GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P019130.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_00816	1	519	519	n/a	99.05	524	96.53	0.39	0.0	hypothetical protein
	aor:AO090138000196	9	587	579	Gaps:12	98.80	582	58.26	13.91	0.0	2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1 7-dioic acid hydratase (catechol pathway)
	afv:AFLA_124570	9	587	579	Gaps:12	98.80	582	58.26	14.09	0.0	fumarylacetoacetate hydrolase putative
	ani:AN2777.2	13	591	579	Gaps:11	99.15	587	58.76	13.57	0.0	hypothetical protein
	pno:SNOG_01713	10	564	555	Gaps:19	73.87	731	55.37	13.89	1e-161	hypothetical protein
	afv:AFLA_059120	9	591	583	Gaps:11	55.30	1067	51.69	15.42	1e-160	fumarylacetoacetate hydrolase putative
	tml:GSTUM_00009119001	11	592	582	Gaps:33	98.64	589	51.98	13.77	1e-150	hypothetical protein
	aor:AO090701000450	9	377	369	Gaps:9	43.14	867	57.22	16.04	1e-117	2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1 7-dioic acid hydratase (catechol pathway)
	rha:RHA1_ro04298	40	591	552	Gaps:38	95.12	553	44.87	15.97	1e-100	hydrolase
	cnb:CNBC0420	1	299	299	Gaps:15	86.90	336	51.37	16.44	6e-70	hypothetical protein
blastp_uniprot_sprot	sp\|O06724\|YISK_BACSU	70	298	229	Gaps:11	75.08	301	50.44	21.68	1e-60	Uncharacterized protein yisK OS Bacillus subtilis GN yisK PE 2 SV 1
	sp\|Q8CPT8\|Y665_STAES	69	297	229	Gaps:13	73.75	301	45.05	17.57	1e-44	Uncharacterized protein SE_0665 OS Staphylococcus epidermidis (strain ATCC 12228) GN SE_0665 PE 3 SV 1
	sp\|Q5HQJ3\|Y556_STAEQ	69	297	229	Gaps:13	73.75	301	45.05	17.57	1e-44	Uncharacterized protein SERP0556 OS Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN SERP0556 PE 3 SV 1
	sp\|Q4L4Y4\|Y1982_STAHJ	69	299	231	Gaps:21	74.42	301	44.20	19.20	3e-44	Uncharacterized protein SH1982 OS Staphylococcus haemolyticus (strain JCSC1435) GN SH1982 PE 3 SV 1
	sp\|Q5HHB6\|Y973_STAAC	69	299	231	Gaps:21	74.67	300	44.64	18.30	3e-44	Uncharacterized protein SACOL0973 OS Staphylococcus aureus (strain COL) GN SACOL0973 PE 3 SV 1
	sp\|Q99VC2\|Y968_STAAM	69	299	231	Gaps:21	74.67	300	44.64	18.30	3e-44	Uncharacterized protein SAV0968 OS Staphylococcus aureus (strain Mu50 / ATCC 700699) GN SAV0968 PE 1 SV 1
	sp\|Q2FZT4\|Y906_STAA8	69	299	231	Gaps:21	74.67	300	44.64	18.30	3e-44	Uncharacterized protein SAOUHSC_00906 OS Staphylococcus aureus (strain NCTC 8325) GN SAOUHSC_00906 PE 3 SV 1
	sp\|Q2FIA7\|Y871_STAA3	69	299	231	Gaps:21	74.67	300	44.64	18.30	3e-44	Uncharacterized protein SAUSA300_0871 OS Staphylococcus aureus (strain USA300) GN SAUSA300_0871 PE 3 SV 1
	sp\|Q7A1B6\|Y850_STAAW	69	299	231	Gaps:21	74.67	300	44.64	18.30	3e-44	Uncharacterized protein MW0850 OS Staphylococcus aureus (strain MW2) GN MW0850 PE 3 SV 1
	sp\|Q6GAV8\|Y838_STAAS	69	299	231	Gaps:21	74.67	300	44.64	18.30	3e-44	Uncharacterized protein SAS0838 OS Staphylococcus aureus (strain MSSA476) GN SAS0838 PE 3 SV 1
blastp_pdb	2dfu_D	55	302	248	Gaps:25	92.05	264	38.27	17.28	1e-34	mol:protein length:264 probable 2-hydroxyhepta-2 4-diene-1 7-dioate
	2dfu_C	55	302	248	Gaps:25	92.05	264	38.27	17.28	1e-34	mol:protein length:264 probable 2-hydroxyhepta-2 4-diene-1 7-dioate
	2dfu_B	55	302	248	Gaps:25	92.05	264	38.27	17.28	1e-34	mol:protein length:264 probable 2-hydroxyhepta-2 4-diene-1 7-dioate
	2dfu_A	55	302	248	Gaps:25	92.05	264	38.27	17.28	1e-34	mol:protein length:264 probable 2-hydroxyhepta-2 4-diene-1 7-dioate
	1wzo_D	60	299	240	Gaps:21	91.46	246	36.44	19.56	2e-28	mol:protein length:246 HpcE
	1wzo_C	60	299	240	Gaps:21	91.46	246	36.44	19.56	2e-28	mol:protein length:246 HpcE
	1wzo_B	60	299	240	Gaps:21	91.46	246	36.44	19.56	2e-28	mol:protein length:246 HpcE
	1wzo_A	60	299	240	Gaps:21	91.46	246	36.44	19.56	2e-28	mol:protein length:246 HpcE
	1i7o_D	86	297	212	Gaps:29	80.89	429	36.60	17.29	3e-28	mol:protein length:429 4-HYDROXYPHENYLACETATE DEGRADATION BIFUNCTION
	1i7o_C	86	297	212	Gaps:29	80.89	429	36.60	17.29	3e-28	mol:protein length:429 4-HYDROXYPHENYLACETATE DEGRADATION BIFUNCTION
rpsblast_cdd	gnl\|CDD\|30528	41	297	257	Gaps:20	92.86	266	42.11	15.79	2e-57	COG0179 MhpD 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1 7-dioic acid hydratase (catechol pathway) [Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|144959	86	297	212	Gaps:15	99.04	209	40.10	16.91	7e-52	pfam01557 FAA_hydrolase Fumarylacetoacetate (FAA) hydrolase family. This family consists of fumarylacetoacetate (FAA) hydrolase or fumarylacetoacetate hydrolase (FAH) and it also includes HHDD isomerase/OPET decarboxylase from E. coli strain W. FAA is the last enzyme in the tyrosine catabolic pathway it hydrolyses fumarylacetoacetate into fumarate and acetoacetate which then join the citric acid cycle. Mutations in FAA cause type I tyrosinemia in humans this is an inherited disorder mainly affecting the liver leading to liver cirrhosis hepatocellular carcinoma renal tubular damages and neurologic crises amongst other symptoms. The enzymatic defect causes the toxic accumulation of phenylalanine/tyrosine catabolites. The E. coli W enzyme HHDD isomerase/OPET decarboxylase contains two copies of this domain and functions in fourth and fifth steps of the homoprotocatechuate pathway here it decarboxylates OPET to HHDD and isomerizes this to OHED. The final products of this pathway are pyruvic acid and succinic semialdehyde. This family also includes various hydratases and 4-oxalocrotonate decarboxylases which are involved in the bacterial meta-cleavage pathways for degradation of aromatic compounds. 2-hydroxypentadienoic acid hydratase encoded by mhpD in E. coli is involved in the phenylpropionic acid pathway of E. coli and catalyses the conversion of 2-hydroxy pentadienoate to 4-hydroxy-2-keto-pentanoate and uses a Mn2+ co-factor. OHED hydratase encoded by hpcG in E. coli is involved in the homoprotocatechuic acid (HPC) catabolism. XylI in P. putida is a 4-Oxalocrotonate decarboxylase.
	gnl\|CDD\|131356	51	297	247	Gaps:22	95.10	245	36.91	17.60	1e-39	TIGR02303 HpaG-C-term 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase C-terminal subunit. This model represents one of two subunits/domains of the bifunctional isomerase/decarboxylase involved in 4-hydroxyphenylacetate degradation. In E. coli and some other species this enzyme is encoded by a single polypeptide containing both this domain and the closely related N-terminal domain (TIGR02305). In other species such as Pasteurella multocida these domains are found as two separate proteins (usually as tandem genes). Together these domains carry out the decarboxylation of 5-oxopent-3-ene-1 2 5-tricarboxylic acid (OPET) to 2-hydroxy-2 4-diene-1 7-dioate (HHDD) and the subsequent isomerization to 2-oxohept-3-ene-1 7-dioate (OHED).
	gnl\|CDD\|131480	338	573	236	Gaps:15	92.03	251	32.47	14.72	3e-32	TIGR02427 protocat_pcaD 3-oxoadipate enol-lactonase. Members of this family are 3-oxoadipate enol-lactonase. Note that the substrate is known as 3-oxoadipate enol-lactone 2-oxo-2 3-dihydrofuran-5-acetate 4 5-Dihydro-5-oxofuran-2-acetate and 5-oxo-4 5-dihydrofuran-2-acetate. The enzyme the catalyzes the fourth step in the protocatechuate degradation to beta-ketoadipate and then to succinyl-CoA and acetyl-CoA. 4-hydroxybenzoate 3-hydroxybenzoate and vanillate all can be converted in one step to protocatechuate. This enzyme also acts in catechol degradation. In genomes that catabolize both catechol and protocatechuate two forms of this enzyme may be found. All members of the seed alignment for this model were chosen from within protocatechuate degradation operons of at least three genes of the pathway from genomes with the complete pathway through beta-ketoadipate.
	gnl\|CDD\|30941	332	592	261	Gaps:22	97.52	282	28.73	14.91	2e-28	COG0596 MhpC Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) [General function prediction only].
	gnl\|CDD\|171711	84	288	205	Gaps:20	37.72	501	38.62	13.23	7e-27	PRK12764 PRK12764 hypothetical protein Provisional.
	gnl\|CDD\|131358	114	297	184	Gaps:22	85.85	205	33.52	19.32	3e-25	TIGR02305 HpaG-N-term 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase N-terminal subunit. This model represents one of two subunits/domains of the bifunctional isomerase/decarboxylase involved in 4-hydroxyphenylacetate degradation. In E. coli and some other species this enzyme is encoded by a single polypeptide containing both this domain and the closely related C-terminal domain (TIGR02303). In other species such as Pasteurella multocida these domains are found as two separate proteins (usually as tandem genes). Together these domains carry out the decarboxylation of 5-oxopent-3-ene-1 2 5-tricarboxylic acid (OPET) to 2-hydroxy-2 4-diene-1 7-dioate (HHDD) and the subsequent isomerization to 2-oxohept-3-ene-1 7-dioate (OHED).
	gnl\|CDD\|144228	382	587	206	Gaps:18	96.89	225	23.39	14.68	3e-19	pfam00561 Abhydrolase_1 alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes.
	gnl\|CDD\|170634	86	289	204	Gaps:25	88.13	219	32.64	19.17	7e-18	PRK10691 PRK10691 hypothetical protein Provisional.
	gnl\|CDD\|32448	330	580	251	Gaps:28	88.26	298	21.29	13.31	4e-14	COG2267 PldB Lysophospholipase [Lipid metabolism].
rpsblast_kog	gnl\|CDD\|36748	73	297	225	Gaps:25	98.62	217	40.19	16.82	6e-39	KOG1535 KOG1535 KOG1535 Predicted fumarylacetoacetate hydralase [General function prediction only].
	gnl\|CDD\|36667	335	592	258	Gaps:24	85.28	326	23.02	15.83	1e-17	KOG1454 KOG1454 KOG1454 Predicted hydrolase/acyltransferase (alpha/beta hydrolase superfamily) [General function prediction only].
	gnl\|CDD\|39610	332	450	119	Gaps:7	33.42	365	27.05	14.75	3e-12	KOG4409 KOG4409 KOG4409 Predicted hydrolase/acyltransferase (alpha/beta hydrolase superfamily) [General function prediction only].
	gnl\|CDD\|39380	332	592	261	Gaps:36	90.37	322	21.31	14.78	2e-10	KOG4178 KOG4178 KOG4178 Soluble epoxide hydrolase [Lipid transport and metabolism].
	gnl\|CDD\|37593	338	456	119	Gaps:7	38.73	315	27.87	15.57	5e-08	KOG2382 KOG2382 KOG2382 Predicted alpha/beta hydrolase [General function prediction only].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	YCIA_547240	857365	857961	597	99.83	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCHA_534928	857249	857952	648	99.85	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCHA_543455	857202	857942	685	99.85	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCHA_538256	857179	857942	708	99.72	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCHA_535310	857365	857942	578	99.83	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCHA_539156	857365	857942	578	99.65	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCGA_543964	857446	857964	519	99.61	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCGA_541841	857477	857865	389	99.74	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCHA_532475	857600	857969	370	99.73	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCGA_541853	857546	857865	320	99.38	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCIA_545362	857632	857863	233	98.28	100.00	n/a	Botrytis cinerea HortResearch libraries
	BcA1_3474_D14	856068	856779	713	99.30	100.00	n/a	BC A1 mycelium
	YCOA_554367	857365	858021	657	99.85	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCOA_554004	857253	858021	713	99.72	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCMA_561822	857368	858021	654	99.85	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCOA_551943	857386	858021	636	99.69	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCOA_549236	857388	858021	634	99.68	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCOA_570519	857397	858021	625	99.84	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCOA_552278	857397	858021	625	99.68	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCOA_552290	857538	858021	484	99.79	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCOA_555951	857562	858021	460	99.78	100.00	n/a	Botrytis cinerea HortResearch libraries
	YCOA_568783	857586	858021	436	99.77	100.00	n/a	Botrytis cinerea HortResearch libraries
sim4_Bot_allest4_clctg	BcA1_3474_D14	856068	856779	713	99.30	100.00	n/a	BC A1 mycelium 4 days in rich media library
sim4_Ss_allest	no results
blastx_uniprot_sprot	Q8CPT8	857022	857770	301	44.64	74.42	n/a	Uncharacterized protein SE_0665 - Staphylococcus epidermidis (strain ATCC 12228)
	Q5HQJ3	857022	857770	301	44.64	74.42	n/a	Uncharacterized protein SERP0556 - Staphylococcus epidermidis (strain ATCC 35984 / RP62A)
	Q5HHB6	857022	857770	300	44.20	74.67	n/a	Uncharacterized protein SACOL0973 - Staphylococcus aureus (strain COL)
	Q99VC2	857022	857770	300	44.20	74.67	n/a	Uncharacterized protein SAV0968 - Staphylococcus aureus (strain Mu50 / ATCC 700699)
	Q2FZT4	857022	857770	300	44.20	74.67	n/a	Uncharacterized protein SAOUHSC_00906 - Staphylococcus aureus (strain NCTC 8325)
	Q2FIA7	857022	857770	300	44.20	74.67	n/a	Uncharacterized protein SAUSA300_0871 - Staphylococcus aureus (strain USA300)
	Q7A1B6	857022	857770	300	44.20	74.67	n/a	Uncharacterized protein MW0850 - Staphylococcus aureus (strain MW2)
	Q6GAV8	857022	857770	300	44.20	74.67	n/a	Uncharacterized protein SAS0838 - Staphylococcus aureus (strain MSSA476)
	Q2YWW3	857022	857770	300	44.20	74.67	n/a	Uncharacterized protein SAB0837 - Staphylococcus aureus (strain bovine RF122)
	Q4L4Y4	857022	857770	301	43.30	74.42	n/a	Uncharacterized protein SH1982 - Staphylococcus haemolyticus (strain JCSC1435)
blastx_uniprot_trembl	Q5B9K3	856146	857938	587	60.48	99.15	n/a	Putative uncharacterized protein - Emericella nidulans (Aspergillus nidulans)
	Q2U1K8	856158	857950	582	60.00	98.80	n/a	RIB40 genomic DNA SC138 - Aspergillus oryzae
	Q0V2Q1	856236	857956	731	56.85	73.87	n/a	Putative uncharacterized protein - Phaeosphaeria nodorum (Septoria nodorum)
	Q2U8F5	856791	857947	867	n/a	n/a	n/a	RIB40 genomic DNA SC111 - Aspergillus oryzae
	Q0S8P7	856146	857857	553	46.01	95.12	n/a	Probable hydrolase - Rhodococcus sp. (strain RHA1)
	Q5KJF0	857022	857974	336	51.37	86.90	n/a	Mitochondrion protein putative - Cryptococcus neoformans (Filobasidiella neoformans)
	A9IA49	857022	857905	301	48.94	93.69	n/a	Fumarylacetoacetate hydrolase family protein - Bordetella petrii
	Q7WFY8	857025	857905	293	49.29	96.25	n/a	Putative enzyme - Bordetella bronchiseptica (Alcaligenes bronchisepticus)
	Q7W4G5	857025	857905	293	48.94	96.25	n/a	Putative enzyme - Bordetella parapertussis
blastx_FNG30_prot	AN2777	856146	857938	587	60.48	99.15	n/a	Aspergillus nidulans AN2777.3 conserved hypothetical protein translation 588 aa
	AO090138000196	856158	857950	582	60.00	98.80	n/a	Aspergillus oryzae 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-17-dioic acid hydratase catechol pathway
	SNU01712.1	856146	857956	593	56.19	98.15	n/a	Stagonospora nodorum hypothetical protein translation
	jgi:Triree:35032	856161	857956	639	53.58	91.71	n/a	Trichoderma reesei
	AO090701000450	856791	857947	867	n/a	n/a	n/a	Aspergillus oryzae 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-17-dioic acid hydratase catechol pathway
	gi:58265408:ref:XP_569860.1	857022	857974	336	51.37	86.90	n/a	Cryptococcus neoformans var. neoformans JEC21 mitochondrion protein
	CHG00013.1	857028	857635	247	43.92	76.52	n/a	Chaetomium globosum hypothetical protein translation 248 aa
	CHG01067.1	857028	857746	298	44.76	70.47	n/a	Chaetomium globosum hypothetical protein translation 299 aa
	CIMG_07272.2	857028	857749	289	41.63	76.47	n/a	Coccidioides immitis hypothetical protein translation 290 aa
	NCU07506.2	857010	857743	311	41.89	71.38	n/a	Neurospora crassa hypothetical protein translation 312 aa
sim4_B0510_genes	BC1G_00816.1	856346	857974	1575	99.43	100.00	n/a	n/a
sim4_SS_genes	no results
blastn_b0510_supercontig	B0510_supercontig_1.2	849382	868790	1005568	99.91	n/a	n/a	n/a
blastn_SS_supercontig	no results
blat_oligo	botrytisP00051068	857625	857684	60	n/a	n/a	n/a	array_gene:BofuT4_P019130_1 . max_over_threshold:12.86 . min_over_threshold:0.93 . percent_hyb_over_threshold:96.42 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00051069	857411	857470	60	n/a	n/a	n/a	array_gene:BofuT4_P019130_1 . max_over_threshold:36.56 . min_over_threshold:2.35 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00051070	857286	857401	60	n/a	n/a	n/a	array_gene:BofuT4_P019130_1 . max_over_threshold:32.27 . min_over_threshold:2.18 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00051072	857274	857389	60	n/a	n/a	n/a	array_gene:BofuT4_P019130_1 . max_over_threshold:37.21 . min_over_threshold:1.65 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00051073	857117	857176	60	n/a	n/a	n/a	array_gene:BofuT4_P019130_1 . max_over_threshold:23.33 . min_over_threshold:1.44 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00051074	856855	856914	60	n/a	n/a	n/a	array_gene:BofuT4_P019130_1 . max_over_threshold:13.52 . min_over_threshold:1.16 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00051075	856619	856678	60	n/a	n/a	n/a	array_gene:BofuT4_P019130_1 . max_over_threshold:26.42 . min_over_threshold:1.84 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00051076	856445	856504	60	n/a	n/a	n/a	array_gene:BofuT4_P019130_1 . max_over_threshold:23.93 . min_over_threshold:1.23 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00051077	856326	856385	60	n/a	n/a	n/a	array_gene:BofuT4_P019130_1 . max_over_threshold:24.78 . min_over_threshold:1.34 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog