GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P101380.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_15506	11	1120	1110	n/a	100.00	1110	94.68	0.00	0.0	hypothetical protein K03235 elongation factor EF-3
	ssl:SS1G_04907	11	1120	1110	n/a	100.00	1110	87.03	3.96	0.0	elongation factor 3 K03235 elongation factor EF-3
	mgr:MGG_02572	11	1120	1110	Gaps:25	99.73	1118	71.30	9.69	0.0	MG02572.4 hypothetical protein K03235 elongation factor EF-3
	fgr:FG08532.1	25	1119	1095	Gaps:10	98.37	1103	72.17	9.77	0.0	hypothetical protein K03235 elongation factor EF-3
	mgr:MGCH7_ch7g285	11	1120	1110	Gaps:56	99.72	1087	71.96	9.96	0.0	MGG_ch7g285 hypothetical protein
	ncr:NCU09903	11	1119	1109	Gaps:21	99.64	1110	68.54	11.57	0.0	similar to mRNA-nucleus export ATPase K03235 elongation factor EF-3
	act:ACLA_096940	11	1120	1110	Gaps:13	99.91	1108	68.29	11.56	0.0	mRNA-nucleus export ATPase (Elf1) putative K03235 elongation factor EF-3
	afm:AFUA_6G03580	1	1120	1120	Gaps:14	99.91	1115	68.49	11.67	0.0	mRNA-nucleus export ATPase (Elf1) (EC:3.6.3.-) K01552 [EC:3.6.3.-]
	afv:AFLA_049300	1	1120	1120	Gaps:24	100.00	1126	67.58	11.28	0.0	mRNA-nucleus export ATPase (Elf1) putative K03235 elongation factor EF-3
	nfi:NFIA_050090	12	1120	1109	Gaps:11	99.64	1110	68.90	11.03	0.0	mRNA-nucleus export ATPase (Elf1) putative K03235 elongation factor EF-3
blastp_uniprot_sprot	sp\|Q08972\|NEW1_YEAST	58	1119	1062	Gaps:66	85.45	1196	50.88	15.95	0.0	[NU+] prion formation protein 1 OS Saccharomyces cerevisiae GN NEW1 PE 1 SV 1
	sp\|O14134\|ELF1_SCHPO	26	1119	1094	Gaps:111	98.86	1057	46.89	16.27	0.0	mRNA export factor elf1 OS Schizosaccharomyces pombe GN elf1 PE 1 SV 1
	sp\|O94489\|EF3_SCHPO	119	1035	917	Gaps:63	85.20	1047	41.03	19.39	1e-178	Elongation factor 3 OS Schizosaccharomyces pombe GN tef3 PE 1 SV 1
	sp\|P53978\|EF3B_YEAST	118	1030	913	Gaps:63	84.87	1044	42.55	17.83	1e-176	Elongation factor 3B OS Saccharomyces cerevisiae GN HEF3 PE 1 SV 2
	sp\|Q75EV6\|EF3_ASHGO	119	1035	917	Gaps:97	85.25	1044	43.48	16.63	1e-176	Elongation factor 3 OS Ashbya gossypii GN TEF3 PE 3 SV 1
	sp\|P25997\|EF3_CANAL	129	1030	902	Gaps:70	83.51	1049	42.92	17.24	1e-175	Elongation factor 3 OS Candida albicans GN TEF3 PE 1 SV 1
	sp\|O93796\|EF3_CANGA	119	1035	917	Gaps:65	85.17	1045	42.58	16.97	1e-175	Elongation factor 3 OS Candida glabrata GN TEF3 PE 3 SV 2
	sp\|P16521\|EF3A_YEAST	119	1035	917	Gaps:65	85.25	1044	41.46	17.98	1e-173	Elongation factor 3A OS Saccharomyces cerevisiae GN YEF3 PE 1 SV 3
	sp\|P29551\|EF3_PNECA	129	1030	902	Gaps:67	83.97	1042	40.11	19.31	1e-171	Elongation factor 3 OS Pneumocystis carinii GN TEF3 PE 3 SV 1
	sp\|Q8T6B7\|ABCF2_DICDI	513	1030	518	Gaps:94	80.44	593	33.75	17.19	1e-33	ABC transporter F family member 2 OS Dictyostelium discoideum GN abcF2 PE 3 SV 1
blastp_pdb	2ix3_B	119	1035	917	Gaps:65	90.26	986	41.46	17.98	1e-174	mol:protein length:986 ELONGATION FACTOR 3
	2ix3_A	119	1035	917	Gaps:65	90.26	986	41.46	17.98	1e-174	mol:protein length:986 ELONGATION FACTOR 3
	2iwh_B	119	1035	917	Gaps:65	90.26	986	41.46	17.98	1e-174	mol:protein length:986 ELONGATION FACTOR 3A
	2iwh_A	119	1035	917	Gaps:65	90.26	986	41.46	17.98	1e-174	mol:protein length:986 ELONGATION FACTOR 3A
	2iw3_B	119	1035	917	Gaps:65	90.26	986	41.46	17.98	1e-174	mol:protein length:986 ELONGATION FACTOR 3A
	2iw3_A	119	1035	917	Gaps:65	90.26	986	41.46	17.98	1e-174	mol:protein length:986 ELONGATION FACTOR 3A
	2ix8_A	119	1030	912	Gaps:65	90.68	976	41.58	17.97	1e-173	mol:protein length:976 ELONGATION FACTOR 3A
rpsblast_cdd	gnl\|CDD\|30834	488	1032	545	Gaps:102	97.55	530	26.89	17.02	3e-80	COG0488 Uup ATPase components of ABC transporters with duplicated ATPase domains [General function prediction only].
	gnl\|CDD\|72980	488	1021	534	Gaps:51	100.00	144	61.11	18.06	3e-35	cd03221 ABCF_EF-3 ABCF_EF-3 Elongation factor 3 (EF-3) is a cytosolic protein required by fungal ribosomes for in vitro protein synthesis and for in vivo growth. EF-3 stimulates the binding of the EF-1: GTP: aa-tRNA ternary complex to the ribosomal A site by facilitated release of the deacylated tRNA from the E site. The reaction requires ATP hydrolysis. EF-3 contains two ATP nucleotide binding sequence (NBS) motifs. NBSI is sufficient for the intrinsic ATPase activity. NBSII is essential for the ribosome-stimulated functions..
	gnl\|CDD\|170588	488	1026	539	Gaps:124	80.09	638	35.81	17.61	7e-33	PRK10636 PRK10636 putative ABC transporter ATP-binding protein Provisional.
	gnl\|CDD\|170982	487	1011	525	Gaps:83	74.65	635	38.40	17.72	3e-28	PRK11147 PRK11147 ABC transporter ATPase component Reviewed.
	gnl\|CDD\|31326	488	1033	546	Gaps:36	97.95	293	28.57	20.21	9e-25	COG1131 CcmA ABC-type multidrug transport system ATPase component [Defense mechanisms].
	gnl\|CDD\|72971	491	1025	535	Gaps:44	100.00	157	48.41	17.20	2e-23	cd00267 ABC_ATPase ABC (ATP-binding cassette) transporter nucleotide-binding domain ABC transporters are a large family of proteins involved in the transport of a wide variety of different compounds like sugars ions peptides and more complex organic molecules. The nucleotide-binding domain shows the highest similarity between all members of the family. ABC transporters are a subset of nucleotide hydrolases that contain a signature motif Q-loop and H-loop/switch region in addition to the Walker A motif/P-loop and Walker B motif commonly found in a number of ATP- and GTP-binding and hydrolyzing proteins..
	gnl\|CDD\|72984	487	1025	539	Gaps:47	100.00	211	44.08	17.06	1e-22	cd03225 ABC_cobalt_CbiO_domain1 Domain I of the ABC component of a cobalt transport family found in bacteria archaea and eukaryota. The transition metal cobalt is an essential component of many enzymes and must be transported into cells in appropriate amounts when needed. This ABC transport system of the CbiMNQO family is involved in cobalt transport in association with the cobalamin (vitamin B12) biosynthetic pathways. Most of cobalt (Cbi) transport systems possess a separate CbiN component the cobalt-binding periplasmic protein and they are encoded by the conserved gene cluster cbiMNQO. Both the CbiM and CbiQ proteins are integral cytoplasmic membrane proteins and the CbiO protein has the linker peptide and the Walker A and B motifs commonly found in the ATPase components of the ABC-type transport systems..
	gnl\|CDD\|72989	492	1026	535	Gaps:28	97.69	173	39.05	18.34	4e-22	cd03230 ABC_DR_subfamily_A This family of ATP-binding proteins belongs to a multisubunit transporter involved in drug resistance (BcrA and DrrA) nodulation lipid transport and lantibiotic immunity. In bacteria and archaea these transporters usually include an ATP-binding protein and one or two integral membrane proteins. Eukaryote systems of the ABCA subfamily display ABC domains that are quite similar to this family. The ATP-binding domain shows the highest similarity between all members of the ABC transporter family. ABC transporters are a subset of nucleotide hydrolases that contain a signature motif Q-loop and H-loop/switch region in addition to the Walker A motif/P-loop and Walker B motif commonly found in a number of ATP- and GTP-binding and hydrolyzing proteins..
	gnl\|CDD\|72994	491	1017	527	Gaps:38	95.77	213	39.71	22.06	5e-22	cd03235 ABC_Metallic_Cations ABC component of the metal-type transporters. This family includes transporters involved in the uptake of various metallic cations such as iron manganese and zinc. The ATPases of this group of transporters are very similar to members of iron-siderophore uptake family suggesting that they share a common ancestor. The best characterized metal-type ABC transporters are the YfeABCD system of Y. pestis the SitABCD system of Salmonella enterica serovar Typhimurium and the SitABCD transporter of Shigella flexneri. Moreover other uncharacterized homologs of these metal-type transporters are mainly found in pathogens like Haemophilus or enteroinvasive E. coli isolates..
	gnl\|CDD\|73027	497	673	177	Gaps:28	89.90	208	31.02	15.51	7e-22	cd03268 ABC_BcrA_bacitracin_resist The BcrA subfamily represents ABC transporters involved in peptide antibiotic resistance. Bacitracin is a dodecapeptide antibiotic produced by B. licheniformis and B. subtilis. The synthesis of bacitracin is non-ribosomally catalyzed by a multienzyme complex BcrABC. Bacitracin has potent antibiotic activity against gram-positive bacteria. The inhibition of peptidoglycan biosynthesis is the best characterized bacterial effect of bacitracin. The bacitracin resistance of B. licheniformis is mediated by the ABC transporter Bcr which is composed of two identical BcrA ATP-binding subunits and one each of the integral membrane proteins BcrB and BcrC. B. subtilis cells carrying bcr genes on high-copy number plasmids develop collateral detergent sensitivity a similar phenomenon in human cells with overexpressed multi-drug resistance P-glycoprotein..
rpsblast_kog	gnl\|CDD\|35285	410	1027	618	Gaps:90	96.74	582	46.71	15.99	1e-158	KOG0062 KOG0062 KOG0062 ATPase component of ABC transporters with duplicated ATPase domains/Translation elongation factor EF-3b [Amino acid transport and metabolism Translation ribosomal structure and biogenesis].
	gnl\|CDD\|36456	6	490	485	Gaps:46	80.67	569	27.89	19.39	3e-46	KOG1242 KOG1242 KOG1242 Protein containing adaptin N-terminal region [Translation ribosomal structure and biogenesis].
	gnl\|CDD\|36145	446	1027	582	Gaps:117	86.48	614	37.66	16.57	2e-44	KOG0927 KOG0927 KOG0927 Predicted transporter (ABC superfamily) [General function prediction only].
	gnl\|CDD\|35289	451	1030	580	Gaps:183	67.78	807	40.40	18.65	4e-44	KOG0066 KOG0066 KOG0066 eIF2-interacting protein ABC50 (ABC superfamily) [Translation ribosomal structure and biogenesis].
	gnl\|CDD\|35278	441	1006	566	Gaps:76	35.83	1228	29.32	19.32	6e-15	KOG0055 KOG0055 KOG0055 Multidrug/pheromone exporter ABC superfamily [Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|35280	461	1038	578	Gaps:53	44.67	591	29.55	17.80	5e-13	KOG0057 KOG0057 KOG0057 Mitochondrial Fe/S cluster exporter ABC superfamily [Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35284	502	649	148	Gaps:28	27.73	613	24.71	18.24	3e-09	KOG0061 KOG0061 KOG0061 Transporter ABC superfamily (Breast cancer resistance protein) [Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|37566	731	789	59	Gaps:1	19.93	291	32.76	24.14	9e-07	KOG2355 KOG2355 KOG2355 Predicted ABC-type transport ATPase component/CCR4 associated factor [General function prediction only Transcription].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	YCMA_553181	302753	303446	693	99.86	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCOA_570613	301174	301789	616	99.84	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCKA_555001	302705	302834	128	99.22	100.00	n/a	Botrytis cinerea HortResearch libraries
	B5BC_116_285_G11	301012	301727	716	98.88	100.00	n/a	B0510 In planta material
	B5BC_116_306_D01	301294	301729	436	99.54	100.00	n/a	B0510 In planta material
	B1BC_045_012_G09	300756	301450	698	98.71	100.00	n/a	BC ATCC 58025 (G3)
	B1BC_045_012_G08	300805	301450	646	99.38	100.00	n/a	BC ATCC 58025 (G3)
	PD0ABA38YB05FM1	300969	301546	578	99.83	100.00	n/a	BT4 young mycelium library
	PD0ACA14YE19FM1	302060	302756	697	99.86	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA10YK12FM1	300899	301557	659	99.85	100.00	n/a	BT4 mycelium - pH stress library
	PD0AHA13YG06CM1	300864	301547	684	100.00	100.00	n/a	BT4 appressoria on teflon library
	PD0AHA19YK16CM1	300788	301476	689	99.71	100.00	n/a	BT4 appressoria on teflon library
	YCKA_563986	300237	300922	632	99.84	100.00	n/a	Botrytis cinerea HortResearch libraries
	B3BC_116_219_C03	300231	300760	475	99.37	100.00	n/a	B0510 Germinated spores on hydrophobic surfaces library
	B5BC_116_298_G02	300227	300662	436	99.54	100.00	n/a	B0510 In planta material
	PD0ADA7YI01CM1	300585	301305	667	100.00	100.00	n/a	B0510 mycelium - carbon sources library
	PD0ACA7YJ11FM1	300231	300892	608	99.84	100.00	n/a	BT4 mycelium - pH stress library
	PD0AGA11YM19CM1	300236	300865	576	99.83	100.00	n/a	BT4 mycelium - different stresses library
	YCGA_532753	303722	304310	589	99.66	100.00	n/a	Botrytis cinerea HortResearch libraries
sim4_Bot_allest4_clctg	PD0ACA14YE19FM1	302060	302756	697	99.86	100.00	n/a	BT4 mycelium - pH stress library
sim4_Bot_allest4_clctg	CL_bt4_SuperContig_173_1_bt4ctg_1379_001.Contig1_v5	300227	301729	1449	99.93	100.00	n/a	all_lib_clone_nr:13
sim4_Ss_allest	G786P548RC5.T0	300790	301343	795	89.89	69.69	n/a	Ss ESTs grown as mycelia at neutral pH
sim4_Ss_allest	G786P548FC5.T0	300394	301169	870	88.52	59.08	n/a	Ss ESTs grown as mycelia at neutral pH
blastx_uniprot_sprot	O14134	300717	303953	1057	50.58	97.26	n/a	mRNA export factor elf1 - Schizosaccharomyces pombe (Fission yeast)
	Q08972	300618	303857	1196	54.01	85.45	n/a	[NU+] prion formation protein 1 - Saccharomyces cerevisiae (Baker's yeast)
	O93796	300747	303674	1045	43.35	90.72	n/a	Elongation factor 3 - Candida glabrata (Yeast) (Torulopsis glabrata)
	Q75EV6	300747	303674	1044	43.25	90.80	n/a	Elongation factor 3 - Ashbya gossypii (Yeast) (Eremothecium gossypii)
	P25997	300747	303644	1049	43.30	89.61	n/a	Elongation factor 3 - Candida albicans (Yeast)
	P53978	300756	303677	1044	42.95	90.33	n/a	Elongation factor 3B - Saccharomyces cerevisiae (Baker's yeast)
	P16521	300747	303674	1044	42.30	90.80	n/a	Elongation factor 3A - Saccharomyces cerevisiae (Baker's yeast)
	O94489	300750	303674	1047	41.62	90.64	n/a	Elongation factor 3 - Schizosaccharomyces pombe (Fission yeast)
	P29551	300939	303644	1042	41.71	83.97	n/a	Elongation factor 3 - Pneumocystis carinii
	Q6MG08	301836	302678	839	36.76	32.42	n/a	ATP-binding cassette sub-family F member 1 - Rattus norvegicus (Rat)
blastx_uniprot_trembl	A4RCI9	300615	303998	1118	75.78	99.73	n/a	Putative uncharacterized protein - Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea)
	Q2H3M2	300618	303992	1113	75.50	99.73	n/a	Putative uncharacterized protein - Chaetomium globosum (Soil fungus)
	Q2KGQ0	300765	303998	1087	74.52	99.63	n/a	Putative uncharacterized protein - Magnaporthe grisea 70-15
	A1DLJ6	300615	303995	1110	73.33	99.64	n/a	MRNA-nucleus export ATPase - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q4WD57	300615	304028	1115	72.89	99.91	n/a	MRNA-nucleus export ATPase - Aspergillus fumigatus (Sartorya fumigata)
	Q0CGS2	300615	303998	1108	72.63	99.91	n/a	Putative uncharacterized protein - Aspergillus terreus (strain NIH 2624)
	Q7S0E4	300618	303998	1110	72.60	99.64	n/a	Putative uncharacterized protein - Neurospora crassa
	A2R4X2	300618	304028	1117	72.31	99.91	n/a	Function: the translational elongation factor 3 - Aspergillus niger
blastx_FNG30_prot	SS1G_04907.1	300615	303998	1110	92.16	100.00	n/a	sclerotinia_sclerotiorum elongation factor 3 translation 1111 aa
	MGG_02572.5	300615	303998	1118	75.78	99.73	n/a	magnaporthe_grisea hypothetical protein translation 1119 aa
	CHG06743.1	300618	303992	1113	75.50	99.73	n/a	Chaetomium globosum hypothetical protein translation 1114 aa
	FG08532.1	300618	303959	1103	76.15	98.46	n/a	fusarium_graminearum hypothetical protein 1104 aa
	jgi:Triree:45426	300618	304028	1105	75.00	99.91	n/a	Trichoderma reesei
	gi:70984412:ref:XP_747719.1:	300615	304028	1115	72.89	99.91	n/a	Aspergillus fumigatus Af293 mRNA-nucleus export ATPase
	NCU09903.2	300618	303998	1110	72.60	99.64	n/a	Neurospora crassa hypothetical protein translation 1111 aa
	AO090009000567	300615	303998	1114	72.26	100.00	n/a	Aspergillus oryzae ATPase component of ABC transporters with duplicated ATPase domains/Translation elongation factor EF-3b
	CIMG_10089.2	300618	304007	1109	71.67	99.64	n/a	Coccidioides immitis hypothetical protein translation 1110 aa
	AN6651	300615	304028	1118	71.35	99.91	n/a	Aspergillus nidulans AN6651.3 conserved hypothetical protein translation 1119 aa
sim4_B0510_genes	BC1G_15506.1	300612	303998	3333	99.94	100.00	n/a	n/a
	BC1G_15506.1	300612	303998	3333	99.94	100.00	n/a	n/a
	BC1G_05784.1	300764	307690	591	92.86	18.95	n/a	n/a
	BC1G_13888.1	300768	307684	546	83.81	19.23	n/a	n/a
sim4_SS_genes	SS1G_04907.1	300757	303998	3333	88.62	94.69	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.190	300209	300767	54273	99.82	n/a	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.190	300795	305554	54273	99.68	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.6	300796	302770	2040374	89.01	n/a	n/a	n/a
	SS_supercontig_1.6	302893	304031	2040374	87.62	n/a	n/a	n/a
	SS_supercontig_1.6	302893	304031	2040374	87.62	n/a	n/a	n/a
blat_oligo	botrytisP00113989	302063	302122	60	n/a	n/a	n/a	array_gene:BofuT4_P101380_1 . max_over_threshold:123.53 . min_over_threshold:9 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00113990	301952	302011	60	n/a	n/a	n/a	array_gene:BofuT4_P101380_1 . max_over_threshold:83.06 . min_over_threshold:5.02 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00113991	301789	301848	60	n/a	n/a	n/a	array_gene:BofuT4_P101380_1 . max_over_threshold:73.54 . min_over_threshold:3.26 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00113992	301624	301683	60	n/a	n/a	n/a	array_gene:BofuT4_P101380_1 . max_over_threshold:50.86 . min_over_threshold:2.13 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00113993	301516	301575	60	n/a	n/a	n/a	array_gene:BofuT4_P101380_1 . max_over_threshold:60.61 . min_over_threshold:2.29 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00113994	301253	301312	60	n/a	n/a	n/a	array_gene:BofuT4_P101380_1 . max_over_threshold:65.85 . min_over_threshold:2.7 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00113995	301028	301087	60	n/a	n/a	n/a	array_gene:BofuT4_P101380_1 . max_over_threshold:79.71 . min_over_threshold:3.44 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00113996	300911	300970	60	n/a	n/a	n/a	array_gene:BofuT4_P101380_1 . max_over_threshold:120.93 . min_over_threshold:6.28 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:1
	botrytisP00113998	300620	300733	60	n/a	n/a	n/a	array_gene:BofuT4_P101380_1 . max_over_threshold:89.22 . min_over_threshold:4.65 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog