GRS - Genome Report System

Botrytis cinerea B0510 GRS

Functional annotation

BC1G_06596.1

Gene Identifier

Domain/Motif (Interproscan results)

Localization / Targeting

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	bfu:BC1G_06596	1	527	527	n/a	100.00	527	94.12	0.00	0.0	hypothetical protein K00928 aspartate kinase [EC:2.7.2.4]
	ssl:SS1G_00574	1	527	527	n/a	100.00	527	88.80	3.80	0.0	similar to aspartate kinase K00928 aspartate kinase [EC:2.7.2.4]
	ncr:NCU04118	21	527	507	Gaps:12	99.04	522	59.19	14.89	1e-176	aspartokinase K00928 aspartate kinase [EC:2.7.2.4]
	pan:PODANSg8818	20	527	508	Gaps:9	97.86	514	60.44	14.91	1e-173	hypothetical protein K00928 aspartate kinase [EC:2.7.2.4]
	mgr:MGG_11805	24	495	472	Gaps:5	99.37	474	61.15	14.65	1e-165	MG06879.4 hypothetical protein K00928 aspartate kinase [EC:2.7.2.4]
	fgr:FG07421.1	20	527	508	Gaps:30	99.59	486	61.36	13.64	1e-160	hypothetical protein K00928 aspartate kinase [EC:2.7.2.4]
	ani:AN8859.2	8	527	520	Gaps:38	99.81	525	54.77	15.08	1e-150	hypothetical protein K00928 aspartate kinase [EC:2.7.2.4]
	ang:An17g02280	18	527	510	Gaps:37	96.95	525	55.21	15.32	1e-150	hypothetical protein K00928 aspartate kinase [EC:2.7.2.4]
	act:ACLA_008890	1	527	527	Gaps:42	100.00	521	54.32	16.51	1e-149	aspartokinase K00928 aspartate kinase [EC:2.7.2.4]
	aor:AO090009000702	18	527	510	Gaps:35	97.33	525	55.38	15.66	1e-148	aspartate kinase K00928 aspartate kinase [EC:2.7.2.4]
blastp_uniprot_sprot	sp\|O60163\|AK_SCHPO	24	523	500	Gaps:27	93.83	519	48.87	15.40	1e-125	Probable aspartokinase OS Schizosaccharomyces pombe GN SPBC19F5.04 PE 1 SV 1
	sp\|P10869\|AK_YEAST	26	524	499	Gaps:32	93.55	527	48.28	17.04	1e-118	Aspartokinase OS Saccharomyces cerevisiae GN HOM3 PE 1 SV 2
	sp\|O23653\|AK2_ARATH	161	523	363	Gaps:31	64.71	544	30.97	21.88	1e-40	Aspartokinase 2 chloroplastic OS Arabidopsis thaliana GN AK2 PE 1 SV 2
	sp\|Q57991\|AK_METJA	32	519	488	Gaps:67	97.46	473	30.15	23.64	5e-40	Probable aspartokinase OS Methanocaldococcus jannaschii GN MJ0571 PE 1 SV 1
	sp\|Q9S702\|AK3_ARATH	32	523	492	Gaps:62	81.22	559	31.06	20.93	2e-39	Aspartokinase 3 chloroplastic OS Arabidopsis thaliana GN AK3 PE 1 SV 1
	sp\|Q9LYU8\|AK1_ARATH	113	523	411	Gaps:43	68.54	569	31.54	20.77	3e-39	Aspartokinase 1 chloroplastic OS Arabidopsis thaliana GN AK1 PE 1 SV 1
	sp\|P44505\|AKH_HAEIN	174	524	351	Gaps:41	41.23	815	33.04	19.05	3e-29	Bifunctional aspartokinase/homoserine dehydrogenase OS Haemophilus influenzae GN thrA PE 3 SV 1
	sp\|P08660\|AK3_ECOLI	31	523	493	Gaps:73	98.89	449	29.50	20.72	2e-28	Lysine-sensitive aspartokinase 3 OS Escherichia coli (strain K12) GN lysC PE 1 SV 2
	sp\|P49079\|AKH1_MAIZE	30	523	494	Gaps:49	50.33	920	25.92	19.87	6e-28	Bifunctional aspartokinase/homoserine dehydrogenase 1 chloroplastic OS Zea mays GN AKHSDH1 PE 2 SV 1
	sp\|O81852\|AKH2_ARATH	30	523	494	Gaps:54	50.66	916	26.08	23.49	1e-27	Bifunctional aspartokinase/homoserine dehydrogenase 2 chloroplastic OS Arabidopsis thaliana GN AKHSDH2 PE 1 SV 1
blastp_pdb	3c20_B	32	519	488	Gaps:67	97.46	473	30.15	23.64	1e-40	mol:protein length:473 Probable aspartokinase
	3c20_A	32	519	488	Gaps:67	97.46	473	30.15	23.64	1e-40	mol:protein length:473 Probable aspartokinase
	3c1n_D	32	519	488	Gaps:67	97.46	473	30.15	23.64	1e-40	mol:protein length:473 Probable aspartokinase
	3c1n_C	32	519	488	Gaps:67	97.46	473	30.15	23.64	1e-40	mol:protein length:473 Probable aspartokinase
	3c1n_B	32	519	488	Gaps:67	97.46	473	30.15	23.64	1e-40	mol:protein length:473 Probable aspartokinase
	3c1n_A	32	519	488	Gaps:67	97.46	473	30.15	23.64	1e-40	mol:protein length:473 Probable aspartokinase
	3c1m_D	32	519	488	Gaps:67	97.46	473	30.15	23.64	1e-40	mol:protein length:473 Probable aspartokinase
	3c1m_C	32	519	488	Gaps:67	97.46	473	30.15	23.64	1e-40	mol:protein length:473 Probable aspartokinase
	3c1m_B	32	519	488	Gaps:67	97.46	473	30.15	23.64	1e-40	mol:protein length:473 Probable aspartokinase
	3c1m_A	32	519	488	Gaps:67	97.46	473	30.15	23.64	1e-40	mol:protein length:473 Probable aspartokinase
rpsblast_cdd	gnl\|CDD\|58613	30	350	321	Gaps:18	99.67	306	60.33	18.03	1e-120	cd04247 AAK_AK-Hom3 AAK_AK-Hom3: Amino Acid Kinase Superfamily (AAK) AK-Hom3 this CD includes the N-terminal catalytic domain of the aspartokinase HOM3 a monofunctional class enzyme found in Saccharomyces cerevisiae and other related AK domains. Aspartokinase the first enzyme in the aspartate metabolic pathway catalyzes the conversion of aspartate and ATP to aspartylphosphate and ADP and in fungi is responsible for the production of threonine isoleucine and methionine. S. cerevisiae has a single aspartokinase isoenzyme type which is regulated by feedback allosteric inhibition by L-threonine. Recent studies show that the allosteric transition triggered by binding of threonine to AK involves a large change in the conformation of the native hexameric enzyme that is converted to an inactive one of different shape and substantially smaller hydrodynamic size..
	gnl\|CDD\|30873	29	523	495	Gaps:65	99.33	447	34.01	18.24	1e-79	COG0527 LysC Aspartokinases [Amino acid transport and metabolism].
	gnl\|CDD\|161982	29	523	495	Gaps:93	99.77	441	35.23	20.23	7e-79	TIGR00657 asp_kinases aspartate kinase. The Lys-sensitive enzyme of Bacillus subtilis resembles the E. coli form but is an alpha 2/beta 2 heterotetramer where the beta subunit is translated from an in-phase alternative initiator at Met-246. This may be a feature of a number of closely related forms including a paralog from B. subtilis.
	gnl\|CDD\|169651	31	523	493	Gaps:82	99.33	448	33.03	20.90	1e-69	PRK09084 PRK09084 aspartate kinase III Validated.
	gnl\|CDD\|169870	30	523	494	Gaps:90	56.30	817	32.17	21.09	2e-67	PRK09436 thrA bifunctional aspartokinase I/homeserine dehydrogenase I Provisional.
	gnl\|CDD\|168511	31	519	489	Gaps:57	98.06	465	31.80	25.66	1e-65	PRK06291 PRK06291 aspartate kinase Provisional.
	gnl\|CDD\|166192	31	523	493	Gaps:68	88.69	513	35.38	19.34	3e-59	PLN02551 PLN02551 aspartate kinase.
	gnl\|CDD\|161981	161	523	363	Gaps:57	84.79	401	30.00	22.06	5e-44	TIGR00656 asp_kin_monofn aspartate kinase monofunctional class. The Lys-sensitive enzyme of Bacillus subtilis resembles the E. coli form but is an alpha 2/beta 2 heterotetramer where the beta subunit is translated from an in-phase alternative initiator at Met-246. The protein slr0657 from Synechocystis PCC6803 is extended by a duplication of the C-terminal region corresponding to the beta chain. Incorporation of a second copy of the C-terminal domain may be quite common in this subgroup of aspartokinases.
	gnl\|CDD\|58600	30	347	318	Gaps:11	99.56	227	40.27	18.58	3e-43	cd04234 AAK_AK AAK_AK: Amino Acid Kinase Superfamily (AAK) Aspartokinase (AK) this CD includes the N-terminal catalytic domain of aspartokinase (4-L-aspartate-4-phosphotransferase ). AK is the first enzyme in the biosynthetic pathway of the aspartate family of amino acids (lysine threonine methionine and isoleucine) and the bacterial cell wall component meso-diaminopimelate. It also catalyzes the conversion of aspartate and ATP to aspartylphosphate and ADP. One mechanism for the regulation of this pathway is by the production of several isoenzymes of aspartokinase with different repressors and allosteric inhibitors. Pairs of ACT domains are proposed to specifically bind amino acids leading to allosteric regulation of the enzyme. In Escherichia coli three different aspartokinase isoenzymes are regulated specifically by lysine methionine and threonine. AK-HSDHI (ThrA) and AK-HSDHII (MetL) are bifunctional enzymes that consist of an N-terminal AK and a C-terminal homoserine dehydrogenase (HSDH). ThrA and MetL are involved in threonine and methionine biosynthesis respectively. The third isoenzyme AKIII (LysC) is monofunctional and is involved in lysine synthesis. The three Bacillus subtilis isoenzymes AKI (DapG) AKII (LysC) and AKIII (YclM) are feedback-inhibited by meso-diaminopimelate lysine and lysine plus threonine respectively. The E. coli lysine-sensitive AK is described as a homodimer whereas the B. subtilis lysine-sensitive AK is described as a heterodimeric complex of alpha- and beta- subunits that are formed from two in-frame overlapping genes. A single AK enzyme type has been described in Pseudomonas Amycolatopsis and Corynebacterium. The fungal aspartate pathway is regulated at the AK step with L-Thr being an allosteric inhibitor of the Saccharomyces cerevisiae AK (Hom3). At least two distinct AK isoenzymes can occur in higher plants one is a monofunctional lysine-sensitive isoenzyme which is involved in the overall regulation of the pathway and can be synergistically inhibited by S-adenosylmethionine. The other isoenzyme is a bifunctional threonine-sensitive AK-HSDH protein. Also included in this CD is the catalytic domain of the Methylomicrobium alcaliphilum ectoine AK the first enzyme of the ectoine biosynthetic pathway found in this bacterium and several other halophilic/halotolerant bacteria..
	gnl\|CDD\|168633	163	520	358	Gaps:73	83.62	403	31.45	19.58	2e-42	PRK06635 PRK06635 aspartate kinase Reviewed.
rpsblast_kog	gnl\|CDD\|35677	24	523	500	Gaps:58	82.65	559	34.63	18.61	1e-118	KOG0456 KOG0456 KOG0456 Aspartate kinase [Amino acid transport and metabolism].

Other analysis

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Ortholog