Protein : Qrob_P0520310.2 Q. robur
Protein Identifier  ? Qrob_P0520310.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=1) 1.14.99.33 - Delta(12)-fatty acid dehydrogenase. Code Enzyme  EC:1.14.99.33
Gene Prediction Quality  validated Protein length 

Sequence

Length: 455  
Kegg Orthology  K10257
Paste the following link
Lists
This Protein isn't in any lists. Upload a list.

Sequence Feature Displayer

Protein Sequence Displayer

J Browse Displayer

0 Synonyms

3 GO Terms

Identifier Name Description
GO:0055114 oxidation-reduction process A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0006629 lipid metabolic process The chemical reactions and pathways involving lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent. Includes fatty acids; neutral fats, other fatty-acid esters, and soaps; long-chain (fatty) alcohols and waxes; sphingoids and other long-chain bases; glycolipids, phospholipids and sphingolipids; and carotenes, polyprenols, sterols, terpenes and other isoprenoids.
GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from each of two donors, and molecular oxygen is reduced to two molecules of water.

30 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pmum:103341784 2 452 + 451 Gaps:8 99.12 453 83.30 0.0 omega-3 fatty acid desaturase chloroplastic-like
blastp_kegg lcl|pper:PRUPE_ppa005574mg 2 452 + 451 Gaps:8 99.12 453 83.07 0.0 hypothetical protein
blastp_kegg lcl|pop:POPTR_0006s10250g 1 451 + 451 Gaps:12 99.56 451 83.52 0.0 POPTRDRAFT_831926 omega-3 desaturase family protein
blastp_kegg lcl|mdm:103430794 1 444 + 444 Gaps:7 97.79 453 82.39 0.0 omega-3 fatty acid desaturase chloroplastic-like
blastp_kegg lcl|tcc:TCM_021677 1 452 + 452 Gaps:5 100.00 455 82.20 0.0 Fatty acid desaturase 8 isoform 1
blastp_kegg lcl|pop:POPTR_0016s12490g 1 451 + 451 Gaps:12 99.34 452 81.74 0.0 POPTRDRAFT_825350 omega-3 desaturase family protein
blastp_kegg lcl|cit:102611986 1 452 + 452 Gaps:11 99.56 457 82.42 0.0 omega-3 fatty acid desaturase chloroplastic-like
blastp_kegg lcl|pxb:103966319 1 447 + 447 Gaps:7 98.45 453 80.94 0.0 omega-3 fatty acid desaturase chloroplastic-like
blastp_kegg lcl|mdm:103419381 1 444 + 444 Gaps:7 97.58 454 81.26 0.0 omega-3 fatty acid desaturase chloroplastic-like
blastp_kegg lcl|pvu:PHAVU_008G098100g 1 452 + 452 Gaps:3 99.56 453 78.94 0.0 hypothetical protein
blastp_uniprot_sprot sp|P48620|FAD3C_SESIN 1 452 + 452 Gaps:10 99.78 447 79.15 0.0 Omega-3 fatty acid desaturase chloroplastic OS Sesamum indicum GN FAD7 PE 2 SV 1
blastp_uniprot_sprot sp|P48619|FAD3C_RICCO 2 449 + 448 Gaps:9 98.04 460 74.72 0.0 Omega-3 fatty acid desaturase chloroplastic OS Ricinus communis GN FAD7A-1 PE 2 SV 1
blastp_uniprot_sprot sp|P48621|FAD3C_SOYBN 1 454 + 454 Gaps:3 100.00 453 77.70 0.0 Omega-3 fatty acid desaturase chloroplastic OS Glycine max GN FAD7 PE 2 SV 1
blastp_uniprot_sprot sp|P48622|FAD3D_ARATH 1 450 + 450 Gaps:17 99.54 435 73.90 0.0 Temperature-sensitive omega-3 fatty acid desaturase chloroplastic OS Arabidopsis thaliana GN FAD8 PE 2 SV 1
blastp_uniprot_sprot sp|P46310|FAD3C_ARATH 1 453 + 453 Gaps:24 99.33 446 72.46 0.0 Omega-3 fatty acid desaturase chloroplastic OS Arabidopsis thaliana GN FAD7 PE 1 SV 1
blastp_uniprot_sprot sp|P48618|FAD3C_BRANA 48 454 + 407 Gaps:22 97.77 404 77.72 0.0 Omega-3 fatty acid desaturase chloroplastic (Fragment) OS Brassica napus GN FAD7 PE 2 SV 1
blastp_uniprot_sprot sp|P48625|FAD3E_SOYBN 86 452 + 367 Gaps:1 96.32 380 72.40 0.0 Omega-3 fatty acid desaturase endoplasmic reticulum OS Glycine max GN FAD3 PE 2 SV 1
blastp_uniprot_sprot sp|P48624|FAD3E_BRANA 79 447 + 369 Gaps:2 95.82 383 73.30 0.0 Omega-3 fatty acid desaturase endoplasmic reticulum OS Brassica napus GN FAD3 PE 2 SV 1
blastp_uniprot_sprot sp|P32291|FAD3E_VIGRR 87 451 + 365 none 96.05 380 72.05 0.0 Omega-3 fatty acid desaturase endoplasmic reticulum OS Vigna radiata var. radiata GN ARG1 PE 2 SV 1
blastp_uniprot_sprot sp|P48623|FAD3E_ARATH 77 454 + 378 Gaps:5 98.70 386 71.13 0.0 Omega-3 fatty acid desaturase endoplasmic reticulum OS Arabidopsis thaliana GN FAD3 PE 2 SV 1
rpsblast_cdd gnl|CDD|178114 1 452 + 452 Gaps:8 99.56 450 82.81 0.0 PLN02498 PLN02498 omega-3 fatty acid desaturase.
rpsblast_cdd gnl|CDD|178121 81 406 + 326 Gaps:26 89.24 381 39.41 1e-74 PLN02505 PLN02505 omega-6 fatty acid desaturase.
rpsblast_cdd gnl|CDD|58173 122 390 + 269 Gaps:55 100.00 222 48.65 2e-59 cd03507 Delta12-FADS-like The Delta12 Fatty Acid Desaturase (Delta12-FADS)-like CD includes the integral-membrane enzymes delta-12 acyl-lipid desaturases oleate 12-hydroxylases omega3 and omega6 fatty acid desaturases and other related proteins found in a wide range of organisms including higher plants green algae diatoms nematodes fungi and bacteria. The expression of these proteins appears to be temperature dependent: decreases in temperature result in increased levels of fatty acid desaturation within membrane lipids subsequently altering cell membrane fluidity. An important enzyme for the production of polyunsaturates in plants is the oleate delta-12 desaturase (Arabidopsis FAD2) of the endoplasmic reticulum. This enzyme accepts l-acyl-2-oleoyl-sn-glycero-3-phosphocholine as substrate and requires NADH:cytochrome b oxidoreductase cytochrome b and oxygen for activity. FAD2 converts oleate(18:1) to linoleate (18:2) and is closely related to oleate 12-hydroxylase which catalyzes the hydroxylation of oleate to ricinoleate. Plastid-bound desaturases (Arabidopsis delta-12 desaturase (FAD6) omega-3 desaturase (FAD8) omega-6 desaturase (FAD6)) as well as the cyanobacterial thylakoid-bound FADSs require oxygen ferredoxin and ferredoxin oxidoreductase for activity. As in higher plants the cyanobacteria delta-12 (DesA) and omega-3 (DesB) FADSs desaturate oleate (18:1) to linoleate (18:2) and linoleate (18:2) to linolenate (18:3) respectively. Omega-3 (DesB/FAD8) and omega-6 (DesD/FAD6) desaturases catalyze reactions that introduce a double bond between carbons three and four and carbons six and seven respectively from the methyl end of fatty acids. As with other members of this superfamily this domain family has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain eight histidine residues: HXXXH HXX(X)HH and HXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the homologue stearoyl CoA desaturase. Mutation of any one of four of these histidines in the Synechocystis delta-12 acyl-lipid desaturase resulted in complete inactivity..
rpsblast_cdd gnl|CDD|152395 1 143 + 143 Gaps:10 100.00 135 65.93 2e-59 pfam11960 DUF3474 Domain of unknown function (DUF3474). This presumed domain is functionally uncharacterized. This domain is found in bacteria and eukaryotes. This domain is typically between 126 to 140 amino acids in length. This domain is found associated with pfam00487.
rpsblast_cdd gnl|CDD|33051 104 404 + 301 Gaps:19 83.38 343 28.67 6e-41 COG3239 DesA Fatty acid desaturase [Lipid metabolism].
rpsblast_cdd gnl|CDD|178208 103 414 + 312 Gaps:19 72.45 421 30.82 2e-36 PLN02598 PLN02598 omega-6 fatty acid desaturase.
rpsblast_cdd gnl|CDD|201259 150 405 + 256 Gaps:13 96.81 251 23.05 9e-28 pfam00487 FA_desaturase Fatty acid desaturase.
rpsblast_cdd gnl|CDD|58170 151 226 + 76 Gaps:3 59.84 122 31.51 2e-10 cd01060 Membrane-FADS-like The membrane fatty acid desaturase (Membrane_FADS)-like CD includes membrane FADSs alkane hydroxylases beta carotene ketolases (CrtW-like) hydroxylases (CrtR-like) and other related proteins. They are present in all groups of organisms with the exception of archaea. Membrane FADSs are non-heme iron-containing oxygen-dependent enzymes involved in regioselective introduction of double bonds in fatty acyl aliphatic chains. They play an important role in the maintenance of the proper structure and functioning of biological membranes. Alkane hydroxylases are bacterial integral-membrane di-iron enzymes that share a requirement for iron and oxygen for activity similar to that of membrane FADSs and are involved in the initial oxidation of inactivated alkanes. Beta-carotene ketolase and beta-carotene hydroxylase are carotenoid biosynthetic enzymes for astaxanthin and zeaxanthin respectively. This superfamily domain has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of these sequences also reveals three regions of conserved histidine cluster motifs that contain eight histidine residues: HXXX(X)H HXX(X)HH and HXXHH (an additional conserved histidine residue is seen between clusters 2 and 3). Spectroscopic and genetic evidence point to a nitrogen-rich coordination environment located in the cytoplasm with as many as eight histidines coordinating the two iron ions and a carboxylate residue bridging the two metals in the Pseudomonas oleovorans alkane hydroxylase (AlkB). In addition the eight histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the rat stearoyl CoA delta-9 desaturase..
rpsblast_cdd gnl|CDD|58172 162 405 + 244 Gaps:50 96.08 204 21.94 5e-09 cd03506 Delta6-FADS-like The Delta6 Fatty Acid Desaturase (Delta6-FADS)-like CD includes the integral-membrane enzymes: delta-4 delta-5 delta-6 delta-8 delta-8-sphingolipid and delta-11 desaturases found in vertebrates higher plants fungi and bacteria. These desaturases are required for the synthesis of highly unsaturated fatty acids (HUFAs) which are mainly esterified into phospholipids and contribute to maintaining membrane fluidity. While HUFAs may be required for cold tolerance in bacteria plants and fish the primary role of HUFAs in mammals is cell signaling. These enzymes are described as front-end desaturases because they introduce a double bond between the pre-exiting double bond and the carboxyl (front) end of the fatty acid. Various substrates are involved with both acyl-coenzyme A (CoA) and acyl-lipid desaturases present in this CD. Acyl-lipid desaturases are localized in the membranes of cyanobacterial thylakoid plant endoplasmic reticulum (ER) and plastid and acyl-CoA desaturases are present in ER membrane. ER-bound plant acyl-lipid desaturases and acyl-CoA desaturases require cytochrome b5 as an electron donor. Most of the eukaryotic desaturase domains have an adjacent N-terminal cytochrome b5-like domain. This domain family has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain the residues: HXXXH HXX(X)HH and Q/HXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the homolog stearoyl CoA desaturase..
rpsblast_cdd gnl|CDD|58177 126 406 + 281 Gaps:44 93.68 285 24.72 1e-08 cd03511 Rhizopine-oxygenase-like This CD includes the putative hydrocarbon oxygenase MocD a bacterial rhizopine (3-O-methyl-scyllo-inosamine 3-O-MSI) oxygenase and other related proteins. It has been proposed that MocD MocE (Rieske-like ferredoxin) and MocF (ferredoxin reductase) under the regulation of MocR act in concert to form a ferredoxin oxygenase system that demethylates 3-O-MSI to form scyllo-inosamine. This domain family appears to be structurally related to the membrane fatty acid desaturases and the alkane hydroxylases. They all share in common extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain eight histidine residues: HXXXH HXXHH and HXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within homologs stearoyl CoA desaturase and alkane hydroxylase..

13 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Phobius 333 454 122 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 130 148 19 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 312 332 21 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Pfam 150 405 256 PF00487 none Fatty acid desaturase IPR005804
Phobius 1 129 129 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
PANTHER 91 452 362 PTHR32100 none none none
Phobius 154 172 19 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
PANTHER 91 452 362 PTHR32100:SF12 none none none
Phobius 149 153 5 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Pfam 1 143 143 PF11960 "UniPathway:UPA00658" Domain of unknown function (DUF3474) IPR021863
Phobius 285 306 22 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 173 284 112 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 307 311 5 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none

3 Localization

Analysis Start End Length
TMHMM 285 307 22
TMHMM 314 336 22
TMHMM 136 158 22

5 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nSecLBD_A4 Qrob_Chr07 7 v_8327_222 s_1A4WGY_363 16,04 0 44,69 lod 2,6373 6,5
Bourran2_2014_nP_A4 Qrob_Chr11 11 s_1B58GB_1413 s_1A5BYY_1671 11,15 0 42,38 lod 1,8913 4,5
Bourran2_2015_nSeqBC_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,06 25,47 27,72 lod 3.6 7.1
Bourran2_2015_nEpis_A4 Qrob_Chr09 9 v_15847_485 v_8329_369 34,94 34,88 37,45 lod 3.1 7
Bourran2_2015_nEpiBC_A4 Qrob_Chr07 7 s_1DP9TW_798 v_8128_173 22,61 22,14 22,73 lod 3.1 8.5

0 Targeting