Blast Analysis : gnl|CDD|197293

Analysis  rpsblast_cdd Start  238
End  416 Strand  +
Length  179 Note  Gaps:7
Hit Coverage  100.00 Hit Length  178
Hit Pident  51.69 E Val  9e-55
Hit Description  cd09197 PLDc_pPLDalpha_1 Catalytic domain repeat 1 of plant alpha-type phospholipase D. Catalytic domain repeat 1 of plant alpha-type phospholipase D (PLDalpha EC 3.1.4.4). Plant PLDalpha is a phosphatidylinositol 4 5-bisphosphate (PIP2)-independent PLD that possesses a regulatory calcium-dependent phospholipid-binding C2 domain in the N-terminus and require millimolar calcium for optimal activity. The C2 domain is unique to plant PLDs and is not present in animal or fungal PLDs. Like other PLD enzymes the monomer of plant PLDalpha consists of two catalytic domains each of which contains one copy of the conserved HKD motif (H-x-K-x(4)-D where x represents any amino acid residue). Two HKD motifs from two domains form a single active site. Plant PLDalpha may utilize a common two-step ping-pong catalytic mechanism involving an enzyme-substrate intermediate to cleave phosphodiester bonds. The two histidine residues from the two HKD motifs play key roles in the catalysis. Upon substrate binding a histidine residue from one HKD motif could function as the nucleophile attacking the phosphodiester bond to create a covalent phosphohistidine intermediate while the other histidine residue from the second HKD motif could serve as a general acid stabilizing the leaving group. Hit Pcons  15.73
Name  Qrob_P0238260.2
Paste the following link
Lists
This BlastAnalysis isn't in any lists. Upload a list.

1 Protein

Protein Identifier
Organism . Name 		Score Score Type Protein Description Alias (in v1) Code Enzyme Gene Prediction Quality
Qrob_P0238260.2 Quercus robur 100.0 egn (M=1) KOG1028//KOG1030//KOG1329//KOG2059 - Ca2+-dependent phospholipid-binding protein Synaptotagmin required for synaptic vesicle and secretory granule exocytosis [Signal transduction mechanisms Intracellular trafficking secretion and vesicular transport]. // Predicted Ca2+-dependent phospholipid-binding protein [General function prediction only]. // Phospholipase D1 [Lipid transport and metabolism]. // Ras GTPase-activating protein [Signal transduction mechanisms].   EC:3.1.4.4 validated