GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P059190.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	ssl:SS1G_10281	1	298	298	n/a	100.00	298	90.60	2.35	1e-160	hypothetical protein
	bfu:BC1G_08982	1	282	282	n/a	11.90	2369	93.26	0.35	1e-154	hypothetical protein
	afm:AFUA_6G13230	1	291	291	n/a	99.66	292	72.16	11.68	1e-127	nitrilase family protein (Nit3)
	act:ACLA_086490	1	291	291	n/a	99.66	292	71.48	11.34	1e-125	nitrilase family protein (Nit3) putative
	nfi:NFIA_059160	1	291	291	n/a	99.66	292	71.48	11.34	1e-125	nitrilase family protein (Nit3) putative
	afv:AFLA_033700	1	291	291	n/a	99.66	292	70.79	12.03	1e-125	nitrilase family protein (Nit3) putative
	aor:AO090003000448	1	291	291	n/a	99.66	292	70.79	12.03	1e-125	carbon-nitrogen hydrolase
	ncr:NCU06726	4	290	287	Gaps:5	95.42	306	72.60	9.25	1e-124	similar to nit protein 2
	cim:CIMG_01655	1	298	298	Gaps:1	71.70	417	68.56	12.04	1e-122	hypothetical protein
	ang:An08g10150	14	293	280	n/a	95.56	293	72.86	9.29	1e-121	hypothetical protein
blastp_uniprot_sprot	sp\|Q10166\|YAUB_SCHPO	7	288	282	Gaps:13	84.78	322	58.24	14.65	2e-90	UPF0012 hydrolase C26A3.11 OS Schizosaccharomyces pombe GN SPAC26A3.11 PE 2 SV 1
	sp\|P49954\|NIT3_YEAST	2	289	288	Gaps:8	98.28	291	51.75	15.03	3e-80	Probable hydrolase NIT3 OS Saccharomyces cerevisiae GN NIT3 PE 1 SV 1
	sp\|Q497B0\|NIT2_RAT	10	285	276	Gaps:11	96.01	276	54.34	12.08	8e-76	Omega-amidase NIT2 OS Rattus norvegicus GN Nit2 PE 1 SV 1
	sp\|Q9JHW2\|NIT2_MOUSE	10	285	276	Gaps:11	96.01	276	54.72	11.70	2e-75	Omega-amidase NIT2 OS Mus musculus GN Nit2 PE 1 SV 1
	sp\|Q2T9R6\|NIT2_BOVIN	10	286	277	Gaps:11	96.38	276	53.01	14.66	6e-75	Omega-amidase NIT2 OS Bos taurus GN NIT2 PE 2 SV 1
	sp\|Q4VBV9\|NIT2_DANRE	10	285	276	Gaps:11	95.67	277	53.21	13.21	2e-74	Omega-amidase NIT2 OS Danio rerio GN nit2 PE 2 SV 1
	sp\|Q28IE5\|NIT2_XENTR	10	285	276	Gaps:11	96.01	276	52.08	14.72	3e-74	Omega-amidase NIT2 OS Xenopus tropicalis GN nit2 PE 2 SV 1
	sp\|Q6INI7\|NIT2B_XENLA	10	285	276	Gaps:11	96.01	276	52.83	13.21	1e-73	Omega-amidase NIT2-B OS Xenopus laevis GN nit2b PE 2 SV 1
	sp\|Q9NQR4\|NIT2_HUMAN	10	285	276	Gaps:11	96.01	276	52.45	15.09	2e-73	Omega-amidase NIT2 OS Homo sapiens GN NIT2 PE 1 SV 1
	sp\|Q5R4L6\|NIT2_PONAB	10	285	276	Gaps:12	96.00	275	52.27	14.39	3e-72	Omega-amidase NIT2 OS Pongo abelii GN NIT2 PE 3 SV 1
blastp_pdb	1f89_B	2	289	288	Gaps:8	98.28	291	51.75	15.03	6e-81	mol:protein length:291 32.5 KDA PROTEIN YLR351C
	1f89_A	2	289	288	Gaps:8	98.28	291	51.75	15.03	6e-81	mol:protein length:291 32.5 KDA PROTEIN YLR351C
	2w1v_B	10	285	276	Gaps:11	96.01	276	54.72	11.70	5e-76	mol:protein length:276 NITRILASE HOMOLOG 2
	2w1v_A	10	285	276	Gaps:11	96.01	276	54.72	11.70	5e-76	mol:protein length:276 NITRILASE HOMOLOG 2
	1ems_B	11	285	275	Gaps:10	61.14	440	30.48	17.47	3e-31	mol:protein length:440 NIT-FRAGILE HISTIDINE TRIAD FUSION PROTEIN
	1ems_A	11	285	275	Gaps:10	61.14	440	30.48	17.47	3e-31	mol:protein length:440 NIT-FRAGILE HISTIDINE TRIAD FUSION PROTEIN
	1j31_D	9	243	235	Gaps:23	81.68	262	28.97	20.56	1e-13	mol:protein length:262 Hypothetical protein PH0642
	1j31_C	9	243	235	Gaps:23	81.68	262	28.97	20.56	1e-13	mol:protein length:262 Hypothetical protein PH0642
	1j31_B	9	243	235	Gaps:23	81.68	262	28.97	20.56	1e-13	mol:protein length:262 Hypothetical protein PH0642
	1j31_A	9	243	235	Gaps:23	81.68	262	28.97	20.56	1e-13	mol:protein length:262 Hypothetical protein PH0642
rpsblast_cdd	gnl\|CDD\|143596	10	281	272	Gaps:7	100.00	265	49.81	16.60	1e-115	cd07572 nit Nit1 Nit 2 and related proteins and the Nit1-like domain of NitFhit (class 10 nitrilases). This subgroup includes mammalian Nit1 and Nit2 the Nit1-like domain of the invertebrate NitFhit and various uncharacterized bacterial and archaeal Nit-like proteins. Nit1 and Nit2 are candidate tumor suppressor proteins. In NitFhit the Nit1-like domain is encoded as a fusion protein with the non-homologous tumor suppressor fragile histidine triad (Fhit). Mammalian Nit1 and Fhit may affect distinct signal pathways and both may participate in DNA damage-induced apoptosis. Nit1 is a negative regulator in T cells. Overexpression of Nit2 in HeLa cells leads to a suppression of cell growth through cell cycle arrest in G2. These Nit proteins and the Nit1-like domain of NitFhit belong to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) this subgroup corresponds to class 10.
	gnl\|CDD\|143607	10	281	272	Gaps:37	100.00	253	39.53	15.81	2e-58	cd07583 nitrilase_5 Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases). The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
	gnl\|CDD\|143587	11	281	271	Gaps:20	100.00	253	39.13	14.62	4e-57	cd07197 nitrilase Nitrilase superfamily including nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes. This superfamily (also known as the C-N hydrolase superfamily) contains hydrolases that break carbon-nitrogen bonds it includes nitrilases cyanide dihydratases aliphatic amidases N-terminal amidases beta-ureidopropionases biotinidases pantotheinase N-carbamyl-D-amino acid amidohydrolases the glutaminase domain of glutamine-dependent NAD+ synthetase apolipoprotein N-acyltransferases and N-carbamoylputrescine amidohydrolases among others. These enzymes depend on a Glu-Lys-Cys catalytic triad and work through a thiol acylenzyme intermediate. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer. These oligomers include dimers tetramers hexamers octamers tetradecamers octadecamers as well as variable length helical arrangements and homo-oligomeric spirals. These proteins have roles in vitamin and co-enzyme metabolism in detoxifying small molecules in the synthesis of signaling molecules and in the post-translational modification of proteins. They are used industrially as biocatalysts in the fine chemical and pharmaceutical industry in cyanide remediation and in the treatment of toxic effluent. This superfamily has been classified previously in the literature based on global and structure-based sequence analysis into thirteen different enzyme classes (referred to as 1-13). This hierarchy includes those thirteen classes and a few additional subfamilies. A putative distant relative the plasmid-borne TraB family has not been included in the hierarchy.
	gnl\|CDD\|30737	7	286	280	Gaps:18	97.81	274	33.58	20.52	1e-53	COG0388 COG0388 Predicted amidohydrolase [General function prediction only].
	gnl\|CDD\|143605	11	281	271	Gaps:30	100.00	255	36.47	19.22	5e-50	cd07581 nitrilase_3 Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases). The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
	gnl\|CDD\|166439	7	283	277	Gaps:11	95.77	284	33.46	20.22	4e-44	PLN02798 PLN02798 nitrilase.
	gnl\|CDD\|143597	9	290	282	Gaps:42	100.00	284	33.10	16.90	1e-42	cd07573 CPA N-carbamoylputrescine amidohydrolase (CPA) (class 11 nitrilases). CPA (EC 3.5.1.53 also known as N-carbamoylputrescine amidase and carbamoylputrescine hydrolase) converts N-carbamoylputrescine to putrescine a step in polyamine biosynthesis in plants and bacteria. This subgroup includes Arabidopsis thaliana CPA also known as nitrilase-like 1 (NLP1) and Pseudomonas aeruginosa AguB. This subgroup belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) this subgroup corresponds to class 11. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer P. aeruginosa AugB is a homohexamer Arabidopsis thaliana NLP1 is a homooctomer.
	gnl\|CDD\|143608	10	281	272	Gaps:19	99.61	258	35.41	15.56	3e-34	cd07584 nitrilase_6 Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases). The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
	gnl\|CDD\|144405	10	194	185	Gaps:17	100.00	172	37.79	12.21	4e-34	pfam00795 CN_hydrolase Carbon-nitrogen hydrolase. This family contains hydrolases that break carbon-nitrogen bonds. The family includes: Nitrilase EC:3.5.5.1 Aliphatic amidase EC:3.5.1.4 Biotidinase EC:3.5.1.12 Beta-ureidopropionase EC:3.5.1.6. Nitrilase-related proteins generally have a conserved E-K-C catalytic triad and are multimeric alpha-beta-beta-alpha sandwich proteins.
	gnl\|CDD\|143600	10	283	274	Gaps:26	100.00	254	31.89	15.35	9e-33	cd07576 R-amidase_like Pseudomonas sp. MCI3434 R-amidase and related proteins (putative class 13 nitrilases). Pseudomonas sp. MCI3434 R-amidase hydrolyzes (R S)-piperazine-2-tert-butylcarboxamide to form (R)-piperazine-2-carboxylic acid. It does so with strict R-stereoselectively. Its preferred substrates are carboxamide compounds which have the amino or imino group connected to their beta- or gamma-carbon. This subgroup belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) class 13 represents proteins that at the time were difficult to place in a distinct similarity group. It has been suggested that this subgroup represents a new class. Members of the nitrilase superfamily generally form homomeric complexes the basic building block of which is a homodimer. Native R-amidase however appears to be a monomer.
rpsblast_kog	gnl\|CDD\|36024	1	286	286	Gaps:16	97.99	298	39.04	16.44	4e-76	KOG0806 KOG0806 KOG0806 Carbon-nitrogen hydrolase [Amino acid transport and metabolism].
	gnl\|CDD\|36025	7	285	279	Gaps:20	93.22	295	33.45	18.91	1e-47	KOG0807 KOG0807 KOG0807 Carbon-nitrogen hydrolase [Amino acid transport and metabolism].
	gnl\|CDD\|36023	9	271	263	Gaps:61	83.09	337	26.79	16.43	8e-15	KOG0805 KOG0805 KOG0805 Carbon-nitrogen hydrolase [Amino acid transport and metabolism].
	gnl\|CDD\|36026	10	219	210	Gaps:14	52.71	387	25.00	17.16	8e-08	KOG0808 KOG0808 KOG0808 Carbon-nitrogen hydrolase [Amino acid transport and metabolism].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	AL114270	504003	504426	424	100.00	100.00	n/a	BT4 Mycelium - nitrate starvation library
	PD0ABA1YC08NM1r	503892	504687	796	100.00	100.00	n/a	BT4 young mycelium library
	YCMA_575738	504218	504953	662	99.40	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCIA_545129	504321	504944	550	99.09	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCKA_569206	504502	504928	353	99.72	100.00	n/a	Botrytis cinerea HortResearch libraries
	PD0ADA8YO14CM1	504184	504949	692	99.57	100.00	n/a	B0510 mycelium - carbon sources library
	PD0ABA36YD12FM1	504178	504967	716	100.00	100.00	n/a	BT4 young mycelium library
	PD0ABA1YC08FM1	504332	504969	564	99.82	100.00	n/a	BT4 young mycelium library
	PD0AGA7YP16CM1	504697	504887	117	100.00	100.00	n/a	BT4 mycelium - different stresses library
	PD0AHA6YH24CM1	504320	504972	579	99.83	100.00	n/a	BT4 appressoria on teflon library
sim4_Bot_allest4_clctg	CL_bt4_SuperContig_51_1_bt4ctg_0757_004.Contig1_v5	503892	504972	1007	100.00	100.00	n/a	all_lib_clone_nr:6
sim4_Ss_allest	G786P58RC4.T0	504083	504875	776	88.89	92.78	n/a	Ss ESTs grown as mycelia at neutral pH
blastx_uniprot_sprot	Q10166	503932	504735	322	61.39	80.43	n/a	UPF0012 hydrolase C26A3.11 - Schizosaccharomyces pombe (Fission yeast)
	P49954	503929	504866	291	56.49	97.94	n/a	Probable hydrolase NIT3 - Saccharomyces cerevisiae (Baker's yeast)
	Q32LH4	503941	504747	328	36.23	80.79	n/a	Nitrilase homolog 1 - Bos taurus (Bovine)
	Q86X76	503941	504747	327	35.85	81.04	n/a	Nitrilase homolog 1 - Homo sapiens (Human)
	Q7TQ94	503941	504747	292	35.47	90.75	n/a	Nitrilase homolog 1 - Rattus norvegicus (Rat)
	O76463	503941	504747	440	31.94	59.77	n/a	Nitrilase and fragile histidine triad fusion protein NitFhit [Includes: Bis(5'-adenosyl)-triphosphatase - Caenorhabditis elegans
	Q8VDK1	503941	504747	323	35.09	82.04	n/a	Nitrilase homolog 1 - Mus musculus (Mouse)
	P55175	503950	504747	272	31.15	95.59	n/a	UPF0012 hydrolase sll0601 - Synechocystis sp. (strain PCC 6803)
	P47016	503941	504744	307	29.45	95.11	n/a	Probable hydrolase NIT2 - Saccharomyces cerevisiae (Baker's yeast)
	O76464	503941	504738	460	31.91	55.87	n/a	Nitrilase and fragile histidine triad fusion protein NitFhit - Drosophila melanogaster (Fruit fly)
blastx_uniprot_trembl	A2QSC0	503917	504869	293	76.45	100.00	n/a	Catalytic activity: a Nitrile + H2O a Carboxylate + NH3. precursor - Aspergillus niger
	Q4WLK2	503923	504869	292	75.60	99.66	n/a	Nitrilase family protein - Aspergillus fumigatus (Sartorya fumigata)
	Q2ULD8	503923	504869	292	75.26	99.66	n/a	Carbon-nitrogen hydrolase - Aspergillus oryzae
	A1CUG3	503923	504869	292	74.91	99.66	n/a	Nitrilase family protein - Aspergillus clavatus
	Q5B1U4	503935	504869	422	77.00	68.01	n/a	Putative uncharacterized protein - Emericella nidulans (Aspergillus nidulans)
blastx_FNG30_prot	SS1G_10281.1	503902	504869	298	95.64	100.00	n/a	sclerotinia_sclerotiorum hypothetical protein translation 299 aa
	gi:70992703:ref:XP_751200.1:	503923	504869	292	75.60	99.66	n/a	Aspergillus fumigatus Af293 hypothetical protein Afu6g13230
	AO090003000448	503923	504869	292	75.26	99.66	n/a	Aspergillus oryzae carbon-nitrogen hydrolase
	AN10675	503929	504869	293	77.16	98.63	n/a	Aspergillus nidulans AN10675.3 conserved hypothetical protein translation 294 aa
	NCU06726.2	503926	504860	306	77.05	95.42	n/a	Neurospora crassa hypothetical protein translation 307 aa
	CIMG_01655.2	503902	504869	417	71.57	71.70	n/a	Coccidioides immitis conserved hypothetical protein translation 418 aa
	FG10250.1	503926	504860	301	74.13	95.02	n/a	fusarium_graminearum hypothetical protein 302 aa
	SNU11542.1	503926	504869	296	71.03	97.97	n/a	Stagonospora nodorum hypothetical protein translation
	jgi:Triree:39878	503905	504881	305	72.43	98.69	n/a	Trichoderma reesei
	MGG_06196.5	503926	504881	307	70.33	97.72	n/a	magnaporthe_grisea hypothetical protein translation 308 aa
sim4_B0510_genes	no results
sim4_SS_genes	SS1G_10281.1	503899	504869	897	88.18	100.00	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.52	497250	506854	250757	99.58	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.15	504808	504872	1185750	92.31	n/a	n/a	n/a
	SS_supercontig_1.15	503895	504779	1185750	87.68	n/a	n/a	n/a
	SS_supercontig_1.15	504808	504872	1185750	92.31	n/a	n/a	n/a
blat_oligo	botrytisP00081853	504722	504855	60	n/a	n/a	n/a	array_gene:BofuT4_P059190_1 . max_over_threshold:40.85 . min_over_threshold:4.93 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081854	504509	504568	60	n/a	n/a	n/a	array_gene:BofuT4_P059190_1 . max_over_threshold:67.5 . min_over_threshold:9.52 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081855	504289	504348	60	n/a	n/a	n/a	array_gene:BofuT4_P059190_1 . max_over_threshold:62.01 . min_over_threshold:9.57 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081857	504181	504240	60	n/a	n/a	n/a	array_gene:BofuT4_P059190_1 . max_over_threshold:79.79 . min_over_threshold:9.66 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081858	504153	504212	60	n/a	n/a	n/a	array_gene:BofuT4_P059190_1 . max_over_threshold:93.89 . min_over_threshold:18.3 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081859	504119	504178	60	n/a	n/a	n/a	array_gene:BofuT4_P059190_1 . max_over_threshold:98.76 . min_over_threshold:22.96 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081860	504107	504166	60	n/a	n/a	n/a	array_gene:BofuT4_P059190_1 . max_over_threshold:94.78 . min_over_threshold:14.7 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081861	504094	504153	60	n/a	n/a	n/a	array_gene:BofuT4_P059190_1 . max_over_threshold:70.9 . min_over_threshold:12.26 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081862	503936	503995	60	n/a	n/a	n/a	array_gene:BofuT4_P059190_1 . max_over_threshold:75.1 . min_over_threshold:9.74 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog