Protein : Qrob_P0564250.2 Q. robur

Protein Identifier  ? Qrob_P0564250.2 Organism . Name  Quercus robur
Score  87.0 Score Type  egn
Protein Description  (M=2) K03189 - urease accessory protein Gene Prediction Quality  validated
Protein length 

Sequence

Length: 269  
Kegg Orthology  K03189

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0 Synonyms

3 GO Terms

Identifier Name Description
GO:0003924 GTPase activity Catalysis of the reaction: GTP + H2O = GDP + phosphate.
GO:0016151 nickel cation binding Interacting selectively and non-covalently with nickel (Ni) cations.
GO:0006807 nitrogen compound metabolic process The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen, including (but not limited to) nitrogen fixation, nitrification, denitrification, assimilatory/dissimilatory nitrate reduction and the interconversion of nitrogenous organic matter and ammonium.

30 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|mdm:103434991 57 268 + 212 Gaps:22 69.09 275 95.79 2e-122 urease accessory protein G
blastp_kegg lcl|rcu:RCOM_0633220 57 268 + 212 Gaps:22 69.09 275 95.26 1e-121 Urease accessory protein ureG putative
blastp_kegg lcl|pop:POPTR_0002s24500g 57 268 + 212 Gaps:22 67.86 280 94.21 5e-121 POPTRDRAFT_645427 Ni-binding urease accessory protein UreG
blastp_kegg lcl|pper:PRUPE_ppa009642mg 57 268 + 212 Gaps:22 67.14 283 95.26 2e-120 hypothetical protein
blastp_kegg lcl|pmum:103325110 57 268 + 212 Gaps:22 67.14 283 95.26 2e-120 urease accessory protein G
blastp_kegg lcl|cit:102620030 57 267 + 211 Gaps:22 66.78 283 93.65 7e-120 urease accessory protein G-like
blastp_kegg lcl|cmo:103487531 57 268 + 212 Gaps:22 66.67 285 94.21 3e-119 urease accessory protein G
blastp_kegg lcl|tcc:TCM_029508 57 267 + 211 Gaps:22 60.97 310 95.24 3e-119 Urease accessory protein G
blastp_kegg lcl|fve:101296750 57 268 + 212 Gaps:22 68.35 278 94.74 3e-119 uncharacterized urease accessory protein ureG-like
blastp_kegg lcl|crb:CARUB_v10023840mg 57 268 + 212 Gaps:22 69.85 272 93.68 4e-119 hypothetical protein
blastp_pdb 2hf9_B 57 260 + 204 Gaps:35 76.55 226 35.84 5e-10 mol:protein length:226 Probable hydrogenase nickel incorporation pro
blastp_pdb 2hf9_A 57 260 + 204 Gaps:35 76.55 226 35.84 5e-10 mol:protein length:226 Probable hydrogenase nickel incorporation pro
blastp_pdb 2hf8_B 57 260 + 204 Gaps:35 76.55 226 35.84 5e-10 mol:protein length:226 Probable hydrogenase nickel incorporation pro
blastp_pdb 2hf8_A 57 260 + 204 Gaps:35 76.55 226 35.84 5e-10 mol:protein length:226 Probable hydrogenase nickel incorporation pro
blastp_pdb 2wsm_B 102 253 + 152 Gaps:13 63.80 221 34.04 5e-06 mol:protein length:221 HYDROGENASE EXPRESSION/FORMATION PROTEIN (HYP
blastp_pdb 2wsm_A 102 253 + 152 Gaps:13 63.80 221 34.04 5e-06 mol:protein length:221 HYDROGENASE EXPRESSION/FORMATION PROTEIN (HYP
blastp_uniprot_sprot sp|O64700|UREG_ARATH 57 268 + 212 Gaps:22 69.09 275 93.16 5e-120 Urease accessory protein G OS Arabidopsis thaliana GN UREG PE 2 SV 1
blastp_uniprot_sprot sp|E0ZS47|UREG_ORYSI 57 267 + 211 Gaps:22 66.55 284 92.59 5e-119 Urease accessory protein G OS Oryza sativa subsp. indica GN UREG PE 2 SV 1
blastp_uniprot_sprot sp|Q6AUF3|UREG_ORYSJ 57 268 + 212 Gaps:22 66.90 284 92.11 5e-118 Urease accessory protein G OS Oryza sativa subsp. japonica GN UREG PE 2 SV 1
blastp_uniprot_sprot sp|A7HHN3|UREG_ANADF 57 264 + 208 Gaps:22 78.81 236 74.19 6e-88 Urease accessory protein UreG OS Anaeromyxobacter sp. (strain Fw109-5) GN ureG PE 3 SV 1
blastp_uniprot_sprot sp|A9GP90|UREG_SORC5 57 263 + 207 Gaps:22 61.26 302 69.73 6e-79 Urease accessory protein UreG OS Sorangium cellulosum (strain So ce56) GN ureG PE 3 SV 1
blastp_uniprot_sprot sp|Q96WV0|UREG_SCHPO 98 262 + 165 none 57.69 286 71.52 1e-78 Uncharacterized urease accessory protein ureG-like OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN SPCPB16A4.05c PE 3 SV 1
blastp_uniprot_sprot sp|Q11VN1|UREG_CYTH3 57 262 + 206 Gaps:23 75.82 244 65.95 8e-75 Urease accessory protein UreG OS Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) GN ureG PE 3 SV 1
blastp_uniprot_sprot sp|B8IJ81|UREG_METNO 57 261 + 205 Gaps:23 90.20 204 64.67 3e-71 Urease accessory protein UreG OS Methylobacterium nodulans (strain ORS2060 / LMG 21967) GN ureG PE 3 SV 1
blastp_uniprot_sprot sp|B1M3X4|UREG1_METRJ 57 262 + 206 Gaps:23 90.24 205 62.16 4e-70 Urease accessory protein UreG 1 OS Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831) GN ureG1 PE 3 SV 1
blastp_uniprot_sprot sp|B0UBI3|UREG_METS4 57 261 + 205 Gaps:23 90.20 204 63.59 5e-70 Urease accessory protein UreG OS Methylobacterium sp. (strain 4-46) GN ureG PE 3 SV 1
rpsblast_cdd gnl|CDD|129208 57 255 + 199 Gaps:23 89.45 199 61.24 6e-63 TIGR00101 ureG urease accessory protein UreG. This model represents UreG a GTP hydrolase that acts in the assembly of the nickel metallocenter of urease. It is found only in urease-positive species although some urease-positive species (e.g. Bacillus subtilis) lack this protein. A similar protein hypB is an accessory protein for expression of hydrogenase which also uses nickel.
rpsblast_cdd gnl|CDD|30727 57 257 + 201 Gaps:32 85.64 202 50.29 4e-45 COG0378 HypB Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase [Posttranslational modification protein turnover chaperones / Transcription].
rpsblast_cdd gnl|CDD|202255 57 239 + 183 Gaps:43 93.26 178 36.14 5e-27 pfam02492 cobW CobW/HypB/UreG nucleotide-binding domain. This domain is found in HypB a hydrogenase expression / formation protein and UreG a urease accessory protein. Both these proteins contain a P-loop nucleotide binding motif. HypB has GTPase activity and is a guanine nucleotide binding protein. It is not known whether UreG binds GTP or some other nucleotide. Both enzymes are involved in nickel binding. HypB can store nickel and is required for nickel dependent hydrogenase expression. UreG is required for functional incorporation of the urease nickel metallocenter. GTP hydrolysis may required by these proteins for nickel incorporation into other nickel proteins. This family of domains also contains P47K a Pseudomonas chlororaphis protein needed for nitrile hydratase expression and the cobW gene product which may be involved in cobalamin biosynthesis in Pseudomonas denitrificans.
rpsblast_cdd gnl|CDD|188019 98 258 + 161 Gaps:17 73.08 208 34.21 3e-10 TIGR00073 hypB hydrogenase accessory protein HypB. A GTP hydrolase for assembly of nickel metallocenter of hydrogenase. A similar protein ureG is an accessory protein for urease which also uses nickel. hits scoring 75 and above are safe as orthologs.

9 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Phobius 56 268 213 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
SUPERFAMILY 98 260 163 SSF52540 none none IPR027417
TIGRFAM 97 256 160 TIGR00101 none ureG: urease accessory protein UreG IPR004400
Gene3D 115 258 144 G3DSA:3.40.50.300 none none IPR027417
Phobius 1 33 33 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 34 55 22 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
PANTHER 57 75 19 PTHR31715 none none IPR004400
PANTHER 98 263 166 PTHR31715 none none IPR004400
Pfam 98 237 140 PF02492 none CobW/HypB/UreG, nucleotide-binding domain IPR003495

1 Localization

Analysis Start End Length
TMHMM 34 56 22

1 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_rEpiBC_3P Qrob_Chr05 5 s_2GDU0O_517 s_1BT8O3_239 25,81 0 55,45 lod 2,2101 5,5

0 Targeting