GRS - Genome Report System

Botrytis cinerea T4 GRS

Functional annotation

BofuT4P6000014001

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_12513	1	376	376	n/a	42.82	878	96.54	0.00	0.0	hypothetical protein K01684 galactonate dehydratase [EC:4.2.1.6]
	ssl:SS1G_10067	1	381	381	Gaps:6	100.00	387	89.66	2.33	0.0	hypothetical protein K01684 galactonate dehydratase [EC:4.2.1.6]
	pno:SNOG_09633	1	380	380	Gaps:14	80.44	455	76.78	9.56	1e-167	hypothetical protein K01684 galactonate dehydratase [EC:4.2.1.6]
	ani:AN4828.2	1	372	372	n/a	34.19	1088	69.62	9.95	1e-157	hypothetical protein K01684 galactonate dehydratase [EC:4.2.1.6]
	afm:AFUA_1G05520	1	381	381	Gaps:2	100.00	383	67.10	13.05	1e-154	mandelate racemase/muconate lactonizing enzyme family protein (EC:4.2.1.6) K01684 galactonate dehydratase [EC:4.2.1.6]
	nfi:NFIA_019250	1	381	381	Gaps:2	100.00	383	66.84	12.79	1e-154	mandelate racemase/muconate lactonizing enzyme family protein K01684 galactonate dehydratase [EC:4.2.1.6]
	aor:AO090001000258	1	381	381	Gaps:25	100.00	406	65.02	10.84	1e-153	L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily
	ang:An18g04330	1	381	381	Gaps:2	100.00	383	67.10	11.49	1e-153	hypothetical protein K01684 galactonate dehydratase [EC:4.2.1.6]
	act:ACLA_029150	1	381	381	Gaps:2	100.00	383	66.06	12.79	1e-152	mandelate racemase/muconate lactonizing enzyme family protein K01684 galactonate dehydratase [EC:4.2.1.6]
	afv:AFLA_050820	1	381	381	Gaps:13	100.00	372	67.74	12.90	1e-149	mandelate racemase/muconate lactonizing enzyme family protein K01684 galactonate dehydratase [EC:4.2.1.6]
blastp_uniprot_sprot	sp\|Q1R4P4\|DGOD_ECOUT	3	381	379	Gaps:4	99.74	382	46.19	17.85	1e-100	D-galactonate dehydratase OS Escherichia coli (strain UTI89 / UPEC) GN dgoD PE 3 SV 1
	sp\|Q0TB15\|DGOD_ECOL5	3	381	379	Gaps:4	99.74	382	45.93	17.85	2e-99	D-galactonate dehydratase OS Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN dgoD PE 3 SV 1
	sp\|A1AHM7\|DGOD_ECOK1	3	381	379	Gaps:4	99.74	382	45.93	17.85	2e-99	D-galactonate dehydratase OS Escherichia coli O1:K1 / APEC GN dgoD PE 3 SV 1
	sp\|B7N1Z6\|DGOD_ECO81	3	381	379	Gaps:4	99.74	382	45.93	17.85	2e-99	D-galactonate dehydratase OS Escherichia coli O81 (strain ED1a) GN dgoD PE 3 SV 1
	sp\|B7NQZ2\|DGOD_ECO7I	3	381	379	Gaps:4	99.74	382	45.93	17.85	2e-99	D-galactonate dehydratase OS Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN dgoD PE 3 SV 1
	sp\|B7MGB3\|DGOD_ECO45	3	381	379	Gaps:4	99.74	382	45.93	17.85	2e-99	D-galactonate dehydratase OS Escherichia coli O45:K1 (strain S88 / ExPEC) GN dgoD PE 3 SV 1
	sp\|B7LK34\|DGOD_ESCF3	3	381	379	Gaps:4	99.74	382	45.93	17.85	3e-99	D-galactonate dehydratase OS Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) GN dgoD PE 3 SV 1
	sp\|B6I3S5\|DGOD_ECOSE	3	381	379	Gaps:4	99.74	382	45.93	17.85	4e-99	D-galactonate dehydratase OS Escherichia coli (strain SE11) GN dgoD PE 3 SV 1
	sp\|B7NF09\|DGOD_ECOLU	3	381	379	Gaps:4	99.74	382	45.93	17.85	4e-99	D-galactonate dehydratase OS Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN dgoD PE 3 SV 1
	sp\|Q6BF17\|DGOD_ECOLI	3	381	379	Gaps:4	99.74	382	45.93	17.85	4e-99	D-galactonate dehydratase OS Escherichia coli (strain K12) GN dgoD PE 1 SV 1
blastp_pdb	2ox4_H	3	284	282	Gaps:35	78.16	403	33.65	18.10	1e-38	mol:protein length:403 Putative mandelate racemase
	2ox4_G	3	284	282	Gaps:35	78.16	403	33.65	18.10	1e-38	mol:protein length:403 Putative mandelate racemase
	2ox4_F	3	284	282	Gaps:35	78.16	403	33.65	18.10	1e-38	mol:protein length:403 Putative mandelate racemase
	2ox4_E	3	284	282	Gaps:35	78.16	403	33.65	18.10	1e-38	mol:protein length:403 Putative mandelate racemase
	2ox4_D	3	284	282	Gaps:35	78.16	403	33.65	18.10	1e-38	mol:protein length:403 Putative mandelate racemase
	2ox4_C	3	284	282	Gaps:35	78.16	403	33.65	18.10	1e-38	mol:protein length:403 Putative mandelate racemase
	2ox4_B	3	284	282	Gaps:35	78.16	403	33.65	18.10	1e-38	mol:protein length:403 Putative mandelate racemase
	2ox4_A	3	284	282	Gaps:35	78.16	403	33.65	18.10	1e-38	mol:protein length:403 Putative mandelate racemase
	2qq6_B	55	366	312	Gaps:41	82.68	410	30.97	21.53	7e-38	mol:protein length:410 Mandelate racemase/muconate lactonizing enzym
	2qq6_A	55	366	312	Gaps:41	82.68	410	30.97	21.53	7e-38	mol:protein length:410 Mandelate racemase/muconate lactonizing enzym
rpsblast_cdd	gnl\|CDD\|184455	3	381	379	Gaps:4	99.74	382	55.38	16.27	1e-164	PRK14017 PRK14017 galactonate dehydratase Provisional.
	gnl\|CDD\|48200	4	352	349	Gaps:4	99.72	352	58.40	16.81	1e-138	cd03325 D-galactonate_dehydratase D-galactonate dehydratase catalyses the dehydration of galactonate to 2-keto-3-deoxygalactnate (KDGal) as part of the D-galactonate nonphosphorolytic catabolic Entner-Doudoroff pathway. D-galactonate dehydratase belongs to the enolase superfamily of enzymes characterized by the presence of an enolate anion intermediate which is generated by abstraction of the alpha-proton of the carboxylate substrate by an active site residue and is stabilized by coordination to the essential Mg2+ ion..
	gnl\|CDD\|48191	14	350	337	Gaps:17	93.56	357	41.62	18.26	3e-86	cd03316 MR_like Mandelate racemase (MR)-like subfamily of the enolase superfamily. Enzymes of this subgroup share three conserved carboxylate ligands for the essential divalent metal ion (usually Mg2+) two aspartates and a glutamate and conserved catalytic residues a Lys-X-Lys motif and a conserved histidine-aspartate dyad. Members of the MR subgroup are mandelate racemase D-glucarate/L-idarate dehydratase (GlucD) D-altronate/D-mannonate dehydratase D-galactonate dehydratase (GalD) D-gluconate dehydratase (GlcD) and L-rhamnonate dehydratase (RhamD)..
	gnl\|CDD\|34556	15	365	351	Gaps:20	92.20	372	41.11	18.37	1e-75	COG4948 COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily [Cell envelope biogenesis outer membrane / General function prediction only].
	gnl\|CDD\|73347	4	366	363	Gaps:26	96.12	361	32.28	19.60	8e-57	cd03322 rpsA The starvation sensing protein RpsA from E.coli and its homologs are lactonizing enzymes whose putative targets are homoserine lactone (HSL)-derivative. They are part of the mandelate racemase (MR)-like subfamily of the enolase superfamily. Enzymes of this subfamily share three conserved carboxylate ligands for the essential divalent metal ion (usually Mg2+) two aspartates and a glutamate and catalytic residues a partially conserved Lys-X-Lys motif and a conserved histidine-aspartate dyad..
	gnl\|CDD\|73348	7	352	346	Gaps:22	99.71	341	31.76	20.59	5e-52	cd03327 MR_like_2 Mandelate racemase (MR)-like subfamily of the enolase superfamily subgroup 2. Enzymes of this subgroup share three conserved carboxylate ligands for the essential divalent metal ion (usually Mg2+) two aspartates and a glutamate and conserved catalytic residues a Lys-X-Lys motif and a conserved histidine-aspartate dyad. This subgroup's function is unknown..
	gnl\|CDD\|185032	19	354	336	Gaps:42	89.11	404	31.94	18.06	4e-51	PRK15072 PRK15072 bifunctional D-altronate/D-mannonate dehydratase Provisional.
	gnl\|CDD\|73188	83	308	226	Gaps:48	81.22	229	39.25	16.67	5e-32	cd00308 enolase_like Enolase-superfamily characterized by the presence of an enolate anion intermediate which is generated by abstraction of the alpha-proton of the carboxylate substrate by an active site residue and is stabilized by coordination to the essential Mg2+ ion. Enolase superfamily contains different enzymes like enolases glutarate- fucanate- and galactonate dehydratases o-succinylbenzoate synthase N-acylamino acid racemase L-alanine-DL-glutamate epimerase mandelate racemase muconate lactonizing enzyme and 3-methylaspartase..
	gnl\|CDD\|185337	17	283	267	Gaps:29	65.99	394	34.62	18.08	3e-31	PRK15440 PRK15440 L-rhamnonate dehydratase Provisional.
	gnl\|CDD\|48193	17	358	342	Gaps:35	91.78	365	28.96	16.72	4e-30	cd03318 MLE Muconate Lactonizing Enzyme (MLE) an homooctameric enzyme catalyses the conversion of cis cis-muconate (CCM) to muconolactone (ML) in the catechol branch of the beta-ketoadipate pathway. This pathway is used in soil microbes to breakdown lignin-derived aromatics catechol and protocatechuate to citric acid cycle intermediates. Some bacterial species are also capable of dehalogenating chloroaromatic compounds by the action of chloromuconate lactonizing enzymes (Cl-MLEs). MLEs are members of the enolase superfamily characterized by the presence of an enolate anion intermediate which is generated by abstraction of the alpha-proton of the carboxylate substrate by an active site residue and that is stabilized by coordination to the essential Mg2+ ion..
rpsblast_kog	no results

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
RepeatMasker_ALL_Repbase	no results
TRF	no results
REPET	no results
sim4_Bot_allest	YCKA_557878	254514	254586	73	100.00	100.00	n/a	Botrytis cinerea HortResearch libraries
	B2BC_045_120_F06	255076	255691	616	100.00	100.00	n/a	B0510 Spores suspension on bean library
	B2BC_045_120_H03	255076	255691	617	99.51	100.00	n/a	B0510 Spores suspension on bean library
	PD0ABA35YO02FM1	254435	255274	699	100.00	100.00	n/a	BT4 young mycelium library
	PD0ABA29YE18FM1	254434	255208	634	100.00	100.00	n/a	BT4 young mycelium library
sim4_Bot_allest4_clctg	CL_bt4_SuperContig_6_1_bt4ctg_0168_009.Contig1_v5	254434	255691	1118	99.91	100.00	n/a	all_lib_clone_nr:4
sim4_Ss_allest	no results
blastx_uniprot_sprot	Q6BF17	254488	255765	382	49.20	97.91	n/a	Galactonate dehydratase - Escherichia coli (strain K12)
blastx_uniprot_trembl	no results
blastx_FNG30_prot	SS1G_10067.1	254482	255765	387	93.54	100.00	n/a	sclerotinia_sclerotiorum hypothetical protein translation 388 aa
	SNU09633.1	254482	255765	381	78.48	100.00	n/a	Stagonospora nodorum hypothetical protein translation
	jgi:Triree:37302	254482	255765	381	74.28	100.00	n/a	Trichoderma reesei
	AO090001000258	254482	255765	406	69.37	97.29	n/a	Aspergillus oryzae L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily
	AN10599	254482	255765	381	71.65	100.00	n/a	Aspergillus nidulans AN10599.3 hypothetical protein translation 382 aa
	gi:70990904:ref:XP_750301.1:	254482	255765	383	68.67	100.00	n/a	Aspergillus fumigatus Af293 hypothetical protein Afu1g05520
	MGG_07935.5	254482	255765	383	63.09	99.74	n/a	magnaporthe_grisea hypothetical protein translation 384 aa
	FG03955.1	254482	255756	395	65.00	96.20	n/a	fusarium_graminearum conserved hypothetical protein 396 aa
	jgi:Phchr1:136861:e_gww2.7.346	254503	255765	410	59.09	91.22	n/a	Phanerochaete chrysosporium
	gi:58271358:ref:XP_572835.1	254708	255765	438	59.57	73.97	n/a	Cryptococcus neoformans var. neoformans JEC21 mandelate racemase/muconate lactonizing enzyme
	SS1G_10067.1	254482	255765	387	93.54	100.00	n/a	sclerotinia_sclerotiorum hypothetical protein translation 388 aa
	SNU09633.1	254482	255765	381	78.48	100.00	n/a	Stagonospora nodorum hypothetical protein translation
	jgi:Triree:37302	254482	255765	381	74.28	100.00	n/a	Trichoderma reesei
	AO090001000258	254482	255765	406	69.37	97.29	n/a	Aspergillus oryzae L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily
	AN10599	254482	255765	381	71.65	100.00	n/a	Aspergillus nidulans AN10599.3 hypothetical protein translation 382 aa
	gi:70990904:ref:XP_750301.1:	254482	255765	383	68.67	100.00	n/a	Aspergillus fumigatus Af293 hypothetical protein Afu1g05520
	MGG_07935.5	254482	255765	383	63.09	99.74	n/a	magnaporthe_grisea hypothetical protein translation 384 aa
	FG03955.1	254482	255756	395	65.00	96.20	n/a	fusarium_graminearum conserved hypothetical protein 396 aa
sim4_B0510_genes	BC1G_12513.1	254482	258969	2637	99.96	100.00	n/a	n/a
sim4_SS_genes	SS1G_10067.1	254482	255767	1164	86.68	100.00	n/a	n/a
sim4_SS_genes	SS1G_10067.1	254482	255767	1164	86.68	100.00	n/a	n/a
blastn_b0510_supercontig	B0510_supercontig_1.103	249572	267580	155081	99.88	n/a	n/a	n/a
blastn_SS_supercontig	SS_supercontig_1.15	255008	255767	1185750	85.26	n/a	n/a	n/a
	SS_supercontig_1.15	254862	254979	1185750	91.53	n/a	n/a	n/a
	SS_supercontig_1.15	254701	254808	1185750	91.67	n/a	n/a	n/a
	SS_supercontig_1.15	254476	254662	1185750	85.56	n/a	n/a	n/a
blat_oligo	botrytisP00046022	254604	254712	60	n/a	n/a	n/a	array_gene:BofuT4_P012370_1 . max_over_threshold:99.87 . min_over_threshold:4.11 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00046023	254735	254794	60	n/a	n/a	n/a	array_gene:BofuT4_P012370_1 . max_over_threshold:124.97 . min_over_threshold:8.24 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00046024	254908	254967	60	n/a	n/a	n/a	array_gene:BofuT4_P012370_1 . max_over_threshold:95.33 . min_over_threshold:3.69 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00046025	255057	255116	60	n/a	n/a	n/a	array_gene:BofuT4_P012370_1 . max_over_threshold:130.67 . min_over_threshold:5.63 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00046026	255145	255204	60	n/a	n/a	n/a	array_gene:BofuT4_P012370_1 . max_over_threshold:95.72 . min_over_threshold:4.22 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00046027	255270	255329	60	n/a	n/a	n/a	array_gene:BofuT4_P012370_1 . max_over_threshold:126.31 . min_over_threshold:5.94 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00046028	255439	255498	60	n/a	n/a	n/a	array_gene:BofuT4_P012370_1 . max_over_threshold:113.28 . min_over_threshold:6.51 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00046029	255458	255517	60	n/a	n/a	n/a	array_gene:BofuT4_P012370_1 . max_over_threshold:118.06 . min_over_threshold:4.3 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0
	botrytisP00046031	255641	255700	60	n/a	n/a	n/a	array_gene:BofuT4_P012370_1 . max_over_threshold:77.72 . min_over_threshold:3.71 . percent_hyb_over_threshold:100 . flag_cross_hyb_gene:0 . flag_cross_hyb_organism:0

Current manual annotation

Ortholog

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