Protein : Qrob_P0759650.2 Q. robur

Protein Identifier  ? Qrob_P0759650.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=2) 4.2.1.65 - 3-cyanoalanine hydratase. Code Enzyme  EC:3.5.5.4, EC:4.2.1.65
Gene Prediction Quality  validated Protein length 

Sequence

Length: 160  

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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0006807 nitrogen compound metabolic process The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen, including (but not limited to) nitrogen fixation, nitrification, denitrification, assimilatory/dissimilatory nitrate reduction and the interconversion of nitrogenous organic matter and ammonium.
GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Catalysis of the hydrolysis of any carbon-nitrogen bond, C-N, with the exception of peptide bonds.

21 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|tcc:TCM_022798 2 157 + 156 Gaps:75 52.16 439 41.92 4e-40 Nitrilase 4 isoform 1
blastp_kegg lcl|pvu:PHAVU_011G096700g 2 133 + 132 Gaps:22 42.86 350 44.00 4e-31 hypothetical protein
blastp_kegg lcl|gmx:100810230 2 133 + 132 Gaps:22 42.86 350 43.33 2e-30 bifunctional nitrilase/nitrile hydratase NIT4B-like
blastp_kegg lcl|sot:102595618 2 133 + 132 Gaps:22 42.74 351 44.00 2e-30 bifunctional nitrilase/nitrile hydratase NIT4B-like
blastp_kegg lcl|gmx:100778110 2 133 + 132 Gaps:22 42.86 350 42.00 2e-30 bifunctional nitrilase/nitrile hydratase NIT4A-like
blastp_kegg lcl|sly:101266793 2 133 + 132 Gaps:22 42.74 351 43.33 3e-30 bifunctional nitrilase/nitrile hydratase NIT4B-like
blastp_kegg lcl|cic:CICLE_v10028755mg 2 133 + 132 Gaps:22 43.48 345 42.67 5e-30 hypothetical protein
blastp_kegg lcl|cit:102626612 2 133 + 132 Gaps:22 43.48 345 42.67 5e-30 bifunctional nitrilase/nitrile hydratase NIT4A-like
blastp_kegg lcl|pper:PRUPE_ppa008090mg 2 133 + 132 Gaps:22 43.48 345 42.67 2e-29 hypothetical protein
blastp_kegg lcl|rcu:RCOM_1282290 2 133 + 132 Gaps:22 46.15 325 44.00 2e-29 Nitrilase putative (EC:3.5.5.1)
blastp_uniprot_sprot sp|Q42965|NRL4A_TOBAC 2 133 + 132 Gaps:22 42.98 349 40.67 7e-30 Bifunctional nitrilase/nitrile hydratase NIT4A OS Nicotiana tabacum GN NIT4A PE 2 SV 1
blastp_uniprot_sprot sp|P46011|NRL4_ARATH 1 133 + 133 Gaps:21 42.82 355 41.45 3e-29 Bifunctional nitrilase/nitrile hydratase NIT4 OS Arabidopsis thaliana GN NIT4 PE 1 SV 1
blastp_uniprot_sprot sp|Q42966|NRL4B_TOBAC 2 109 + 108 Gaps:21 36.49 348 44.88 4e-29 Bifunctional nitrilase/nitrile hydratase NIT4B OS Nicotiana tabacum GN NIT4B PE 2 SV 1
blastp_uniprot_sprot sp|Q6H849|NRL4_ORYSJ 2 133 + 132 Gaps:22 41.44 362 41.33 6e-29 Bifunctional nitrilase/nitrile hydratase NIT4 OS Oryza sativa subsp. japonica GN NIT4 PE 2 SV 1
blastp_uniprot_sprot sp|P46010|NRL3_ARATH 7 109 + 103 Gaps:20 35.55 346 43.09 2e-23 Nitrilase 3 OS Arabidopsis thaliana GN NIT3 PE 2 SV 1
blastp_uniprot_sprot sp|P32961|NRL1_ARATH 7 109 + 103 Gaps:20 35.55 346 37.40 5e-21 Nitrilase 1 OS Arabidopsis thaliana GN NIT1 PE 1 SV 2
blastp_uniprot_sprot sp|P32962|NRL2_ARATH 7 107 + 101 Gaps:20 35.69 339 38.02 5e-20 Nitrilase 2 OS Arabidopsis thaliana GN NIT2 PE 2 SV 1
blastp_uniprot_sprot sp|Q89PT3|NITR2_BRADU 12 110 + 99 Gaps:20 37.07 321 36.13 9e-14 Nitrilase blr3397 OS Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) GN blr3397 PE 1 SV 1
rpsblast_cdd gnl|CDD|178120 2 133 + 132 Gaps:22 43.35 346 42.67 2e-35 PLN02504 PLN02504 nitrilase.
rpsblast_cdd gnl|CDD|143588 12 109 + 98 Gaps:26 41.75 297 30.65 1e-15 cd07564 nitrilases_CHs Nitrilases cyanide hydratase (CH)s and similar proteins (class 1 nitrilases). Nitrilases (nitrile aminohydrolases EC:3.5.5.1) hydrolyze nitriles (RCN) to ammonia and the corresponding carboxylic acid. Most nitrilases prefer aromatic nitriles some prefer arylacetonitriles and others aliphatic nitriles. This group includes the nitrilase cyanide dihydratase (CDH) which hydrolyzes inorganic cyanide (HCN) to produce formate. It also includes cyanide hydratase (CH) which hydrolyzes HCN to formamide. This group includes four Arabidopsis thaliana nitrilases (Ath)NIT1-4. AthNIT1-3 have a strong substrate preference for phenylpropionitrile (PPN) and other nitriles which may originate from the breakdown of glucosinolates. The product of PPN hydrolysis phenylacetic acid has auxin activity. AthNIT1-3 can also convert indoacetonitrile to indole-3-acetic acid (IAA auxin) but with a lower affinity and velocity. From their expression patterns it has been speculated that NIT3 may produce IAA during the early stages of germination and that NIT3 may produce IAA during embryo development and maturation. AthNIT4 has a strong substrate specificity for the nitrile beta-cyano-L-alanine (Ala(CN)) an intermediate of cyanide detoxification. AthNIT4 has both a nitrilase activity and a nitrile hydratase (NHase) activity which generate aspartic acid and asparagine respectively from Ala(CN). NHase catalyzes the hydration of nitriles to their corresponding amides. This subgroup belongs to a larger nitrilase superfamily comprised of belong to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) this subgroup corresponds to class 1.
rpsblast_kog gnl|CDD|36023 7 109 + 103 Gaps:20 36.50 337 41.46 8e-26 KOG0805 KOG0805 KOG0805 Carbon-nitrogen hydrolase [Amino acid transport and metabolism].

8 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
SUPERFAMILY 13 110 98 SSF56317 none none IPR003010
Gene3D 12 71 60 G3DSA:3.60.110.10 none none IPR003010
Phobius 17 36 20 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Gene3D 72 111 40 G3DSA:3.60.110.10 none none IPR003010
Phobius 1 16 16 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 37 159 123 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
PANTHER 12 109 98 PTHR23088 none none none
PANTHER 12 109 98 PTHR23088:SF13 none none none

1 Localization

Analysis Start End Length
TMHMM 13 35 22

0 Qtllist

0 Targeting