GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P024110.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_12393	1	570	570	n/a	100.00	570	89.47	0.00	0.0	hypothetical protein
	ang:An04g06250	15	569	555	Gaps:4	99.64	559	59.43	12.93	0.0	hypothetical protein
	nfi:NFIA_061830	11	568	558	Gaps:3	98.94	565	57.25	14.67	1e-170	MFS drug efflux transporter putative
	afm:AFUA_3G15250	16	568	553	Gaps:3	97.88	566	57.76	13.90	1e-168	MFS drug efflux transporter
	ani:AN9291.2	14	570	557	Gaps:4	38.98	1429	51.35	15.80	1e-155	hypothetical protein
	bfu:BC1G_12738	21	567	547	Gaps:11	97.31	557	49.08	16.61	1e-140	hypothetical protein
	tml:GSTUM_00005001001	16	569	554	Gaps:3	97.52	565	48.28	13.43	1e-137	hypothetical protein
	bfu:BC1G_00835	15	564	550	Gaps:7	98.05	564	44.85	17.00	1e-129	hypothetical protein
	act:ACLA_044420	26	570	545	Gaps:5	97.51	562	42.34	18.80	1e-126	MFS drug efflux transporter putative
	pan:PODANSg8629	24	562	539	Gaps:3	95.20	563	42.35	17.35	1e-117	hypothetical protein
blastp_uniprot_sprot	sp\|Q00357\|TOXA_COCCA	66	439	374	Gaps:17	68.07	548	31.64	19.03	4e-35	Putative HC-toxin efflux carrier TOXA OS Cochliobolus carbonum GN TOXA PE 3 SV 1
	sp\|O42922\|YBIH_SCHPO	18	440	423	Gaps:13	69.45	599	28.37	14.90	2e-32	Uncharacterized MFS-type transporter C16A3.17c OS Schizosaccharomyces pombe GN SPBC16A3.17c PE 2 SV 1
	sp\|Q10072\|YAN6_SCHPO	7	439	433	Gaps:34	72.16	589	27.53	17.65	9e-29	Uncharacterized transporter C3H1.06c OS Schizosaccharomyces pombe GN SPAC3H1.06c PE 2 SV 1
	sp\|P36172\|VBA5_YEAST	60	440	381	Gaps:42	70.62	582	28.47	14.60	3e-27	Vacuolar basic amino acid transporter 5 OS Saccharomyces cerevisiae GN VBA5 PE 3 SV 1
	sp\|Q9HE13\|YI32_SCHPO	35	303	269	Gaps:8	44.31	589	32.18	16.86	2e-26	Uncharacterized MFS-type transporter C1399.02 OS Schizosaccharomyces pombe GN SPAC1399.02 PE 2 SV 1
	sp\|O32182\|YUSP_BACSU	62	335	274	Gaps:13	48.24	541	32.95	16.86	7e-25	Uncharacterized MFS-type transporter yusP OS Bacillus subtilis GN yusP PE 3 SV 1
	sp\|P33335\|SGE1_YEAST	80	305	226	Gaps:30	46.04	543	30.80	17.20	2e-23	Protein SGE1 OS Saccharomyces cerevisiae GN SGE1 PE 1 SV 1
	sp\|O07753\|Y1877_MYCTU	62	561	500	Gaps:36	70.16	687	24.69	17.43	2e-19	Uncharacterized MFS-type transporter Rv1877/MT1926 OS Mycobacterium tuberculosis GN Rv1877 PE 3 SV 1
	sp\|P50080\|AZR1_YEAST	80	229	150	Gaps:5	24.31	613	35.57	19.46	3e-16	Azole resistance protein 1 OS Saccharomyces cerevisiae GN AZR1 PE 1 SV 1
	sp\|O59726\|YHX6_SCHPO	48	396	349	Gaps:10	59.45	577	27.41	18.08	3e-16	Uncharacterized MFS-type transporter C3E7.06c OS Schizosaccharomyces pombe GN SPBC3E7.06c PE 2 SV 1
blastp_pdb	no results
rpsblast_cdd	gnl\|CDD\|129794	74	555	482	Gaps:23	97.11	485	22.93	19.32	8e-29	TIGR00711 efflux_EmrB drug resistance transporter EmrB/QacA subfamily. This subfamily of drug efflux proteins a part of the major faciliator family is predicted to have 14 potential membrane-spanning regions. Members with known activities include EmrB (multiple drug resistance efflux pump) in E. coli FarB (antibacterial fatty acid resistance) in Neisseria gonorrhoeae TcmA (tetracenomycin C resistance) in Streptomyces glaucescens etc. In most cases the efflux pump is described as having a second component encoded in the same operon such as EmrA of E. coli.
	gnl\|CDD\|119392	67	415	349	Gaps:37	99.43	352	20.86	15.14	2e-16	cd06174 MFS The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters symporters and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions sugar phosphates drugs neurotransmitters nucleosides amino acids and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate while symporters and antiporters transport two substrates in the same or in opposite directions respectively across membranes. MFS proteins are typically 400 to 600 amino acids in length and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members GlpT (glycerol-3-phosphate transporter) LacY (lactose permease) and EmrD (multidrug transporter) MFS proteins are thought to function through a single substrate binding site alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4 which is impaired in type II diabetes and glucose-6-phosphate transporter (G6PT) which causes glycogen storage disease when mutated..
	gnl\|CDD\|148990	65	359	295	Gaps:13	86.96	345	18.00	15.67	2e-16	pfam07690 MFS_1 Major Facilitator Superfamily.
	gnl\|CDD\|170483	62	377	316	Gaps:32	63.69	471	25.67	20.67	6e-10	PRK10504 PRK10504 multidrug efflux system protein MdtE Provisional.
	gnl\|CDD\|162085	108	241	134	Gaps:5	97.16	141	28.47	15.33	3e-09	TIGR00880 2_A_01_02 Multidrug resistance protein.
	gnl\|CDD\|143864	97	241	145	Gaps:5	32.96	449	23.65	12.84	3e-08	pfam00083 Sugar_tr Sugar (and other) transporter.
	gnl\|CDD\|32643	47	277	231	Gaps:12	58.12	394	20.52	16.16	5e-07	COG2814 AraJ Arabinose efflux permease [Carbohydrate transport and metabolism].
	gnl\|CDD\|162097	91	220	130	Gaps:16	25.74	505	25.38	18.46	9e-07	TIGR00898 2A0119 cation transport protein.
rpsblast_kog	gnl\|CDD\|35475	63	454	392	Gaps:28	70.96	513	16.21	15.38	4e-13	KOG0254 KOG0254 KOG0254 Predicted transporter (major facilitator superfamily) [General function prediction only].
	gnl\|CDD\|35476	96	446	351	Gaps:21	64.88	521	20.71	15.09	4e-11	KOG0255 KOG0255 KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily) [General function prediction only].
	gnl\|CDD\|37743	102	239	138	Gaps:2	30.04	466	23.57	17.14	3e-08	KOG2532 KOG2532 KOG2532 Permease of the major facilitator superfamily [Carbohydrate transport and metabolism].
	gnl\|CDD\|37744	101	242	142	Gaps:5	29.70	495	27.21	10.20	4e-08	KOG2533 KOG2533 KOG2533 Permease of the major facilitator superfamily [Carbohydrate transport and metabolism].
	gnl\|CDD\|37715	60	483	424	Gaps:20	79.76	509	18.47	15.27	3e-07	KOG2504 KOG2504 KOG2504 Monocarboxylate transporter [Carbohydrate transport and metabolism].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	PD0AGA18YL13CM1	90061	90758	698	99.57	100.00	n/a	BT4 mycelium - different stresses library
sim4_Bot_allest4_clctg	PD0AGA18YL13CM1	90061	90758	698	99.57	100.00	n/a	BT4 mycelium - different stresses library
sim4_Ss_allest	no results
blastx_uniprot_sprot	Q00357	89165	90692	548	30.42	87.59	n/a	Putative HC-toxin efflux carrier TOXA - Cochliobolus carbonum (Bipolaris zeicola)
	Q10072	88988	90731	589	25.88	91.85	n/a	Uncharacterized transporter C3H1.06c - Schizosaccharomyces pombe (Fission yeast)
	Q09752	89153	90731	531	25.50	93.79	n/a	Multidrug resistance protein fnx1 - Schizosaccharomyces pombe (Fission yeast)
	P38358	89273	90704	474	22.44	96.84	n/a	Vacuolar basic amino acid transporter 2 - Saccharomyces cerevisiae (Baker's yeast)
blastx_uniprot_trembl	A2QJ92	89045	90746	559	66.54	97.32	n/a	Contig An04c0180 complete genome - Aspergillus niger
	Q4WZ42	89015	90749	566	64.68	98.06	n/a	MFS drug efflux transporter putative - Aspergillus fumigatus (Sartorya fumigata)
	A1D5M8	89006	90743	565	63.91	98.58	n/a	MFS drug efflux transporter putative - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q5AQY9	89039	90749	1429	58.14	38.28	n/a	Putative uncharacterized protein - Emericella nidulans (Aspergillus nidulans)
	Q0CJC4	89045	90743	577	49.02	96.88	n/a	Predicted protein - Aspergillus terreus (strain NIH 2624)
	A1DEF3	89069	90734	556	48.48	94.96	n/a	MFS transporter putative - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
blastx_FNG30_prot	gi:70998755:ref:XP_754099.1:	89015	90749	566	64.68	98.06	n/a	Aspergillus fumigatus Af293 MFS toxin efflux pump
	AN9291	89039	90749	1429	58.14	38.28	n/a	Aspergillus nidulans AN9291.3 conserved hypothetical protein translation 1430 aa
	SS1G_03247.1	89003	90746	579	46.71	97.24	n/a	sclerotinia_sclerotiorum hypothetical protein translation 580 aa
	gi:70997746:ref:XP_753608.1:	89039	90734	556	46.68	97.48	n/a	Aspergillus fumigatus Af293 MFS transporter
	AN9219	89108	90737	553	46.55	94.39	n/a	Aspergillus nidulans AN9219.3 conserved hypothetical protein translation 554 aa
	CHG10645.1	89129	90725	551	44.31	92.56	n/a	Chaetomium globosum hypothetical protein translation 552 aa
	AO090005000510	89120	90635	552	44.58	86.96	n/a	Aspergillus oryzae permeases of the major facilitator superfamily
	CIMG_02396.2	89156	90722	731	40.64	67.99	n/a	Coccidioides immitis hypothetical protein translation 732 aa
	SNU11072.1	89117	90722	565	38.99	90.80	n/a	Stagonospora nodorum hypothetical protein translation
	CHG10749.1	89111	90725	559	48.73	91.77	n/a	Chaetomium globosum hypothetical protein translation 560 aa
sim4_B0510_genes	BC1G_12393.1	88970	90752	1713	100.00	100.00	n/a	n/a
sim4_SS_genes	no results
blastn_b0510_supercontig	B0510_supercontig_1.104	83710	98615	157244	99.99	n/a	n/a	n/a
blastn_SS_supercontig	no results
blat_oligo	botrytisP00055018	89246	89305	60	n/a	n/a	n/a	array_gene:BofuT4_P024110_1 . max_over_threshold:21.12 . min_over_threshold:0.27 . percent_hyb_over_threshold:25 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00055019	89385	89444	60	n/a	n/a	n/a	array_gene:BofuT4_P024110_1 . max_over_threshold:32.53 . min_over_threshold:0.58 . percent_hyb_over_threshold:60.71 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:1
	botrytisP00055020	89478	89537	60	n/a	n/a	n/a	array_gene:BofuT4_P024110_1 . max_over_threshold:50.49 . min_over_threshold:0.45 . percent_hyb_over_threshold:71.42 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:1
	botrytisP00055022	89889	89948	60	n/a	n/a	n/a	array_gene:BofuT4_P024110_1 . max_over_threshold:88.05 . min_over_threshold:0.51 . percent_hyb_over_threshold:92.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:1
	botrytisP00055023	90105	90164	60	n/a	n/a	n/a	array_gene:BofuT4_P024110_1 . max_over_threshold:20.19 . min_over_threshold:0.36 . percent_hyb_over_threshold:42.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00055024	90176	90235	60	n/a	n/a	n/a	array_gene:BofuT4_P024110_1 . max_over_threshold:22.05 . min_over_threshold:0.48 . percent_hyb_over_threshold:46.42 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00055025	90205	90264	60	n/a	n/a	n/a	array_gene:BofuT4_P024110_1 . max_over_threshold:17.39 . min_over_threshold:0.31 . percent_hyb_over_threshold:17.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00055026	90405	90464	60	n/a	n/a	n/a	array_gene:BofuT4_P024110_1 . max_over_threshold:2.9 . min_over_threshold:0.53 . percent_hyb_over_threshold:7.14 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00055027	90621	90680	60	n/a	n/a	n/a	array_gene:BofuT4_P024110_1 . max_over_threshold:45.62 . min_over_threshold:0.6 . percent_hyb_over_threshold:82.14 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog