GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P015360.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_15446	1	290	290	n/a	100.00	290	93.10	0.00	1e-154	hypothetical protein
	ssl:SS1G_00009	46	290	245	n/a	94.59	259	63.27	13.88	2e-87	hypothetical protein
	bgf:BC1003_5829	12	278	267	Gaps:18	90.88	274	40.56	18.07	1e-34	alpha/beta hydrolase fold protein
	bcj:BCAL2536A	24	278	255	Gaps:16	91.22	262	38.49	15.48	2e-29	putative hydrolase
	bcj:BCAL2578	28	279	252	Gaps:16	88.39	267	37.71	17.80	5e-29	putative hydrolase
	act:ACLA_093850	27	277	251	Gaps:33	90.85	295	36.19	16.42	4e-28	alpha/beta fold family hydrolase putative
	bxe:Bxe_B2696	10	281	272	Gaps:18	65.63	387	36.22	16.54	9e-28	putative hydrolase
	afv:AFLA_090180	22	274	253	Gaps:36	91.55	296	35.42	15.50	1e-27	alpha/beta hydrolase putative
	aor:AO090120000022	22	274	253	Gaps:36	91.55	296	35.42	15.50	2e-27	hypothetical protein
	rpf:Rpic12D_1227	10	281	272	Gaps:38	65.63	387	37.40	18.11	4e-27	alpha/beta hydrolase fold protein
blastp_uniprot_sprot	sp\|O31581\|YFHM_BACSU	15	116	102	Gaps:10	34.97	286	38.00	15.00	2e-07	AB hydrolase superfamily protein yfhM OS Bacillus subtilis GN yfhM PE 3 SV 1
blastp_pdb	no results
rpsblast_cdd	gnl\|CDD\|163407	26	277	252	Gaps:43	99.20	251	30.12	17.67	1e-17	TIGR03695 menH_SHCHC 2-succinyl-6-hydroxy-2 4-cyclohexadiene-1-carboxylate synthase. This protein catalyzes the formation of SHCHC or (1 R 6 R)-2-succinyl-6-hydroxy-2 4-cyclohexadiene-1-carboxylate by elmination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1- carboxylate (SEPHCHC). Note that SHCHC synthase activity previously was attributed to MenD which in fact is SEPHCHC synthase.
	gnl\|CDD\|30941	26	281	256	Gaps:26	92.91	282	27.48	13.36	1e-16	COG0596 MhpC Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) [General function prediction only].
	gnl\|CDD\|32448	28	267	240	Gaps:30	81.21	298	28.10	11.57	2e-14	COG2267 PldB Lysophospholipase [Lipid metabolism].
	gnl\|CDD\|144228	55	275	221	Gaps:41	98.67	225	22.97	18.47	9e-14	pfam00561 Abhydrolase_1 alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes.
	gnl\|CDD\|131480	21	279	259	Gaps:43	97.21	251	27.87	17.21	7e-11	TIGR02427 protocat_pcaD 3-oxoadipate enol-lactonase. Members of this family are 3-oxoadipate enol-lactonase. Note that the substrate is known as 3-oxoadipate enol-lactone 2-oxo-2 3-dihydrofuran-5-acetate 4 5-Dihydro-5-oxofuran-2-acetate and 5-oxo-4 5-dihydrofuran-2-acetate. The enzyme the catalyzes the fourth step in the protocatechuate degradation to beta-ketoadipate and then to succinyl-CoA and acetyl-CoA. 4-hydroxybenzoate 3-hydroxybenzoate and vanillate all can be converted in one step to protocatechuate. This enzyme also acts in catechol degradation. In genomes that catabolize both catechol and protocatechuate two forms of this enzyme may be found. All members of the seed alignment for this model were chosen from within protocatechuate degradation operons of at least three genes of the pathway from genomes with the complete pathway through beta-ketoadipate.
	gnl\|CDD\|170968	27	134	108	Gaps:17	39.26	242	34.74	18.95	2e-10	PRK11126 PRK11126 acyl-CoA thioester hydrolase YfbB Provisional.
	gnl\|CDD\|33021	26	274	249	Gaps:26	91.39	244	19.28	14.35	1e-07	COG3208 GrsT Predicted thioesterase involved in non-ribosomal peptide biosynthesis [Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|144533	27	274	248	Gaps:32	98.21	224	22.73	13.18	2e-07	pfam00975 Thioesterase Thioesterase domain. Peptide synthetases are involved in the non-ribosomal synthesis of peptide antibiotics. Next to the operons encoding these enzymes in almost all cases are genes that encode proteins that have similarity to the type II fatty acid thioesterases of vertebrates. There are also modules within the peptide synthetases that also share this similarity. With respect to antibiotic production thioesterases are required for the addition of the last amino acid to the peptide antibiotic thereby forming a cyclic antibiotic. Thioesterases (non-integrated) have molecular masses of 25-29 kDa.
	gnl\|CDD\|33128	27	267	241	Gaps:27	91.83	257	23.73	12.71	2e-07	COG3319 COG3319 Thioesterase domains of type I polyketide synthases or non-ribosomal peptide synthetases [Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|168274	1	106	106	Gaps:2	17.90	581	26.92	19.23	7e-07	PRK05855 PRK05855 short chain dehydrogenase Validated.
rpsblast_kog	gnl\|CDD\|36667	15	278	264	Gaps:29	84.36	326	24.00	17.09	6e-16	KOG1454 KOG1454 KOG1454 Predicted hydrolase/acyltransferase (alpha/beta hydrolase superfamily) [General function prediction only].
	gnl\|CDD\|39380	18	279	262	Gaps:47	87.89	322	22.97	12.72	6e-12	KOG4178 KOG4178 KOG4178 Soluble epoxide hydrolase [Lipid transport and metabolism].
	gnl\|CDD\|37775	10	121	112	Gaps:11	34.11	343	34.19	17.09	7e-10	KOG2564 KOG2564 KOG2564 Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold [General function prediction only].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	bt4_Recon_143_Malign_3:NoCat	21159	21239	1384	n/a	n/a	n/a	no match with RU
sim4_Bot_allest	no results
sim4_Bot_allest4_clctg	no results
sim4_Ss_allest	G786P522RD11.T0	21496	21982	735	87.23	50.07	n/a	Ss ESTs grown as mycelia at neutral pH
sim4_Ss_allest	G787P594FC12.T0	21937	22863	829	87.70	51.99	n/a	Ss ESTs grown as developing apothecia
blastx_uniprot_sprot	no results
blastx_uniprot_trembl	A1CFN7	21140	21892	295	40.30	90.85	n/a	Alpha/beta fold family hydrolase putative - Aspergillus clavatus
	Q2U704	21149	21937	296	35.94	94.93	n/a	Predicted protein - Aspergillus oryzae
	A0GP66	21128	21943	387	39.76	65.63	n/a	Alpha/beta hydrolase fold - Burkholderia phytofirmans PsJN
	Q13RJ2	21128	21943	387	40.55	65.63	n/a	Putative hydrolase - Burkholderia xenovorans (strain LB400)
	A7CGP9	21128	21943	387	38.58	65.63	n/a	Alpha/beta hydrolase fold - Ralstonia pickettii 12D
	Q49BE6	21128	21901	263	37.04	92.40	n/a	Putative hydrolase - Streptomyces hygroscopicus
	Q13FA0	21128	21949	387	40.47	66.41	n/a	Putative hydrolase - Burkholderia xenovorans (strain LB400)
	A2QTD6	21134	21895	298	35.42	90.94	n/a	Contig An09c0040 complete genome. - Aspergillus niger
blastx_FNG30_prot	AO090120000022	21149	21937	296	35.94	94.93	n/a	Aspergillus oryzae predicted protein
blastx_FNG30_prot	SS1G_00009.1	21077	21829	259	68.53	96.91	n/a	sclerotinia_sclerotiorum hypothetical protein translation 260 aa
sim4_B0510_genes	BC1G_15446.1	21098	21970	873	99.89	100.00	n/a	n/a
sim4_SS_genes	SS1G_00009.1	21159	21829	780	60.98	70.64	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.181	9134	22365	55464	99.90	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.1	21481	21974	2777073	82.79	n/a	n/a	n/a
	SS_supercontig_1.1	21273	21448	2777073	80.68	n/a	n/a	n/a
	SS_supercontig_1.1	21481	21974	2777073	82.79	n/a	n/a	n/a
blat_oligo	botrytisP00048192	21869	21928	60	n/a	n/a	n/a	array_gene:BofuT4_P015360_1 . max_over_threshold:32.6 . min_over_threshold:2.33 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00048193	21836	21895	60	n/a	n/a	n/a	array_gene:BofuT4_P015360_1 . max_over_threshold:24.43 . min_over_threshold:2.13 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00048194	21720	21779	60	n/a	n/a	n/a	array_gene:BofuT4_P015360_1 . max_over_threshold:29.39 . min_over_threshold:2.81 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00048196	21529	21588	60	n/a	n/a	n/a	array_gene:BofuT4_P015360_1 . max_over_threshold:25.21 . min_over_threshold:2.17 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00048197	21503	21562	60	n/a	n/a	n/a	array_gene:BofuT4_P015360_1 . max_over_threshold:8.52 . min_over_threshold:1.19 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:1
	botrytisP00048198	21393	21452	60	n/a	n/a	n/a	array_gene:BofuT4_P015360_1 . max_over_threshold:6.47 . min_over_threshold:0.61 . percent_hyb_over_threshold:75 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00048199	21382	21441	60	n/a	n/a	n/a	array_gene:BofuT4_P015360_1 . max_over_threshold:7.17 . min_over_threshold:0.8 . percent_hyb_over_threshold:82.14 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00048200	21289	21348	60	n/a	n/a	n/a	array_gene:BofuT4_P015360_1 . max_over_threshold:21.8 . min_over_threshold:2.07 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00048201	21114	21173	60	n/a	n/a	n/a	array_gene:BofuT4_P015360_1 . max_over_threshold:10.4 . min_over_threshold:1.11 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog