| Analysis | rpsblast_cdd | Start | 772 |
| End | 958 | Strand | + |
| Length | 187 | Note | Gaps:5 |
| Hit Coverage | 100.00 | Hit Length | 182 |
| Hit Pident | 52.20 | E Val | 2e-55 |
| Hit Description | cd09845 PLDc_vPLD2_2 Catalytic domain repeat 2 of vertebrate phospholipase D2. Catalytic domain repeat 2 of vertebrate phospholipase D2 (PLD2). PLDs play a pivotal role in transmembrane signaling and cellular regulation. They hydrolyze the terminal phosphodiester bond of phospholipids with the formation of phosphatidic acid and alcohols. Phosphatidic acid is an essential compound involved in signal transduction. They also catalyze a transphosphatidylation of phospholipids to acceptor alcohols by which various phospholipids can be synthesized. Vertebrate PLD2 is a membrane associated phosphatidylinositol 4 5-bisphosphate (PIP2)-dependent enzyme that selectively hydrolyzes phosphatidylcholine (PC). Protein cofactors and calcium might be required for its activation. Most vertebrate PLDs have adjacent Phox (PX) and the Pleckstrin homology (PH) domains at their N-terminus which have been shown to mediate membrane targeting of the protein and are closely linked to polyphosphoinositide signaling. Like other members of the PLD superfamily the monomer of vertebrate PLDs consists of two catalytic domains each of which contains one copy of the conserved HKD motif (H-x-K-x(4)-D where x represents any amino acid residue). Two HKD motifs from two domains form a single active site. These PLDs utilize a common two-step ping-pong catalytic mechanism involving an enzyme-substrate intermediate to cleave phosphodiester bonds. The two histidine residues from the two HKD motifs play key roles in the catalysis. Upon substrate binding a histidine residue from one HKD motif could function as the nucleophile attacking the phosphodiester bond to create a covalent phosphohistidine intermediate while the other histidine residue from the second HKD motif could serve as a general acid stabilizing the leaving group. | Hit Pcons | 19.78 |
| Name | Qrob_P0154300.2 |
| Protein Identifier |
Organism . Name |
Score | Score Type | Protein Description | Alias (in v1) | Code Enzyme | Gene Prediction Quality |
|---|---|---|---|---|---|---|---|
| Qrob_P0154300.2 | Quercus robur | 96.0 | egn | (M=2) PTHR18896//PTHR18896:SF58 - PHOSPHOLIPASE D // SUBFAMILY NOT NAMED | EC:3.1.4.4 | validated |
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