GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P133600.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_11506	21	536	516	n/a	100.00	516	97.48	0.00	0.0	hypothetical protein
	ssl:SS1G_08287	21	536	516	n/a	100.00	516	85.27	6.59	0.0	hypothetical protein
	mgr:MGG_07089	20	533	514	Gaps:29	93.70	524	56.21	14.66	1e-157	MG07089.4 hypothetical protein
	fgr:FG04282.1	17	534	518	Gaps:31	94.43	575	52.12	13.63	1e-156	hypothetical protein
	ncr:NCU08022	25	533	509	Gaps:23	97.97	541	51.89	15.85	1e-151	similar to tRNA nucleotidyltransferase
	pan:PODANSg7239	20	533	514	Gaps:4	90.30	567	50.59	15.43	1e-144	hypothetical protein
	ang:An04g08680	26	533	508	Gaps:30	97.74	530	47.30	20.46	1e-128	hypothetical protein
	afm:AFUA_2G16660	19	533	515	Gaps:30	83.57	633	45.75	18.90	1e-123	tRNA nucleotidyltransferase (EC:2.7.7.25)
	cim:CIMG_09458	26	533	508	Gaps:28	83.84	625	45.80	19.66	1e-122	hypothetical protein
	nfi:NFIA_091970	19	533	515	Gaps:30	83.57	633	45.18	18.90	1e-121	poly(A) polymerase
blastp_uniprot_sprot	sp\|P21269\|CCA1_YEAST	26	518	493	Gaps:33	92.31	546	38.10	14.88	2e-76	tRNA nucleotidyltransferase mitochondrial OS Saccharomyces cerevisiae GN CCA1 PE 1 SV 1
	sp\|Q9P4S5\|CCA1_CANGA	14	518	505	Gaps:30	96.99	531	35.92	16.31	2e-75	tRNA nucleotidyltransferase mitochondrial OS Candida glabrata GN CCA1 PE 1 SV 2
	sp\|Q9UTQ0\|CCA1_SCHPO	21	515	495	Gaps:26	96.20	500	36.38	19.33	1e-71	Putative tRNA nucleotidyltransferase 1 OS Schizosaccharomyces pombe GN SPAC1093.04c PE 2 SV 2
	sp\|Q9Y7U9\|CCA2_SCHPO	26	533	508	Gaps:56	99.17	484	35.62	19.17	3e-70	Putative tRNA nucleotidyltransferase 2 OS Schizosaccharomyces pombe GN SPCC645.10 PE 2 SV 1
	sp\|B2G721\|CCA_LACRJ	53	287	235	Gaps:39	51.12	403	36.41	16.50	9e-18	CCA-adding enzyme OS Lactobacillus reuteri (strain JCM 1112) GN cca PE 3 SV 1
	sp\|A5VJK7\|CCA_LACRD	53	287	235	Gaps:39	51.12	403	36.41	16.50	9e-18	CCA-adding enzyme OS Lactobacillus reuteri (strain DSM 20016) GN cca PE 3 SV 1
	sp\|Q88W02\|CCA_LACPL	57	278	222	Gaps:37	47.42	407	36.79	19.69	9e-17	CCA-adding enzyme OS Lactobacillus plantarum GN cca PE 3 SV 1
	sp\|P44439\|PCNB_HAEIN	62	313	252	Gaps:26	51.77	452	28.63	23.08	1e-16	Probable poly(A) polymerase OS Haemophilus influenzae GN pcnB PE 3 SV 1
	sp\|Q1WU19\|CCA_LACS1	53	295	243	Gaps:59	53.47	404	37.04	16.67	1e-16	CCA-adding enzyme OS Lactobacillus salivarius subsp. salivarius (strain UCC118) GN cca PE 3 SV 1
	sp\|Q8Z9C3\|PCNB_SALTI	62	314	253	Gaps:28	50.54	465	28.94	22.13	2e-16	Poly(A) polymerase OS Salmonella typhi GN pcnB PE 3 SV 2
blastp_pdb	3h3a_B	61	302	242	Gaps:36	49.43	441	34.86	18.35	2e-18	mol:protein length:441 TRNA nucleotidyl transferase-related protein
	3h3a_A	61	302	242	Gaps:36	49.43	441	34.86	18.35	2e-18	mol:protein length:441 TRNA nucleotidyl transferase-related protein
	3h39_B	61	302	242	Gaps:36	49.43	441	34.86	18.35	2e-18	mol:protein length:441 TRNA nucleotidyl transferase-related protein
	3h39_A	61	302	242	Gaps:36	49.43	441	34.86	18.35	2e-18	mol:protein length:441 TRNA nucleotidyl transferase-related protein
	3h38_A	61	302	242	Gaps:36	49.43	441	34.86	18.35	2e-18	mol:protein length:441 TRNA nucleotidyl transferase-related protein
	3h37_B	61	302	242	Gaps:36	49.43	441	34.86	18.35	2e-18	mol:protein length:441 TRNA nucleotidyl transferase-related protein
	3h37_A	61	302	242	Gaps:36	49.43	441	34.86	18.35	2e-18	mol:protein length:441 TRNA nucleotidyl transferase-related protein
	1vfg_B	61	252	192	Gaps:28	43.59	390	35.88	22.35	4e-17	mol:protein length:390 poly A polymerase
	1vfg_A	61	252	192	Gaps:28	43.59	390	35.88	22.35	4e-17	mol:protein length:390 poly A polymerase
	1ou5_B	52	272	221	Gaps:37	44.20	448	33.33	20.71	2e-13	mol:protein length:448 tRNA CCA-adding enzyme
rpsblast_cdd	gnl\|CDD\|30962	57	327	271	Gaps:36	57.52	412	33.76	17.72	1e-42	COG0617 PcnB tRNA nucleotidyltransferase/poly(A) polymerase [Translation ribosomal structure and biogenesis].
	gnl\|CDD\|143388	32	200	169	Gaps:30	100.00	139	40.29	10.79	6e-33	cd05398 NT_ClassII-CCAase Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes. CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)] one nucleotide at a time onto the 3' end of tRNA in a template-independent reaction. This Class II group is comprised mainly of eubacterial and eukaryotic enzymes and includes Bacillus stearothermophilus CCAase Escherichia coli poly(A) polymerase I human mitochondrial CCAase and Saccharomyces cerevisiae CCAase (CCA1). CCA-adding enzymes have a single catalytic pocket which recognizes both ATP and CTP substrates. Included in this subgroup are CC- and A-adding enzymes from various ancient species of bacteria such as Aquifex aeolicus these enzymes collaborate to add CCA to tRNAs. This family belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily two carboxylates Dx[D/E] together with a third more distal carboxylate coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are fairly well conserved in this family. Escherichia coli CCAase is related to this group but has not been included in this alignment as this enzyme lacks the N-terminal helix conserved in the remainder of the NT superfamily.
	gnl\|CDD\|131739	57	300	244	Gaps:39	46.57	466	37.79	15.67	8e-27	TIGR02692 tRNA_CCA_actino tRNA adenylyltransferase. The enzyme tRNA adenylyltransferase also called tRNA-nucleotidyltransferase and CCA-adding enzyme can add or repair the required CCA triplet at the 3'-end of tRNA molecules. Genes encoding tRNA include the CCA tail in some but not all bacteria and this enzyme may be required for viability. Members of this family represent a distinct clade within the larger family pfam01743 (tRNA nucleotidyltransferase/poly(A) polymerase family protein). The example from Streptomyces coelicolor was shown to act as a CCA-adding enzyme and not as a poly(A) polymerase.
	gnl\|CDD\|110721	58	204	147	Gaps:27	100.00	126	37.30	14.29	8e-26	pfam01743 PolyA_pol Poly A polymerase head domain. This family includes nucleic acid independent RNA polymerases such as Poly(A) polymerase which adds the poly (A) tail to mRNA EC:2.7.7.19. This family also includes the tRNA nucleotidyltransferase that adds the CCA to the 3' of the tRNA EC:2.7.7.25.
	gnl\|CDD\|171963	147	286	140	Gaps:6	35.03	394	37.68	18.12	5e-22	PRK13299 PRK13299 tRNA CCA-pyrophosphorylase Provisional.
	gnl\|CDD\|130997	62	237	176	Gaps:25	37.32	410	33.33	26.80	9e-22	TIGR01942 pcnB poly(A) polymerase. This model describes the pcnB family of poly(A) polymerases (also known as plasmid copy number protein). These enzymes sequentially add adenosine nucleotides to the 3' end of RNAs targeting them for degradation by the cell. This was originally described for anti-sense RNAs but was later demonstrated for mRNAs as well. Members of this family are as yet limited to the gamma- and beta-proteobacteria with putative members in the Chlamydiacae and spirochetes. This family has homology to tRNA nucleotidyltransferase (cca).
	gnl\|CDD\|171209	62	237	176	Gaps:31	35.94	448	32.92	19.88	1e-17	PRK11623 pcnB poly(A) polymerase I Provisional.
	gnl\|CDD\|171962	164	273	110	Gaps:8	25.90	417	40.74	17.59	1e-14	PRK13298 PRK13298 tRNA CCA-pyrophosphorylase Provisional.
	gnl\|CDD\|170796	164	273	110	Gaps:18	26.34	410	41.67	18.52	3e-11	PRK10885 cca multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase Reviewed.
	gnl\|CDD\|106256	164	307	144	Gaps:12	38.89	360	30.00	22.86	2e-07	PRK13296 PRK13296 tRNA CCA-pyrophosphorylase Provisional.
rpsblast_kog	gnl\|CDD\|37370	36	299	264	Gaps:17	60.34	416	47.01	12.75	4e-62	KOG2159 KOG2159 KOG2159 tRNA nucleotidyltransferase/poly(A) polymerase [Translation ribosomal structure and biogenesis].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	YCOA_575394	281348	281610	263	97.72	100.00	n/a	Botrytis cinerea HortResearch libraries
sim4_Bot_allest	PD0AEA9YF16CM1	279864	280593	657	100.00	100.00	n/a	BT4 appressoria on teflon library
sim4_Bot_allest4_clctg	PD0AEA9YF16CM1	279864	280593	657	100.00	100.00	n/a	BT4 appressoria on teflon library
sim4_Ss_allest	G786P53RE8.T0	281038	281572	543	83.61	98.90	n/a	Ss ESTs grown as mycelia at neutral pH
blastx_uniprot_sprot	Q9P4S5	280844	281629	531	52.33	48.59	n/a	tRNA nucleotidyltransferase mitochondrial precursor - Candida glabrata (Yeast) (Torulopsis glabrata)
	P21269	280832	281593	546	54.58	45.97	n/a	tRNA nucleotidyltransferase mitochondrial precursor - Saccharomyces cerevisiae (Baker's yeast)
	Q9UTQ0	280279	281605	500	39.55	79.40	n/a	Putative tRNA nucleotidyltransferase 1 - Schizosaccharomyces pombe (Fission yeast)
	Q9Y7U9	280850	281593	484	45.31	50.62	n/a	Putative tRNA nucleotidyltransferase 2 - Schizosaccharomyces pombe (Fission yeast)
blastx_uniprot_trembl	Q2H0X0	279933	281614	528	53.45	96.02	n/a	Putative uncharacterized protein - Chaetomium globosum (Soil fungus)
	Q7SAL9	279936	281596	541	53.44	96.86	n/a	Putative uncharacterized protein - Neurospora crassa
	Q6MFS8	279936	281596	599	53.44	87.48	n/a	Related to tRNA nucleotidyltransferase - Neurospora crassa
	A2QJY3	279936	281587	530	48.25	96.98	n/a	Function: CCA1 carries out addition of CCA to 3'-end of tRNA without a template. - Aspergillus niger
	Q0CVM0	279936	281596	522	46.15	99.62	n/a	Putative uncharacterized protein - Aspergillus terreus (strain NIH 2624)
	Q1DJQ5	279936	281716	625	44.31	92.80	n/a	Putative uncharacterized protein - Coccidioides immitis
	Q4WZK0	279936	281695	633	44.95	87.52	n/a	TRNA nucleotidyltransferase - Aspergillus fumigatus (Sartorya fumigata)
	A1DIN0	279936	281695	633	44.22	87.52	n/a	Poly(A) polymerase - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
blastx_FNG30_prot	SS1G_08287.1	279927	281608	516	87.40	100.00	n/a	sclerotinia_sclerotiorum hypothetical protein translation 517 aa
	jgi:Triree:33452	279936	281614	545	55.67	92.29	n/a	Trichoderma reesei
	FG04282.1	279933	281608	575	55.88	88.70	n/a	fusarium_graminearum hypothetical protein 576 aa
	CHG04576.1	279933	281614	528	53.45	96.02	n/a	Chaetomium globosum hypothetical protein translation 529 aa
	NCU08022.2	279936	281596	599	53.44	87.48	n/a	Neurospora crassa hypothetical protein translation 600 aa
	AO090102000157	279927	281593	567	46.63	91.53	n/a	Aspergillus oryzae tRNA nucleotidyltransferase/polyA polymerase
	AN10924	279936	281617	605	45.19	84.13	n/a	Aspergillus nidulans AN10924.3 conserved hypothetical protein translation 606 aa
	MGG_07089.5	279936	281611	524	58.40	90.84	n/a	magnaporthe_grisea hypothetical protein translation 525 aa
	CIMG_09458.2	279936	281716	625	44.31	92.80	n/a	Coccidioides immitis hypothetical protein translation 626 aa
	gi:71002644:ref:XP_756003.1:	279936	281695	651	45.16	85.71	n/a	Aspergillus fumigatus Af293 tRNA nucleotidyltransferase
sim4_B0510_genes	BC1G_11506.1	279924	281608	1551	100.00	100.00	n/a	n/a
sim4_B0510_genes	BC1G_11506.1	279924	281608	1551	100.00	100.00	n/a	n/a
sim4_SS_genes	SS1G_08287.1	279924	281587	1551	84.96	87.43	n/a	n/a
sim4_SS_genes	SS1G_08287.1	279924	281587	1551	84.96	87.43	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.81	278333	285331	181037	100.00	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.11	280249	280816	1419214	83.45	n/a	n/a	n/a
	SS_supercontig_1.11	279928	280133	1419214	81.07	n/a	n/a	n/a
	SS_supercontig_1.11	280863	281781	1419214	83.57	n/a	n/a	n/a
blat_oligo	botrytisP00138800	281442	281501	60	n/a	n/a	n/a	array_gene:BofuT4_P133600_1 . max_over_threshold:12.95 . min_over_threshold:1.85 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:1
	botrytisP00138801	281256	281315	60	n/a	n/a	n/a	array_gene:BofuT4_P133600_1 . max_over_threshold:25.31 . min_over_threshold:3.18 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00138802	281118	281177	60	n/a	n/a	n/a	array_gene:BofuT4_P133600_1 . max_over_threshold:30.98 . min_over_threshold:5.81 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00138803	280996	281055	60	n/a	n/a	n/a	array_gene:BofuT4_P133600_1 . max_over_threshold:40.36 . min_over_threshold:10.41 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00138804	280897	280956	60	n/a	n/a	n/a	array_gene:BofuT4_P133600_1 . max_over_threshold:34.92 . min_over_threshold:5.44 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00138805	280574	280633	60	n/a	n/a	n/a	array_gene:BofuT4_P133600_1 . max_over_threshold:17.23 . min_over_threshold:2.43 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00138807	280337	280396	60	n/a	n/a	n/a	array_gene:BofuT4_P133600_1 . max_over_threshold:37.33 . min_over_threshold:6.83 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00138808	280149	280281	60	n/a	n/a	n/a	array_gene:BofuT4_P133600_1 . max_over_threshold:32.68 . min_over_threshold:5.21 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00138809	280016	280075	60	n/a	n/a	n/a	array_gene:BofuT4_P133600_1 . max_over_threshold:19.53 . min_over_threshold:2.2 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog