GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P054050.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	ssl:SS1G_05363	70	392	323	Gaps:22	90.94	331	77.74	7.64	1e-130	hypothetical protein
	aor:AO090005000147	2	639	638	Gaps:61	84.52	704	43.70	19.16	1e-126	2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases
	pno:SNOG_07872	3	639	637	Gaps:70	85.16	701	42.71	19.43	1e-122	hypothetical protein
	nfi:NFIA_007510	2	755	754	Gaps:101	95.74	705	40.44	18.96	1e-121	FAD binding domain protein
	bfu:BC1G_07100	1	466	466	Gaps:4	98.01	302	92.57	0.00	1e-121	hypothetical protein
	ani:AN3392.2	22	639	618	Gaps:62	80.72	721	42.10	20.27	1e-118	hypothetical protein
	pcs:Pc12g14040	2	639	638	Gaps:80	84.52	691	41.95	17.47	1e-108	Pc12g14040
	pno:SNOG_00952	2	561	560	Gaps:47	73.43	715	42.29	16.76	1e-104	hypothetical protein
	afm:AFUA_1G17670	2	755	754	Gaps:125	95.76	707	38.11	18.61	1e-100	salicylate hydroxylase (EC:1.14.13.1) K00480 salicylate hydroxylase [EC:1.14.13.1]
	fgr:FG04969.1	2	561	560	Gaps:45	74.08	706	41.68	17.40	1e-96	hypothetical protein
blastp_uniprot_sprot	sp\|P86491\|6HN3M_PSEFL	28	373	346	Gaps:38	83.12	385	30.31	17.81	1e-23	6-hydroxynicotinate 3-monooxygenase OS Pseudomonas fluorescens PE 1 SV 1
	sp\|P23262\|NHG1_PSEPU	140	398	259	Gaps:25	58.53	434	31.10	15.75	8e-19	Salicylate hydroxylase OS Pseudomonas putida GN nahG PE 1 SV 4
	sp\|Q53552\|NHG2_PSEPU	140	385	246	Gaps:31	56.32	435	31.84	15.10	7e-16	Salicylate hydroxylase OS Pseudomonas putida GN nahG PE 4 SV 1
	sp\|Q9F131\|3HBH1_PSEAC	28	374	347	Gaps:56	80.96	394	28.53	13.48	1e-15	3-hydroxybenzoate 6-hydroxylase 1 OS Pseudomonas alcaligenes GN xlnD PE 1 SV 1
	sp\|Q5EXK1\|3HBH_KLEOX	28	378	351	Gaps:48	81.36	397	27.24	17.34	1e-15	3-hydroxybenzoate 6-hydroxylase OS Klebsiella oxytoca GN mhbM PE 1 SV 1
blastp_pdb	2rgj_A	147	369	223	Gaps:44	51.49	402	28.99	19.81	6e-09	mol:protein length:402 Flavin-containing monooxygenase
blastp_pdb	3c96_A	147	369	223	Gaps:44	50.49	410	28.99	19.81	6e-09	mol:protein length:410 Flavin-containing monooxygenase
rpsblast_cdd	gnl\|CDD\|30999	1	400	400	Gaps:44	95.61	387	26.76	14.59	2e-41	COG0654 UbiH 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases [Coenzyme metabolism / Energy production and conversion].
	gnl\|CDD\|168569	1	365	365	Gaps:40	86.25	400	31.59	13.62	2e-41	PRK06475 PRK06475 salicylate hydroxylase Provisional.
	gnl\|CDD\|169249	1	378	378	Gaps:56	88.89	396	30.40	16.19	2e-40	PRK08163 PRK08163 salicylate hydroxylase Provisional.
	gnl\|CDD\|168661	3	378	376	Gaps:70	90.08	373	35.42	15.77	5e-37	PRK06753 PRK06753 hypothetical protein Provisional.
	gnl\|CDD\|168703	4	377	374	Gaps:71	91.47	375	31.49	14.58	1e-34	PRK06847 PRK06847 hypothetical protein Provisional.
	gnl\|CDD\|132263	3	385	383	Gaps:82	92.51	414	29.77	14.10	5e-34	TIGR03219 salicylate_mono salicylate 1-monooxygenase. Members of this protein family are salicylate 1-monooxygenase also called salicylate hydroxylase. This enzyme converts salicylate to catechol which is a common intermediate in the degradation of a number of aromatic compounds (phenol toluene benzoate etc.). The gene for this protein may occur in catechol degradation genes such as those of the meta-cleavage pathway.
	gnl\|CDD\|168999	3	379	377	Gaps:122	88.38	413	27.67	15.07	2e-29	PRK07538 PRK07538 hypothetical protein Provisional.
	gnl\|CDD\|162642	7	376	370	Gaps:62	89.35	385	25.00	16.57	2e-10	TIGR01988 Ubi-OHases Ubiquinone biosynthesis hydroxylase UbiH/UbiF/VisC/COQ6 family. This model represents a family of FAD-dependent hydroxylases (monooxygenases) which are all believed to act in the aerobic ubiquinone biosynthesis pathway. A separate set of hydroxylases as yet undiscovered are believed to be active under anaerobic conditions. In E. coli three enzyme activities have been described UbiB (which acts first at position 6 see TIGR01982) UbiH (which acts at position 4 ) and UbiF (which acts at position 5). UbiH and UbiF are similar to one another and form the basis of this subfamily. Interestingly E. coli contains another hydroxylase gene called visC that is highly similar to UbiF adjacent to UbiH and when mutated results in a phenotype similar to that of UbiH (which has also been named visB). Several other species appear to have three homologs in this family although they assort themselves differently on phylogenetic trees (e.g. Xylella and Mesorhizobium) making it difficult to ascribe a specific activity to each one. Eukaryotes appear to have only a single homolog in this subfamily (COQ6) which complements UbiH but also possess a non-orthologous gene COQ7 which complements UbiF.
	gnl\|CDD\|168922	299	362	64	Gaps:4	15.90	415	45.45	9.09	5e-07	PRK07364 PRK07364 2-octaprenyl-6-methoxyphenyl hydroxylase Validated.
rpsblast_kog	gnl\|CDD\|37825	4	371	368	Gaps:44	81.90	420	27.03	15.12	2e-21	KOG2614 KOG2614 KOG2614 Kynurenine 3-monooxygenase and related flavoprotein monooxygenases [Energy production and conversion General function prediction only].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	no results
sim4_Bot_allest4_clctg	no results
sim4_Ss_allest	no results
blastx_uniprot_sprot	no results
blastx_uniprot_trembl	A1D0Y2	215432	217085	705	53.12	59.01	n/a	FAD binding domain protein - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q0CBC2	215432	217085	704	54.07	57.53	n/a	Putative uncharacterized protein - Aspergillus terreus (strain NIH 2624)
	Q5B7T8	215553	216897	721	48.16	52.70	n/a	Putative uncharacterized protein - Emericella nidulans (Aspergillus nidulans)
	Q2UT58	215432	216560	704	50.30	46.59	n/a	RIB40 genomic DNA SC005 - Aspergillus oryzae
	Q0V4W2	215432	216527	715	49.85	45.73	n/a	Putative uncharacterized protein - Phaeosphaeria nodorum (Septoria nodorum)
	Q4WR37	215432	216446	707	50.34	41.87	n/a	Salicylate hydroxylase putative - Aspergillus fumigatus (Sartorya fumigata)
	Q2HI99	215532	216551	699	46.48	46.78	n/a	Putative uncharacterized protein - Chaetomium globosum (Soil fungus)
	Q0UK42	215432	217094	701	54.68	59.49	n/a	Putative uncharacterized protein - Phaeosphaeria nodorum (Septoria nodorum)
blastx_FNG30_prot	SS1G_05363.1	215643	216515	331	80.41	87.92	n/a	sclerotinia_sclerotiorum hypothetical protein translation 332 aa
	AN3392	215553	216897	710	48.16	53.52	n/a	Aspergillus nidulans AN3392.3 conserved hypothetical protein translation 711 aa
	AO090005000147	215432	216560	704	50.30	46.59	n/a	Aspergillus oryzae 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases
	SNU00952.1	215432	216527	715	49.85	45.73	n/a	Stagonospora nodorum hypothetical protein translation
	FG04969.1	215432	216548	706	48.14	45.61	n/a	fusarium_graminearum hypothetical protein 707 aa
	gi:70996756:ref:XP_753133.1:	215432	216446	707	50.34	41.87	n/a	Aspergillus fumigatus Af293 salicylate hydroxylase
	CHG00055.1	215532	216551	699	46.48	46.78	n/a	Chaetomium globosum hypothetical protein translation 700 aa
	AO090113000162	215541	216524	596	42.27	53.19	n/a	Aspergillus oryzae 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases
	AN6742	215670	216524	697	43.22	39.17	n/a	Aspergillus nidulans AN6742.3 conserved hypothetical protein translation 698 aa
	SNU07872.1	215432	217094	699	54.68	59.66	n/a	Stagonospora nodorum hypothetical protein translation
sim4_B0510_genes	BC1G_07100.1	215241	217088	909	90.21	100.00	n/a	n/a
sim4_SS_genes	SS1G_05363.1	215980	217088	996	81.99	53.51	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.36	213643	214845	299589	99.17	n/a	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.36	215232	220781	299589	99.86	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.6	216319	216531	2040374	82.63	n/a	n/a	n/a
	SS_supercontig_1.6	215436	215551	2040374	85.34	n/a	n/a	n/a
	SS_supercontig_1.6	216088	216233	2040374	82.88	n/a	n/a	n/a
	SS_supercontig_1.6	215639	215942	2040374	78.62	n/a	n/a	n/a
	SS_supercontig_1.6	215189	215264	2040374	88.16	n/a	n/a	n/a
blat_oligo	botrytisP00077773	215907	215966	60	n/a	n/a	n/a	array_gene:BofuT4_P054050_1 . max_over_threshold:68.05 . min_over_threshold:1.64 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00077774	215831	215890	60	n/a	n/a	n/a	array_gene:BofuT4_P054050_1 . max_over_threshold:43.95 . min_over_threshold:0.82 . percent_hyb_over_threshold:96.42 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00077775	215779	215838	60	n/a	n/a	n/a	array_gene:BofuT4_P054050_1 . max_over_threshold:19.14 . min_over_threshold:0.57 . percent_hyb_over_threshold:92.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00077776	215495	215554	60	n/a	n/a	n/a	array_gene:BofuT4_P054050_1 . max_over_threshold:21.4 . min_over_threshold:0.65 . percent_hyb_over_threshold:92.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00077778	215433	215490	60	n/a	n/a	n/a	array_gene:BofuT4_P054050_1 . max_over_threshold:47.47 . min_over_threshold:1.01 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00077779	215282	215341	60	n/a	n/a	n/a	array_gene:BofuT4_P054050_1 . max_over_threshold:52.55 . min_over_threshold:0.98 . percent_hyb_over_threshold:96.42 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00077780	215149	215208	60	n/a	n/a	n/a	array_gene:BofuT4_P054050_1 . max_over_threshold:81.75 . min_over_threshold:2.01 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00077781	214927	214986	60	n/a	n/a	n/a	array_gene:BofuT4_P054050_1 . max_over_threshold:84.11 . min_over_threshold:2.37 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00077782	214405	214464	60	n/a	n/a	n/a	array_gene:BofuT4_P054050_1 . max_over_threshold:55.86 . min_over_threshold:2.63 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog