Protein : Qrob_P0497650.2 Q. robur
Protein Identifier  ? Qrob_P0497650.2 Organism . Name  Quercus robur
Score  81.4 Score Type  egn
Protein Description  (M=1) 2.7.1.160 - 2'-phosphotransferase. Code Enzyme  EC:2.7.1.160
Gene Prediction Quality  validated Protein length 

Sequence

Length: 243  
Kegg Orthology  K10669
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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0016772 transferase activity, transferring phosphorus-containing groups Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
GO:0006388 tRNA splicing, via endonucleolytic cleavage and ligation Splicing of tRNA substrates via recognition of the folded RNA structure that brings the 5' and 3' splice sites into proximity and cleavage of the RNA at both the 3' and 5' splice sites by an endonucleolytic mechanism, followed by ligation of the exons.

25 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pvu:PHAVU_010G084500g 5 236 + 232 Gaps:2 80.14 292 85.04 1e-134 hypothetical protein
blastp_kegg lcl|gmx:100805163 3 236 + 234 Gaps:2 80.55 293 83.90 1e-134 tRNA 2'-phosphotransferase 1-like
blastp_kegg lcl|cit:102609292 4 236 + 233 none 77.93 299 83.69 7e-134 tRNA 2'-phosphotransferase 1-like
blastp_kegg lcl|csv:101214846 4 236 + 233 Gaps:3 92.91 254 83.05 2e-133 putative tRNA 2'-phosphotransferase-like
blastp_kegg lcl|vvi:100254014 28 236 + 209 none 70.37 297 89.00 2e-133 tRNA 2'-phosphotransferase 1-like
blastp_kegg lcl|cmo:103483358 4 236 + 233 Gaps:3 80.55 293 83.47 1e-132 tRNA 2'-phosphotransferase 1
blastp_kegg lcl|crb:CARUB_v10023776mg 3 236 + 234 Gaps:1 80.90 288 78.97 2e-132 hypothetical protein
blastp_kegg lcl|tcc:TCM_005527 3 236 + 234 Gaps:1 79.93 294 86.38 3e-132 RNA 2'-phosphotransferase isoform 1
blastp_kegg lcl|vvi:100245349 28 236 + 209 none 69.44 301 89.00 4e-132 tRNA 2'-phosphotransferase 1-like
blastp_kegg lcl|sly:101250158 10 234 + 225 none 75.00 300 82.22 8e-130 tRNA 2'-phosphotransferase 1-like
blastp_uniprot_sprot sp|Q8K3A2|TRPT1_MOUSE 28 234 + 207 Gaps:14 81.53 249 41.87 4e-39 tRNA 2'-phosphotransferase 1 OS Mus musculus GN Trpt1 PE 2 SV 2
blastp_uniprot_sprot sp|Q5EAR5|TRPT1_DANRE 34 226 + 193 Gaps:12 84.00 225 40.21 2e-37 tRNA 2'-phosphotransferase 1 OS Danio rerio GN trpt1 PE 2 SV 2
blastp_uniprot_sprot sp|Q3ZBM7|TRPT1_BOVIN 28 234 + 207 Gaps:14 79.92 254 40.89 1e-36 tRNA 2'-phosphotransferase 1 OS Bos taurus GN TRPT1 PE 2 SV 3
blastp_uniprot_sprot sp|O14045|TPT1_SCHPO 36 242 + 207 Gaps:14 55.62 365 37.93 1e-34 Putative tRNA 2'-phosphotransferase OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN SPAC2C4.12c PE 3 SV 2
blastp_uniprot_sprot sp|Q86TN4|TRPT1_HUMAN 2 234 + 233 Gaps:26 85.77 253 40.09 3e-34 tRNA 2'-phosphotransferase 1 OS Homo sapiens GN TRPT1 PE 1 SV 2
blastp_uniprot_sprot sp|Q12272|TPT1_YEAST 28 225 + 198 Gaps:25 84.78 230 37.95 7e-23 tRNA 2'-phosphotransferase OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN TPT1 PE 1 SV 1
blastp_uniprot_sprot sp|Q8R5N7|KPTA_FUSNN 34 221 + 188 Gaps:20 94.97 179 36.47 3e-18 Probable RNA 2'-phosphotransferase OS Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131) GN kptA PE 3 SV 1
blastp_uniprot_sprot sp|Q0TUT1|KPTA_CLOP1 34 225 + 192 Gaps:25 93.01 186 37.57 3e-18 Probable RNA 2'-phosphotransferase OS Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) GN kptA PE 3 SV 1
blastp_uniprot_sprot sp|B0C090|KPTA_ACAM1 30 226 + 197 Gaps:23 98.34 181 34.83 1e-17 Probable RNA 2'-phosphotransferase OS Acaryochloris marina (strain MBIC 11017) GN kptA PE 3 SV 1
blastp_uniprot_sprot sp|B2IZ22|KPTA_NOSP7 34 225 + 192 Gaps:19 95.58 181 32.95 2e-17 Probable RNA 2'-phosphotransferase OS Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN kptA PE 3 SV 1
rpsblast_cdd gnl|CDD|202028 34 211 + 178 Gaps:20 97.04 169 42.68 6e-47 pfam01885 PTS_2-RNA RNA 2'-phosphotransferase Tpt1 / KptA family. Tpt1 catalyzes the last step of tRNA splicing in yeast. It transfers the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1"-2"-cyclic phosphate. This is presumed to be followed by a transesterification step to release the RNA. The first step of this reaction is similar to that catalyzed by some bacterial toxins. E. coli KptA and mouse Tpt1 are likely to use the same reaction mechanism.
rpsblast_cdd gnl|CDD|185551 28 233 + 206 Gaps:15 34.54 582 41.29 5e-39 PTZ00315 PTZ00315 2'-phosphotransferase Provisional.
rpsblast_cdd gnl|CDD|32044 7 225 + 219 Gaps:22 95.26 211 31.84 2e-25 COG1859 KptA RNA:NAD 2'-phosphotransferase [Translation ribosomal structure and biogenesis].
rpsblast_cdd gnl|CDD|179136 34 225 + 192 Gaps:33 95.53 179 35.09 4e-25 PRK00819 PRK00819 RNA 2'-phosphotransferase Reviewed.
rpsblast_kog gnl|CDD|37489 25 236 + 212 Gaps:5 100.00 207 64.25 7e-79 KOG2278 KOG2278 KOG2278 RNA:NAD 2'-phosphotransferase TPT1 [Translation ribosomal structure and biogenesis].

4 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
SUPERFAMILY 34 215 182 SSF56399 none none none
PANTHER 9 239 231 PTHR12684:SF2 none none none
PANTHER 9 239 231 PTHR12684 none none none
Pfam 30 216 187 PF01885 none RNA 2'-phosphotransferase, Tpt1 / KptA family IPR002745

0 Localization

8 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nLBD*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 34,91 16,12 53,62 lod 2,4961 5,2
Bourran2_2014_rEpiBC*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 35,77 14,11 55,31 lod 2,9413 6,2
Bourran2_2015_nEpiBC_3P Qrob_Chr12 12 s_1B73S5_217 v_7050_211 28,31 26,37 28,45 lod 4.5 11.6
Bourran2_2014_aSeqBC_A4 Qrob_Chr08 8 v_15999_278 v_AP13YL15_395 32,52 4,22 57,22 lod 2,7561 6,7
Bourran2_2014_nFork*_A4 Qrob_Chr08 8 PIE175 s_1CD7GJ_1398 31,22 5,24 57,24 lod 2,6724 6,8
Bourran2_2015_rEpiBC_3P Qrob_Chr08 8 s_A9TNV_543 v_11837_70 9,93 9,83 11,15 lod 3.3 7.3
Champenoux_2015_nSeqBC_A4 Qrob_Chr08 8 v_AD7YD13_501 s_1A7IED_780 43,44 43,42 43,99 lod 3.7 8.9
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL3_d13Cf Qrob_Chr08 8 v_5216_549 v_11625_20 37.08 12,26 54,9 lod 6.5888 0.04

0 Targeting