Protein : Qrob_P0619140.2 Q. robur
Protein Identifier  ? Qrob_P0619140.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=3) 3.5.5.1//3.5.5.4 - Nitrilase. // Cyanoalanine nitrilase. Code Enzyme  EC:3.5.5.4, EC:3.5.5.1
Gene Prediction Quality  validated Protein length 

Sequence

Length: 148  
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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0006807 nitrogen compound metabolic process The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen, including (but not limited to) nitrogen fixation, nitrification, denitrification, assimilatory/dissimilatory nitrate reduction and the interconversion of nitrogenous organic matter and ammonium.
GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Catalysis of the hydrolysis of any carbon-nitrogen bond, C-N, with the exception of peptide bonds.

28 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|cic:CICLE_v10028755mg 1 106 + 106 Gaps:2 30.14 345 90.38 1e-54 hypothetical protein
blastp_kegg lcl|fve:101314432 1 106 + 106 Gaps:1 28.85 364 85.71 1e-53 bifunctional nitrilase/nitrile hydratase NIT4B-like
blastp_kegg lcl|mdm:103424813 1 106 + 106 Gaps:2 30.06 346 89.42 1e-52 bifunctional nitrilase/nitrile hydratase NIT4B
blastp_kegg lcl|vvi:100250098 1 106 + 106 Gaps:3 30.84 347 85.05 1e-52 bifunctional nitrilase/nitrile hydratase NIT4B-like
blastp_kegg lcl|cit:102626612 1 106 + 106 Gaps:2 30.14 345 87.50 2e-52 bifunctional nitrilase/nitrile hydratase NIT4A-like
blastp_kegg lcl|pper:PRUPE_ppa008090mg 1 106 + 106 Gaps:3 29.86 345 88.35 1e-50 hypothetical protein
blastp_kegg lcl|tcc:TCM_041340 1 106 + 106 Gaps:6 20.45 538 83.64 1e-50 Bifunctional nitrilase/nitrile hydratase NIT4 isoform 1
blastp_kegg lcl|sly:101266793 1 106 + 106 Gaps:4 30.77 351 84.26 3e-50 bifunctional nitrilase/nitrile hydratase NIT4B-like
blastp_kegg lcl|cit:102624718 1 105 + 105 Gaps:1 29.38 354 80.77 5e-50 bifunctional nitrilase/nitrile hydratase NIT4A-like
blastp_kegg lcl|pmum:103329255 1 106 + 106 Gaps:3 29.86 345 88.35 7e-50 bifunctional nitrilase/nitrile hydratase NIT4A
blastp_uniprot_sprot sp|Q42966|NRL4B_TOBAC 1 106 + 106 Gaps:4 31.03 348 85.19 1e-53 Bifunctional nitrilase/nitrile hydratase NIT4B OS Nicotiana tabacum GN NIT4B PE 2 SV 1
blastp_uniprot_sprot sp|Q42965|NRL4A_TOBAC 1 106 + 106 Gaps:4 30.95 349 84.26 3e-52 Bifunctional nitrilase/nitrile hydratase NIT4A OS Nicotiana tabacum GN NIT4A PE 2 SV 1
blastp_uniprot_sprot sp|Q6H849|NRL4_ORYSJ 1 106 + 106 Gaps:10 31.49 362 73.68 2e-43 Bifunctional nitrilase/nitrile hydratase NIT4 OS Oryza sativa subsp. japonica GN NIT4 PE 2 SV 1
blastp_uniprot_sprot sp|P46011|NRL4_ARATH 1 106 + 106 Gaps:2 29.86 355 69.81 5e-42 Bifunctional nitrilase/nitrile hydratase NIT4 OS Arabidopsis thaliana GN NIT4 PE 1 SV 1
blastp_uniprot_sprot sp|P46010|NRL3_ARATH 17 106 + 90 Gaps:2 25.43 346 64.77 4e-31 Nitrilase 3 OS Arabidopsis thaliana GN NIT3 PE 2 SV 1
blastp_uniprot_sprot sp|P32961|NRL1_ARATH 25 106 + 82 none 23.70 346 64.63 4e-30 Nitrilase 1 OS Arabidopsis thaliana GN NIT1 PE 1 SV 2
blastp_uniprot_sprot sp|P32962|NRL2_ARATH 18 106 + 89 Gaps:2 25.66 339 65.52 4e-30 Nitrilase 2 OS Arabidopsis thaliana GN NIT2 PE 2 SV 1
blastp_uniprot_sprot sp|Q89PT3|NITR2_BRADU 19 106 + 88 none 27.41 321 46.59 2e-18 Nitrilase blr3397 OS Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) GN blr3397 PE 1 SV 1
blastp_uniprot_sprot sp|P40447|NIT1_YEAST 29 145 + 117 Gaps:14 51.76 199 40.78 1e-13 Putative nitrilase-like protein NIT1 OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN NIT1 PE 5 SV 1
blastp_uniprot_sprot sp|P82605|NRL_BACSX 28 105 + 78 Gaps:5 21.53 339 42.47 3e-09 Nitrilase OS Bacillus sp. (strain OxB-1) GN nit PE 1 SV 2
rpsblast_cdd gnl|CDD|178120 10 106 + 97 Gaps:1 27.75 346 79.17 4e-48 PLN02504 PLN02504 nitrilase.
rpsblast_cdd gnl|CDD|143588 27 106 + 80 Gaps:5 25.25 297 53.33 5e-26 cd07564 nitrilases_CHs Nitrilases cyanide hydratase (CH)s and similar proteins (class 1 nitrilases). Nitrilases (nitrile aminohydrolases EC:3.5.5.1) hydrolyze nitriles (RCN) to ammonia and the corresponding carboxylic acid. Most nitrilases prefer aromatic nitriles some prefer arylacetonitriles and others aliphatic nitriles. This group includes the nitrilase cyanide dihydratase (CDH) which hydrolyzes inorganic cyanide (HCN) to produce formate. It also includes cyanide hydratase (CH) which hydrolyzes HCN to formamide. This group includes four Arabidopsis thaliana nitrilases (Ath)NIT1-4. AthNIT1-3 have a strong substrate preference for phenylpropionitrile (PPN) and other nitriles which may originate from the breakdown of glucosinolates. The product of PPN hydrolysis phenylacetic acid has auxin activity. AthNIT1-3 can also convert indoacetonitrile to indole-3-acetic acid (IAA auxin) but with a lower affinity and velocity. From their expression patterns it has been speculated that NIT3 may produce IAA during the early stages of germination and that NIT3 may produce IAA during embryo development and maturation. AthNIT4 has a strong substrate specificity for the nitrile beta-cyano-L-alanine (Ala(CN)) an intermediate of cyanide detoxification. AthNIT4 has both a nitrilase activity and a nitrile hydratase (NHase) activity which generate aspartic acid and asparagine respectively from Ala(CN). NHase catalyzes the hydration of nitriles to their corresponding amides. This subgroup belongs to a larger nitrilase superfamily comprised of belong to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) this subgroup corresponds to class 1.
rpsblast_cdd gnl|CDD|201448 28 114 + 87 Gaps:17 40.70 172 38.57 2e-12 pfam00795 CN_hydrolase Carbon-nitrogen hydrolase. This family contains hydrolases that break carbon-nitrogen bonds. The family includes: Nitrilase EC:3.5.5.1 Aliphatic amidase EC:3.5.1.4 Biotidinase EC:3.5.1.12 Beta-ureidopropionase EC:3.5.1.6. Nitrilase-related proteins generally have a conserved E-K-C catalytic triad and are multimeric alpha-beta-beta-alpha sandwich proteins.
rpsblast_cdd gnl|CDD|143587 29 73 + 45 none 17.79 253 46.67 6e-09 cd07197 nitrilase Nitrilase superfamily including nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes. This superfamily (also known as the C-N hydrolase superfamily) contains hydrolases that break carbon-nitrogen bonds it includes nitrilases cyanide dihydratases aliphatic amidases N-terminal amidases beta-ureidopropionases biotinidases pantotheinase N-carbamyl-D-amino acid amidohydrolases the glutaminase domain of glutamine-dependent NAD+ synthetase apolipoprotein N-acyltransferases and N-carbamoylputrescine amidohydrolases among others. These enzymes depend on a Glu-Lys-Cys catalytic triad and work through a thiol acylenzyme intermediate. Members of this superfamily generally form homomeric complexes the basic building block of which is a homodimer. These oligomers include dimers tetramers hexamers octamers tetradecamers octadecamers as well as variable length helical arrangements and homo-oligomeric spirals. These proteins have roles in vitamin and co-enzyme metabolism in detoxifying small molecules in the synthesis of signaling molecules and in the post-translational modification of proteins. They are used industrially as biocatalysts in the fine chemical and pharmaceutical industry in cyanide remediation and in the treatment of toxic effluent. This superfamily has been classified previously in the literature based on global and structure-based sequence analysis into thirteen different enzyme classes (referred to as 1-13). This hierarchy includes those thirteen classes and a few additional subfamilies. A putative distant relative the plasmid-borne TraB family has not been included in the hierarchy.
rpsblast_cdd gnl|CDD|188563 25 106 + 82 Gaps:5 25.58 301 45.45 2e-08 TIGR04048 nitrile_sll0784 putative nitrilase sll0784 family. This family represents a subfamily of a C-N bond-cleaving hydrolases (see pfam00795). Members occur as part of a cluster of genes in a probable biosynthetic cluster that contains a radical SAM protein an N-acetyltransferase a flavoprotein several proteins of unknown function and usually a glycosyltransferase. Members are closely related to a characterized aliphatic nitrilase from Rhodopseudomonas rhodochrous J1 for which an active site Cys was found at position 165.
rpsblast_cdd gnl|CDD|30737 27 74 + 48 none 17.52 274 45.83 2e-08 COG0388 COG0388 Predicted amidohydrolase [General function prediction only].
rpsblast_cdd gnl|CDD|143600 28 78 + 51 none 20.08 254 37.25 1e-07 cd07576 R-amidase_like Pseudomonas sp. MCI3434 R-amidase and related proteins (putative class 13 nitrilases). Pseudomonas sp. MCI3434 R-amidase hydrolyzes (R S)-piperazine-2-tert-butylcarboxamide to form (R)-piperazine-2-carboxylic acid. It does so with strict R-stereoselectively. Its preferred substrates are carboxamide compounds which have the amino or imino group connected to their beta- or gamma-carbon. This subgroup belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13) class 13 represents proteins that at the time were difficult to place in a distinct similarity group. It has been suggested that this subgroup represents a new class. Members of the nitrilase superfamily generally form homomeric complexes the basic building block of which is a homodimer. Native R-amidase however appears to be a monomer.
rpsblast_kog gnl|CDD|36023 18 106 + 89 Gaps:2 25.82 337 70.11 4e-34 KOG0805 KOG0805 KOG0805 Carbon-nitrogen hydrolase [Amino acid transport and metabolism].

7 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
ProSiteProfiles 27 147 121 PS50263 none Carbon-nitrogen hydrolase domain profile. IPR003010
SUPERFAMILY 25 113 89 SSF56317 none none IPR003010
ProSitePatterns 59 74 16 PS00920 none Nitrilases / cyanide hydratase signature 1. IPR000132
Pfam 28 113 86 PF00795 none Carbon-nitrogen hydrolase IPR003010
PANTHER 22 103 82 PTHR23088:SF13 none none none
PANTHER 22 103 82 PTHR23088 none none none
Gene3D 26 109 84 G3DSA:3.60.110.10 none none IPR003010

0 Localization

10 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran1_2004_QTL1_peak_Bud_burst_A4 Qrob_Chr01 1 s_1AMVCC_444 s_1BE1VC_321 4 0 31 lod 3,2 7,4
Bourran2_2003_QTL8_peak_Bud_burst_A4 Qrob_Chr01 1 s_1AH7I_610 s_1BE1VC_321 4,6 0 28 lod 3,5 7,4
Bourran2_2007_QTL6_peak_Bud_burst_3P Qrob_Chr01 1 s_1BE1VC_321 s_1A5JRZ_890 5 0 25 lod 2,4 6
Bourran2_2014_nEpiBC_3P Qrob_Chr01 1 v_1588_550 s_1AZJRA_1600 30,48 9,48 43,98 lod 2,3642 6,3
Bourran2_2014_nLBD*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 34,91 16,12 53,62 lod 2,4961 5,2
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2014_rEpiBC*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 35,77 14,11 55,31 lod 2,9413 6,2
Bourran2_2015_nEpiBC_3P Qrob_Chr12 12 s_1B73S5_217 v_7050_211 28,31 26,37 28,45 lod 4.5 11.6
Bourran2_2015_rEpiBC_3P Qrob_Chr08 8 s_A9TNV_543 v_11837_70 9,93 9,83 11,15 lod 3.3 7.3
Bourran2_2014_nSeqBC*_A4 Qrob_Chr01 1 s_1CFE4C_2114 s_1BDNCB_819 15,25 2 28,5 lod 2,9903 8,3

0 Targeting