GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P024960.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	ssl:SS1G_14206	31	613	583	n/a	94.80	615	93.83	3.77	0.0	similar to FreB
	fgr:FG08525.1	32	599	568	Gaps:12	53.64	1070	56.45	16.72	0.0	hypothetical protein
	pan:PODANSg4461	34	613	580	Gaps:15	92.97	640	54.29	15.80	1e-180	hypothetical protein
	ncr:NCU02009	87	613	527	Gaps:24	100.00	549	57.56	17.30	1e-178	similar to FreB
	ang:An01g12390	38	613	576	Gaps:21	96.45	619	47.57	19.26	1e-157	hypothetical protein
	act:ACLA_021070	38	613	576	Gaps:25	96.46	621	46.41	19.53	1e-155	metalloreductase putative
	afm:AFUA_1G14340	38	613	576	Gaps:22	96.45	620	46.82	19.40	1e-154	metalloreductase (EC:1.16.1.-) K05920 [EC:1.16.1.-]
	afv:AFLA_042050	38	613	576	Gaps:31	96.49	627	47.60	18.84	1e-154	metalloreductase putative
	nfi:NFIA_011070	38	613	576	Gaps:22	96.45	620	45.65	21.07	1e-152	metalloreductase putative
	aor:AO090003000630	44	613	570	Gaps:8	95.61	592	47.70	18.02	1e-151	ferric reductase NADH/NADPH oxidase and related proteins
blastp_uniprot_sprot	sp\|Q08905\|FRE3_YEAST	107	613	507	Gaps:78	71.31	711	26.23	21.10	3e-35	Ferric reductase transmembrane component 3 OS Saccharomyces cerevisiae GN FRE3 PE 1 SV 1
	sp\|P36033\|FRE2_YEAST	115	613	499	Gaps:77	70.32	711	24.60	21.00	4e-32	Ferric/cupric reductase transmembrane component 2 OS Saccharomyces cerevisiae GN FRE2 PE 1 SV 1
	sp\|P53746\|FRE4_YEAST	115	613	499	Gaps:106	71.63	719	23.30	22.52	2e-28	Ferric reductase transmembrane component 4 OS Saccharomyces cerevisiae GN FRE4 PE 1 SV 1
	sp\|P32791\|FRE1_YEAST	107	613	507	Gaps:64	73.62	686	25.15	19.21	3e-27	Ferric/cupric reductase transmembrane component 1 OS Saccharomyces cerevisiae GN FRE1 PE 1 SV 1
	sp\|P78588\|FREL_CANAL	38	613	576	Gaps:113	83.86	669	26.02	18.89	2e-26	Probable ferric reductase transmembrane component OS Candida albicans GN CFL1 PE 3 SV 1
	sp\|Q08908\|FRE5_YEAST	121	613	493	Gaps:63	69.45	694	24.07	20.95	8e-25	Ferric reductase transmembrane component 5 OS Saccharomyces cerevisiae GN FRE5 PE 2 SV 1
	sp\|Q12473\|FRE6_YEAST	46	613	568	Gaps:94	77.25	712	25.27	20.73	1e-24	Ferric reductase transmembrane component 6 OS Saccharomyces cerevisiae GN FRE6 PE 1 SV 1
	sp\|A6ZN61\|FRE7_YEAS7	173	525	353	Gaps:59	52.26	620	27.16	19.44	2e-16	Ferric/cupric reductase transmembrane component 7 OS Saccharomyces cerevisiae (strain YJM789) GN FRE7 PE 2 SV 1
	sp\|Q12333\|FRE7_YEAST	173	518	346	Gaps:56	51.61	620	26.88	19.38	3e-16	Ferric/cupric reductase transmembrane component 7 OS Saccharomyces cerevisiae GN FRE7 PE 1 SV 2
	sp\|O94727\|FRP2_SCHPO	175	597	423	Gaps:63	77.66	564	23.06	18.04	6e-15	Ferric/cupric reductase transmembrane component 2 OS Schizosaccharomyces pombe GN SPBC947.05c PE 2 SV 1
blastp_pdb	no results
rpsblast_cdd	gnl\|CDD\|99783	343	611	269	Gaps:65	100.00	210	39.05	18.10	1e-44	cd06186 NOX_Duox_like_FAD_NADP NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes but are now also implicated in cell signaling and metabolism. NOX has a 6-alpha helix heme-binding transmembrane domain fused to a flavoprotein with the nucleotide binding domain located in the cytoplasm. Duox enzymes link a peroxidase domain to the NOX domain via a single transmembrane and EF-hand Ca2+ binding sites. The flavoprotein module has a ferredoxin like FAD/NADPH binding domain. In classical phagocytic NOX2 electron transfer occurs from NADPH to FAD to the heme of cytb to oxygen leading to superoxide formation..
	gnl\|CDD\|145123	177	298	122	Gaps:2	100.00	122	32.79	21.31	7e-22	pfam01794 Ferric_reduct Ferric reductase like transmembrane component. This family includes a common region in the transmembrane proteins mammalian cytochrome B-245 heavy chain (gp91-phox) ferric reductase transmembrane component in yeast and respiratory burst oxidase from mouse-ear cress. This may be a family of flavocytochromes capable of moving electrons across the plasma membrane. The Frp1 protein from S. pombe is a ferric reductase component and is required for cell surface ferric reductase activity mutants in frp1 are deficient in ferric iron uptake. Cytochrome B-245 heavy chain is a FAD-dependent dehydrogenase it is also has electron transferase activity which reduces molecular oxygen to superoxide anion a precursor in the production of microbicidal oxidants. Mutations in the sequence of cytochrome B-245 heavy chain (gp91-phox) lead to the X-linked chronic granulomatous disease. The bacteriocidal ability of phagocytic cells is reduced and is characterized by the absence of a functional plasma membrane associated NADPH oxidase. The chronic granulomatous disease gene codes for the beta chain of cytochrome B-245 and cytochrome B-245 is missing from patients with the disease.
	gnl\|CDD\|149229	463	595	133	Gaps:32	99.32	148	25.17	18.37	2e-11	pfam08030 NAD_binding_6 Ferric reductase NAD binding domain.
	gnl\|CDD\|33854	142	611	470	Gaps:77	97.49	438	22.01	16.63	3e-10	COG4097 COG4097 Predicted ferric reductase [Inorganic ion transport and metabolism].
	gnl\|CDD\|166272	197	502	306	Gaps:63	38.83	703	29.67	15.02	3e-09	PLN02631 PLN02631 ferric-chelate reductase.
	gnl\|CDD\|149226	344	454	111	Gaps:26	90.29	103	30.11	21.51	1e-08	pfam08022 FAD_binding_8 FAD-binding domain.
rpsblast_kog	gnl\|CDD\|35262	107	611	505	Gaps:85	80.80	646	20.31	14.56	3e-38	KOG0039 KOG0039 KOG0039 Ferric reductase NADH/NADPH oxidase and related proteins [Inorganic ion transport and metabolism Secondary metabolites biosynthesis transport and catabolism].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	YCOA_553241	18968	19635	668	100.00	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCMA_557579	19063	19672	610	100.00	100.00	n/a	Botrytis cinerea HortResearch libraries
	PD0ACA4YL11FM1	18994	19735	742	99.87	100.00	n/a	BT4 mycelium - pH stress library
	YCMA_567957	17523	18027	399	99.50	100.00	n/a	Botrytis cinerea HortResearch libraries
	B3BC_116_232_D10	19611	20211	598	99.50	100.00	n/a	B0510 Germinated spores on hydrophobic surfaces library
sim4_Bot_allest4_clctg	CL_bt4_SuperContig_14_1_bt4ctg_0323_004.Contig1_v5	18994	20211	1215	99.75	100.00	n/a	all_lib_clone_nr:2
sim4_Ss_allest	G787P549FE7.T0	19388	20080	837	85.26	75.39	n/a	Ss ESTs grown as developing apothecia
sim4_Ss_allest	G787P527FD7.T0	8668	22925	878	86.22	94.19	n/a	Ss ESTs grown as developing apothecia
blastx_uniprot_sprot	Q08905	18254	19774	711	26.23	71.31	n/a	Ferric reductase transmembrane component 3 precursor - Saccharomyces cerevisiae (Baker's yeast)
	P53746	18278	19468	719	26.61	51.74	n/a	Ferric reductase transmembrane component 4 precursor - Saccharomyces cerevisiae (Baker's yeast)
	P36033	18269	19483	711	26.05	53.45	n/a	Ferric reductase transmembrane component 2 precursor - Saccharomyces cerevisiae (Baker's yeast)
	Q12473	17915	19774	712	23.80	84.97	n/a	Ferric reductase transmembrane component 6 precursor - Saccharomyces cerevisiae (Baker's yeast)
blastx_uniprot_trembl	Q5U9M3	17936	19774	613	99.67	100.00	n/a	FreB - Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea)
	Q7SFM3	18194	19774	549	57.56	100.00	n/a	Putative uncharacterized protein - Neurospora crassa
	Q2H477	18005	19774	631	55.83	95.09	n/a	Putative uncharacterized protein - Chaetomium globosum (Soil fungus)
	Q4WS16	18047	19774	620	46.99	96.45	n/a	Metalloreductase putative - Aspergillus fumigatus (Sartorya fumigata)
	A2QAQ6	18047	19774	619	47.57	96.45	n/a	Similarity to repressed by TUP1 protein 2 RBT2 - Candida albicans - Aspergillus niger
	A1CP28	18047	19774	621	46.74	96.46	n/a	Metalloreductase putative - Aspergillus clavatus
	A1D1Y2	18047	19774	620	45.82	96.45	n/a	Metalloreductase putative - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q6MY54	18047	19774	607	47.52	96.38	n/a	Metalloreductase putative - Aspergillus fumigatus (Sartorya fumigata)
	Q5U9M3	17936	19774	613	99.67	100.00	n/a	FreB - Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea)
blastx_FNG30_prot	SS1G_14206.1	17936	19774	615	93.17	100.00	n/a	sclerotinia_sclerotiorum hypothetical protein similar to FreB translation 616 aa
	jgi:Triree:30810	18194	19774	533	61.35	100.00	n/a	Trichoderma reesei
	NCU02009.2	18194	19774	549	57.56	100.00	n/a	Neurospora crassa hypothetical protein translation 550 aa
	CHG06538.1	18005	19774	631	55.83	95.09	n/a	Chaetomium globosum hypothetical protein translation 632 aa
	FG08525.1	17945	19732	1070	55.30	56.45	n/a	fusarium_graminearum hypothetical protein 1071 aa
	gi:70996098:ref:XP_752804.1:	18047	19774	620	46.99	96.45	n/a	Aspergillus fumigatus Af293 metalloreductase
	AO090003000630	18080	19774	592	48.13	94.76	n/a	Aspergillus oryzae ferric reductase NADH/NADPH oxidase and related proteins
	AO090011000376	18212	19774	557	49.82	98.74	n/a	Aspergillus oryzae predicted protein
	AN0773	18020	19774	627	45.68	97.77	n/a	Aspergillus nidulans AN0773.3 conserved hypothetical protein translation 628 aa
	SS1G_14206.1	17936	19774	615	93.17	100.00	n/a	sclerotinia_sclerotiorum hypothetical protein similar to FreB translation 616 aa
	SS1G_06988.1	17930	19774	614	43.19	99.19	n/a	sclerotinia_sclerotiorum hypothetical protein translation 615 aa
sim4_B0510_genes	BC1G_07704.1	17936	19777	1497	99.80	100.00	n/a	n/a
sim4_SS_genes	SS1G_14206.1	17936	19777	1848	86.96	100.00	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.48	16788	20192	276297	99.77	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.30	17978	19778	231457	86.45	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.30	17978	19778	231457	86.45	n/a	n/a	n/a
blat_oligo	botrytisP00055648	18295	18354	60	n/a	n/a	n/a	array_gene:BofuT4_P024960_1 . max_over_threshold:37.75 . min_over_threshold:0.84 . percent_hyb_over_threshold:96.42 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00055649	18466	18525	60	n/a	n/a	n/a	array_gene:BofuT4_P024960_1 . max_over_threshold:58.8 . min_over_threshold:1.85 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00055650	18602	18661	60	n/a	n/a	n/a	array_gene:BofuT4_P024960_1 . max_over_threshold:68.84 . min_over_threshold:2.22 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00055652	19009	19068	60	n/a	n/a	n/a	array_gene:BofuT4_P024960_1 . max_over_threshold:81.87 . min_over_threshold:10.2 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00055653	19278	19337	60	n/a	n/a	n/a	array_gene:BofuT4_P024960_1 . max_over_threshold:41.87 . min_over_threshold:4.63 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00055654	19319	19378	60	n/a	n/a	n/a	array_gene:BofuT4_P024960_1 . max_over_threshold:33.28 . min_over_threshold:3.04 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00055655	19474	19533	60	n/a	n/a	n/a	array_gene:BofuT4_P024960_1 . max_over_threshold:24.53 . min_over_threshold:1.87 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00055656	19481	19540	60	n/a	n/a	n/a	array_gene:BofuT4_P024960_1 . max_over_threshold:22.32 . min_over_threshold:1.87 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00055657	19568	19627	60	n/a	n/a	n/a	array_gene:BofuT4_P024960_1 . max_over_threshold:24.89 . min_over_threshold:3.18 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog