d9j_A	668	815	148	Gaps:33	82.73	139	39.13	19.13	1e-09	mol:protein length:139 Regulator of G-protein signalling 7
	1zv4_X	663	811	149	Gaps:32	74.05	158	37.61	22.22	2e-07	mol:protein length:158 Regulator of G-protein signaling 17
rpsblast_cdd	gnl\|CDD\|144270	667	813	147	Gaps:31	99.15	117	42.24	16.38	5e-28	pfam00615 RGS Regulator of G protein signaling domain. RGS family members are GTPase-activating proteins for heterotrimeric G-protein alpha-subunits.
	gnl\|CDD\|128610	667	811	145	Gaps:30	97.46	118	40.00	18.26	3e-24	smart00315 RGS Regulator of G protein signalling domain. RGS family members are GTPase-activating proteins for heterotrimeric G-protein alpha-subunits.
	gnl\|CDD\|80333	530	629	100	Gaps:12	100.00	88	42.05	13.64	7e-17	cd04450 DEP_RGS7-like DEP (Dishevelled Egl-10 and Pleckstrin) domain found in RGS (regulator of G-protein signaling) proteins of the subfamily R7. This subgroup contains RGS7 RGS6 RGS9 and RGS11. They share a common domain architecture containing beside the RGS domain a DEP domain and a GGL (G-protein gamma subunit-like ) domain. RGS proteins are GTPase-activating (GAP) proteins of heterotrimeric G proteins by increasing the rate of GTP hydrolysis of the alpha subunit. The fungal homologs like yeast Sst2 share a related common domain architecture containing RGS and DEP domains. Sst2 has been identified as the principal regulator of mating pheromone signaling and recently the DEP domain of Sst2 has been shown to be necessary and sufficient to mediate receptor interaction..
	gnl\|CDD\|144265	541	629	89	Gaps:20	100.00	73	34.25	16.44	1e-11	pfam00610 DEP Domain found in Dishevelled Egl-10 and Pleckstrin (DEP). The DEP domain is responsible for mediating intracellular protein targeting and regulation of protein stability in the cell. The DEP domain is present in a number of signaling molecules including Regulator of G protein Signaling (RGS) proteins and has been implicated in membrane targeting. New findings in yeast however demonstrate a major role for a DEP domain in mediating the interaction of an RGS protein to the C-terminal tail of a GPCR thus placing RGS in close proximity with its substrate G protein alpha subunit.
	gnl\|CDD\|80317	530	628	99	Gaps:18	100.00	81	34.57	12.35	1e-08	cd04371 DEP DEP domain named after Dishevelled Egl-10 and Pleckstrin where this domain was first discovered. The function of this domain is still not clear but it is believed to be important for the membrane association of the signaling proteins in which it is present. New studies show that the DEP domain of Sst2 a yeast RGS protein is necessary and sufficient for receptor interaction..
rpsblast_kog	gnl\|CDD\|38796	666	819	154	Gaps:28	57.01	221	35.71	16.67	9e-10	KOG3589 KOG3589 KOG3589 G protein signaling regulators [Signal transduction mechanisms].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	PD0AGA8YB07CM1	207283	207659	377	99.73	100.00	n/a	BT4 mycelium - different stresses library
sim4_Bot_allest	PD0AGA17YL07CM1	206953	207500	496	99.40	100.00	n/a	BT4 mycelium - different stresses library
sim4_Bot_allest4_clctg	CL_bt4_SupSuperContig_90r_117_107r_1_bt4ctg_1926_006.Contig1_v5	206953	207659	655	99.39	100.00	n/a	all_lib_clone_nr:2
sim4_Ss_allest	no results
blastx_uniprot_sprot	P38093	204881	207283	719	53.70	99.72	n/a	Developmental regulator flbA - Emericella nidulans (Aspergillus nidulans)
blastx_uniprot_trembl	Q6B971	205613	207283	510	70.33	99.80	n/a	CPRGS-1 - Cryphonectria parasitica (Chesnut blight fungus) (Endothia parasitica)
	Q2H0L9	205154	207283	687	62.62	92.29	n/a	Putative uncharacterized protein - Chaetomium globosum (Soil fungus)
	Q2LD94	205097	207283	714	60.89	91.32	n/a	Regulator of G-protein signaling - Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea)
	Q7SA69	204887	207283	780	58.70	92.82	n/a	Developmental regulator flbA - Neurospora crassa
	A1EAA5	205613	207283	492	67.62	99.80	n/a	Regulator of G protein signaling - Beauveria bassiana
	Q1DNZ1	205154	207283	693	55.05	95.67	n/a	Putative uncharacterized protein - Coccidioides immitis
	A1DH35	204950	207283	712	52.89	99.58	n/a	Developmental regulator FlbA - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	A2QCD2	204947	207283	725	51.52	99.59	n/a	Function: flbA encodes an Emericella nidulans RGS - Aspergillus niger
blastx_FNG30_prot	SS1G_09971.1	204641	207111	826	85.77	95.28	n/a	sclerotinia_sclerotiorum hypothetical protein translation 827 aa
	jgi:Triree:32928	205613	207283	504	68.19	99.80	n/a	Trichoderma reesei
	CHG04677.1	205154	207283	687	62.62	92.29	n/a	Chaetomium globosum hypothetical protein translation 688 aa
	FG06228.1	205061	207283	738	61.48	91.46	n/a	fusarium_graminearum hypothetical protein 739 aa
	NCU08319.2	204887	207283	745	57.88	96.24	n/a	Neurospora crassa hypothetical protein translation 746 aa
	AN5893	204881	207283	719	53.70	99.72	n/a	Aspergillus nidulans AN5893.3 developmental regulator flbA translation 720 aa
	CIMG_07972.2	205154	207283	693	55.05	95.67	n/a	Coccidioides immitis hypothetical protein translation 694 aa
	gi:71001540:ref:XP_755451.1:	204950	207283	718	52.59	99.58	n/a	Aspergillus fumigatus Af293 developmental regulator FlbA
	AO090026000532	205567	207283	719	58.60	75.24	n/a	Aspergillus oryzae predicted protein
	SNU05180.1	205286	207214	670	50.34	88.36	n/a	Stagonospora nodorum hypothetical protein translation
	SS1G_09971.1	204641	207111	826	85.77	95.28	n/a	sclerotinia_sclerotiorum hypothetical protein translation 827 aa
sim4_B0510_genes	BC1G_07268.1	204641	207246	2175	99.86	100.00	n/a	n/a
sim4_B0510_genes	BC1G_07268.1	204641	207246	2175	99.86	100.00	n/a	n/a
sim4_SS_genes	SS1G_09971.1	204641	207286	2481	89.01	88.75	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.40	204597	204666	291568	100.00	n/a	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.40	204729	207317	291568	99.96	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.14	205704	207109	1192843	91.11	n/a	n/a	n/a
	SS_supercontig_1.14	205491	205680	1192843	90.00	n/a	n/a	n/a
	SS_supercontig_1.14	205173	205304	1192843	91.67	n/a	n/a	n/a
	SS_supercontig_1.14	207148	207223	1192843	92.11	n/a	n/a	n/a
	SS_supercontig_1.14	204966	205020	1192843	94.55	n/a	n/a	n/a
blat_oligo	botrytisP00142500	205961	206020	60	n/a	n/a	n/a	array_gene:BofuT4_P138310_1 . max_over_threshold:34.61 . min_over_threshold:2.06 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00142501	206156	206215	60	n/a	n/a	n/a	array_gene:BofuT4_P138310_1 . max_over_threshold:40.91 . min_over_threshold:2.68 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00142502	206384	206443	60	n/a	n/a	n/a	array_gene:BofuT4_P138310_1 . max_over_threshold:34.31 . min_over_threshold:3.78 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00142503	206469	206528	60	n/a	n/a	n/a	array_gene:BofuT4_P138310_1 . max_over_threshold:46.26 . min_over_threshold:4.02 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00142505	206541	206600	60	n/a	n/a	n/a	array_gene:BofuT4_P138310_1 . max_over_threshold:32.71 . min_over_threshold:2.71 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00142506	206669	206728	60	n/a	n/a	n/a	array_gene:BofuT4_P138310_1 . max_over_threshold:42.89 . min_over_threshold:4.68 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00142507	206929	206988	60	n/a	n/a	n/a	array_gene:BofuT4_P138310_1 . max_over_threshold:56.38 . min_over_threshold:5.29 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00142508	207062	207173	60	n/a	n/a	n/a	array_gene:BofuT4_P138310_1 . max_over_threshold:69.86 . min_over_threshold:5.82 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:1
	botrytisP00142509	207221	207280	60	n/a	n/a	n/a	array_gene:BofuT4_P138310_1 . max_over_threshold:28.34 . min_over_threshold:2.75 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog