GRS - Genome Report System

Botrytis cinerea B0510 GRS

Functional annotation

BC1G_09632.1

Gene Identifier

Domain/Motif (Interproscan results)

Localization / Targeting

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_09632	1	282	282	n/a	100.00	282	100.00	0.00	1e-164	hypothetical protein
	ssl:SS1G_14415	14	281	268	Gaps:2	98.15	271	83.08	7.52	1e-126	hypothetical protein
	cim:CIMG_07094	9	281	273	Gaps:17	98.59	284	56.07	10.36	9e-79	hypothetical protein
	ang:An01g07510	9	281	273	Gaps:9	98.54	274	58.52	12.96	2e-78	hypothetical protein
	afv:AFLA_050530	9	281	273	Gaps:9	98.54	274	57.41	12.59	4e-78	nitrilase putitive
	afm:AFUA_4G12240	10	281	272	Gaps:9	98.18	274	55.76	13.38	6e-76	nitrilase putitive
	ncr:NCU05757	10	281	272	Gaps:11	98.20	278	55.31	13.55	3e-75	similar to nitrilase
	aor:AO090009000425	25	281	257	Gaps:9	97.69	260	57.87	12.20	4e-73	carbon-nitrogen hydrolase
	pan:PODANSg765	9	281	273	Gaps:11	89.74	312	52.86	13.21	2e-71	hypothetical protein
	act:ACLA_051240	25	281	257	Gaps:9	97.69	260	55.51	14.57	4e-71	hydrolase carbon-nitrogen family protein
blastp_uniprot_sprot	sp\|O94660\|NIT2_SCHPO	9	281	273	Gaps:9	98.55	276	44.12	18.38	1e-57	Probable hydrolase nit2 OS Schizosaccharomyces pombe GN nit2 PE 2 SV 1
	sp\|P47016\|NIT2_YEAST	11	281	271	Gaps:42	97.39	307	39.80	19.40	6e-51	Probable hydrolase NIT2 OS Saccharomyces cerevisiae GN NIT2 PE 2 SV 1
	sp\|O76463\|NFT1_CAEEL	10	281	272	Gaps:16	61.36	440	39.63	20.37	6e-49	Nitrilase and fragile histidine triad fusion protein NitFhit OS Caenorhabditis elegans GN nft-1 PE 1 SV 1
	sp\|Q86X76\|NIT1_HUMAN	10	281	272	Gaps:18	83.18	327	42.65	14.71	2e-48	Nitrilase homolog 1 OS Homo sapiens GN NIT1 PE 1 SV 2
	sp\|Q32LH4\|NIT1_BOVIN	10	281	272	Gaps:18	82.93	328	41.91	16.91	7e-48	Nitrilase homolog 1 OS Bos taurus GN NIT1 PE 2 SV 1
	sp\|Q7TQ94\|NIT1_RAT	10	281	272	Gaps:20	93.15	292	42.65	17.28	1e-47	Nitrilase homolog 1 OS Rattus norvegicus GN Nit1 PE 2 SV 1
	sp\|Q8VDK1\|NIT1_MOUSE	10	281	272	Gaps:20	84.21	323	42.65	16.91	3e-47	Nitrilase homolog 1 OS Mus musculus GN Nit1 PE 2 SV 1
	sp\|O76464\|NFT1_DROME	11	281	271	Gaps:15	57.83	460	40.60	16.92	2e-46	Nitrilase and fragile histidine triad fusion protein NitFhit OS Drosophila melanogaster GN NitFhit PE 1 SV 1
	sp\|Q557J5\|NIT1_DICDI	6	275	270	Gaps:14	93.47	291	35.66	19.85	3e-39	Nitrilase homolog 1 OS Dictyostelium discoideum GN nit1-1 PE 3 SV 1
	sp\|P55175\|Y601_SYNY3	10	275	266	Gaps:18	96.32	272	35.88	19.47	2e-32	UPF0012 hydrolase sll0601 OS Synechocystis sp. (strain PCC 6803) GN sll0601 PE 3 SV 1
blastp_pdb	1ems_B	10	281	272	Gaps:16	61.36	440	39.63	20.37	1e-49	mol:protein length:440 NIT-FRAGILE HISTIDINE TRIAD FUSION PROTEIN
	1ems_A	10	281	272	Gaps:16	61.36	440	39.63	20.37	1e-49	mol:protein length:440 NIT-FRAGILE HISTIDINE TRIAD FUSION PROTEIN
	2w1v_B	11	281	271	Gaps:21	95.65	276	33.33	18.56	8e-28	mol:protein length:276 NITRILASE HOMOLOG 2
	2w1v_A	11	281	271	Gaps:21	95.65	276	33.33	18.56	8e-28	mol:protein length:276 NITRILASE HOMOLOG 2
	1f89_B	11	281	271	Gaps:18	93.81	291	32.60	18.32	2e-27	mol:protein length:291 32.5 KDA PROTEIN YLR351C
	1f89_A	11	281	271	Gaps:18	93.81	291	32.60	18.32	2e-27	mol:protein length:291 32.5 KDA PROTEIN YLR351C
	1j31_D	21	262	242	Gaps:19	85.11	262	29.15	19.73	2e-14	mol:protein length:262 Hypothetical protein PH0642
	1j31_C	21	262	242	Gaps:19	85.11	262	29.15	19.73	2e-14	mol:protein length:262 Hypothetical protein PH0642
	1j31_B	21	262	242	Gaps:19	85.11	262	29.15	19.73	2e-14	mol:protein length:262 Hypothetical protein PH0642
	1j31_A	21	262	242	Gaps:19	85.11	262	29.15	19.73	2e-14	mol:protein length:262 Hypothetical protein PH0642
rpsblast_cdd	gnl\|CDD\|143596	10	275	266	Gaps:13	98.49	265	43.30	17.24	2e-90	cd07572 nit Nit1 Nit 2 and related proteins and the Nit1-like domain of NitFhit (class 10 nitrilases). This subgroup includes mammalian Nit1 and Nit2 the Nit1-like domain of the invertebrate NitFhit and various uncharacterized bacterial and archaeal Nit-like proteins. Nit1 and Nit2 are candidate tumor suppressor proteins. In NitFhit the Nit1-like domain is encoded as a fusion protein with the non-homologous tumor suppressor fragile histidine triad (Fhit). Mammalian Nit1 and Fhit may affect distinct signal pathways and both may participate in DNA damage-induced apoptosis. Nit1 is a negative regulator in T cells. Overexpression of Nit2 in HeLa cells leads to a suppression of cell growth through cell cycle arrest in G2. These Nit proteins and the Nit1-like domain of NitFhit belong to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) this subgroup corresponds to class 10.
	gnl\|CDD\|166439	11	274	264	Gaps:13	91.90	284	43.30	14.56	1e-66	PLN02798 PLN02798 nitrilase.
	gnl\|CDD\|143605	11	276	266	Gaps:20	98.82	255	38.89	14.29	4e-57	cd07581 nitrilase_3 Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases). The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
	gnl\|CDD\|30737	11	275	265	Gaps:18	93.07	274	36.08	16.08	3e-49	COG0388 COG0388 Predicted amidohydrolase [General function prediction only].
	gnl\|CDD\|143607	11	276	266	Gaps:19	98.42	253	36.14	16.06	1e-48	cd07583 nitrilase_5 Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases). The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
	gnl\|CDD\|143587	11	275	265	Gaps:22	98.42	253	38.96	16.06	2e-46	cd07197 nitrilase Nitrilase superfamily including nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes. This superfamily (also known as the C-N hydrolase superfamily) contains hydrolases that break carbon-nitrogen bonds it includes nitrilases cyanide dihydratases aliphatic amidases N-terminal amidases beta-ureidopropionases biotinidases pantotheinase N-carbamyl-D-amino acid amidohydrolases the glutaminase domain of glutamine-dependent NAD+ synthetase apolipoprotein N-acyltransferases and N-carbamoylputrescine amidohydrolases among others. These enzymes depend on a Glu-Lys-Cys catalytic triad and work through a thiol acylenzyme intermediate. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer. These oligomers include dimers tetramers hexamers octamers tetradecamers octadecamers as well as variable length helical arrangements and homo-oligomeric spirals. These proteins have roles in vitamin and co-enzyme metabolism in detoxifying small molecules in the synthesis of signaling molecules and in the post-translational modification of proteins. They are used industrially as biocatalysts in the fine chemical and pharmaceutical industry in cyanide remediation and in the treatment of toxic effluent. This superfamily has been classified previously in the literature based on global and structure-based sequence analysis into thirteen different enzyme classes (referred to as 1-13). This hierarchy includes those thirteen classes and a few additional subfamilies. A putative distant relative the plasmid-borne TraB family has not been included in the hierarchy.
	gnl\|CDD\|144405	10	186	177	Gaps:7	100.00	172	30.81	16.28	2e-26	pfam00795 CN_hydrolase Carbon-nitrogen hydrolase. This family contains hydrolases that break carbon-nitrogen bonds. The family includes: Nitrilase EC:3.5.5.1 Aliphatic amidase EC:3.5.1.4 Biotidinase EC:3.5.1.12 Beta-ureidopropionase EC:3.5.1.6. Nitrilase-related proteins generally have a conserved E-K-C catalytic triad and are multimeric alpha-beta-beta-alpha sandwich proteins.
	gnl\|CDD\|143599	21	282	262	Gaps:51	94.84	252	36.40	16.32	1e-25	cd07575 Xc-1258_like Xanthomonas campestris XC1258 and related proteins members of the nitrilase superfamily (putative class 13 nitrilases). Uncharacterized subgroup belonging to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup either represents a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer. XC1258 is a homotetramer.
	gnl\|CDD\|143600	11	276	266	Gaps:24	97.64	254	33.47	15.73	3e-24	cd07576 R-amidase_like Pseudomonas sp. MCI3434 R-amidase and related proteins (putative class 13 nitrilases). Pseudomonas sp. MCI3434 R-amidase hydrolyzes (R S)-piperazine-2-tert-butylcarboxamide to form (R)-piperazine-2-carboxylic acid. It does so with strict R-stereoselectively. Its preferred substrates are carboxamide compounds which have the amino or imino group connected to their beta- or gamma-carbon. This subgroup belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) class 13 represents proteins that at the time were difficult to place in a distinct similarity group. It has been suggested that this subgroup represents a new class. Members of the nitrilase superfamily generally form homomeric complexes the basic building block of which is a homodimer. Native R-amidase however appears to be a monomer.
	gnl\|CDD\|143608	9	276	268	Gaps:24	98.45	258	27.17	21.26	8e-21	cd07584 nitrilase_6 Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases). The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer.
rpsblast_kog	gnl\|CDD\|36025	7	281	275	Gaps:14	93.22	295	46.91	17.09	3e-74	KOG0807 KOG0807 KOG0807 Carbon-nitrogen hydrolase [Amino acid transport and metabolism].
rpsblast_kog	gnl\|CDD\|36024	9	281	273	Gaps:18	92.95	298	28.52	17.69	7e-29	KOG0806 KOG0806 KOG0806 Carbon-nitrogen hydrolase [Amino acid transport and metabolism].

Other analysis

Functional Gbrowse

Ortholog