GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P125090.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_06035	1	516	516	n/a	100.00	516	93.22	0.19	0.0	hypothetical protein
	ssl:SS1G_02334	1	516	516	Gaps:18	100.00	504	79.37	5.36	0.0	hypothetical protein
	nfi:NFIA_057300	1	515	515	Gaps:9	98.11	530	59.42	9.81	1e-178	glycosyl hydrolase family 7 protein
	afm:AFUA_6G11610	1	515	515	Gaps:11	98.12	532	58.81	9.58	1e-176	1 4-beta-D-glucan-cellobiohydrolyase (EC:3.2.1.-) K01238 [EC:3.2.1.-]
	pcs:Pc20g01970	20	515	496	Gaps:9	94.71	529	60.28	9.58	1e-171	Pc20g01970
	ssl:SS1G_04945	5	452	448	Gaps:9	74.87	589	66.21	10.43	1e-168	hypothetical protein
	bfu:BC1G_14702	5	452	448	Gaps:9	73.62	599	66.21	9.52	1e-168	hypothetical protein
	ang:An01g11660	1	515	515	Gaps:20	99.07	536	56.50	11.11	1e-167	cbhB 1 4-beta-D-glucan cellobiohydrolase B precursor cbhB-Aspergillus niger (EC:3.2.1.91) K01225 cellulose 1 4-beta-cellobiosidase [EC:3.2.1.91]
	ssl:SS1G_09020	1	452	452	Gaps:11	99.78	450	67.04	8.46	1e-166	hypothetical protein
	afm:AFUA_6G07070	1	454	454	Gaps:10	100.00	452	63.27	11.28	1e-166	cellobiohydrolase celD
blastp_uniprot_sprot	sp\|A1DNL0\|CBHB_NEOFI	1	515	515	Gaps:9	98.11	530	59.42	9.81	1e-179	Probable 1 4-beta-D-glucan cellobiohydrolase B OS Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) GN cbhB PE 3 SV 1
	sp\|Q4WM08\|CBHB_ASPFU	1	515	515	Gaps:11	98.12	532	58.81	9.58	1e-177	Probable 1 4-beta-D-glucan cellobiohydrolase B OS Aspergillus fumigatus GN cbhB PE 2 SV 1
	sp\|B0Y8K2\|CBHB_ASPFC	1	515	515	Gaps:11	98.12	532	58.81	9.58	1e-177	Probable 1 4-beta-D-glucan cellobiohydrolase B OS Aspergillus fumigatus (strain CEA10 / CBS 144.89 / FGSC A1163) GN cbhB PE 3 SV 1
	sp\|P13860\|GUX1_PHACH	5	515	511	Gaps:20	98.64	516	61.49	11.20	1e-175	Exoglucanase 1 OS Phanerochaete chrysosporium GN CBH1 PE 2 SV 1
	sp\|Q0CMT2\|CBHB_ASPTN	16	515	500	Gaps:23	95.93	541	58.77	10.40	1e-172	Probable 1 4-beta-D-glucan cellobiohydrolase B OS Aspergillus terreus (strain NIH 2624 / FGSC A1156) GN cbhB PE 3 SV 1
	sp\|Q9UVS8\|CBHB_ASPNG	1	515	515	Gaps:20	99.07	536	56.69	10.92	1e-168	1 4-beta-D-glucan cellobiohydrolase B OS Aspergillus niger GN cbhB PE 2 SV 1
	sp\|A2QAI7\|CBHB_ASPNC	1	515	515	Gaps:20	99.07	536	56.50	11.11	1e-168	Probable 1 4-beta-D-glucan cellobiohydrolase B OS Aspergillus niger (strain CBS 513.88 / FGSC A1513) GN cbhB PE 3 SV 1
	sp\|Q4WNA2\|CBHA_ASPFU	1	454	454	Gaps:10	100.00	452	63.27	11.28	1e-167	Probable 1 4-beta-D-glucan cellobiohydrolase A OS Aspergillus fumigatus GN cbhA PE 3 SV 1
	sp\|B0Y793\|CBHA_ASPFC	1	454	454	Gaps:10	100.00	452	63.27	11.28	1e-167	Probable 1 4-beta-D-glucan cellobiohydrolase A OS Aspergillus fumigatus (strain CEA10 / CBS 144.89 / FGSC A1163) GN cbhA PE 3 SV 1
	sp\|A1CU44\|CBHB_ASPCL	1	515	515	Gaps:18	98.14	539	55.77	10.96	1e-167	Probable 1 4-beta-D-glucan cellobiohydrolase B OS Aspergillus clavatus GN cbhB PE 3 SV 1
blastp_pdb	1q9h_A	18	455	438	Gaps:9	100.00	437	63.84	11.44	1e-166	mol:protein length:437 cellobiohydrolase I catalytic domain
	1z3w_A	18	455	438	Gaps:12	99.77	431	65.81	11.40	1e-165	mol:protein length:431 cellulase
	1z3v_A	18	455	438	Gaps:12	99.77	431	65.81	11.40	1e-165	mol:protein length:431 cellulase
	1z3t_A	18	455	438	Gaps:12	99.77	431	65.81	11.40	1e-165	mol:protein length:431 cellulase
	1h46_X	18	455	438	Gaps:12	99.77	431	65.81	11.40	1e-165	mol:protein length:431 EXOGLUCANASE I
	1gpi_A	18	455	438	Gaps:12	99.77	431	65.81	11.40	1e-165	mol:protein length:431 EXOGLUCANASE I
	1q2b_A	18	456	439	Gaps:17	100.00	434	57.83	11.29	1e-141	mol:protein length:434 EXOCELLOBIOHYDROLASE I
	2v3r_A	18	456	439	Gaps:17	100.00	434	57.83	11.06	1e-141	mol:protein length:434 EXOGLUCANASE 1
	2v3i_A	18	456	439	Gaps:17	100.00	434	57.83	11.06	1e-141	mol:protein length:434 EXOGLUCANASE 1
	1dy4_A	18	456	439	Gaps:17	100.00	434	57.83	11.06	1e-141	mol:protein length:434 CELLOBIOHYDROLASE I
rpsblast_cdd	gnl\|CDD\|144436	19	454	436	Gaps:8	100.00	432	71.30	9.49	0.0	pfam00840 Glyco_hydro_7 Glycosyl hydrolase family 7.
	gnl\|CDD\|153432	26	446	421	Gaps:40	99.74	386	65.97	11.95	1e-164	cd07999 GH7_CBH_EG Glycosyl hydrolase family 7. Glycosyl hydrolase family 7 contains eukaryotic endoglucanases (EGs) and cellobiohydrolases (CBHs) that hydrolyze glycosidic bonds using a double-displacement mechanism. This leads to a net retention of the conformation at the anomeric carbon. Both enzymes work synergistically in the degradation of cellulose which is the main component of plant cell wall and is composed of beta-1 4 linked glycosyl units. EG cleaves the beta-1 4 linkages of cellulose and CBH cleaves off cellobiose disaccharide units from the reducing end of the chain. In general the O-glycosyl hydrolases are a widespread group of enzymes that hydrolyze the glycosidic bond between two or more carbohydrates or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycoside hydrolase family 7.
	gnl\|CDD\|128532	483	516	34	n/a	100.00	34	61.76	5.88	1e-08	smart00236 fCBD Fungal-type cellulose-binding domain. Small four-cysteine cellulose-binding domain of fungi.
	gnl\|CDD\|144364	484	512	29	n/a	100.00	29	55.17	10.34	3e-08	pfam00734 CBM_1 Fungal cellulose binding domain.
rpsblast_kog	no results

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	YCMA_556684	267672	268331	605	99.50	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCOA_562799	267672	268211	539	99.44	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCNA_551514	267675	267974	300	99.67	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCMA_574692	267929	268649	607	99.51	100.00	n/a	Botrytis cinerea HortResearch libraries
	B2BC_045_113_A12	268317	268736	529	98.63	69.00	n/a	B0510 Spores suspension on bean library
	B2BC_045_112_H11	268317	268735	546	98.90	66.48	n/a	B0510 Spores suspension on bean library
	B2BC_045_107_C09	267703	268732	412	98.79	100.00	n/a	B0510 Spores suspension on bean library
	B2BC_045_117_G02	268924	269259	408	98.52	82.60	n/a	B0510 Spores suspension on bean library
	B2BC_045_115_E11	268924	269258	355	97.92	94.65	n/a	B0510 Spores suspension on bean library
	B2BC_045_112_E10	268930	269253	324	99.38	100.00	n/a	B0510 Spores suspension on bean library
	B2BC_045_122_G06	268934	269256	323	99.38	100.00	n/a	B0510 Spores suspension on bean library
	B2BC_045_104_A08	268934	269259	344	98.48	95.35	n/a	B0510 Spores suspension on bean library
	B2BC_045_113_A10	268317	268684	476	98.72	65.55	n/a	B0510 Spores suspension on bean library
	B5BC_116_298_C02	267775	268638	750	99.60	100.00	n/a	B0510 In planta material
	B5BC_116_287_C10	267782	268751	856	99.42	100.00	n/a	B0510 In planta material
	B5BC_116_295_F10	268862	269542	707	98.53	96.46	n/a	B0510 In planta material
	B5BC_116_288_C08	269318	269549	234	97.01	100.00	n/a	B0510 In planta material
sim4_Bot_allest4_clctg	CL_bt4_SupSuperContig_36_28_1_bt4ctg_1735_005.Contig1_v5	267775	268732	844	99.64	100.00	n/a	all_lib_clone_nr:3
sim4_Bot_allest4_clctg	CL_bt4_SupSuperContig_36_28_1_bt4ctg_1735_006.Contig1_v5	268924	269542	648	97.74	95.68	n/a	all_lib_clone_nr:6
sim4_Ss_allest	G865P313RL24.T0	267643	268211	607	84.86	93.57	n/a	Ss ESTs in planta set from infected Brassica napus
	G865P313FI19.T0	267647	268434	684	85.21	98.83	n/a	Ss ESTs in planta set from infected Brassica napus
	G865P35RJ7.T0	267643	268467	723	85.09	98.34	n/a	Ss ESTs in planta set from infected Brassica napus
	G865P313RI19.T0	268940	269527	597	83.80	66.16	n/a	Ss ESTs in planta set from infected Brassica napus
	G865P313FL24.T0	268955	269526	611	81.90	55.16	n/a	Ss ESTs in planta set from infected Brassica napus
blastx_uniprot_sprot	P13860	267683	269329	516	64.83	98.64	n/a	Exoglucanase 1 precursor - Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
	Q7SA23	267671	269329	521	61.73	99.81	n/a	Exoglucanase 1 precursor - Neurospora crassa
	O59843	267674	269329	540	60.79	98.70	n/a	Exoglucanase 1 precursor - Aspergillus aculeatus
	P15828	267677	269329	525	60.65	99.24	n/a	Exoglucanase 1 precursor - Humicola grisea var. thermoidea
	Q92400	267683	269329	506	59.20	98.81	n/a	Exoglucanase precursor - Agaricus bisporus (Common mushroom)
	P38676	267671	269329	516	62.14	99.81	n/a	Exoglucanase 1 precursor - Neurospora crassa
	P46238	267683	269332	514	59.22	99.22	n/a	Putative exoglucanase type C precursor - Fusarium oxysporum
	Q9P8P3	267671	269329	505	60.12	99.80	n/a	Exoglucanase 1 precursor - Trichoderma harzianum (Hypocrea lixii)
	P19355	267671	269329	514	58.67	99.81	n/a	Exoglucanase 1 precursor - Trichoderma viride
	Q7SA23	267671	269329	521	61.73	99.81	n/a	Exoglucanase 1 precursor - Neurospora crassa
	Q06886	267665	269329	537	59.81	98.70	n/a	Exoglucanase 1 precursor - Penicillium janthinellum (Penicillium vitale)
	P38676	267671	269329	516	62.14	99.81	n/a	Exoglucanase 1 precursor - Neurospora crassa
	Q9P8P3	267671	269329	505	60.12	99.80	n/a	Exoglucanase 1 precursor - Trichoderma harzianum (Hypocrea lixii)
	P19355	267671	269329	514	58.67	99.81	n/a	Exoglucanase 1 precursor - Trichoderma viride
blastx_uniprot_trembl	Q01763	267674	269329	504	56.11	99.01	n/a	Cellulase - Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
	A4UWT3	267671	269140	446	57.34	99.33	n/a	Putative glycosyl hydrolase family7 - uncultured symbiotic protist of Hodotermopsis sjoestedti
	Q9Y895	267671	269329	536	55.00	93.28	n/a	CBHI - Volvariella volvacea
	A4UX58	267698	269140	447	54.50	99.33	n/a	Putative glycosyl hydrolase family7 - uncultured symbiotic protist of Cryptocercus punctulatus
	Q9Y723	267683	269329	523	65.70	98.66	n/a	Exocellulase precursor - Irpex lacteus (Milk-white toothed polypore) (Polyporus tulipiferae)
	Q75NB5	267683	269329	521	64.98	98.66	n/a	Cellobiohydrolase - Irpex lacteus (Milk-white toothed polypore) (Polyporus tulipiferae)
	A4UWT3	267671	269140	446	57.34	99.33	n/a	Putative glycosyl hydrolase family7 - uncultured symbiotic protist of Hodotermopsis sjoestedti
	Q9Y895	267671	269329	536	55.00	93.28	n/a	CBHI - Volvariella volvacea
	A1DNL0	267665	269329	530	63.41	98.49	n/a	Glycosyl hydrolase family 7 protein - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q4WM08	267665	269329	532	62.60	98.50	n/a	1 4-beta-D-glucan-cellobiohydrolyase putative - Aspergillus fumigatus (Sartorya fumigata)
blastx_FNG30_prot	SS1G_02334.1	267671	269332	504	84.72	100.00	n/a	sclerotinia_sclerotiorum hypothetical protein translation 505 aa
	jgi:Phchr1:137042:e_gww2.14.10	267674	269329	504	56.11	99.01	n/a	Phanerochaete chrysosporium
	jgi:Phchr1:127029:e_gwh2.14.89	267683	269329	516	65.03	98.64	n/a	Phanerochaete chrysosporium
	NCU07340.2	267671	269329	521	61.73	99.81	n/a	Neurospora crassa GUX1_NEUCR Exoglucanase 1 precursor Exocellobiohydrolase 1 14-beta-cellobiohydrolase translation 522 aa
	jgi:Phchr1:137216:e_gww2.3.257	267677	269329	511	64.23	99.02	n/a	Phanerochaete chrysosporium
	jgi:Phchr1:129310:e_gwh2.2.346	267683	269329	512	65.54	98.63	n/a	Phanerochaete chrysosporium
	jgi:Phchr1:129072:e_gwh2.2.348	267683	269329	512	65.54	98.63	n/a	Phanerochaete chrysosporium
	SS1G_02334.1	267671	269332	504	84.72	100.00	n/a	sclerotinia_sclerotiorum hypothetical protein translation 505 aa
	SNU11043.1	267665	269140	455	56.85	97.80	n/a	Stagonospora nodorum hypothetical protein translation
	gi:70992391:ref:XP_751044.1:	267665	269329	532	62.60	98.50	n/a	Aspergillus fumigatus Af293 14-beta-D-glucan-cellobiohydrolyase
	NCU07340.2	267671	269329	521	61.73	99.81	n/a	Neurospora crassa GUX1_NEUCR Exoglucanase 1 precursor Exocellobiohydrolase 1 14-beta-cellobiohydrolase translation 522 aa
	jgi:Phchr1:137372:e_gww2.3.232	267683	269350	551	65.02	91.83	n/a	Phanerochaete chrysosporium
sim4_B0510_genes	BC1G_06035.1	267671	269335	1551	99.61	100.00	n/a	n/a
sim4_B0510_genes	BC1G_14702.1	267816	268879	1800	83.24	20.89	n/a	n/a
sim4_SS_genes	SS1G_02334.1	267798	269335	1515	86.45	77.43	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.155	268269	268333	84521	90.77	n/a	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.30	266367	273413	372050	98.78	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.3	267759	268213	2631477	86.37	n/a	n/a	n/a
	SS_supercontig_1.3	268393	268767	2631477	84.53	n/a	n/a	n/a
	SS_supercontig_1.3	268814	269140	2631477	85.63	n/a	n/a	n/a
	SS_supercontig_1.3	268263	268333	2631477	94.37	n/a	n/a	n/a
blat_oligo	botrytisP00132334	267755	267814	60	n/a	n/a	n/a	array_gene:BofuT4_P125090_1 . max_over_threshold:197.69 . min_over_threshold:0.5 . percent_hyb_over_threshold:92.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00132335	268028	268087	60	n/a	n/a	n/a	array_gene:BofuT4_P125090_1 . max_over_threshold:228.96 . min_over_threshold:0.98 . percent_hyb_over_threshold:96.42 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00132336	268494	268553	60	n/a	n/a	n/a	array_gene:BofuT4_P125090_1 . max_over_threshold:252.22 . min_over_threshold:0.89 . percent_hyb_over_threshold:96.42 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00132337	268598	268657	60	n/a	n/a	n/a	array_gene:BofuT4_P125090_1 . max_over_threshold:227.27 . min_over_threshold:0.93 . percent_hyb_over_threshold:96.42 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00132338	268851	268910	60	n/a	n/a	n/a	array_gene:BofuT4_P125090_1 . max_over_threshold:147.01 . min_over_threshold:0.5 . percent_hyb_over_threshold:67.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00132339	268942	269001	60	n/a	n/a	n/a	array_gene:BofuT4_P125090_1 . max_over_threshold:203.73 . min_over_threshold:0.59 . percent_hyb_over_threshold:78.57 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00132340	269026	269085	60	n/a	n/a	n/a	array_gene:BofuT4_P125090_1 . max_over_threshold:215.45 . min_over_threshold:0.62 . percent_hyb_over_threshold:85.71 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00132342	269143	269202	60	n/a	n/a	n/a	array_gene:BofuT4_P125090_1 . max_over_threshold:208.37 . min_over_threshold:0.61 . percent_hyb_over_threshold:92.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00132343	269272	269331	60	n/a	n/a	n/a	array_gene:BofuT4_P125090_1 . max_over_threshold:134.67 . min_over_threshold:0.42 . percent_hyb_over_threshold:75 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog