GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P117340.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_02862	24	459	436	n/a	94.99	459	100.00	0.00	0.0	similar to cystathionine beta-lyase K01760 cystathionine beta-lyase [EC:4.4.1.8]
	ssl:SS1G_09924	24	459	436	n/a	94.99	459	97.25	1.15	0.0	similar to cystathionine beta-lyase K01760 cystathionine beta-lyase [EC:4.4.1.8]
	ncr:NCU07987	24	437	414	n/a	90.59	457	86.47	6.28	0.0	cystathionine beta-lyase K01760 cystathionine beta-lyase [EC:4.4.1.8]
	mgr:MGG_07074	27	449	423	Gaps:2	93.61	454	84.94	7.06	0.0	MG07074.4 similar to cystathionine beta-lyase K01760 cystathionine beta-lyase [EC:4.4.1.8]
	pan:PODANSg7402	24	440	417	Gaps:1	94.78	441	85.41	6.94	0.0	hypothetical protein K01760 cystathionine beta-lyase [EC:4.4.1.8]
	fgr:FG01932.1	30	449	420	Gaps:2	96.79	436	83.41	7.11	0.0	similar to AF401237_1 cystathionine beta-lyase K01760 cystathionine beta-lyase [EC:4.4.1.8]
	cim:CIMG_08682	24	445	422	n/a	92.14	458	81.04	8.53	0.0	similar to cystathionine beta-lyase
	afm:AFUA_4G03950	24	437	414	n/a	93.45	443	80.43	10.14	0.0	cystathionine beta-lyase MetG (EC:4.4.1.8) K01760 cystathionine beta-lyase [EC:4.4.1.8]
	nfi:NFIA_029440	24	437	414	n/a	93.45	443	80.19	10.39	0.0	cystathionine beta-lyase MetG K01760 cystathionine beta-lyase [EC:4.4.1.8]
	ure:UREG_02666	24	440	417	n/a	91.65	455	81.29	8.87	0.0	cystathionine beta-lyase
blastp_uniprot_sprot	sp\|O94350\|CBL_SCHPO	48	425	378	Gaps:2	97.44	390	52.89	15.79	1e-110	Cystathionine beta-lyase OS Schizosaccharomyces pombe GN str3 PE 2 SV 4
	sp\|P53780\|METC_ARATH	50	422	373	Gaps:5	80.60	464	51.34	19.25	1e-105	Cystathionine beta-lyase chloroplastic OS Arabidopsis thaliana GN At3g57050 PE 1 SV 1
	sp\|P53101\|STR3_YEAST	62	444	383	Gaps:29	88.60	465	49.27	14.81	1e-102	Cystathionine beta-lyase OS Saccharomyces cerevisiae GN STR3 PE 1 SV 1
	sp\|Q55DV9\|CGL_DICDI	41	422	382	Gaps:11	98.97	387	46.48	15.67	7e-83	Cystathionine gamma-lyase OS Dictyostelium discoideum GN cysA PE 1 SV 1
	sp\|A2RM21\|METC_LACLM	65	422	358	Gaps:4	94.21	380	43.02	16.76	4e-78	Cystathionine beta-lyase OS Lactococcus lactis subsp. cremoris (strain MG1363) GN metC PE 1 SV 1
	sp\|P0A4K2\|METC_LACLA	65	422	358	Gaps:4	94.21	380	43.02	16.76	4e-78	Cystathionine beta-lyase OS Lactococcus lactis subsp. lactis GN metC PE 3 SV 1
	sp\|P56069\|METB_HELPY	65	426	362	Gaps:4	95.26	380	42.27	17.96	5e-78	Cystathionine gamma-synthase OS Helicobacter pylori GN metB PE 3 SV 1
	sp\|O05394\|MCCB_BACSU	62	422	361	Gaps:4	95.25	379	41.83	19.94	1e-76	Cystathionine gamma-lyase OS Bacillus subtilis GN mccB PE 1 SV 1
	sp\|P0C2T9\|METC_LACLC	65	422	358	Gaps:4	94.21	380	42.74	16.76	2e-75	Cystathionine beta-lyase OS Lactococcus lactis subsp. cremoris GN metC PE 1 SV 1
	sp\|O31632\|METC_BACSU	42	430	389	Gaps:9	99.49	390	39.95	18.30	6e-75	Cystathionine beta-lyase metC OS Bacillus subtilis GN metC PE 1 SV 1
blastp_pdb	1ibj_C	50	422	373	Gaps:5	80.60	464	51.34	19.25	1e-105	mol:protein length:464 CYSTATHIONINE BETA-LYASE
	1ibj_A	50	422	373	Gaps:5	80.60	464	51.34	19.25	1e-105	mol:protein length:464 CYSTATHIONINE BETA-LYASE
	3nnp_B	44	424	381	Gaps:10	96.75	400	42.38	15.76	4e-72	mol:protein length:400 Cystathionine gamma-lyase-like protein
	3nnp_A	44	424	381	Gaps:10	96.75	400	42.38	15.76	4e-72	mol:protein length:400 Cystathionine gamma-lyase-like protein
	3e6g_D	44	424	381	Gaps:10	96.75	400	42.38	15.76	4e-72	mol:protein length:400 Cystathionine gamma-lyase-like protein
	3e6g_C	44	424	381	Gaps:10	96.75	400	42.38	15.76	4e-72	mol:protein length:400 Cystathionine gamma-lyase-like protein
	3e6g_B	44	424	381	Gaps:10	96.75	400	42.38	15.76	4e-72	mol:protein length:400 Cystathionine gamma-lyase-like protein
	3e6g_A	44	424	381	Gaps:10	96.75	400	42.38	15.76	4e-72	mol:protein length:400 Cystathionine gamma-lyase-like protein
	2rfv_A	47	425	379	Gaps:17	98.49	398	38.27	18.62	4e-68	mol:protein length:398 Methionine gamma-lyase
	3mkj_A	47	425	379	Gaps:17	98.49	398	38.27	18.62	4e-68	mol:protein length:398 Methionine gamma-lyase
rpsblast_cdd	gnl\|CDD\|162303	51	427	377	Gaps:5	100.00	378	66.67	11.38	0.0	TIGR01329 cysta_beta_ly_E cystathionine beta-lyase eukaryotic. This model represents cystathionine beta-lyase (alternate name: beta-cystathionase) one of several pyridoxal-dependent enzymes of cysteine methionine and homocysteine metabolism. This enzyme is involved in the biosynthesis of Met from Cys.
	gnl\|CDD\|144590	52	422	371	Gaps:13	99.74	381	45.26	15.00	1e-138	pfam01053 Cys_Met_Meta_PP Cys/Met metabolism PLP-dependent enzyme. This family includes enzymes involved in cysteine and methionine metabolism. The following are members: Cystathionine gamma-lyase Cystathionine gamma-synthase Cystathionine beta-lyase Methionine gamma-lyase OAH/OAS sulfhydrylase O-succinylhomoserine sulfhydrylase All of these members participate is slightly different reactions. All these enzymes use PLP (pyridoxal-5'-phosphate) as a cofactor.
	gnl\|CDD\|166150	47	422	376	Gaps:5	81.43	463	51.99	18.30	1e-114	PLN02509 PLN02509 cystathionine beta-lyase.
	gnl\|CDD\|99738	65	422	358	Gaps:14	99.73	369	44.57	15.76	1e-113	cd00614 CGS_like CGS_like: Cystathionine gamma-synthase is a PLP dependent enzyme and catalyzes the committed step of methionine biosynthesis. This pathway is unique to microorganisms and plants rendering the enzyme an attractive target for the development of antimicrobials and herbicides. This subgroup also includes cystathionine gamma-lyases (CGL) O-acetylhomoserine sulfhydrylases and O-acetylhomoserine thiol lyases. CGL's are very similar to CGS's. Members of this group are widely distributed among all three forms of life..
	gnl\|CDD\|30971	47	425	379	Gaps:17	98.99	396	42.35	17.35	1e-107	COG0626 MetC Cystathionine beta-lyases/cystathionine gamma-synthases [Amino acid transport and metabolism].
	gnl\|CDD\|169054	65	422	358	Gaps:4	94.96	377	45.25	18.99	1e-100	PRK07671 PRK07671 cystathionine beta-lyase Provisional.
	gnl\|CDD\|169112	59	425	367	Gaps:10	94.34	389	43.87	15.26	9e-95	PRK07811 PRK07811 cystathionine gamma-synthase Provisional.
	gnl\|CDD\|169205	47	422	376	Gaps:6	96.92	390	39.95	19.05	4e-90	PRK08064 PRK08064 cystathionine beta-lyase Provisional.
	gnl\|CDD\|168440	65	422	358	Gaps:4	94.46	379	41.90	18.72	2e-87	PRK06176 PRK06176 cystathionine gamma-synthase/cystathionine beta-lyase Validated.
	gnl\|CDD\|168478	44	422	379	Gaps:15	98.50	400	39.59	18.27	5e-85	PRK06234 PRK06234 methionine gamma-lyase Provisional.
rpsblast_kog	gnl\|CDD\|35276	39	425	387	Gaps:17	97.31	409	38.44	14.57	1e-102	KOG0053 KOG0053 KOG0053 Cystathionine beta-lyases/cystathionine gamma-synthases [Amino acid transport and metabolism].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	YCOA_554568	348875	349523	649	99.69	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCOA_551873	348875	349514	640	99.69	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCKA_560138	349623	349792	170	99.41	100.00	n/a	Botrytis cinerea HortResearch libraries
	PD0ABA15YH18FM1	349246	349908	663	100.00	100.00	n/a	BT4 young mycelium library
	PD0AGA9YN10CM1	348808	349439	632	100.00	100.00	n/a	BT4 mycelium - different stresses library
	B1BC_045_009_H08	348648	349330	689	97.53	100.00	n/a	BC ATCC 58025 (G3)
	PD0ACA23YN12FM1	348571	349362	792	100.00	100.00	n/a	BT4 mycelium - pH stress library
	YCKA_578999	349623	350360	687	99.71	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCKA_578526	349623	350300	629	99.84	100.00	n/a	Botrytis cinerea HortResearch libraries
	B3BC_116_233_D02	349633	350308	627	99.68	100.00	n/a	B0510 Germinated spores on hydrophobic surfaces library
	PD0ADA10YC22CM1	349602	350288	638	99.84	100.00	n/a	B0510 mycelium - carbon sources library
	PD0ADA4YJ05CM1	349819	350306	439	99.77	100.00	n/a	B0510 mycelium - carbon sources library
sim4_Bot_allest4_clctg	CL_bt4_SuperContig_330_1_bt4ctg_1632_006.Contig1_v5	348571	350308	1689	99.94	100.00	n/a	all_lib_clone_nr:7
sim4_Ss_allest	G787P59RH2.T0	348669	349366	823	86.84	84.93	n/a	Ss ESTs grown as developing apothecia
	G787P536RC2.T0	348669	349357	839	87.05	81.88	n/a	Ss ESTs grown as developing apothecia
	G787P59FH2.T0	349419	350246	875	89.19	88.80	n/a	Ss ESTs grown as developing apothecia
blastx_uniprot_sprot	Q9FPQ6	348830	348862	555	62.07	57.48	n/a	Vegetative cell wall protein gp1 precursor - Chlamydomonas reinhardtii
	P53780	348881	350005	464	51.33	81.03	n/a	Cystathionine beta-lyase chloroplast precursor - Arabidopsis thaliana (Mouse-ear cress)
	P53101	348884	349999	465	50.38	85.81	n/a	Cystathionine beta-lyase - Saccharomyces cerevisiae (Baker's yeast)
	P56069	348926	349996	380	42.58	93.95	n/a	Cystathionine gamma-synthase - Helicobacter pylori (Campylobacter pylori)
	A2RM21	348926	349999	380	42.74	94.21	n/a	Cystathionine beta-lyase - Lactococcus lactis subsp. cremoris (strain MG1363)
	P0A4K2	348926	349999	380	42.74	94.21	n/a	Cystathionine beta-lyase - Lactococcus lactis subsp. lactis (Streptococcus lactis)
	P0C2T9	348881	349999	380	41.33	98.68	n/a	Cystathionine beta-lyase - Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
	Q9ZMW7	348926	349996	380	41.46	93.95	n/a	Cystathionine gamma-synthase - Helicobacter pylori J99 (Campylobacter pylori J99)
	Q58DW2	348854	349999	405	38.82	96.05	n/a	Cystathionine gamma-lyase - Bos taurus (Bovine)
	P55216	348941	349999	392	39.50	91.07	n/a	Putative cystathionine gamma-lyase - Caenorhabditis elegans
	Q19QT7	348887	349999	405	38.56	92.84	n/a	Cystathionine gamma-lyase - Sus scrofa (Pig)
blastx_uniprot_trembl	Q9UV02	348734	350159	459	100.00	100.00	n/a	Cystathionine beta-lyase - Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea)
	Q96VU8	348734	350156	457	82.42	99.56	n/a	Cystathionine beta-lyase - Neurospora crassa
	Q2H105	348779	350099	444	85.41	95.72	n/a	Putative uncharacterized protein - Chaetomium globosum (Soil fungus)
	A4RHJ5	348797	350129	454	84.65	94.71	n/a	Putative uncharacterized protein - Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea)
	A1D9N1	348740	350093	443	78.98	97.74	n/a	Cystathionine beta-lyase MetG - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	A4DA77	348740	350093	443	78.98	97.74	n/a	Cystathionine beta-lyase MetG - Aspergillus fumigatus (Sartorya fumigata)
	Q2U0S6	348740	350093	441	77.70	98.64	n/a	Cystathionine beta-lyases/cystathionine gamma-synthases - Aspergillus oryzae
	Q9UV02	348734	350159	459	100.00	100.00	n/a	Cystathionine beta-lyase - Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea)
	Q96VU8	348734	350156	457	82.42	99.56	n/a	Cystathionine beta-lyase - Neurospora crassa
	Q1DLY1	348728	350117	458	77.85	97.60	n/a	Putative uncharacterized protein - Coccidioides immitis
blastx_FNG30_prot	SS1G_09924.1	348734	350159	459	96.73	100.00	n/a	sclerotinia_sclerotiorum hypothetical protein similar to cystathionine beta-lyase translation 460 aa
	jgi:Triree:18248	348812	350099	415	86.51	100.00	n/a	Trichoderma reesei
	NCU07987.2	348734	350156	457	82.42	99.56	n/a	Neurospora crassa hypothetical protein AF401237 cystathionine beta-lyase [Neurospora crassa] translation 458 aa
	CHG04541.1	348779	350099	444	85.41	95.72	n/a	Chaetomium globosum hypothetical protein similar to cystathionine beta-lyase translation 445 aa
	FG01932.1	348821	350129	436	83.41	96.79	n/a	fusarium_graminearum hypothetical protein similar to AF401237_1 cystathionine beta-lyase 437 aa
	MGG_07074.5	348797	350129	454	84.65	94.71	n/a	magnaporthe_grisea hypothetical protein similar to cystathionine beta-lyase translation 455 aa
	AO090011000323	348740	350093	441	77.70	98.64	n/a	Aspergillus oryzae cystathionine beta-lyases/cystathionine gamma-synthases
	AN7051	348779	350002	458	79.41	89.08	n/a	Aspergillus nidulans AN7051.3 hypothetical protein similar to cystathionine beta-lyase translation 459 aa
	gi:70982107:ref:XP_746582.1:	348740	349999	750	78.71	55.73	n/a	Aspergillus fumigatus Af293 hypothetical protein Afu4g03950
	SS1G_09924.1	348734	350159	459	96.73	100.00	n/a	sclerotinia_sclerotiorum hypothetical protein similar to cystathionine beta-lyase translation 460 aa
	NCU07987.2	348734	350156	457	82.42	99.56	n/a	Neurospora crassa hypothetical protein AF401237 cystathionine beta-lyase [Neurospora crassa] translation 458 aa
	CIMG_08682.2	348728	350117	458	77.85	97.60	n/a	Coccidioides immitis hypothetical protein similar to cystathionine beta-lyase translation 459 aa
sim4_B0510_genes	BC1G_02862.1	348734	350162	1380	99.71	100.00	n/a	n/a
sim4_SS_genes	SS1G_09924.1	348734	350142	1380	89.08	93.55	n/a	n/a
sim4_SS_genes	SS1G_09924.1	348734	350142	1380	89.08	93.55	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.9	344572	350311	532198	98.92	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.14	348733	350299	1192843	87.49	n/a	n/a	n/a
blat_oligo	botrytisP00126427	348741	348800	60	n/a	n/a	n/a	array_gene:BofuT4_P117340_1 . max_over_threshold:30.41 . min_over_threshold:4.2 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00126428	348868	348927	60	n/a	n/a	n/a	array_gene:BofuT4_P117340_1 . max_over_threshold:34.26 . min_over_threshold:2.67 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00126429	348877	348936	60	n/a	n/a	n/a	array_gene:BofuT4_P117340_1 . max_over_threshold:32.62 . min_over_threshold:2.93 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00126430	348985	349044	60	n/a	n/a	n/a	array_gene:BofuT4_P117340_1 . max_over_threshold:50.51 . min_over_threshold:4.53 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00126431	349154	349213	60	n/a	n/a	n/a	array_gene:BofuT4_P117340_1 . max_over_threshold:69.37 . min_over_threshold:8.02 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00126432	349331	349390	60	n/a	n/a	n/a	array_gene:BofuT4_P117340_1 . max_over_threshold:58.36 . min_over_threshold:7.87 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00126433	349403	349462	60	n/a	n/a	n/a	array_gene:BofuT4_P117340_1 . max_over_threshold:85.83 . min_over_threshold:15.9 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00126435	349683	349742	60	n/a	n/a	n/a	array_gene:BofuT4_P117340_1 . max_over_threshold:70.54 . min_over_threshold:7.9 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00126436	349913	349972	60	n/a	n/a	n/a	array_gene:BofuT4_P117340_1 . max_over_threshold:71.26 . min_over_threshold:6.31 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog