Protein : Qrob_P0577550.2 Q. robur
Protein Identifier  ? Qrob_P0577550.2 Organism . Name  Quercus robur
Score  65.0 Score Type  egn
Protein Description  (M=5) 2.3.3.13 - 2-isopropylmalate synthase. Code Enzyme  EC:2.3.3.13
Gene Prediction Quality  validated Protein length 

Sequence

Length: 169  
Kegg Orthology  K01649
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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0003852 2-isopropylmalate synthase activity Catalysis of the reaction: 3-methyl-2-oxobutanoate + acetyl-CoA + H(2)O = (2S)-2-isopropylmalate + CoA + H(+).
GO:0009098 leucine biosynthetic process The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

28 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|mdm:103455378 1 167 + 167 none 83.50 200 80.84 2e-91 2-isopropylmalate synthase 1 chloroplastic-like
blastp_kegg lcl|pper:PRUPE_ppa004868mg 1 167 + 167 none 34.22 488 83.23 2e-90 hypothetical protein
blastp_kegg lcl|pxb:103942671 3 167 + 165 none 39.10 422 80.61 1e-87 2-isopropylmalate synthase 1 chloroplastic-like
blastp_kegg lcl|pmum:103331792 3 167 + 165 Gaps:1 26.22 633 83.13 1e-87 2-isopropylmalate synthase 1 chloroplastic-like
blastp_kegg lcl|mdm:103432269 1 167 + 167 none 98.24 170 76.65 1e-85 2-isopropylmalate synthase 1 chloroplastic-like
blastp_kegg lcl|mdm:103426151 5 167 + 163 none 59.93 272 77.91 2e-83 2-isopropylmalate synthase 1 chloroplastic-like
blastp_kegg lcl|cic:CICLE_v10020218mg 3 167 + 165 none 38.11 433 79.39 5e-83 hypothetical protein
blastp_kegg lcl|pop:POPTR_0005s08480g 3 158 + 156 none 31.90 489 80.77 8e-82 POPTRDRAFT_861364 hypothetical protein
blastp_kegg lcl|cit:102620455 3 167 + 165 none 26.61 620 79.39 1e-80 2-isopropylmalate synthase 1 chloroplastic-like
blastp_kegg lcl|csv:101205698 3 152 + 150 none 23.70 633 84.00 7e-80 2-isopropylmalate synthase 2 chloroplastic-like
blastp_uniprot_sprot sp|Q9LPR4|LEU11_ARATH 3 167 + 165 none 26.15 631 75.76 4e-81 2-isopropylmalate synthase 1 chloroplastic OS Arabidopsis thaliana GN IPMS1 PE 1 SV 2
blastp_uniprot_sprot sp|Q9C550|LEU12_ARATH 3 166 + 164 Gaps:2 26.31 631 76.51 2e-80 2-isopropylmalate synthase 2 chloroplastic OS Arabidopsis thaliana GN IPMS2 PE 1 SV 1
blastp_uniprot_sprot sp|O04974|LEU1B_SOLPN 3 166 + 164 none 26.80 612 70.12 8e-76 2-isopropylmalate synthase B OS Solanum pennellii GN IPMSB PE 2 SV 1
blastp_uniprot_sprot sp|O04973|LEU1A_SOLPN 3 153 + 151 none 25.64 589 66.23 5e-64 2-isopropylmalate synthase A OS Solanum pennellii GN IPMSA PE 2 SV 1
blastp_uniprot_sprot sp|Q39891|LEU1_SOYBN 5 153 + 149 none 26.37 565 61.74 4e-55 Probable 2-isopropylmalate synthase OS Glycine max GN GMN56 PE 2 SV 1
blastp_uniprot_sprot sp|P48576|LEU1_SYNY3 3 160 + 158 Gaps:14 27.02 533 44.44 3e-31 2-isopropylmalate synthase OS Synechocystis sp. (strain PCC 6803 / Kazusa) GN leuA PE 3 SV 1
blastp_uniprot_sprot sp|Q7NI93|LEU1_GLOVI 5 148 + 144 Gaps:14 24.16 538 46.15 1e-29 2-isopropylmalate synthase OS Gloeobacter violaceus (strain PCC 7421) GN leuA PE 3 SV 1
blastp_uniprot_sprot sp|Q7VBG1|LEU1_PROMA 3 149 + 147 Gaps:14 24.81 536 44.36 1e-28 2-isopropylmalate synthase OS Prochlorococcus marinus (strain SARG / CCMP1375 / SS120) GN leuA PE 3 SV 1
blastp_uniprot_sprot sp|B7KJX8|LEU1_CYAP7 3 167 + 165 Gaps:15 28.36 536 41.45 7e-28 2-isopropylmalate synthase OS Cyanothece sp. (strain PCC 7424) GN leuA PE 3 SV 1
blastp_uniprot_sprot sp|A2C3L7|LEU1_PROM1 3 149 + 147 Gaps:14 24.68 539 44.36 1e-27 2-isopropylmalate synthase OS Prochlorococcus marinus (strain NATL1A) GN leuA PE 3 SV 1
rpsblast_cdd gnl|CDD|177955 3 168 + 166 none 26.27 632 75.30 3e-68 PLN02321 PLN02321 2-isopropylmalate synthase.
rpsblast_cdd gnl|CDD|179166 4 154 + 151 Gaps:15 26.90 513 45.65 3e-32 PRK00915 PRK00915 2-isopropylmalate synthase Validated.
rpsblast_cdd gnl|CDD|203962 4 149 + 146 Gaps:15 100.00 131 44.27 6e-32 pfam08502 LeuA_dimer LeuA allosteric (dimerisation) domain. This is the C-terminal regulatory (R) domain of alpha-isopropylmalate synthase which catalyzes the first committed step in the leucine biosynthetic pathway. This domain is an internally duplicated structure with a novel fold. It comprises two similar units that are arranged such that the two -helices pack together in the centre crossing at an angle of 34 degrees sandwiched between the two three-stranded antiparallel beta-sheets. The overall domain is thus constructed as a beta-alpha-beta three-layer sandwich.
rpsblast_cdd gnl|CDD|197985 5 148 + 144 Gaps:16 99.24 131 40.77 2e-26 smart00917 LeuA_dimer LeuA allosteric (dimerisation) domain. This is the C-terminal regulatory (R) domain of alpha-isopropylmalate synthase which catalyses the first committed step in the leucine biosynthetic pathway. This domain is an internally duplicated structure with a novel fold. It comprises two similar units that are arranged such that the two -helices pack together in the centre crossing at an angle of 34 degrees sandwiched between the two three-stranded antiparallel beta-sheets. The overall domain is thus constructed as a beta-alpha-beta three-layer sandwich.
rpsblast_cdd gnl|CDD|130046 5 146 + 142 Gaps:16 25.91 494 48.44 2e-22 TIGR00973 leuA_bact 2-isopropylmalate synthase bacterial type. This is the first enzyme of leucine biosynthesis. A larger family of homologous proteins includes homocitrate synthase distinct lineages of 2-isopropylmalate synthase several distinct uncharacterized orthologous sets in the Archaea and other related enzymes. This model describes a family of 2-isopropylmalate synthases found primarily in Bacteria. The homologous families in the Archaea may represent isozymes and/or related enzymes.
rpsblast_cdd gnl|CDD|181814 1 149 + 149 Gaps:22 28.07 488 37.23 2e-14 PRK09389 PRK09389 (R)-citramalate synthase Provisional.
rpsblast_cdd gnl|CDD|179640 3 149 + 147 Gaps:23 25.36 552 30.00 4e-09 PRK03739 PRK03739 2-isopropylmalate synthase Validated.
rpsblast_kog gnl|CDD|37578 4 147 + 144 Gaps:7 24.46 560 34.31 1e-35 KOG2367 KOG2367 KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase [Amino acid transport and metabolism].

5 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 3 149 147 PTHR10277:SF9 none none none
Pfam 5 149 145 PF08502 "KEGG:00290+2.3.3.13","KEGG:00620+2.3.3.13","MetaCyc:PWY-6871","UniPathway:UPA00048" LeuA allosteric (dimerisation) domain IPR013709
PANTHER 3 149 147 PTHR10277 none none none
SMART 4 149 146 SM00917 "KEGG:00290+2.3.3.13","KEGG:00620+2.3.3.13","MetaCyc:PWY-6871","UniPathway:UPA00048" LeuA allosteric (dimerisation) domain IPR013709
SUPERFAMILY 26 148 123 SSF110921 "KEGG:00290+2.3.3.13","KEGG:00620+2.3.3.13","MetaCyc:PWY-6871","UniPathway:UPA00048" none IPR013709

0 Localization

1 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Champenoux_2015_nPriLBD_3P Qrob_Chr10 10 v_15000_157 v_15000_310 15,68 15,9 15,91 lod 2.4 5.5

0 Targeting