GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4_P007050.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_07743	293	684	392	n/a	97.03	404	80.10	0.00	1e-175	hypothetical protein
	bfu:BC1G_07742	1	218	218	n/a	99.09	220	98.62	0.46	1e-125	hypothetical protein
	ssl:SS1G_07922	11	276	266	Gaps:25	52.01	498	59.46	13.90	7e-77	hypothetical protein
	ncr:NCU05722	33	376	344	Gaps:74	61.30	522	29.06	19.06	5e-14	hypothetical protein
	bfu:BC1G_02418	9	286	278	Gaps:55	52.58	523	30.18	18.55	1e-13	hypothetical protein
	ssl:SS1G_09709	28	224	197	Gaps:40	20.85	820	31.58	22.22	4e-12	hypothetical protein
	mgr:MGG_08709	33	380	348	Gaps:75	45.94	764	28.77	18.23	1e-11	MG08709.4 hypothetical protein
	ctp:CTRG_01934	143	386	244	Gaps:39	47.28	459	30.88	20.74	7e-11	similar to protein-serine/threonine kinase K08857 NIMA (never in mitosis gene a)-related kinase [EC:2.7.11.1]
	clu:CLUG_05229	143	387	245	Gaps:50	39.10	555	33.64	17.51	1e-09	hypothetical protein K08857 NIMA (never in mitosis gene a)-related kinase [EC:2.7.11.1]
	pgu:PGUG_02304	143	372	230	Gaps:42	44.16	462	33.82	17.16	5e-09	hypothetical protein K08857 NIMA (never in mitosis gene a)-related kinase [EC:2.7.11.1]
blastp_uniprot_sprot	sp\|Q86UE8\|TLK2_HUMAN	209	368	160	Gaps:12	19.17	772	33.11	18.24	2e-09	Serine/threonine-protein kinase tousled-like 2 OS Homo sapiens GN TLK2 PE 1 SV 2
	sp\|O55047\|TLK2_MOUSE	209	368	160	Gaps:12	20.61	718	33.11	18.24	2e-09	Serine/threonine-protein kinase tousled-like 2 OS Mus musculus GN Tlk2 PE 2 SV 2
	sp\|Q08CW1\|TLK2_XENTR	145	368	224	Gaps:32	27.55	697	31.77	19.79	2e-09	Serine/threonine-protein kinase tousled-like 2 OS Xenopus tropicalis GN tlk2 PE 2 SV 1
	sp\|Q8RXT4\|NEK4_ARATH	143	369	227	Gaps:52	31.53	555	36.00	16.57	5e-09	Serine/threonine-protein kinase Nek4 OS Arabidopsis thaliana GN NEK4 PE 2 SV 1
	sp\|Q9UKI8\|TLK1_HUMAN	209	368	160	Gaps:13	19.19	766	31.97	17.69	2e-08	Serine/threonine-protein kinase tousled-like 1 OS Homo sapiens GN TLK1 PE 1 SV 2
	sp\|Q8C0V0\|TLK1_MOUSE	209	368	160	Gaps:13	19.19	766	31.97	17.69	2e-08	Serine/threonine-protein kinase tousled-like 1 OS Mus musculus GN Tlk1 PE 2 SV 2
	sp\|Q9UIK4\|DAPK2_HUMAN	137	369	233	Gaps:56	50.00	370	33.51	18.38	3e-08	Death-associated protein kinase 2 OS Homo sapiens GN DAPK2 PE 1 SV 1
	sp\|Q8VDF3\|DAPK2_MOUSE	137	369	233	Gaps:56	50.00	370	33.51	18.38	3e-08	Death-associated protein kinase 2 OS Mus musculus GN Dapk2 PE 1 SV 1
	sp\|Q90ZY6\|TLK1B_DANRE	209	368	160	Gaps:13	19.44	756	31.97	17.69	6e-08	Serine/threonine-protein kinase tousled-like 1-B OS Danio rerio GN tlk1b PE 2 SV 1
	sp\|Q91XS8\|ST17B_RAT	134	382	249	Gaps:57	54.99	371	32.84	18.63	1e-07	Serine/threonine-protein kinase 17B OS Rattus norvegicus GN Stk17b PE 1 SV 1
blastp_pdb	2a27_H	137	369	233	Gaps:56	57.63	321	33.51	18.38	3e-09	mol:protein length:321 Death-associated protein kinase 2
	2a27_G	137	369	233	Gaps:56	57.63	321	33.51	18.38	3e-09	mol:protein length:321 Death-associated protein kinase 2
	2a27_F	137	369	233	Gaps:56	57.63	321	33.51	18.38	3e-09	mol:protein length:321 Death-associated protein kinase 2
	2a27_E	137	369	233	Gaps:56	57.63	321	33.51	18.38	3e-09	mol:protein length:321 Death-associated protein kinase 2
	2a27_D	137	369	233	Gaps:56	57.63	321	33.51	18.38	3e-09	mol:protein length:321 Death-associated protein kinase 2
	2a27_C	137	369	233	Gaps:56	57.63	321	33.51	18.38	3e-09	mol:protein length:321 Death-associated protein kinase 2
	2a27_B	137	369	233	Gaps:56	57.63	321	33.51	18.38	3e-09	mol:protein length:321 Death-associated protein kinase 2
	2a27_A	137	369	233	Gaps:56	57.63	321	33.51	18.38	3e-09	mol:protein length:321 Death-associated protein kinase 2
	1zws_H	135	369	235	Gaps:56	64.93	288	33.16	18.72	3e-09	mol:protein length:288 DAP-kinase related protein 1
	1zws_G	135	369	235	Gaps:56	64.93	288	33.16	18.72	3e-09	mol:protein length:288 DAP-kinase related protein 1
rpsblast_cdd	gnl\|CDD\|128516	143	369	227	Gaps:53	72.13	244	31.25	14.77	6e-16	smart00220 S_TKc Serine/Threonine protein kinases catalytic domain. Phosphotransferases. Serine or threonine-specific kinase subfamily.
	gnl\|CDD\|143851	32	369	338	Gaps:110	96.92	260	29.76	18.25	1e-14	pfam00069 Pkinase Protein kinase domain.
	gnl\|CDD\|173623	35	369	335	Gaps:129	98.60	215	32.55	18.40	1e-14	cd00180 PKc Catalytic domain of Protein Kinases. Protein Kinases (PKs) catalytic (c) domain. PKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine or tyrosine residues on protein substrates. The PK family is part of a larger superfamily that includes the catalytic domains of RIO kinases aminoglycoside phosphotransferase choline kinase phosphoinositide 3-kinase (PI3K) and actin-fragmin kinase. PKs make up a large family of serine/threonine kinases protein tyrosine kinases (PTKs) and dual-specificity PKs that phosphorylate both serine/threonine and tyrosine residues of target proteins. Majority of protein phosphorylation about 95% occurs on serine residues while only 1% occurs on tyrosine residues. Protein phosphorylation is a mechanism by which a wide variety of cellular proteins such as enzymes and membrane channels are reversibly regulated in response to certain stimuli. PKs often function as components of signal transduction pathways in which one kinase activates a second kinase which in turn may act on other kinases this sequential action transmits a signal from the cell surface to target proteins which results in cellular responses. The PK family is one of the largest known protein families with more than 100 homologous yeast enzymes and 550 human proteins. A fraction of PK family members are pseudokinases that lack crucial residues for catalytic activity. The mutiplicity of kinases allows for specific regulation according to substrate tissue distribution and cellular localization. PKs regulate many cellular processes including proliferation division differentiation motility survival metabolism cell-cycle progression cytoskeletal rearrangement immunity and neuronal functions. Many kinases are implicated in the development of various human diseases including different types of cancer.
	gnl\|CDD\|173724	28	369	342	Gaps:103	98.08	260	29.02	19.61	6e-13	cd06606 STKc_MAPKKK Catalytic domain of the Protein Serine/Threonine Kinase Mitogen-Activated Protein Kinase Kinase Kinase. Serine/threonine kinases (STKs) mitogen-activated protein kinase (MAPK) kinase kinase (MAPKKK) subfamily catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The MAPKKK subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs protein tyrosine kinases RIO kinases aminoglycoside phosphotransferase choline kinase and phosphoinositide 3-kinase. MAPKKKs (MKKKs or MAP3Ks) are also called MAP/ERK kinase kinases (MEKKs) in some cases. They phosphorylate and activate MAPK kinases (MAPKKs or MKKs or MAP2Ks) which in turn phosphorylate and activate MAPKs during signaling cascades that are important in mediating cellular responses to extracellular signals. This subfamily is composed of the Apoptosis Signal-regulating Kinases ASK1 (or MAPKKK5) and ASK2 (or MAPKKK6) MEKK1 MEKK2 MEKK3 MEKK4 as well as plant and fungal MAPKKKs. Also included in this subfamily are the cell division control proteins Schizosaccharomyces pombe Cdc7 and Saccharomyces cerevisiae Cdc15.
	gnl\|CDD\|30861	143	467	325	Gaps:34	77.34	384	19.87	12.79	5e-11	COG0515 SPS1 Serine/threonine protein kinase [General function prediction only / Signal transduction mechanisms / Transcription / DNA replication recombination and repair].
	gnl\|CDD\|173757	123	369	247	Gaps:52	75.09	265	32.66	14.57	2e-09	cd08217 STKc_Nek2 Catalytic domain of the Protein Serine/Threonine Kinase Never In Mitosis gene A-related kinase 2. Serine/Threonine Kinases (STKs) Never In Mitosis gene A (NIMA)-related kinase 2 (Nek2) subfamily catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The Nek2 subfamily is one of a family of 11 different Neks (Nek1-11) that are involved in cell cycle control. The Nek family is part of a larger superfamily that includes the catalytic domains of other protein STKs protein tyrosine kinases RIO kinases aminoglycoside phosphotransferase choline kinase and phosphoinositide 3-kinase. The Nek2 subfamily includes Aspergillus nidulans NIMA kinase the founding member of the Nek family which was identified in a screen for cell cycle mutants prevented from entering mitosis. NIMA is essential for mitotic entry and progression through mitosis and its degradation is essential for mitotic exit. NIMA is involved in nuclear membrane fission. Vertebrate Nek2 is a cell cycle-regulated STK localized in centrosomes and kinetochores that regulates centrosome splitting at the G2/M phase. It also interacts with other mitotic kinases such as Polo-like kinase 1 and may play a role in spindle checkpoint. An increase in the expression of the human NEK2 gene is strongly associated with the progression of non-Hodgkin lymphoma.
	gnl\|CDD\|173765	205	369	165	Gaps:30	52.53	257	34.07	17.04	2e-09	cd08225 STKc_Nek5 Catalytic domain of the Protein Serine/Threonine Kinase Never In Mitosis gene A-related kinase 5. Serine/Threonine Kinases (STKs) Never In Mitosis gene A (NIMA)-related kinase 5 (Nek5) subfamily catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The Nek5 subfamily is one of a family of 11 different Neks (Nek1-11). The Nek family is part of a larger superfamily that includes the catalytic domains of other protein STKs protein tyrosine kinases RIO kinases aminoglycoside phosphotransferase choline kinase and phosphoinositide 3-kinase. Neks are involved in the regulation of downstream processes following the activation of Cdc2 and many of their functions are cell cycle-related. They play critical roles in microtubule dynamics during ciliogenesis and mitosis. The specific function of Nek5 is unknown.
	gnl\|CDD\|132957	143	369	227	Gaps:51	69.70	264	30.43	15.22	2e-09	cd06626 STKc_MEKK4 Catalytic domain of the Protein Serine/Threonine Kinase MAP/ERK kinase kinase 4. Serine/threonine kinases (STKs) MAP/ERK kinase kinase 4 (MEKK4) subfamily catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The MEKK4 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs protein tyrosine kinases RIO kinases aminoglycoside phosphotransferase choline kinase and phosphoinositide 3-kinase. MEKK4 is a mitogen-activated protein kinase (MAPK) kinase kinase (MAPKKK or MKKK or MAP3K) that phosphorylates and activates MAPK kinases (MAPKKs or MKKs or MAP2Ks) which in turn phosphorylate and activate MAPKs during signaling cascades that are important in mediating cellular responses to extracellular signals. MEKK4 activates the c-Jun N-terminal kinase (JNK) and p38 MAPK signaling pathways by directly activating their respective MAPKKs MKK4/MKK7 and MKK3/MKK6. JNK and p38 are collectively known as stress-activated MAPKs as they are activated in response to a variety of environmental stresses and pro-inflammatory cytokines. MEKK4 also plays roles in the re-polarization of the actin cytoskeleton in response to osmotic stress in the proper closure of the neural tube in cardiovascular development and in immune responses.
	gnl\|CDD\|132963	143	370	228	Gaps:56	68.22	258	34.09	15.91	5e-09	cd06632 STKc_MEKK1_plant Catalytic domain of the Protein Serine/Threonine Kinase Plant MAP/ERK kinase kinase 1. Serine/threonine kinases (STKs) plant MAP/ERK kinase kinase 1 (MEKK1)-like subfamily catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The plant MEKK1 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs protein tyrosine kinases RIO kinases aminoglycoside phosphotransferase choline kinase and phosphoinositide 3-kinase. This subfamily is composed of plant mitogen-activated protein kinase (MAPK) kinase kinases (MAPKKKs or MKKKs or MAP3Ks) including Arabidopsis thaliana MEKK1 and MAPKKK3. MEKK1 is a MAPKKK that phosphorylates and activates MAPK kinases (MAPKKs or MKKs or MAP2Ks) which in turn phosphorylate and activate MAPKs during signaling cascades that are important in mediating cellular responses to extracellular signals. Arabidopsis thaliana MEKK1 activates MPK4 a MAPK that regulates systemic acquired resistance. MEKK1 also participates in the regulation of temperature-sensitive and tissue-specific cell death.
	gnl\|CDD\|173672	139	309	171	Gaps:58	53.21	280	28.86	19.46	1e-08	cd05581 STKc_PDK1 Catalytic domain of the Protein Serine/Threonine Kinase Phosphoinositide-dependent kinase 1. Serine/Threonine Kinases (STKs) Phosphoinositide-dependent kinase 1 (PDK1) subfamily catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The PDK1 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs protein tyrosine kinases RIO kinases aminoglycoside phosphotransferase choline kinase and phosphoinositide 3-kinase (PI3K). PDK1 carries an N-terminal catalytic domain and a C-terminal pleckstrin homology (PH) domain that binds phosphoinositides. It phosphorylates the activation loop of AGC kinases that are regulated by PI3K such as PKB SGK and PKC among others and is crucial for their activation. Thus it contributes in regulating many processes including metabolism growth proliferation and survival. PDK1 also has the ability to autophosphorylate and is constitutively active in mammalian cells. PDK1 is essential for normal embryo development and is important in regulating cell volume.
rpsblast_kog	gnl\|CDD\|35419	28	392	365	Gaps:85	89.46	313	27.14	16.43	2e-14	KOG0198 KOG0198 KOG0198 MEKK and related serine/threonine protein kinases [Signal transduction mechanisms].
	gnl\|CDD\|35815	18	364	347	Gaps:93	60.61	429	29.23	17.31	2e-10	KOG0595 KOG0595 KOG0595 Serine/threonine-protein kinase involved in autophagy [Posttranslational modification protein turnover chaperones Intracellular trafficking secretion and vesicular transport Signal transduction mechanisms].
	gnl\|CDD\|35835	133	369	237	Gaps:51	41.26	475	32.65	18.37	1e-09	KOG0615 KOG0615 KOG0615 Serine/threonine protein kinase Chk2 and related proteins [Cell cycle control cell division chromosome partitioning].
	gnl\|CDD\|35818	109	374	266	Gaps:67	62.46	357	27.35	17.04	3e-09	KOG0598 KOG0598 KOG0598 Ribosomal protein S6 kinase and related proteins [General function prediction only Signal transduction mechanisms].
	gnl\|CDD\|39451	29	309	281	Gaps:80	28.55	732	30.14	16.75	5e-09	KOG4250 KOG4250 KOG4250 TANK binding protein kinase TBK1 [Signal transduction mechanisms].
	gnl\|CDD\|36366	209	368	160	Gaps:12	19.10	775	33.78	17.57	7e-09	KOG1151 KOG1151 KOG1151 Tousled-like protein kinase [Signal transduction mechanisms].
	gnl\|CDD\|35805	143	368	226	Gaps:43	31.77	576	29.51	20.77	9e-09	KOG0585 KOG0585 KOG0585 Ca2+/calmodulin-dependent protein kinase kinase beta and related serine/threonine protein kinases [Signal transduction mechanisms].
	gnl\|CDD\|35803	29	371	343	Gaps:94	69.46	370	26.85	20.23	2e-08	KOG0583 KOG0583 KOG0583 Serine/threonine protein kinase [Signal transduction mechanisms].
	gnl\|CDD\|35255	199	369	171	Gaps:25	38.22	382	28.77	15.07	8e-08	KOG0032 KOG0032 KOG0032 Ca2+/calmodulin-dependent protein kinase EF-Hand protein superfamily [Signal transduction mechanisms].
	gnl\|CDD\|37556	130	373	244	Gaps:41	71.19	302	27.91	19.53	9e-08	KOG2345 KOG2345 KOG2345 Serine/threonine protein kinase/TGF-beta stimulated factor [Transcription Lipid transport and metabolism Signal transduction mechanisms].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis

Hit name

RefStart

RefEnd

Hit length

Hit identity

Hit coverage

eValue

Hit description

RepeatMasker_ALL_Repbase

no results

TRF

no results

REPET

no results

sim4_Bot_allest

no results

sim4_Bot_allest4_clctg

no results

sim4_Ss_allest

no results

blastx_uniprot_sprot

no results

blastx_uniprot_trembl

no results

blastx_FNG30_prot

SS1G_07922.1

1052559

1053289

498

58.02

42.57

n/a

sclerotinia_sclerotiorum predicted protein translation 499 aa

sim4_B0510_genes

BC1G_07743.1

1050735

1051949

1215

99.59

100.00

n/a

BC1G_07742.1

1052550

1053307

663

99.09

99.85

n/a

BC1G_07743.1

1050735

1051949

1215

99.59

100.00

n/a

sim4_SS_genes

SS1G_07922.1

1052656

1053155

1497

87.83

7.68

n/a

blastn_b0510_supercontig

B0510_supercontig_1.44

1045937

1051979

273247

99.77

n/a

B0510_supercontig_1.44

1051902

1053421

273247

98.88

n/a

blastn_SS_supercontig

SS_supercontig_1.10

1052992

1053149

1547569

82.28

n/a

blat_oligo

botrytisP00042082

1051986

1052154

n/a

array_gene:BofuT4_P007050_1 . max_over_threshold:2.97 . min_over_threshold:0.43 . percent_hyb_over_threshold:39.28 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

botrytisP00042083

1051885

1051944

n/a

array_gene:BofuT4_P007050_1 . max_over_threshold:1.31 . min_over_threshold:0.32 . percent_hyb_over_threshold:7.14 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

botrytisP00042084

1051504

1051563

n/a

array_gene:BofuT4_P007050_1 . max_over_threshold:2.76 . min_over_threshold:0.5 . percent_hyb_over_threshold:42.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

botrytisP00042085

1051344

1051403

n/a

array_gene:BofuT4_P007050_1 . max_over_threshold:2.85 . min_over_threshold:0.47 . percent_hyb_over_threshold:46.42 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

botrytisP00042086

1051337

1051396

n/a

array_gene:BofuT4_P007050_1 . max_over_threshold:3.15 . min_over_threshold:0.41 . percent_hyb_over_threshold:32.14 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

botrytisP00042088

1051127

1051186

n/a

array_gene:BofuT4_P007050_1 . max_over_threshold:1.64 . min_over_threshold:0.46 . percent_hyb_over_threshold:7.14 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

botrytisP00042089

1050948

1051007

n/a

array_gene:BofuT4_P007050_1 . max_over_threshold:2.35 . min_over_threshold:0.46 . percent_hyb_over_threshold:39.28 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

botrytisP00042090

1050934

1050993

n/a

array_gene:BofuT4_P007050_1 . max_over_threshold:2.86 . min_over_threshold:0.49 . percent_hyb_over_threshold:32.14 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

botrytisP00042091

1050783

1050842

n/a

array_gene:BofuT4_P007050_1 . max_over_threshold:4.59 . min_over_threshold:0.83 . percent_hyb_over_threshold:92.85 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog