Protein : Qrob_P0721900.2 Q. robur
Protein Identifier  ? Qrob_P0721900.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=4) 5.4.99.24 - 23S rRNA pseudouridine(955/2504/2580) synthase. Code Enzyme  EC:5.4.99.24
Gene Prediction Quality  validated Protein length 

Sequence

Length: 473  
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0003723 RNA binding Interacting selectively and non-covalently with an RNA molecule or a portion thereof.
GO:0001522 pseudouridine synthesis The intramolecular conversion of uridine to pseudouridine within an RNA molecule. This posttranscriptional base modification occurs in tRNA, rRNA, and snRNAs.
GO:0009451 RNA modification The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically.
GO:0009982 pseudouridine synthase activity Catalysis of the reaction: RNA uridine = RNA pseudouridine. Conversion of uridine in an RNA molecule to pseudouridine by rotation of the C1'-N-1 glycosidic bond of uridine in RNA to a C1'-C5.

39 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pvu:PHAVU_001G032500g 1 472 + 472 Gaps:8 100.00 480 75.62 0.0 hypothetical protein
blastp_kegg lcl|vvi:100259844 1 472 + 472 Gaps:7 100.00 479 79.54 0.0 RNA pseudourine synthase 3 mitochondrial-like
blastp_kegg lcl|cam:101507491 8 472 + 465 Gaps:4 97.91 479 77.19 0.0 RNA pseudourine synthase 3 mitochondrial-like
blastp_kegg lcl|pper:PRUPE_ppa005240mg 1 472 + 472 Gaps:9 100.00 471 77.71 0.0 hypothetical protein
blastp_kegg lcl|pmum:103337742 1 472 + 472 Gaps:15 100.00 471 78.34 0.0 RNA pseudouridine synthase 3 mitochondrial
blastp_kegg lcl|csv:101230792 9 472 + 464 Gaps:7 98.73 471 76.77 0.0 RNA pseudourine synthase 3 mitochondrial-like
blastp_kegg lcl|tcc:TCM_027759 10 472 + 463 Gaps:8 98.30 471 80.35 0.0 Pseudouridine synthase family protein
blastp_kegg lcl|csv:101219471 9 472 + 464 Gaps:7 98.73 471 76.56 0.0 RNA pseudourine synthase 3 mitochondrial-like
blastp_kegg lcl|gmx:100817786 7 472 + 466 Gaps:7 98.11 476 76.45 0.0 RNA pseudouridine synthase 3 mitochondrial-like
blastp_kegg lcl|cmo:103485411 9 472 + 464 Gaps:7 98.73 473 76.87 0.0 RNA pseudouridine synthase 3 mitochondrial
blastp_pdb 2i82_D 180 368 + 189 Gaps:28 75.12 217 37.42 1e-11 mol:protein length:217 Ribosomal large subunit pseudouridine synthas
blastp_pdb 2i82_C 180 368 + 189 Gaps:28 75.12 217 37.42 1e-11 mol:protein length:217 Ribosomal large subunit pseudouridine synthas
blastp_pdb 2i82_B 180 368 + 189 Gaps:28 75.12 217 37.42 1e-11 mol:protein length:217 Ribosomal large subunit pseudouridine synthas
blastp_pdb 2i82_A 180 368 + 189 Gaps:28 75.12 217 37.42 1e-11 mol:protein length:217 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1v9k_B 170 368 + 199 Gaps:48 75.00 228 36.84 6e-11 mol:protein length:228 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1v9k_A 170 368 + 199 Gaps:48 75.00 228 36.84 6e-11 mol:protein length:228 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1xpi_B 180 368 + 189 Gaps:45 71.00 231 35.98 6e-11 mol:protein length:231 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1xpi_A 180 368 + 189 Gaps:45 71.00 231 35.98 6e-11 mol:protein length:231 Ribosomal large subunit pseudouridine synthas
blastp_uniprot_sprot sp|Q5XET6|PUS3_ARATH 1 472 + 472 Gaps:14 100.00 478 72.18 0.0 RNA pseudouridine synthase 3 mitochondrial OS Arabidopsis thaliana GN At1g78910 PE 2 SV 1
blastp_uniprot_sprot sp|Q0J4D4|PUS3_ORYSJ 32 472 + 441 Gaps:5 93.89 475 65.47 0.0 RNA pseudouridine synthase 3 mitochondrial OS Oryza sativa subsp. japonica GN Os08g0520100 PE 2 SV 1
blastp_uniprot_sprot sp|Q9LT72|PUS4_ARATH 55 469 + 415 Gaps:27 86.79 477 38.41 3e-77 RNA pseudouridine synthase 4 mitochondrial OS Arabidopsis thaliana GN At3g19440 PE 2 SV 1
blastp_uniprot_sprot sp|Q69K07|PUS4_ORYSJ 80 348 + 269 Gaps:23 58.48 448 38.17 5e-46 RNA pseudouridine synthase 4 mitochondrial OS Oryza sativa subsp. japonica GN Os09g0103500 PE 2 SV 1
blastp_uniprot_sprot sp|Q28C59|RUSD4_XENTR 169 424 + 256 Gaps:64 76.54 324 36.69 7e-28 RNA pseudouridylate synthase domain-containing protein 4 OS Xenopus tropicalis GN rpusd4 PE 2 SV 1
blastp_uniprot_sprot sp|O66114|Y505_ZYMMO 179 389 + 211 Gaps:40 89.95 219 35.53 3e-21 Uncharacterized RNA pseudouridine synthase ZMO0505 OS Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) GN ZMO0505 PE 3 SV 2
blastp_uniprot_sprot sp|Q6DBR0|RUSD4_DANRE 180 425 + 246 Gaps:61 65.08 358 35.19 5e-21 RNA pseudouridylate synthase domain-containing protein 4 OS Danio rerio GN rpusd4 PE 2 SV 1
blastp_uniprot_sprot sp|Q4UKQ3|RLUC_RICFE 150 368 + 219 Gaps:41 59.67 305 39.56 2e-19 Ribosomal large subunit pseudouridine synthase C OS Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) GN rluC PE 3 SV 1
blastp_uniprot_sprot sp|Q68XB2|RLUC_RICTY 174 368 + 195 Gaps:30 56.44 303 38.60 2e-19 Ribosomal large subunit pseudouridine synthase C OS Rickettsia typhi (strain ATCC VR-144 / Wilmington) GN rluC PE 3 SV 1
blastp_uniprot_sprot sp|Q92IS6|RLUC_RICCN 150 368 + 219 Gaps:41 60.07 303 39.01 3e-19 Ribosomal large subunit pseudouridine synthase C OS Rickettsia conorii (strain ATCC VR-613 / Malish 7) GN rluC PE 3 SV 1
rpsblast_cdd gnl|CDD|211346 183 379 + 197 Gaps:26 94.59 185 37.71 4e-37 cd02869 PseudoU_synth_RluCD_like Pseudouridine synthase RsuA/RluD family. This group is comprised of eukaryotic bacterial and archeal proteins similar to eight site specific Escherichia coli pseudouridine synthases: RsuA RluA RluB RluC RluD RluE RluF and TruA. Pseudouridine synthases catalyze the isomerization of specific uridines in a n RNA molecule to pseudouridines (5-ribosyluracil psi) requiring no cofactors. E. coli RluC for example makes psi955 2504 and 2580 in 23S RNA. Some psi sites such as psi1917 in 23S RNA made by RluD are universally conserved. Other psi sites occur in a more restricted fashion for example psi2819 in 21S mitochondrial ribosomal RNA made by S. cerevisiae Pus5p is only found in mitochondrial large subunit rRNAs from some other species and in gram negative bacteria. The E. coli counterpart of this psi residue is psi2580 in 23S rRNA. psi2604in 23S RNA made by RluF has only been detected in E.coli.
rpsblast_cdd gnl|CDD|30910 129 368 + 240 Gaps:37 71.63 289 36.23 5e-33 COG0564 RluA Pseudouridylate synthases 23S RNA-specific [Translation ribosomal structure and biogenesis].

7 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 79 426 348 PTHR10436:SF94 none none none
PANTHER 444 472 29 PTHR10436:SF94 none none none
Pfam 183 354 172 PF00849 none RNA pseudouridylate synthase IPR006145
SUPERFAMILY 413 425 13 SSF55120 none none IPR020103
SUPERFAMILY 180 385 206 SSF55120 none none IPR020103
PANTHER 79 426 348 PTHR10436 none none none
PANTHER 444 472 29 PTHR10436 none none none

0 Localization

17 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran1_2003_QTL2_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 87 lod 3,3 8,7
Bourran2_2004_QTL9_peak_Bud_burst_3P Qrob_Chr02 2 s_1C34E9_788 v_12238_322 50 25 75 lod 4,4 10,1
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL2_d13Cf Qrob_Chr02 2 s_1AQA4Z_1644 s_1AK5QX_947 53.67 14,01 79,68 lod 5.6594 0.03
Bourran1_2004_QTL2_peak_Bud_burst_3P Qrob_Chr02 2 s_1AW12F_382 s_1A77MR_223 42 6 64 lod 3,6 9,6
Bourran2_2002_QTL7_peak_Bud_burst_3P Qrob_Chr02 2 s_1ANG6_1446 v_11270_161 40 29 52 lod 8,1 16
Bourran2_2002_QTL9_peak_Bud_burst_A4 Qrob_Chr02 2 s_1BFNDA_375 s_1A3VA1_2139 32,5 17 62 lod 3,1 4,2
Bourran2_2003_QTL8_peak_Bud_burst_3P Qrob_Chr02 2 s_1ANG6_1446 v_11270_161 40 0 72 lod 4,4 9,9
Bourran2_2014_nP_A4 Qrob_Chr11 11 s_1B58GB_1413 s_1A5BYY_1671 11,15 0 42,38 lod 1,8913 4,5
Bourran2_2015_nP_A4 Qrob_Chr02 2 s_1A0FUE_1868 s_1A1UAI_500 20,64 20,47 21,36 lod 5.8 10.9
Bourran2_2015_nPriLBD_A4 Qrob_Chr02 2 s_1CP5DI_1183 s_1A63ZX_1277 24,87 24,63 26,18 lod 3.8 7
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL6_d13Cf Qrob_Chr02 2 s_1AEP21_172 v_6048_204 46.33 22,5 65,23 lod 4.972 0.03
Bourran2_2015_nEpis_A4 Qrob_Chr09 9 v_15847_485 v_8329_369 34,94 34,88 37,45 lod 3.1 7
Bourran2_2015_nSecLBD_A4 Qrob_Chr09 9 v_15847_485 v_8329_369 35,81 34,88 37,45 lod 4.4 10.4
Bourran1_2004_QTL3_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 46 lod 2,9 6,4
Bourran2_2015_nEpiBC_A4 Qrob_Chr07 7 s_1DP9TW_798 v_8128_173 22,61 22,14 22,73 lod 3.1 8.5
Champenoux_2015_nP_A4 Qrob_Chr02 2 s_1BN4CB_644 v_508_128 23,76 23,06 24,51 lod 2.8 6.2
Champenoux_2015_nPriLBD_A4 Qrob_Chr02 2 s_1CP5DI_1183 s_1A63ZX_1277 25,35 24,63 26,18 lod 4.0 8.7

0 Targeting