GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P051850.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_06521	1	342	342	n/a	100.00	342	95.91	0.00	0.0	hypothetical protein
	ang:An12g01260	1	342	342	Gaps:22	100.00	320	80.31	8.75	1e-149	hypothetical protein K01501 nitrilase [EC:3.5.5.1]
	ssl:SS1G_10951	5	335	331	Gaps:60	38.40	987	60.16	10.29	1e-126	hypothetical protein
	aor:AO090003000470	4	341	338	Gaps:20	99.15	351	60.06	12.36	1e-115	carbon-nitrogen hydrolase K01501 nitrilase [EC:3.5.5.1]
	pcs:Pc22g01000	6	341	336	Gaps:31	98.89	361	59.66	10.64	1e-115	Pc22g01000
	afv:AFLA_033490	4	341	338	Gaps:20	99.15	351	59.77	12.64	1e-115	nitrilase
	ang:An06g01960	4	341	338	Gaps:24	99.15	355	59.09	11.36	1e-114	hypothetical protein K01501 nitrilase [EC:3.5.5.1]
	cim:CIMG_10233	5	341	337	Gaps:15	98.84	346	61.99	10.53	1e-114	hypothetical protein
	pcs:Pc22g07020	4	341	338	Gaps:21	99.14	350	59.94	12.97	1e-114	Pc22g07020
	act:ACLA_086750	7	341	335	Gaps:50	98.66	374	56.64	12.20	1e-114	hydrolase carbon-nitrogen family protein
blastp_uniprot_sprot	sp\|P46010\|NRL3_ARATH	43	333	291	Gaps:39	76.30	346	45.45	18.94	7e-56	Nitrilase 3 OS Arabidopsis thaliana GN NIT3 PE 1 SV 1
	sp\|P32961\|NRL1_ARATH	38	333	296	Gaps:39	77.75	346	44.61	18.22	8e-55	Nitrilase 1 OS Arabidopsis thaliana GN NIT1 PE 1 SV 2
	sp\|Q42966\|NRL4B_TOBAC	43	340	298	Gaps:38	77.59	348	42.22	20.00	1e-54	Bifunctional nitrilase/nitrile hydratase NIT4B OS Nicotiana tabacum GN NIT4B PE 2 SV 1
	sp\|P46011\|NRL4_ARATH	43	333	291	Gaps:39	74.37	355	43.56	19.32	2e-54	Bifunctional nitrilase/nitrile hydratase NIT4 OS Arabidopsis thaliana GN NIT4 PE 1 SV 1
	sp\|P32962\|NRL2_ARATH	38	333	296	Gaps:39	79.35	339	44.61	18.22	4e-54	Nitrilase 2 OS Arabidopsis thaliana GN NIT2 PE 1 SV 1
	sp\|Q42965\|NRL4A_TOBAC	43	340	298	Gaps:39	77.65	349	41.70	19.93	9e-54	Bifunctional nitrilase/nitrile hydratase NIT4A OS Nicotiana tabacum GN NIT4A PE 2 SV 1
	sp\|Q6H849\|NRL4_ORYSJ	43	333	291	Gaps:43	74.03	362	42.91	19.03	1e-52	Bifunctional nitrilase/nitrile hydratase NIT4 OS Oryza sativa subsp. japonica GN NIT4 PE 2 SV 1
	sp\|P40447\|NIT1_YEAST	33	229	197	Gaps:29	84.42	199	47.02	20.83	3e-34	Putative nitrilase-like protein NIT1 OS Saccharomyces cerevisiae GN NIT1 PE 5 SV 1
	sp\|P20960\|NRLA_ALCFA	42	333	292	Gaps:48	74.72	356	31.58	19.92	2e-22	Nitrilase arylacetone-specific OS Alcaligenes faecalis PE 1 SV 2
	sp\|Q02068\|NRL1_RHORH	42	340	299	Gaps:48	71.80	383	29.09	19.64	2e-22	Aliphatic nitrilase OS Rhodococcus rhodochrous PE 1 SV 1
blastp_pdb	no results
rpsblast_cdd	gnl\|CDD\|143588	20	333	314	Gaps:47	94.61	297	45.55	16.73	2e-85	cd07564 nitrilases_CHs Nitrilases cyanide hydratase (CH)s and similar proteins (class 1 nitrilases). Nitrilases (nitrile aminohydrolases EC:3.5.5.1) hydrolyze nitriles (RCN) to ammonia and the corresponding carboxylic acid. Most nitrilases prefer aromatic nitriles some prefer arylacetonitriles and others aliphatic nitriles. This group includes the nitrilase cyanide dihydratase (CDH) which hydrolyzes inorganic cyanide (HCN) to produce formate. It also includes cyanide hydratase (CH) which hydrolyzes HCN to formamide. This group includes four Arabidopsis thaliana nitrilases (Ath)NIT1-4. AthNIT1-3 have a strong substrate preference for phenylpropionitrile (PPN) and other nitriles which may originate from the breakdown of glucosinolates. The product of PPN hydrolysis phenylacetic acid has auxin activity. AthNIT1-3 can also convert indoacetonitrile to indole-3-acetic acid (IAA auxin) but with a lower affinity and velocity. From their expression patterns it has been speculated that NIT3 may produce IAA during the early stages of germination and that NIT3 may produce IAA during embryo development and maturation. AthNIT4 has a strong substrate specificity for the nitrile beta-cyano-L-alanine (Ala(CN)) an intermediate of cyanide detoxification. AthNIT4 has both a nitrilase activity and a nitrile hydratase (NHase) activity which generate aspartic acid and asparagine respectively from Ala(CN). NHase catalyzes the hydration of nitriles to their corresponding amides. This subgroup belongs to a larger nitrilase superfamily comprised of belong to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) this subgroup corresponds to class 1.
	gnl\|CDD\|166145	2	333	332	Gaps:42	88.63	343	44.08	14.80	2e-68	PLN02504 PLN02504 nitrilase.
	gnl\|CDD\|143587	9	314	306	Gaps:77	96.05	253	31.28	18.93	1e-25	cd07197 nitrilase Nitrilase superfamily including nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes. This superfamily (also known as the C-N hydrolase superfamily) contains hydrolases that break carbon-nitrogen bonds it includes nitrilases cyanide dihydratases aliphatic amidases N-terminal amidases beta-ureidopropionases biotinidases pantotheinase N-carbamyl-D-amino acid amidohydrolases the glutaminase domain of glutamine-dependent NAD+ synthetase apolipoprotein N-acyltransferases and N-carbamoylputrescine amidohydrolases among others. These enzymes depend on a Glu-Lys-Cys catalytic triad and work through a thiol acylenzyme intermediate. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer. These oligomers include dimers tetramers hexamers octamers tetradecamers octadecamers as well as variable length helical arrangements and homo-oligomeric spirals. These proteins have roles in vitamin and co-enzyme metabolism in detoxifying small molecules in the synthesis of signaling molecules and in the post-translational modification of proteins. They are used industrially as biocatalysts in the fine chemical and pharmaceutical industry in cyanide remediation and in the treatment of toxic effluent. This superfamily has been classified previously in the literature based on global and structure-based sequence analysis into thirteen different enzyme classes (referred to as 1-13). This hierarchy includes those thirteen classes and a few additional subfamilies. A putative distant relative the plasmid-borne TraB family has not been included in the hierarchy.
	gnl\|CDD\|30737	5	333	329	Gaps:69	99.27	274	28.68	13.97	3e-19	COG0388 COG0388 Predicted amidohydrolase [General function prediction only].
	gnl\|CDD\|144405	8	226	219	Gaps:57	100.00	172	31.40	14.53	2e-18	pfam00795 CN_hydrolase Carbon-nitrogen hydrolase. This family contains hydrolases that break carbon-nitrogen bonds. The family includes: Nitrilase EC:3.5.5.1 Aliphatic amidase EC:3.5.1.4 Biotidinase EC:3.5.1.12 Beta-ureidopropionase EC:3.5.1.6. Nitrilase-related proteins generally have a conserved E-K-C catalytic triad and are multimeric alpha-beta-beta-alpha sandwich proteins.
	gnl\|CDD\|143600	8	318	311	Gaps:77	96.85	254	30.08	15.45	1e-14	cd07576 R-amidase_like Pseudomonas sp. MCI3434 R-amidase and related proteins (putative class 13 nitrilases). Pseudomonas sp. MCI3434 R-amidase hydrolyzes (R S)-piperazine-2-tert-butylcarboxamide to form (R)-piperazine-2-carboxylic acid. It does so with strict R-stereoselectively. Its preferred substrates are carboxamide compounds which have the amino or imino group connected to their beta- or gamma-carbon. This subgroup belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) class 13 represents proteins that at the time were difficult to place in a distinct similarity group. It has been suggested that this subgroup represents a new class. Members of the nitrilase superfamily generally form homomeric complexes the basic building block of which is a homodimer. Native R-amidase however appears to be a monomer.
	gnl\|CDD\|143604	124	316	193	Gaps:29	67.91	268	30.22	14.29	8e-13	cd07580 nitrilase_2 Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases). The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
	gnl\|CDD\|143601	114	330	217	Gaps:27	74.90	259	31.96	14.95	1e-12	cd07577 Ph0642_like Pyrococcus horikoshii Ph0642 and related proteins members of the nitrilase superfamily (putative class 13 nitrilases). Uncharacterized subgroup of the nitrilase superfamily. This superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. Pyrococcus horikoshii Ph0642 is a hypothetical protein belonging to this subgroup. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). This subgroup was classified as belonging to class 13 which represents proteins that at the time were difficult to place in a distinct similarity group. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
	gnl\|CDD\|143610	117	317	201	Gaps:42	72.49	269	29.74	17.44	3e-12	cd07586 nitrilase_8 Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases). The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
	gnl\|CDD\|143607	112	309	198	Gaps:34	71.94	253	30.77	15.93	6e-11	cd07583 nitrilase_5 Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases). The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
rpsblast_kog	gnl\|CDD\|36023	10	333	324	Gaps:41	87.54	337	44.07	17.97	1e-74	KOG0805 KOG0805 KOG0805 Carbon-nitrogen hydrolase [Amino acid transport and metabolism].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	B2BC_045_112_B06	96791	96996	323	98.06	63.78	n/a	B0510 Spores suspension on bean library
	BcA1_3759_C14	96280	97179	724	98.48	100.00	n/a	BC A1 mycelium
	AL116866	96330	96936	483	100.00	100.00	n/a	BT4 Mycelium - nitrate starvation library
	AL115465	96298	96936	515	100.00	100.00	n/a	BT4 Mycelium - nitrate starvation library
	PD0ACA2YJ01FM1	96000	96846	724	99.86	100.00	n/a	BT4 mycelium - pH stress library
	YCHA_535056	95946	96645	576	98.61	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCHA_538272	95947	96678	609	98.52	100.00	n/a	Botrytis cinerea HortResearch libraries
	B1BC_045_017_D06	95953	96657	581	91.05	100.00	n/a	BC ATCC 58025 (G3)
	B1BC_045_017_D05	95953	96689	611	86.58	100.00	n/a	BC ATCC 58025 (G3)
	B1BC_045_017_E11	95953	96654	580	90.69	100.00	n/a	BC ATCC 58025 (G3)
	PD0ABA6YL13FM1	95958	96702	621	84.54	100.00	n/a	BT4 young mycelium library
	PD0ABA4YA17FM1	95964	96659	572	99.65	100.00	n/a	BT4 young mycelium library
	PD0ACA13YC09FM1	95822	96651	706	100.00	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA18YD08FM1	95933	96813	757	100.00	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA12YL17FM1	95933	96688	632	100.00	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA9YP05FM1	95936	96625	566	99.65	100.00	n/a	BT4 mycelium - pH stress library
	PD0ACA1YK08FM1	95949	96313	367	98.63	99.46	n/a	BT4 mycelium - pH stress library
	PD0ABA6YL13NM1r	96278	97281	829	99.88	100.00	n/a	BT4 young mycelium library
	PD0ABA4YA17NM1r	96275	97280	830	100.00	100.00	n/a	BT4 young mycelium library
sim4_Bot_allest4_clctg	CL_bt4_SuperContig_48_1_bt4ctg_0684_009.Contig1_v5	95822	97280	1283	100.00	100.00	n/a	all_lib_clone_nr:11
sim4_Ss_allest	no results
blastx_uniprot_sprot	P46010	96339	97101	346	44.39	61.85	n/a	Nitrilase 3 - Arabidopsis thaliana (Mouse-ear cress)
	P32962	96607	97083	339	46.25	47.20	n/a	Nitrilase 2 - Arabidopsis thaliana (Mouse-ear cress)
	P32961	96607	97083	346	45.62	46.24	n/a	Nitrilase 1 - Arabidopsis thaliana (Mouse-ear cress)
	P46011	96607	97101	355	43.98	46.76	n/a	Nitrilase 4 - Arabidopsis thaliana (Mouse-ear cress)
	Q42965	96607	97083	349	44.38	45.85	n/a	Nitrilase 4 - Nicotiana tabacum (Common tobacco)
blastx_uniprot_trembl	A2QYH5	95985	97104	320	80.67	93.75	n/a	Catalytic activity: Nitrile + H2O Carboxylate + NH3. precursor - Aspergillus niger
	Q1DHI0	95997	97183	346	63.25	95.95	n/a	Putative uncharacterized protein - Coccidioides immitis
	A4R7D5	95997	97180	324	61.27	87.65	n/a	Putative uncharacterized protein - Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea)
	Q5AWG3	96003	97183	347	63.55	95.68	n/a	Putative uncharacterized protein - Emericella nidulans (Aspergillus nidulans)
	Q2ULB8	95994	97183	351	61.54	96.30	n/a	Carbon-nitrogen hydrolase - Aspergillus oryzae
	Q877A7	95994	97183	351	61.24	96.30	n/a	Hypothetical nitrilase-like protein - Aspergillus oryzae
	A2QLP4	95994	97183	355	60.82	96.34	n/a	Catalytic activity: a nitrile + H(2)O < > a carboxylate + NH(3). precursor - Aspergillus niger
	A1CUI7	96003	97183	374	58.50	95.99	n/a	Hydrolase carbon-nitrogen family protein - Aspergillus clavatus
	A2QYH5	95985	97104	320	80.67	93.75	n/a	Catalytic activity: Nitrile + H2O Carboxylate + NH3. precursor - Aspergillus niger
blastx_FNG30_prot	CIMG_10233.2	95997	97183	346	63.25	95.95	n/a	Coccidioides immitis hypothetical protein translation 347 aa
	MGG_03280.5	95997	97180	324	61.27	87.65	n/a	magnaporthe_grisea hypothetical protein translation 325 aa
	AN7367	96003	97183	347	63.55	95.68	n/a	Aspergillus nidulans AN7367.3 conserved hypothetical protein translation 348 aa
	AO090003000470	95994	97183	351	61.54	96.30	n/a	Aspergillus oryzae carbon-nitrogen hydrolase
	jgi:Triree:16177	96003	97180	342	58.48	96.49	n/a	Trichoderma reesei
	SNU02380.1	95994	97183	421	64.89	84.56	n/a	Stagonospora nodorum hypothetical protein translation
	gi:70992745:ref:XP_751221.1:	95994	96897	419	64.08	58.47	n/a	Aspergillus fumigatus Af293 nitrilase
	NCU05387.2	95991	97180	476	58.08	76.68	n/a	Neurospora crassa hypothetical protein AL451011 conserved hypothetical protein [Neurospora crassa] translation 477 aa
	CHG10511.1	96000	96945	439	53.73	61.05	n/a	Chaetomium globosum hypothetical protein translation 440 aa
	SS1G_10951.1	95964	96918	987	73.18	26.44	n/a	sclerotinia_sclerotiorum hypothetical protein translation 988 aa
sim4_B0510_genes	BC1G_06521.1	95985	97189	1029	99.32	100.00	n/a	n/a
sim4_SS_genes	no results
blastn_b0510_supercontig	B0510_supercontig_1.32	95983	101807	331639	99.12	n/a	n/a	n/a
blastn_SS_supercontig	no results
blat_oligo	botrytisP00075933	96078	96137	60	n/a	n/a	n/a	array_gene:BofuT4_P051850_1 . max_over_threshold:31.69 . min_over_threshold:1.25 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00075934	96224	96283	60	n/a	n/a	n/a	array_gene:BofuT4_P051850_1 . max_over_threshold:120.79 . min_over_threshold:15.1 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:1
	botrytisP00075935	96749	96808	60	n/a	n/a	n/a	array_gene:BofuT4_P051850_1 . max_over_threshold:145.02 . min_over_threshold:23.16 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00075936	96757	96816	60	n/a	n/a	n/a	array_gene:BofuT4_P051850_1 . max_over_threshold:124.46 . min_over_threshold:15.15 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00075938	96761	96820	60	n/a	n/a	n/a	array_gene:BofuT4_P051850_1 . max_over_threshold:98 . min_over_threshold:10.14 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00075939	96765	96824	60	n/a	n/a	n/a	array_gene:BofuT4_P051850_1 . max_over_threshold:119.27 . min_over_threshold:14.45 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00075940	96790	96849	60	n/a	n/a	n/a	array_gene:BofuT4_P051850_1 . max_over_threshold:75.04 . min_over_threshold:7.93 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00075941	96812	96871	60	n/a	n/a	n/a	array_gene:BofuT4_P051850_1 . max_over_threshold:89.62 . min_over_threshold:6.17 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00075942	97133	97189	60	n/a	n/a	n/a	array_gene:BofuT4_P051850_1 . max_over_threshold:93.43 . min_over_threshold:15.36 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog