GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P058790.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_08482	1	697	697	n/a	99.86	698	99.71	0.29	0.0	hypothetical protein K00232 acyl-CoA oxidase [EC:1.3.3.6]
	ssl:SS1G_10238	1	687	687	n/a	93.47	735	93.74	4.08	0.0	hypothetical protein K00232 acyl-CoA oxidase [EC:1.3.3.6]
	ure:UREG_05801	1	694	694	Gaps:3	100.00	693	72.44	13.13	0.0	hypothetical protein K00232 acyl-CoA oxidase [EC:1.3.3.6]
	cim:CIMG_07097	1	694	694	Gaps:3	100.00	693	72.44	12.70	0.0	hypothetical protein K00232 acyl-CoA oxidase [EC:1.3.3.6]
	afv:AFLA_077250	3	688	686	Gaps:3	98.41	694	72.77	13.03	0.0	fatty-acyl coenzyme A oxidase (Pox1) putative K00232 acyl-CoA oxidase [EC:1.3.3.6]
	aor:AO090005000479	3	688	686	Gaps:3	98.41	694	72.77	13.03	0.0	acyl-CoA oxidase K00232 acyl-CoA oxidase [EC:1.3.3.6]
	fgr:FG02287.1	4	689	686	Gaps:1	98.42	696	71.82	12.41	0.0	hypothetical protein K00232 acyl-CoA oxidase [EC:1.3.3.6]
	nfi:NFIA_027340	3	688	686	Gaps:4	98.55	692	71.70	12.02	0.0	acyl-CoA oxidase K00232 acyl-CoA oxidase [EC:1.3.3.6]
	pcs:Pc20g01800	3	689	687	Gaps:2	98.56	695	71.24	12.26	0.0	Pc20g01800 K00232 acyl-CoA oxidase [EC:1.3.3.6]
	ani:AN6752.2	3	688	686	Gaps:2	98.28	696	70.47	13.30	0.0	hypothetical protein K00232 acyl-CoA oxidase [EC:1.3.3.6]
blastp_uniprot_sprot	sp\|O65202\|ACOX1_ARATH	16	676	661	Gaps:59	96.69	664	39.41	17.91	1e-118	Peroxisomal acyl-coenzyme A oxidase 1 OS Arabidopsis thaliana GN ACX1 PE 1 SV 1
	sp\|Q9ZQP2\|ACO12_ARATH	24	698	675	Gaps:63	98.49	664	38.69	18.65	1e-116	Putative peroxisomal acyl-coenzyme A oxidase 1.2 OS Arabidopsis thaliana GN ACX1.2 PE 2 SV 1
	sp\|P07872\|ACOX1_RAT	21	676	656	Gaps:56	97.13	661	38.94	20.25	1e-112	Peroxisomal acyl-coenzyme A oxidase 1 OS Rattus norvegicus GN Acox1 PE 1 SV 1
	sp\|Q5RC19\|ACOX1_PONAB	21	672	652	Gaps:48	96.67	660	39.03	19.91	1e-110	Peroxisomal acyl-coenzyme A oxidase 1 OS Pongo abelii GN ACOX1 PE 2 SV 2
	sp\|Q15067\|ACOX1_HUMAN	21	672	652	Gaps:48	96.67	660	39.03	19.91	1e-110	Peroxisomal acyl-coenzyme A oxidase 1 OS Homo sapiens GN ACOX1 PE 1 SV 3
	sp\|Q9Z1N0\|ACOX1_CAVPO	21	672	652	Gaps:58	96.52	661	38.87	20.85	1e-110	Peroxisomal acyl-coenzyme A oxidase 1 OS Cavia porcellus GN ACOX1 PE 2 SV 1
	sp\|Q9R0H0\|ACOX1_MOUSE	21	676	656	Gaps:52	97.13	661	38.32	20.40	1e-110	Peroxisomal acyl-coenzyme A oxidase 1 OS Mus musculus GN Acox1 PE 1 SV 4
	sp\|Q8HYL8\|ACOX1_PHACI	21	672	652	Gaps:54	96.52	661	39.34	17.55	1e-109	Peroxisomal acyl-coenzyme A oxidase 1 OS Phascolarctos cinereus GN ACOX1 PE 1 SV 1
	sp\|Q3SZP5\|ACOX1_BOVIN	21	672	652	Gaps:54	96.67	660	38.71	19.59	1e-109	Peroxisomal acyl-coenzyme A oxidase 1 OS Bos taurus GN ACOX1 PE 2 SV 1
	sp\|Q54GQ6\|ACOX1_DICDI	19	685	667	Gaps:76	97.00	700	33.58	20.91	1e-96	Peroxisomal acyl-coenzyme A oxidase 1 OS Dictyostelium discoideum GN acox1 PE 3 SV 1
blastp_pdb	1w07_B	16	676	661	Gaps:59	97.42	659	39.41	17.76	1e-118	mol:protein length:659 ACYL-COA OXIDASE
	1w07_A	16	676	661	Gaps:59	97.42	659	39.41	17.76	1e-118	mol:protein length:659 ACYL-COA OXIDASE
	2fon_C	16	672	657	Gaps:43	93.41	683	38.56	21.00	1e-114	mol:protein length:683 peroxisomal acyl-CoA oxidase 1A
	2fon_B	16	672	657	Gaps:43	93.41	683	38.56	21.00	1e-114	mol:protein length:683 peroxisomal acyl-CoA oxidase 1A
	2fon_A	16	672	657	Gaps:43	93.41	683	38.56	21.00	1e-114	mol:protein length:683 peroxisomal acyl-CoA oxidase 1A
	2ddh_A	21	676	656	Gaps:52	97.13	661	39.10	19.00	1e-113	mol:protein length:661 Acyl-CoA oxidase
	1is2_B	21	676	656	Gaps:52	97.13	661	39.10	19.00	1e-113	mol:protein length:661 acyl-CoA oxidase
	1is2_A	21	676	656	Gaps:52	97.13	661	39.10	19.00	1e-113	mol:protein length:661 acyl-CoA oxidase
rpsblast_cdd	gnl\|CDD\|173839	20	667	648	Gaps:87	99.84	610	37.60	19.54	1e-147	cd01150 AXO Peroxisomal acyl-CoA oxidase. Peroxisomal acyl-CoA oxidases (AXO) catalyze the first set in the peroxisomal fatty acid beta-oxidation the alpha beta dehydrogenation of the corresponding trans-enoyl-CoA by FAD which becomes reduced. In a second oxidative half-reaction the reduced FAD is reoxidized by molecular oxygen. AXO is generally a homodimer but it has been reported to form a different type of oligomer in yeast. There are several subtypes of AXO's based on substrate specificity. Palmitoyl-CoA oxidase acts on straight-chain fatty acids and prostanoids whereas the closely related Trihydroxycoprostanoly-CoA oxidase has the greatest activity for 2-methyl branched side chains of bile precursors. Pristanoyl-CoA oxidase acts on 2-methyl branched fatty acids. AXO has an additional domain C-terminal to the region with similarity to acyl-CoA dehydrogenases which is included in this alignment.
	gnl\|CDD\|166084	24	672	649	Gaps:61	94.88	664	39.84	17.46	1e-130	PLN02443 PLN02443 acyl-CoA oxidase.
	gnl\|CDD\|166277	113	643	531	Gaps:67	75.87	688	34.87	17.82	1e-66	PLN02636 PLN02636 acyl-CoA oxidase.
	gnl\|CDD\|165953	113	643	531	Gaps:59	74.30	681	33.60	17.59	8e-64	PLN02312 PLN02312 acyl-CoA oxidase.
	gnl\|CDD\|145092	507	679	173	Gaps:14	93.01	186	33.53	20.81	2e-29	pfam01756 ACOX Acyl-CoA oxidase. This is a family of Acyl-CoA oxidases EC:1.3.3.6. Acyl-coA oxidase converts acyl-CoA into trans-2- enoyl-CoA.
	gnl\|CDD\|173838	120	468	349	Gaps:66	86.54	327	25.80	15.90	6e-27	cd00567 ACAD Acyl-CoA dehydrogenase. Both mitochondrial acyl-CoA dehydrogenases (ACAD) and peroxisomal acyl-CoA oxidases (AXO) catalyze the alpha beta dehydrogenation of the corresponding trans-enoyl-CoA by FAD which becomes reduced. The reduced form of ACAD is reoxidized in the oxidative half-reaction by electron-transferring flavoprotein (ETF) from which the electrons are transferred to the mitochondrial respiratory chain coupled with ATP synthesis. In contrast AXO catalyzes a different oxidative half-reaction in which the reduced FAD is reoxidized by molecular oxygen. The ACAD family includes the eukaryotic beta-oxidation enzymes short (SCAD) medium (MCAD) long (LCAD) and very-long (VLCAD) chain acyl-CoA dehydrogenases. These enzymes all share high sequence similarity but differ in their substrate specificities. The ACAD family also includes amino acid catabolism enzymes such as Isovaleryl-CoA dehydrogenase (IVD) short/branched chain acyl-CoA dehydrogenases(SBCAD) Isobutyryl-CoA dehydrogenase (IBDH) glutaryl-CoA deydrogenase (GCD) and Crotonobetainyl-CoA dehydrogenase. The mitochondrial ACAD's are generally homotetramers except for VLCAD which is a homodimer. Related enzymes include the SOS adaptive reponse proten aidB Naphthocyclinone hydroxylase (NcnH) and and Dibenzothiophene (DBT) desulfurization enzyme C (DszC).
	gnl\|CDD\|32143	96	476	381	Gaps:66	80.66	393	26.18	15.14	5e-19	COG1960 CaiA Acyl-CoA dehydrogenases [Lipid metabolism].
	gnl\|CDD\|173840	127	323	197	Gaps:31	43.01	386	31.33	15.06	7e-11	cd01151 GCD Glutaryl-CoA dehydrogenase. Glutaryl-CoA dehydrogenase (GCD). GCD is an acyl-CoA dehydrogenase which catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and carbon dioxide in the catabolism of lysine hydroxylysine and tryptophan. It uses electron transfer flavoprotein (ETF) as an electron acceptor. GCD is a homotetramer. GCD deficiency leads to a severe neurological disorder in humans.
	gnl\|CDD\|173847	115	323	209	Gaps:29	48.79	373	31.87	13.74	2e-10	cd01158 SCAD_SBCAD Short chain acyl-CoA dehydrogenases and eukaryotic short/branched chain acyl-CoA dehydrogenases. Short chain acyl-CoA dehydrogenase (SCAD). SCAD is a mitochondrial beta-oxidation enzyme. It catalyzes the alpha beta dehydrogenation of the corresponding trans-enoyl-CoA by FAD which becomes reduced. The reduced form of SCAD is reoxidized in the oxidative half-reaction by electron-transferring flavoprotein (ETF) from which the electrons are transferred to the mitochondrial respiratory chain coupled with ATP synthesis. This subgroup also contains the eukaryotic short/branched chain acyl-CoA dehydrogenase(SBCAD) the bacterial butyryl-CoA dehydorgenase(BCAD) and 2-methylbutyryl-CoA dehydrogenase which is involved in isoleucine catabolism. These enzymes are homotetramers.
rpsblast_kog	gnl\|CDD\|35358	24	682	659	Gaps:41	95.82	670	40.97	19.00	1e-166	KOG0136 KOG0136 KOG0136 Acyl-CoA oxidase [Lipid transport and metabolism].
	gnl\|CDD\|35357	112	659	548	Gaps:54	80.18	661	36.42	16.23	1e-102	KOG0135 KOG0135 KOG0135 Pristanoyl-CoA/acyl-CoA oxidase [Lipid transport and metabolism Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|35360	114	323	210	Gaps:29	41.90	432	30.94	19.34	2e-13	KOG0138 KOG0138 KOG0138 Glutaryl-CoA dehydrogenase [Amino acid transport and metabolism].
	gnl\|CDD\|35362	31	323	293	Gaps:55	68.14	408	24.46	15.47	3e-09	KOG0140 KOG0140 KOG0140 Medium-chain acyl-CoA dehydrogenase [Lipid transport and metabolism].
	gnl\|CDD\|35361	107	326	220	Gaps:28	48.24	398	27.60	14.06	6e-09	KOG0139 KOG0139 KOG0139 Short-chain acyl-CoA dehydrogenase [Lipid transport and metabolism].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	YCKA_566070	411481	412266	728	100.00	100.00	n/a	Botrytis cinerea HortResearch libraries
	PD0ACA2YC24FM1	412214	412996	783	100.00	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA14YN09FM1	412307	413028	722	100.00	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA25YE23FM1	412738	413408	671	100.00	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA22YO20FM1	412452	413073	622	100.00	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA9YD13FM1	412166	412537	373	98.66	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA20YK23FM1	412876	413004	151	96.12	85.43	n/a	BT4 mycelium - pH stress library
	PD0ACA8YN12FM1	411295	412121	769	99.87	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA13YE21FM1	411297	412073	719	100.00	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA12YN17FM1	411297	412071	717	100.00	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA14YN03FM1	411291	412069	721	100.00	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA9YN12FM1	411295	412051	699	99.71	100.00	n/a	BT4 mycelium - pH stress library
sim4_Bot_allest4_clctg	CL_bt4_SuperContig_51_1_bt4ctg_0756_017.Contig1_v5	412166	413408	1243	99.84	100.00	n/a	all_lib_clone_nr:5
sim4_Bot_allest4_clctg	CL_bt4_SuperContig_51_1_bt4ctg_0756_016.Contig1_v5	411291	412121	773	99.87	100.00	n/a	all_lib_clone_nr:5
sim4_Ss_allest	no results
blastx_uniprot_sprot	O65202	411349	413404	664	39.75	96.99	n/a	Acyl-coenzyme A oxidase 1 peroxisomal - Arabidopsis thaliana (Mouse-ear cress)
	Q9ZQP2	411701	413449	664	42.25	84.49	n/a	Putative acyl-coenzyme A oxidase 1.2 peroxisomal - Arabidopsis thaliana (Mouse-ear cress)
	P07872	411698	413377	661	42.62	82.00	n/a	Acyl-coenzyme A oxidase 1 peroxisomal - Rattus norvegicus (Rat)
	Q8HYL8	411701	413377	661	43.07	81.85	n/a	Acyl-coenzyme A oxidase 1 peroxisomal - Phascolarctos cinereus (Koala)
	Q5RC19	411698	413377	660	42.80	82.12	n/a	Acyl-coenzyme A oxidase 1 peroxisomal - Pongo pygmaeus (Orangutan)
	Q15067	411698	413377	660	42.80	82.12	n/a	Acyl-coenzyme A oxidase 1 peroxisomal - Homo sapiens (Human)
	Q9R0H0	411698	413377	661	42.07	82.00	n/a	Acyl-coenzyme A oxidase 1 peroxisomal - Mus musculus (Mouse)
	Q9Z1N0	411698	413377	661	42.44	82.00	n/a	Acyl-coenzyme A oxidase 1 peroxisomal - Cavia porcellus (Guinea pig)
	Q3SZP5	411698	413377	660	41.88	82.12	n/a	Acyl-coenzyme A oxidase 1 peroxisomal - Bos taurus (Bovine)
	Q99424	411364	413377	681	35.98	90.60	n/a	Acyl-coenzyme A oxidase 2 peroxisomal - Homo sapiens (Human)
blastx_uniprot_trembl	Q4WGS8	412100	413425	444	73.29	98.65	n/a	Fatty-acyl coenzyme A oxidase - Aspergillus fumigatus (Sartorya fumigata)
	Q1DRG6	411304	413440	693	72.50	99.71	n/a	Putative uncharacterized protein - Coccidioides immitis
	Q2USC8	411310	413425	694	72.73	98.27	n/a	Acyl-CoA oxidase - Aspergillus oryzae
	A1DCV0	411310	413425	692	71.66	98.41	n/a	Acyl-CoA oxidase - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q0CJ26	411310	413425	693	70.53	98.41	n/a	Putative uncharacterized protein - Aspergillus terreus (strain NIH 2624)
	Q5AY78	411310	413425	696	70.42	98.13	n/a	Putative uncharacterized protein - Emericella nidulans (Aspergillus nidulans)
	A1CDU2	411310	413425	692	70.19	98.41	n/a	Acyl-CoA oxidase - Aspergillus clavatus
	Q4PCF5	411304	413377	688	50.76	96.22	n/a	Putative uncharacterized protein - Ustilago maydis (Smut fungus)
	Q1DRG6	411304	413440	693	72.50	99.71	n/a	Putative uncharacterized protein - Coccidioides immitis
	Q4PCF5	411304	413377	688	50.76	96.22	n/a	Putative uncharacterized protein - Ustilago maydis (Smut fungus)
blastx_FNG30_prot	SS1G_10238.1	411304	413446	735	93.80	94.42	n/a	sclerotinia_sclerotiorum hypothetical protein translation 736 aa
	gi:70986827:ref:XP_748901.1:	412100	413425	444	73.29	98.65	n/a	Aspergillus fumigatus Af293 palmitoyl-CoA oxidase 1
	CIMG_07097.2	411304	413440	693	72.50	99.71	n/a	Coccidioides immitis hypothetical protein translation 694 aa
	AO090005000479	411310	413425	694	72.73	98.27	n/a	Aspergillus oryzae acyl-CoA oxidase
	FG02287.1	411313	413428	696	71.78	98.28	n/a	fusarium_graminearum hypothetical protein 697 aa
	AN6752	411310	413425	696	70.42	98.13	n/a	Aspergillus nidulans AN6752.3 conserved hypothetical protein translation 697 aa
	SNU05259.1	411520	413431	663	70.59	92.31	n/a	Stagonospora nodorum hypothetical protein translation
	UM02208.1	411304	413377	688	50.76	96.22	n/a	ustilago_maydis hypothetical protein 689 aa
	RO3G_07433.1	411328	413407	699	49.02	94.85	n/a	rhizopus_oryzae hypothetical protein translation 700 aa
	RO3G_10134.1	411328	413404	695	48.19	95.54	n/a	rhizopus_oryzae hypothetical protein translation 696 aa
	SS1G_10238.1	411304	413446	735	93.80	94.42	n/a	sclerotinia_sclerotiorum hypothetical protein translation 736 aa
	CIMG_07097.2	411304	413440	693	72.50	99.71	n/a	Coccidioides immitis hypothetical protein translation 694 aa
	UM02208.1	411304	413377	688	50.76	96.22	n/a	ustilago_maydis hypothetical protein 689 aa
sim4_B0510_genes	BC1G_08482.1	411304	413458	2097	100.00	100.00	n/a	n/a
sim4_B0510_genes	BC1G_08482.1	411304	413458	2097	100.00	100.00	n/a	n/a
sim4_SS_genes	SS1G_10238.1	411304	413452	2208	86.44	91.21	n/a	n/a
	SS1G_10238.1	411304	413452	2208	86.44	91.21	n/a	n/a
	SS1G_10218.1	381067	477457	885	85.08	86.33	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.49	402704	415803	256765	99.73	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.15	411722	413458	1185750	86.53	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.15	411192	411637	1185750	83.41	n/a	n/a	n/a
blat_oligo	botrytisP00081503	412244	412303	60	n/a	n/a	n/a	array_gene:BofuT4_P058790_1 . max_over_threshold:147.54 . min_over_threshold:13.46 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081504	412609	412668	60	n/a	n/a	n/a	array_gene:BofuT4_P058790_1 . max_over_threshold:115.36 . min_over_threshold:7.65 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081505	412729	412788	60	n/a	n/a	n/a	array_gene:BofuT4_P058790_1 . max_over_threshold:113.34 . min_over_threshold:8.21 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081506	412849	412908	60	n/a	n/a	n/a	array_gene:BofuT4_P058790_1 . max_over_threshold:75.23 . min_over_threshold:5.23 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081507	413170	413229	60	n/a	n/a	n/a	array_gene:BofuT4_P058790_1 . max_over_threshold:103.93 . min_over_threshold:17.24 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081508	413332	413391	60	n/a	n/a	n/a	array_gene:BofuT4_P058790_1 . max_over_threshold:57.69 . min_over_threshold:7.51 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081510	413336	413395	60	n/a	n/a	n/a	array_gene:BofuT4_P058790_1 . max_over_threshold:65.2 . min_over_threshold:7.47 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081511	413340	413399	60	n/a	n/a	n/a	array_gene:BofuT4_P058790_1 . max_over_threshold:49.54 . min_over_threshold:5.35 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00081512	413422	413771	60	n/a	n/a	n/a	array_gene:BofuT4_P058790_1 . max_over_threshold:14.2 . min_over_threshold:0.77 . percent_hyb_over_threshold:96.42 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog