| Analysis | rpsblast_cdd | Start | 53 |
| End | 93 | Strand | + |
| Length | 41 | Note | none |
| Hit Coverage | 29.29 | Hit Length | 140 |
| Hit Pident | 58.54 | E Val | 2e-09 |
| Hit Description | cd07968 OBF_DNA_ligase_IV The Oligonucleotide/oligosaccharide binding (OB)-fold domain of ATP-dependent DNA ligase IV is a DNA-binding module that is part of the catalytic core unit. ATP-dependent polynucleotide ligases catalyze phosphodiester bond formation using nicked nucleic acid substrates with the high energy nucleotide of ATP as a cofactor in a three step reaction mechanism. DNA ligases play a vital role in the diverse processes of DNA replication recombination and repair. ATP-dependent ligases are present in many organisms such as viruses bacteriohages eukarya archaea and bacteria. There are three classes of ATP-dependent DNA ligases in eukaryotic cells (I III and IV). DNA ligase IV is required for DNA non-homologous end joining pathways including recombination of the V(D)J immunoglobulin gene segments in cells of the mammalian immune system. DNA ligase IV is stabilized by forming a complex with XRCC4 a nuclear phosphoprotein which is phosphorylated by DNA-dependent protein kinase. DNA ligases have a highly modular architecture consisting of a unique arrangement of two or more discrete domains. The adenylation and C-terminal oligouncleotide/oligosaccharide binding (OB)-fold domains comprise a catalytic core unit that is common to most members of the ATP-dependent DNA ligase family. The catalytic core unit contains six conserved sequence motifs (I III IIIa IV V and VI) that define this family of related nucleotidyltransferases. The OB-fold domain contacts the nicked DNA substrate and is required for the ATP-dependent DNA ligase nucleotidylation step. The RxDK motif (motif VI) which is essential for ATP hydrolysis is located in the OB-fold domain. | Hit Pcons | 12.20 |
| Name | Qrob_P0078600.2 |
| Protein Identifier |
Organism . Name |
Score | Score Type | Protein Description | Alias (in v1) | Code Enzyme | Gene Prediction Quality |
|---|---|---|---|---|---|---|---|
| Qrob_P0078600.2 | Quercus robur | 99.1 | egn | (M=2) 6.5.1.1 - DNA ligase (ATP). | EC:6.5.1.1 | validated |
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