GRS - Genome Report System

Botrytis cinerea B0510 GRS

Functional annotation

BC1G_15213.1

Gene Identifier

Domain/Motif (Interproscan results)

Localization / Targeting

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_15213	1	189	189	n/a	100.00	189	100.00	0.00	1e-106	GTP-binding protein sarA K07953 GTP-binding protein SAR1 [EC:3.6.5.-]
	ssl:SS1G_07320	1	189	189	n/a	100.00	189	99.47	0.53	1e-106	GTP-binding protein SARA K07953 GTP-binding protein SAR1 [EC:3.6.5.-]
	tml:GSTUM_00012085001	1	189	189	n/a	100.00	189	91.53	5.82	1e-100	hypothetical protein K07953 GTP-binding protein SAR1 [EC:3.6.5.-]
	ang:An01g04040	1	189	189	n/a	100.00	189	92.06	5.82	1e-100	sarA secretion-associated GTP-binding protein sarA-Aspergillus niger K07953 GTP-binding protein SAR1 [EC:3.6.5.-]
	afv:AFLA_029760	1	189	189	n/a	100.00	189	91.01	6.88	1e-100	small monomeric GTPase SarA putative K07953 GTP-binding protein SAR1 [EC:3.6.5.-]
	aor:AO090003000842	1	189	189	n/a	100.00	189	91.01	6.88	1e-100	vesicle coat complex COPII GTPase subunit SAR1 K07953 GTP-binding protein SAR1 [EC:3.6.5.-]
	act:ACLA_029700	1	189	189	n/a	100.00	189	91.53	5.82	1e-99	small monomeric GTPase SarA putative K07953 GTP-binding protein SAR1 [EC:3.6.5.-]
	nfi:NFIA_019790	1	189	189	n/a	100.00	189	91.01	6.35	2e-99	small monomeric GTPase SarA putative K07953 GTP-binding protein SAR1 [EC:3.6.5.-]
	afm:AFUA_1G04940	1	189	189	n/a	100.00	189	91.01	6.35	3e-99	small monomeric GTPase SarA K07953 GTP-binding protein SAR1 [EC:3.6.5.-]
	mgr:MGG_06362	1	189	189	n/a	100.00	189	89.95	8.99	7e-99	MG06362.4 GTP-binding protein SAR1 K07953 GTP-binding protein SAR1 [EC:3.6.5.-]
blastp_uniprot_sprot	sp\|P0C951\|SAR1_ASPNC	1	189	189	n/a	100.00	189	92.06	5.82	1e-101	Small COPII coat GTPase SAR1 OS Aspergillus niger (strain CBS 513.88 / FGSC A1513) GN sar1 PE 3 SV 1
	sp\|Q877B9\|SAR1_ASPOR	1	189	189	n/a	100.00	189	91.01	6.88	1e-100	Small COPII coat GTPase sar1 OS Aspergillus oryzae GN sar1 PE 3 SV 1
	sp\|A1CRG9\|SAR1_ASPCL	1	189	189	n/a	100.00	189	91.53	5.82	1e-100	Small COPII coat GTPase sar1 OS Aspergillus clavatus GN sar1 PE 3 SV 1
	sp\|A1D4D1\|SAR1_NEOFI	1	189	189	n/a	100.00	189	91.01	6.35	1e-100	Small COPII coat GTPase sar1 OS Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) GN sar1 PE 3 SV 1
	sp\|Q4WJS7\|SAR1_ASPFU	1	189	189	n/a	100.00	189	91.01	6.35	1e-100	Small COPII coat GTPase sar1 OS Aspergillus fumigatus GN sar1 PE 3 SV 1
	sp\|Q0CUN7\|SAR1_ASPTN	1	189	189	n/a	100.00	189	91.01	6.88	1e-100	Small COPII coat GTPase sar1 OS Aspergillus terreus (strain NIH 2624 / FGSC A1156) GN sar1 PE 3 SV 1
	sp\|Q5EMZ6\|SAR1_MAGGR	1	189	189	n/a	100.00	189	89.95	8.99	1e-100	Small COPII coat GTPase SAR1 OS Magnaporthe grisea GN SAR1 PE 2 SV 1
	sp\|Q5BGB9\|SAR1_EMENI	1	189	189	n/a	100.00	189	90.48	6.35	1e-99	Small COPII coat GTPase sar1 OS Emericella nidulans GN sar1 PE 3 SV 1
	sp\|P0C950\|SAR1_ASPNG	1	189	189	n/a	100.00	189	91.01	5.82	3e-99	Small COPII coat GTPase SAR1 OS Aspergillus niger GN sar1 PE 3 SV 1
	sp\|Q2HA55\|SAR1_CHAGB	1	188	188	n/a	98.95	190	87.77	10.64	3e-98	Small COPII coat GTPase SAR1 OS Chaetomium globosum GN SAR1 PE 3 SV 2
blastp_pdb	1m2o_D	3	189	187	Gaps:2	97.37	190	73.51	10.27	7e-76	mol:protein length:190 GTP-binding protein SAR1
	1m2o_B	3	189	187	Gaps:2	97.37	190	73.51	10.27	7e-76	mol:protein length:190 GTP-binding protein SAR1
	2qtv_B	20	188	169	Gaps:2	100.00	167	74.85	10.18	1e-69	mol:protein length:167 Small COPII coat GTPase SAR1
	1f6b_B	2	189	188	Gaps:3	96.46	198	64.40	13.09	2e-67	mol:protein length:198 SAR1
	1f6b_A	2	189	188	Gaps:3	96.46	198	64.40	13.09	2e-67	mol:protein length:198 SAR1
	2gao_B	5	189	185	Gaps:3	90.38	208	65.96	11.70	3e-67	mol:protein length:208 GTP-binding protein SAR1a
	2gao_A	5	189	185	Gaps:3	90.38	208	65.96	11.70	3e-67	mol:protein length:208 GTP-binding protein SAR1a
	2fmx_B	7	189	183	Gaps:3	95.38	195	65.59	12.90	1e-66	mol:protein length:195 GTP-binding protein SAR1b
	2fmx_A	7	189	183	Gaps:3	95.38	195	65.59	12.90	1e-66	mol:protein length:195 GTP-binding protein SAR1b
	2fa9_B	7	189	183	Gaps:3	98.41	189	65.05	12.90	1e-65	mol:protein length:189 GTP-binding protein SAR1b
rpsblast_cdd	gnl\|CDD\|133255	2	189	188	Gaps:2	100.00	190	72.63	10.53	6e-98	cd00879 Sar1 Sar1 subfamily. Sar1 is an essential component of COPII vesicle coats involved in export of cargo from the ER. The GTPase activity of Sar1 functions as a molecular switch to control protein-protein and protein-lipid interactions that direct vesicle budding from the ER. Activation of the GDP to the GTP-bound form of Sar1 involves the membrane-associated guanine nucleotide exchange factor (GEF) Sec12. Sar1 is unlike all Ras superfamily GTPases that use either myristoyl or prenyl groups to direct membrane association and function in that Sar1 lacks such modification. Instead Sar1 contains a unique nine-amino-acid N-terminal extension. This extension contains an evolutionarily conserved cluster of bulky hydrophobic amino acids referred to as the Sar1-N-terminal activation recruitment (STAR) motif. The STAR motif mediates the recruitment of Sar1 to ER membranes and facilitates its interaction with mammalian Sec12 GEF leading to activation.
	gnl\|CDD\|128475	4	189	186	Gaps:2	100.00	184	77.72	8.15	7e-91	smart00178 SAR Sar1p-like members of the Ras-family of small GTPases. Yeast SAR1 is an essential gene required for transport of secretory proteins from the endoplasmic reticulum to the Golgi apparatus.
	gnl\|CDD\|143815	6	189	184	Gaps:10	100.00	174	45.98	16.67	4e-67	pfam00025 Arf ADP-ribosylation factor family. Pfam combines a number of different Prosite families together.
	gnl\|CDD\|133254	22	188	167	Gaps:9	100.00	158	44.94	13.92	4e-45	cd00878 Arf_Arl Arf (ADP-ribosylation factor)/Arl (Arf-like) small GTPases. Arf proteins are activators of phospholipase D isoforms. Unlike Ras proteins they lack cysteine residues at their C-termini and therefore are unlikely to be prenylated. Arfs are N-terminally myristoylated. Members of the Arf family are regulators of vesicle formation in intracellular traffic that interact reversibly with membranes of the secretory and endocytic compartments in a GTP-dependent manner. They depart from other small GTP-binding proteins by a unique structural device interswitch toggle that implements front-back communication from N-terminus to the nucleotide binding site. Arf-like (Arl) proteins are close relatives of the Arf but only Arl1 has been shown to function in membrane traffic like the Arf proteins. Arl2 has an unrelated function in the folding of native tubulin and Arl4 may function in the nucleus. Most other Arf family proteins are so far relatively poorly characterized. Thus despite their significant sequence homologies Arf family proteins may regulate unrelated functions.
	gnl\|CDD\|133357	23	187	165	Gaps:13	98.77	162	36.88	21.88	2e-28	cd04157 Arl6 Arl6 subfamily. Arl6 (Arf-like 6) forms a subfamily of the Arf family of small GTPases. Arl6 expression is limited to the brain and kidney in adult mice but it is expressed in the neural plate and somites during embryogenesis suggesting a possible role for Arl6 in early development. Arl6 is also believed to have a role in cilia or flagella function. Several proteins have been identified that bind Arl6 including Arl6 interacting protein (Arl6ip) and SEC61beta a subunit of the heterotrimeric conducting channel SEC61p. Based on Arl6 binding to these effectors Arl6 is also proposed to play a role in protein transport membrane trafficking or cell signaling during hematopoietic maturation. At least three specific homozygous Arl6 mutations in humans have been found to cause Bardet-Biedl syndrome a disorder characterized by obesity retinopathy polydactyly renal and cardiac malformations learning disabilities and hypogenitalism. Older literature suggests that Arl6 is a part of the Arl4/Arl7 subfamily but analyses based on more recent sequence data place Arl6 in its own subfamily.
	gnl\|CDD\|133353	18	187	170	Gaps:9	92.53	174	32.30	19.25	1e-27	cd04153 Arl5_Arl8 Arl5/Arl8 subfamily. Arl5 (Arf-like 5) and Arl8 like Arl4 and Arl7 are localized to the nucleus and nucleolus. Arl5 is developmentally regulated during embryogenesis in mice. Human Arl5 interacts with the heterochromatin protein 1-alpha (HP1alpha) a nonhistone chromosomal protein that is associated with heterochromatin and telomeres and prevents telomere fusion. Arl5 may also play a role in embryonic nuclear dynamics and/or signaling cascades. Arl8 was identified from a fetal cartilage cDNA library. It is found in brain heart lung cartilage and kidney. No function has been assigned for Arl8 to date.
	gnl\|CDD\|133351	22	187	166	Gaps:9	99.37	158	37.58	17.83	2e-27	cd04151 Arl1 Arl1 subfamily. Arl1 (Arf-like 1) localizes to the Golgi complex where it is believed to recruit effector proteins to the trans-Golgi network. Like most members of the Arf family Arl1 is myristoylated at its N-terminal helix and mutation of the myristoylation site disrupts Golgi targeting. In humans the Golgi-localized proteins golgin-97 and golgin-245 have been identified as Arl1 effectors. Golgins are large coiled-coil proteins found in the Golgi and these golgins contain a C-terminal GRIP domain which is the site of Arl1 binding. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. Arl1 is not required for exocytosis but appears necessary for trafficking from the endosomes to the Golgi. In Drosophila zygotes mutation of Arl1 is lethal and in the host-bloodstream form of Trypanosoma brucei Arl1 is essential for viability.
	gnl\|CDD\|133355	14	185	172	Gaps:15	97.69	173	33.14	18.34	3e-27	cd04155 Arl3 Arl3 subfamily. Arl3 (Arf-like 3) is an Arf family protein that differs from most Arf family members in the N-terminal extension. In is inactive GDP-bound form the N-terminal extension forms an elongated loop that is hydrophobically anchored into the membrane surface however it has been proposed that this region might form a helix in the GTP-bound form. The delta subunit of the rod-specific cyclic GMP phosphodiesterase type 6 (PDEdelta) is an Arl3 effector. Arl3 binds microtubules in a regulated manner to alter specific aspects of cytokinesis via interactions with retinitis pigmentosa 2 (RP2). It has been proposed that RP2 functions in concert with Arl3 to link the cell membrane and the cytoskeleton in photoreceptors as part of the cell signaling or vesicular transport machinery. In mice the absence of Arl3 is associated with abnormal epithelial cell proliferation and cyst formation.
	gnl\|CDD\|133349	18	187	170	Gaps:9	95.83	168	35.40	19.25	7e-26	cd04149 Arf6 Arf6 subfamily. Arf6 (ADP ribosylation factor 6) proteins localize to the plasma membrane where they perform a wide variety of functions. In its active GTP-bound form Arf6 is involved in cell spreading Rac-induced formation of plasma membrane ruffles cell migration wound healing and Fc-mediated phagocytosis. Arf6 appears to change the actin structure at the plasma membrane by activating Rac a Rho family protein involved in membrane ruffling. Arf6 is required for and enhances Rac formation of ruffles. Arf6 can regulate dendritic branching in hippocampal neurons and in yeast it localizes to the growing bud where it plays a role in polarized growth and bud site selection. In leukocytes Arf6 is required for chemokine-stimulated migration across endothelial cells. Arf6 also plays a role in down-regulation of beta2-adrenergic receptors and luteinizing hormone receptors by facilitating the release of sequestered arrestin to allow endocytosis. Arf6 is believed to function at multiple sites on the plasma membrane through interaction with a specific set of GEFs GAPs and effectors. Arf6 has been implicated in breast cancer and melanoma cell invasion and in actin remodelling at the invasion site of Chlamydia infection.
	gnl\|CDD\|133354	19	185	167	Gaps:9	91.33	173	36.71	17.72	1e-25	cd04154 Arl2 Arl2 subfamily. Arl2 (Arf-like 2) GTPases are members of the Arf family that bind GDP and GTP with very low affinity. Unlike most Arf family proteins Arl2 is not myristoylated at its N-terminal helix. The protein PDE-delta first identified in photoreceptor rod cells binds specifically to Arl2 and is structurally very similar to RhoGDI. Despite the high structural similarity between Arl2 and Rho proteins and between PDE-delta and RhoGDI the interactions between the GTPases and their effectors are very different. In its GTP bound form Arl2 interacts with the protein Binder of Arl2 (BART) and the complex is believed to play a role in mitochondrial adenine nucleotide transport. In its GDP bound form Arl2 interacts with tubulin- folding Cofactor D this interaction is believed to play a role in regulation of microtubule dynamics that impact the cytoskeleton cell division and cytokinesis.
rpsblast_kog	gnl\|CDD\|35300	1	189	189	Gaps:3	99.48	193	68.23	9.38	1e-81	KOG0077 KOG0077 KOG0077 Vesicle coat complex COPII GTPase subunit SAR1 [Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35293	11	187	177	Gaps:9	92.82	181	36.90	18.45	9e-31	KOG0070 KOG0070 KOG0070 GTP-binding ADP-ribosylation factor Arf1 [Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35294	9	187	179	Gaps:9	94.44	180	35.29	18.24	2e-26	KOG0071 KOG0071 KOG0071 GTP-binding ADP-ribosylation factor Arf6 (dArf3) [Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35296	19	185	167	Gaps:8	85.95	185	35.85	15.72	4e-24	KOG0073 KOG0073 KOG0073 GTP-binding ADP-ribosylation factor-like protein ARL2 [Intracellular trafficking secretion and vesicular transport Cytoskeleton].
	gnl\|CDD\|35299	24	185	162	Gaps:16	82.23	197	35.19	15.43	2e-22	KOG0076 KOG0076 KOG0076 GTP-binding ADP-ribosylation factor-like protein yARL3 [Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35298	3	154	152	Gaps:6	79.57	186	34.46	22.97	8e-21	KOG0075 KOG0075 KOG0075 GTP-binding ADP-ribosylation factor-like protein [General function prediction only].
	gnl\|CDD\|35295	18	187	170	Gaps:9	88.46	182	31.06	21.74	2e-20	KOG0072 KOG0072 KOG0072 GTP-binding ADP-ribosylation factor-like protein ARL1 [Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35297	1	185	185	Gaps:13	94.05	185	31.61	18.39	4e-20	KOG0074 KOG0074 KOG0074 GTP-binding ADP-ribosylation factor-like protein ARL3 [General function prediction only].
	gnl\|CDD\|35313	23	148	126	Gaps:10	57.14	238	29.41	11.76	1e-11	KOG0090 KOG0090 KOG0090 Signal recognition particle receptor beta subunit (small G protein superfamily) [Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35305	49	132	84	Gaps:10	26.55	354	23.40	20.21	4e-07	KOG0082 KOG0082 KOG0082 G-protein alpha subunit (small G protein superfamily) [Cell cycle control cell division chromosome partitioning Signal transduction mechanisms].

Other analysis

Functional Gbrowse

Ortholog