| Analysis | rpsblast_cdd | Start | 779 |
| End | 956 | Strand | + |
| Length | 178 | Note | Gaps:37 |
| Hit Coverage | 96.58 | Hit Length | 146 |
| Hit Pident | 34.04 | E Val | 2e-27 |
| Hit Description | cd09105 PLDc_vPLD1_2_like_2 Catalytic domain repeat 2 of vertebrate phospholipases PLD1 and PLD2 and similar proteins. Catalytic domain repeat 2 of phospholipase D (PLD EC 3.1.4.4) found in yeast plants and vertebrates and their bacterial homologs. PLDs are involved in signal transduction vesicle formation protein transport and mitosis by participating in phospholipid metabolism. They hydrolyze the terminal phosphodiester bond of phospholipids resulting in the formation of phosphatidic acid and alcohols. Phosphatidic acid is an essential compound involved in signal transduction. PLDs also catalyze the transphosphatidylation of phospholipids to acceptor alcohols by which various phospholipids can be synthesized. Both prokaryotic and eukaryotic PLDs have two HKD motifs (H-x-K-x(4)-D where x represents any amino acid residue) that characterizes the phospholipase D (PLD) superfamily. PLDs are active as bi-lobed monomers. Each monomer contains two domains each of which carries one copy of the HKD motif. Two HKD motifs from two domains form a single active site. PLDs utilize a common two-step ping-pong catalytic mechanism involving an enzyme-substrate intermediate to cleave phosphodiester bonds. The two histidine residues from the two HKD motifs play key roles in the catalysis. Upon substrate binding a histidine residue from one HKD motif could function as the nucleophile attacking the phosphodiester bond to create a covalent phosphohistidine intermediate while the other histidine residue from the second HKD motif could serve as a general acid stabilizing the leaving group. | Hit Pcons | 19.15 |
| Name | Qrob_P0154300.2 |
| Protein Identifier |
Organism . Name |
Score | Score Type | Protein Description | Alias (in v1) | Code Enzyme | Gene Prediction Quality |
|---|---|---|---|---|---|---|---|
| Qrob_P0154300.2 | Quercus robur | 96.0 | egn | (M=2) PTHR18896//PTHR18896:SF58 - PHOSPHOLIPASE D // SUBFAMILY NOT NAMED | EC:3.1.4.4 | validated |
Powered by
