GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P027100.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	ssl:SS1G_12939	15	590	576	Gaps:1	95.85	602	81.98	5.72	0.0	hypothetical protein
	bfu:BC1G_11888	14	596	583	Gaps:19	57.29	761	99.54	0.00	1e-162	hypothetical protein
	ang:An11g05580	18	593	576	Gaps:18	97.96	588	50.87	12.85	1e-150	hypothetical protein
	aor:AO090103000214	32	593	562	Gaps:18	95.25	590	50.89	12.99	1e-147	choline dehydrogenase and related flavoproteins
	afv:AFLA_076820	32	591	560	Gaps:17	94.60	593	50.09	14.80	1e-146	glucose oxidase putative
	aor:AO090005000449	32	591	560	Gaps:22	94.56	588	50.18	15.11	1e-144	choline dehydrogenase and related flavoproteins
	ang:An07g00450	32	590	559	Gaps:17	93.96	596	47.68	16.07	1e-139	hypothetical protein
	ssl:SS1G_14293	30	591	562	Gaps:30	96.10	589	48.23	13.07	1e-131	hypothetical protein
	nfi:NFIA_034090	33	589	557	Gaps:18	93.61	595	46.50	16.70	1e-130	GMC oxidoreductase
	act:ACLA_093090	28	589	562	Gaps:27	97.20	571	45.41	16.76	1e-127	GMC oxidoreductase
blastp_uniprot_sprot	sp\|P81156\|GOX_PENAG	32	593	562	Gaps:47	95.23	587	35.06	16.10	2e-67	Glucose oxidase OS Penicillium amagasakiense PE 1 SV 1
	sp\|P13006\|GOX_ASPNG	33	593	561	Gaps:44	92.40	605	34.88	13.42	8e-66	Glucose oxidase OS Aspergillus niger GN gox PE 1 SV 1
	sp\|Q92452\|GOX_TALFL	32	593	562	Gaps:47	92.40	605	34.88	16.28	7e-65	Glucose oxidase OS Talaromyces flavus GN GOX PE 3 SV 1
	sp\|Q8NUM0\|BETA_STAAW	31	590	560	Gaps:63	93.32	569	33.15	15.82	2e-53	Choline dehydrogenase OS Staphylococcus aureus (strain MW2) GN betA PE 3 SV 1
	sp\|Q6G664\|BETA_STAAS	31	590	560	Gaps:63	93.32	569	33.15	15.82	2e-53	Choline dehydrogenase OS Staphylococcus aureus (strain MSSA476) GN betA PE 3 SV 1
	sp\|B4EX94\|BETA_PROMH	30	590	561	Gaps:69	95.86	555	32.14	17.48	2e-53	Choline dehydrogenase OS Proteus mirabilis (strain HI4320) GN betA PE 3 SV 1
	sp\|A8Z5A4\|BETA_STAAT	31	590	560	Gaps:63	93.32	569	32.96	15.82	1e-52	Choline dehydrogenase OS Staphylococcus aureus (strain USA300 / TCH1516) GN betA PE 3 SV 1
	sp\|Q6GDJ1\|BETA_STAAR	31	590	560	Gaps:63	93.32	569	32.96	15.82	1e-52	Choline dehydrogenase OS Staphylococcus aureus (strain MRSA252) GN betA PE 3 SV 1
	sp\|P60337\|BETA_STAAN	31	590	560	Gaps:63	93.32	569	32.96	15.82	1e-52	Choline dehydrogenase OS Staphylococcus aureus (strain N315) GN betA PE 1 SV 1
	sp\|P60336\|BETA_STAAM	31	590	560	Gaps:63	93.32	569	32.96	15.82	1e-52	Choline dehydrogenase OS Staphylococcus aureus (strain Mu50 / ATCC 700699) GN betA PE 1 SV 1
blastp_pdb	1gpe_B	32	593	562	Gaps:47	95.23	587	35.06	16.10	4e-68	mol:protein length:587 PROTEIN (GLUCOSE OXIDASE)
	1gpe_A	32	593	562	Gaps:47	95.23	587	35.06	16.10	4e-68	mol:protein length:587 PROTEIN (GLUCOSE OXIDASE)
	1gal_A	33	593	561	Gaps:44	95.88	583	34.88	13.42	1e-66	mol:protein length:583 GLUCOSE OXIDASE
	1cf3_A	33	593	561	Gaps:44	95.88	583	34.88	13.42	1e-66	mol:protein length:583 PROTEIN (GLUCOSE OXIDASE)
	3fim_B	32	590	559	Gaps:58	99.12	566	29.95	19.25	1e-52	mol:protein length:566 Aryl-alcohol oxidase
	3ljp_B	32	590	559	Gaps:71	94.14	546	28.79	18.29	2e-38	mol:protein length:546 Choline oxidase
	3ljp_A	32	590	559	Gaps:71	94.14	546	28.79	18.29	2e-38	mol:protein length:546 Choline oxidase
	3nne_H	32	590	559	Gaps:71	94.14	546	28.79	18.29	2e-38	mol:protein length:546 Choline oxidase
	3nne_G	32	590	559	Gaps:71	94.14	546	28.79	18.29	2e-38	mol:protein length:546 Choline oxidase
	3nne_F	32	590	559	Gaps:71	94.14	546	28.79	18.29	2e-38	mol:protein length:546 Choline oxidase
rpsblast_cdd	gnl\|CDD\|32458	25	591	567	Gaps:50	98.71	542	32.15	14.95	4e-70	COG2303 BetA Choline dehydrogenase and related flavoproteins [Amino acid transport and metabolism].
	gnl\|CDD\|162541	33	590	558	Gaps:53	99.06	532	33.78	15.37	4e-68	TIGR01810 betA choline dehydrogenase. This enzyme is a member of the GMC oxidoreductase family (pfam00732 and pfam05199) sharing a common evoluntionary origin and enzymatic reaction with alcohol dehydrogenase. Outgrouping from this model Caulobacter crescentus shares sequence homology with choline dehydrogenase yet other genes participating in this enzymatic reaction have not currently been identified.
	gnl\|CDD\|167312	32	589	558	Gaps:58	79.05	549	38.25	16.13	1e-58	PRK02106 PRK02106 choline dehydrogenase Validated.
	gnl\|CDD\|147408	445	585	141	Gaps:8	100.00	139	41.73	17.27	7e-35	pfam05199 GMC_oxred_C GMC oxidoreductase. This domain found associated with pfam00732.
	gnl\|CDD\|109776	99	344	246	Gaps:52	93.58	218	34.31	13.24	4e-27	pfam00732 GMC_oxred_N GMC oxidoreductase. This family of proteins bind FAD as a cofactor.
	gnl\|CDD\|166426	31	347	317	Gaps:74	47.92	578	32.13	18.77	8e-13	PLN02785 PLN02785 aldehyde-lyase/mandelonitrile lyase.
rpsblast_kog	gnl\|CDD\|36452	30	595	566	Gaps:73	90.37	623	31.26	17.05	2e-79	KOG1238 KOG1238 KOG1238 Glucose dehydrogenase/choline dehydrogenase/mandelonitrile lyase (GMC oxidoreductase family) [General function prediction only].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	PD0ACA7YO13FM1	93250	94014	711	100.00	100.00	n/a	BT4 mycelium - pH stress library
	YCGA_540907	93606	94238	579	99.65	100.00	n/a	Botrytis cinerea HortResearch libraries
	PD0ABA16YD20FM1	72324	124990	680	98.95	97.94	n/a	BT4 young mycelium library
sim4_Bot_allest4_clctg	PD0ACA7YO13FM1	93250	94014	711	100.00	100.00	n/a	BT4 mycelium - pH stress library
sim4_Ss_allest	G787P560RH3.T0	58832	107985	850	86.05	85.18	n/a	Ss ESTs grown as developing apothecia
	G787P523RE8.T0	58836	107985	796	86.14	96.98	n/a	Ss ESTs grown as developing apothecia
	G787P5114RH11.T0	58913	107985	775	86.21	89.81	n/a	Ss ESTs grown as developing apothecia
blastx_uniprot_sprot	P81156	92149	94020	587	34.30	93.87	n/a	Glucose oxidase - Penicillium amagasakiense
	Q92452	92149	94020	605	34.12	91.07	n/a	Glucose oxidase precursor - Talaromyces flavus
	P18173	92574	94026	625	36.79	67.84	n/a	Glucose dehydrogenase [acceptor] precursor - Drosophila melanogaster (Fruit fly)
	P18172	92574	94026	625	36.32	67.84	n/a	Glucose dehydrogenase [acceptor] precursor - Drosophila pseudoobscura (Fruit fly)
	P13006	93109	94017	605	35.21	46.94	n/a	Glucose oxidase precursor - Aspergillus niger
	A7N2P9	92961	94020	569	38.13	52.55	n/a	Choline dehydrogenase - Vibrio harveyi (strain ATCC BAA-1116 / BB120)
	A6T613	92958	94020	554	36.07	55.05	n/a	Choline dehydrogenase - Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
	Q8PPG8	92442	94020	556	32.82	81.12	n/a	Choline dehydrogenase - Xanthomonas axonopodis pv. citri
	Q88CW6	92469	94020	565	31.59	81.24	n/a	Choline dehydrogenase - Pseudomonas putida (strain KT2440)
	Q3BXK8	92529	94020	556	32.94	76.44	n/a	Choline dehydrogenase - Xanthomonas campestris pv. vesicatoria (strain 85-10)
blastx_uniprot_trembl	A2QWL1	92149	94062	588	51.75	97.28	n/a	Contig An11c0220 complete genome. precursor - Aspergillus niger
	Q0CDD9	92158	94038	591	49.64	94.75	n/a	Putative uncharacterized protein - Aspergillus terreus (strain NIH 2624)
	Q2TYK0	92149	94020	590	51.88	94.75	n/a	Choline dehydrogenase and related flavoproteins - Aspergillus oryzae
	Q2USF2	92155	94020	588	50.99	94.05	n/a	Choline dehydrogenase and related flavoproteins - Aspergillus oryzae
	A2QM15	92158	94020	596	48.29	93.12	n/a	Catalytic activity: beta-D-glucose + O2 D-glucono-1 precursor - Aspergillus niger
	Q0CFL8	92433	94044	527	50.10	95.83	n/a	Predicted protein - Aspergillus terreus (strain NIH 2624)
	Q4X037	92155	94164	636	44.23	98.11	n/a	Glucose oxidase putative - Aspergillus fumigatus (Sartorya fumigata)
blastx_FNG30_prot	SS1G_12939.1	92158	94113	602	83.16	97.67	n/a	sclerotinia_sclerotiorum hypothetical protein translation 603 aa
	AO090103000214	92149	94020	590	51.88	94.75	n/a	Aspergillus oryzae choline dehydrogenase and related flavoproteins
	AO090005000449	92155	94020	588	50.99	94.05	n/a	Aspergillus oryzae choline dehydrogenase and related flavoproteins
	SS1G_14293.1	92155	94089	589	47.60	99.15	n/a	sclerotinia_sclerotiorum hypothetical protein translation 590 aa
	jgi:Triree:30736	92161	94023	594	47.58	93.77	n/a	Trichoderma reesei
	gi:70988923:ref:XP_749312.1:	92161	94017	599	46.82	91.99	n/a	Aspergillus fumigatus Af293 GMC oxidoreductase
	SNU11103.1	92167	94020	587	46.87	95.23	n/a	Stagonospora nodorum hypothetical protein translation
	MGG_07580.5	92158	94065	577	44.40	96.01	n/a	magnaporthe_grisea hypothetical protein translation 578 aa
	MGG_07941.5	92155	93864	542	41.73	95.94	n/a	magnaporthe_grisea hypothetical protein translation 543 aa
	gi:71002270:ref:XP_755816.1:	92155	94164	636	44.23	98.11	n/a	Aspergillus fumigatus Af293 glucose oxidase
sim4_B0510_genes	BC1G_11888.1	92137	95714	2286	99.87	100.00	n/a	n/a
sim4_SS_genes	SS1G_12939.1	92159	94032	1809	84.00	70.48	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.95	89819	92726	169463	99.90	n/a	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.95	92788	104285	169463	99.93	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.22	93107	93656	755171	81.82	n/a	n/a	n/a
	SS_supercontig_1.22	92737	93063	755171	81.35	n/a	n/a	n/a
	SS_supercontig_1.22	92159	92346	755171	84.57	n/a	n/a	n/a
	SS_supercontig_1.22	93753	94017	755171	81.13	n/a	n/a	n/a
blat_oligo	botrytisP00057268	93673	93786	60	n/a	n/a	n/a	array_gene:BofuT4_P027100_1 . max_over_threshold:25.54 . min_over_threshold:0.47 . percent_hyb_over_threshold:78.57 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00057269	93344	93403	60	n/a	n/a	n/a	array_gene:BofuT4_P027100_1 . max_over_threshold:48.18 . min_over_threshold:0.7 . percent_hyb_over_threshold:89.28 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00057271	93044	93152	60	n/a	n/a	n/a	array_gene:BofuT4_P027100_1 . max_over_threshold:112.02 . min_over_threshold:0.92 . percent_hyb_over_threshold:96.42 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00057272	92962	93021	60	n/a	n/a	n/a	array_gene:BofuT4_P027100_1 . max_over_threshold:100.86 . min_over_threshold:0.84 . percent_hyb_over_threshold:92.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00057273	92906	92965	60	n/a	n/a	n/a	array_gene:BofuT4_P027100_1 . max_over_threshold:79.36 . min_over_threshold:1.2 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00057274	92789	92848	60	n/a	n/a	n/a	array_gene:BofuT4_P027100_1 . max_over_threshold:69.9 . min_over_threshold:0.94 . percent_hyb_over_threshold:96.42 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00057275	92289	92348	60	n/a	n/a	n/a	array_gene:BofuT4_P027100_1 . max_over_threshold:38.14 . min_over_threshold:0.59 . percent_hyb_over_threshold:82.14 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00057276	92223	92282	60	n/a	n/a	n/a	array_gene:BofuT4_P027100_1 . max_over_threshold:44.14 . min_over_threshold:0.69 . percent_hyb_over_threshold:85.71 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00057277	92173	92232	60	n/a	n/a	n/a	array_gene:BofuT4_P027100_1 . max_over_threshold:50.32 . min_over_threshold:0.68 . percent_hyb_over_threshold:89.28 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog