Protein : Qrob_P0497530.2 Q. robur
Protein Identifier  ? Qrob_P0497530.2 Organism . Name  Quercus robur
Score  0.0 Score Type  egn
Protein Description  (M=2) 2.1.1.72 - Site-specific DNA-methyltransferase (adenine-specific). Code Enzyme  EC:2.1.1.72
Gene Prediction Quality  validated Protein length 

Sequence

Length: 259  
Kegg Orthology  K19589
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0003676 nucleic acid binding Interacting selectively and non-covalently with any nucleic acid.
GO:0008168 methyltransferase activity Catalysis of the transfer of a methyl group to an acceptor molecule.
GO:0032259 methylation The process in which a methyl group is covalently attached to a molecule.
GO:0008276 protein methyltransferase activity Catalysis of the transfer of a methyl group (CH3-) to a protein.

32 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|csv:101224375 3 257 + 255 Gaps:3 98.44 256 80.16 5e-143 hemK methyltransferase family member 2-like
blastp_kegg lcl|csv:101206675 3 257 + 255 Gaps:3 98.44 256 80.16 5e-143 hemK methyltransferase family member 2-like
blastp_kegg lcl|cic:CICLE_v10016345mg 1 258 + 258 Gaps:2 100.00 256 77.73 2e-142 hypothetical protein
blastp_kegg lcl|cmo:103495363 3 255 + 253 Gaps:3 97.66 256 80.40 4e-142 hemK methyltransferase family member 2
blastp_kegg lcl|pmum:103325545 1 256 + 256 Gaps:5 99.60 252 80.08 1e-141 hemK methyltransferase family member 2
blastp_kegg lcl|cit:102617336 1 258 + 258 Gaps:2 100.00 256 77.34 3e-141 hemK methyltransferase family member 2-like
blastp_kegg lcl|pper:PRUPE_ppa018312mg 1 256 + 256 Gaps:5 99.60 252 79.68 2e-140 hypothetical protein
blastp_kegg lcl|fve:101294148 1 258 + 258 Gaps:5 100.00 253 78.26 9e-140 hemK methyltransferase family member 2-like
blastp_kegg lcl|rcu:RCOM_0096960 1 257 + 257 Gaps:1 100.00 256 78.12 8e-139 n6-DNA-methyltransferase putative
blastp_kegg lcl|tcc:TCM_021198 1 258 + 258 Gaps:1 98.47 261 75.10 2e-138 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
blastp_pdb 3q87_B 18 210 + 193 Gaps:36 92.35 170 37.58 5e-16 mol:protein length:170 N6 adenine specific DNA methylase
blastp_uniprot_sprot sp|Q9Y5N5|HEMK2_HUMAN 16 213 + 198 Gaps:5 90.19 214 45.08 3e-44 HemK methyltransferase family member 2 OS Homo sapiens GN N6AMT1 PE 1 SV 3
blastp_uniprot_sprot sp|Q9UT94|MTQ2_SCHPO 6 213 + 208 Gaps:16 93.51 231 33.33 7e-27 eRF1 methyltransferase catalytic subunit mtq2 OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN mtq2 PE 3 SV 1
blastp_uniprot_sprot sp|Q58338|Y928_METJA 1 213 + 213 Gaps:17 99.49 197 32.65 6e-23 Putative protein methyltransferase MJ0928 OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) GN MJ0928 PE 3 SV 1
blastp_uniprot_sprot sp|Q03920|MTQ2_YEAST 14 213 + 200 Gaps:21 95.48 221 29.38 1e-15 eRF1 methyltransferase catalytic subunit MTQ2 OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN MTQ2 PE 1 SV 1
blastp_uniprot_sprot sp|Q748B2|PRMC_GEOSL 42 198 + 157 Gaps:17 54.23 284 27.92 2e-07 Release factor glutamine methyltransferase OS Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) GN prmC PE 3 SV 1
blastp_uniprot_sprot sp|O28490|Y1784_ARCFU 47 213 + 167 Gaps:19 84.75 177 32.67 6e-07 Putative protein methyltransferase AF_1784 OS Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) GN AF_1784 PE 3 SV 1
blastp_uniprot_sprot sp|Q9Y5R4|HEMK1_HUMAN 45 199 + 155 Gaps:16 47.63 338 31.68 1e-06 HemK methyltransferase family member 1 OS Homo sapiens GN HEMK1 PE 2 SV 1
blastp_uniprot_sprot sp|O66506|PRMC_AQUAE 47 169 + 123 Gaps:17 43.42 281 35.25 4e-06 Release factor glutamine methyltransferase OS Aquifex aeolicus (strain VF5) GN prmC PE 3 SV 1
blastp_uniprot_sprot sp|A0R213|PRMC_MYCS2 59 167 + 109 Gaps:6 39.50 281 35.14 8e-06 Release factor glutamine methyltransferase OS Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) GN prmC PE 3 SV 1
rpsblast_cdd gnl|CDD|184932 14 213 + 200 Gaps:23 99.47 188 29.95 6e-29 PRK14968 PRK14968 putative methyltransferase Provisional.
rpsblast_cdd gnl|CDD|129628 17 212 + 196 Gaps:17 100.00 179 32.96 2e-23 TIGR00537 hemK_rel_arch HemK-related putative methylase. The gene hemK from E. coli was found to contribute to heme biosynthesis and originally suggested to be protoporphyrinogen oxidase. Functional analysis of the nearest homolog in Saccharomyces cerevisiae YNL063w finds it is not protoporphyrinogen oxidase and sequence analysis suggests that HemK homologs have S-adenosyl-methionine-dependent methyltransferase activity. Homologs are found usually in a single copy in nearly all completed genomes but varying somewhat in apparent domain architecture. This model represents an archaeal and eukaryotic protein family that lacks an N-terminal domain found in HemK and its eubacterial homologs. It is found in a single copy in the first six completed archaeal and eukaryotic genomes.
rpsblast_cdd gnl|CDD|32715 15 171 + 157 Gaps:17 53.57 280 32.67 6e-22 COG2890 HemK Methylase of polypeptide chain release factors [Translation ribosomal structure and biogenesis].
rpsblast_cdd gnl|CDD|184931 15 200 + 186 Gaps:12 78.03 223 32.76 8e-19 PRK14967 PRK14967 putative methyltransferase Provisional.
rpsblast_cdd gnl|CDD|200288 15 171 + 157 Gaps:21 60.56 251 32.89 2e-14 TIGR03534 RF_mod_PrmC protein-(glutamine-N5) methyltransferase release factor-specific. Members of this protein family are HemK (PrmC) a protein once thought to be involved in heme biosynthesis but now recognized to be a protein-glutamine methyltransferase that modifies the peptide chain release factors. All members of the seed alignment are encoded next to the release factor 1 gene (prfA) and confirmed by phylogenetic analysis. SIMBAL analysis (manuscript in prep.) shows the motif [LIV]PRx[DE]TE (in Escherichia coli IPRPDTE) confers specificity for the release factors rather than for ribosomal protein L3.
rpsblast_cdd gnl|CDD|181780 27 171 + 145 Gaps:18 51.27 275 30.50 3e-13 PRK09328 PRK09328 N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase Provisional.
rpsblast_cdd gnl|CDD|205836 43 173 + 131 Gaps:20 98.29 117 27.83 7e-10 pfam13659 Methyltransf_26 Methyltransferase domain. This family contains methyltransferase domains.
rpsblast_cdd gnl|CDD|203189 13 173 + 161 Gaps:34 79.41 170 27.41 3e-09 pfam05175 MTS Methyltransferase small domain. This domain is found in ribosomal RNA small subunit methyltransferase C as well as other methyltransferases .
rpsblast_cdd gnl|CDD|129627 26 169 + 144 Gaps:17 49.65 284 31.21 3e-08 TIGR00536 hemK_fam HemK family putative methylases. The gene hemK from E. coli was found to contribute to heme biosynthesis and originally suggested to be protoporphyrinogen oxidase. Functional analysis of the nearest homolog in Saccharomyces cerevisiae YNL063w finds it is not protoporphyrinogen oxidase and sequence analysis suggests that HemK homologs have S-adenosyl-methionine-dependent methyltransferase activity. Homologs are found usually in a single copy in nearly all completed genomes but varying somewhat in apparent domain architecture. Both E. coli and H. influenzae have two members rather than one. The members from the Mycoplasmas have an additional C-terminal domain.
rpsblast_cdd gnl|CDD|163416 11 209 + 199 Gaps:25 76.49 251 27.60 3e-07 TIGR03704 PrmC_rel_meth putative protein-(glutamine-N5) methyltransferase unknown substrate-specific. This protein family is closely related to two different families of protein-(glutamine-N5) methyltransferase. The first is PrmB which modifies ribosomal protein L3 in some bacteria. The second is PrmC (HemK) which modifies peptide chain release factors 1 and 2 in most bacteria and also in eukaryotes. The glutamine side chain-binding motif NPPY shared by PrmB and PrmC is N[VAT]PY in this family. The protein substrate is unknown.

7 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 4 234 231 PTHR18895 none none none
SUPERFAMILY 14 200 187 SSF53335 none none IPR029063
TIGRFAM 17 212 196 TIGR00537 none hemK_rel_arch: putative methylase IPR004557
PANTHER 4 234 231 PTHR18895:SF64 none none none
Pfam 45 173 129 PF13659 none Methyltransferase domain none
Gene3D 15 202 188 G3DSA:3.40.50.150 none none IPR029063
ProSitePatterns 119 125 7 PS00092 none N-6 Adenine-specific DNA methylases signature. IPR002052

0 Localization

8 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nLBD*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 34,91 16,12 53,62 lod 2,4961 5,2
Bourran2_2014_rEpiBC*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 35,77 14,11 55,31 lod 2,9413 6,2
Bourran2_2015_nEpiBC_3P Qrob_Chr12 12 s_1B73S5_217 v_7050_211 28,31 26,37 28,45 lod 4.5 11.6
Bourran2_2014_aSeqBC_A4 Qrob_Chr08 8 v_15999_278 v_AP13YL15_395 32,52 4,22 57,22 lod 2,7561 6,7
Bourran2_2014_nFork*_A4 Qrob_Chr08 8 PIE175 s_1CD7GJ_1398 31,22 5,24 57,24 lod 2,6724 6,8
Bourran2_2015_rEpiBC_3P Qrob_Chr08 8 s_A9TNV_543 v_11837_70 9,93 9,83 11,15 lod 3.3 7.3
Champenoux_2015_nSeqBC_A4 Qrob_Chr08 8 v_AD7YD13_501 s_1A7IED_780 43,44 43,42 43,99 lod 3.7 8.9
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL3_d13Cf Qrob_Chr08 8 v_5216_549 v_11625_20 37.08 12,26 54,9 lod 6.5888 0.04

0 Targeting