Protein : Qrob_P0455120.2 Q. robur
Protein Identifier  ? Qrob_P0455120.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=4) 5.4.99.28 - tRNA pseudouridine(32) synthase. Code Enzyme  EC:5.4.99.28
Gene Prediction Quality  validated Protein length 

Sequence

Length: 156  
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0003723 RNA binding Interacting selectively and non-covalently with an RNA molecule or a portion thereof.
GO:0001522 pseudouridine synthesis The intramolecular conversion of uridine to pseudouridine within an RNA molecule. This posttranscriptional base modification occurs in tRNA, rRNA, and snRNAs.
GO:0009451 RNA modification The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically.
GO:0009982 pseudouridine synthase activity Catalysis of the reaction: RNA uridine = RNA pseudouridine. Conversion of uridine in an RNA molecule to pseudouridine by rotation of the C1'-N-1 glycosidic bond of uridine in RNA to a C1'-C5.

38 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|cic:CICLE_v10033672mg 1 128 + 128 Gaps:2 41.03 312 81.25 1e-63 hypothetical protein
blastp_kegg lcl|tcc:TCM_046955 1 123 + 123 none 32.54 378 78.05 9e-62 Pseudouridine synthase family protein
blastp_kegg lcl|pper:PRUPE_ppa012152mg 1 123 + 123 none 67.96 181 77.24 1e-61 hypothetical protein
blastp_kegg lcl|cit:102607668 1 128 + 128 Gaps:3 33.08 390 80.62 3e-61 RNA pseudouridine synthase 5-like
blastp_kegg lcl|eus:EUTSA_v10010446mg 2 123 + 122 Gaps:1 32.20 382 76.42 5e-60 hypothetical protein
blastp_kegg lcl|pmum:103332388 1 123 + 123 none 31.30 393 77.24 8e-60 RNA pseudouridine synthase 5
blastp_kegg lcl|pop:POPTR_0008s04160g 1 125 + 125 Gaps:1 33.42 377 76.19 4e-59 POPTRDRAFT_873868 hypothetical protein
blastp_kegg lcl|mdm:103444808 1 123 + 123 none 31.87 386 76.42 9e-58 RNA pseudouridine synthase 5
blastp_kegg lcl|vvi:100265032 1 123 + 123 none 35.34 348 73.98 1e-57 RNA pseudourine synthase 5-like
blastp_kegg lcl|fve:101302785 6 123 + 118 none 31.47 375 76.27 1e-57 RNA pseudourine synthase 5-like
blastp_pdb 1prz_A 1 107 + 107 Gaps:8 39.29 252 36.36 3e-10 mol:protein length:252 Ribosomal large subunit pseudouridine synthas
blastp_pdb 2ist_A 1 107 + 107 Gaps:8 30.46 325 36.36 5e-10 mol:protein length:325 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1v9f_A 1 107 + 107 Gaps:8 30.46 325 36.36 5e-10 mol:protein length:325 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1qyu_A 1 107 + 107 Gaps:8 28.37 349 36.36 7e-10 mol:protein length:349 Ribosomal large subunit pseudouridine synthas
blastp_pdb 2i82_D 7 103 + 97 Gaps:3 43.32 217 34.04 8e-07 mol:protein length:217 Ribosomal large subunit pseudouridine synthas
blastp_pdb 2i82_C 7 103 + 97 Gaps:3 43.32 217 34.04 8e-07 mol:protein length:217 Ribosomal large subunit pseudouridine synthas
blastp_pdb 2i82_B 7 103 + 97 Gaps:3 43.32 217 34.04 8e-07 mol:protein length:217 Ribosomal large subunit pseudouridine synthas
blastp_pdb 2i82_A 7 103 + 97 Gaps:3 43.32 217 34.04 8e-07 mol:protein length:217 Ribosomal large subunit pseudouridine synthas
blastp_uniprot_sprot sp|Q5M721|PUS5_ARATH 2 123 + 122 Gaps:3 33.88 369 69.60 3e-55 RNA pseudouridine synthase 5 OS Arabidopsis thaliana GN At3g52260 PE 2 SV 2
blastp_uniprot_sprot sp|Q0DST9|PUS5_ORYSJ 2 128 + 127 Gaps:3 32.75 397 64.62 9e-48 RNA pseudouridine synthase 5 OS Oryza sativa subsp. japonica GN Os03g0288500 PE 2 SV 2
blastp_uniprot_sprot sp|P72970|Y1592_SYNY3 11 125 + 115 Gaps:4 38.83 291 45.13 5e-19 Uncharacterized RNA pseudouridine synthase slr1592 OS Synechocystis sp. (strain PCC 6803 / Kazusa) GN slr1592 PE 3 SV 1
blastp_uniprot_sprot sp|O67638|Y1758_AQUAE 7 101 + 95 Gaps:3 29.11 316 45.65 4e-15 Uncharacterized RNA pseudouridine synthase aq_1758 OS Aquifex aeolicus (strain VF5) GN aq_1758 PE 3 SV 1
blastp_uniprot_sprot sp|P54604|YHCT_BACSU 7 106 + 100 Gaps:3 32.12 302 37.11 4e-13 Uncharacterized RNA pseudouridine synthase YhcT OS Bacillus subtilis (strain 168) GN yhcT PE 3 SV 2
blastp_uniprot_sprot sp|Q9L7A7|RLUD_HAEDU 7 107 + 101 Gaps:8 28.62 325 40.86 2e-12 Ribosomal large subunit pseudouridine synthase D OS Haemophilus ducreyi (strain 35000HP / ATCC 700724) GN rluD PE 3 SV 2
blastp_uniprot_sprot sp|Q82WZ5|RLUD_NITEU 7 117 + 111 Gaps:10 29.36 344 43.56 2e-12 Ribosomal large subunit pseudouridine synthase D OS Nitrosomonas europaea (strain ATCC 19718 / NBRC 14298) GN rluD PE 3 SV 1
blastp_uniprot_sprot sp|Q9CKA6|RLUD_PASMU 7 107 + 101 Gaps:8 28.70 324 41.94 3e-12 Ribosomal large subunit pseudouridine synthase D OS Pasteurella multocida (strain Pm70) GN rluD PE 3 SV 1
blastp_uniprot_sprot sp|Q45480|YLYB_BACSU 6 101 + 96 Gaps:5 30.03 303 39.56 5e-12 Uncharacterized RNA pseudouridine synthase YlyB OS Bacillus subtilis (strain 168) GN ylyB PE 3 SV 3
blastp_uniprot_sprot sp|P33640|RLUD_PSEAE 6 103 + 98 Gaps:6 28.75 320 41.30 5e-12 Ribosomal large subunit pseudouridine synthase D OS Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) GN rluD PE 3 SV 2
rpsblast_cdd gnl|CDD|211346 7 101 + 95 Gaps:4 49.19 185 50.55 7e-25 cd02869 PseudoU_synth_RluCD_like Pseudouridine synthase RsuA/RluD family. This group is comprised of eukaryotic bacterial and archeal proteins similar to eight site specific Escherichia coli pseudouridine synthases: RsuA RluA RluB RluC RluD RluE RluF and TruA. Pseudouridine synthases catalyze the isomerization of specific uridines in a n RNA molecule to pseudouridines (5-ribosyluracil psi) requiring no cofactors. E. coli RluC for example makes psi955 2504 and 2580 in 23S RNA. Some psi sites such as psi1917 in 23S RNA made by RluD are universally conserved. Other psi sites occur in a more restricted fashion for example psi2819 in 21S mitochondrial ribosomal RNA made by S. cerevisiae Pus5p is only found in mitochondrial large subunit rRNAs from some other species and in gram negative bacteria. The E. coli counterpart of this psi residue is psi2580 in 23S rRNA. psi2604in 23S RNA made by RluF has only been detected in E.coli.
rpsblast_cdd gnl|CDD|161659 7 107 + 101 Gaps:3 32.78 299 45.92 3e-24 TIGR00005 rluA_subfam pseudouridine synthase RluA family. In E. coli RluD (SfhB) modifies uridine to pseudouridine at 23S RNA U1911 1915 and 1917 RluC modifies 955 2504 and 2580 and RluA modifies U746 and tRNA U32. An additional homolog from E. coli outside this family TruC modifies uracil-65 in transfer RNAs to pseudouridine.

4 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Pfam 6 89 84 PF00849 none RNA pseudouridylate synthase IPR006145
PANTHER 10 126 117 PTHR10436:SF93 none none IPR028766
PANTHER 10 126 117 PTHR10436 none none none
SUPERFAMILY 4 110 107 SSF55120 none none IPR020103

1 Localization

Analysis Start End Length
TMHMM 127 146 19

7 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_rEpiBC*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 35,77 14,11 55,31 lod 2,9413 6,2
Bourran2_2015_nEpiBC_3P Qrob_Chr12 12 s_1B73S5_217 v_7050_211 28,31 26,37 28,45 lod 4.5 11.6
Bourran2_2014_aSeqBC_A4 Qrob_Chr08 8 v_15999_278 v_AP13YL15_395 32,52 4,22 57,22 lod 2,7561 6,7
Bourran2_2014_nFork*_A4 Qrob_Chr08 8 PIE175 s_1CD7GJ_1398 31,22 5,24 57,24 lod 2,6724 6,8
Bourran2_2015_rEpiBC_3P Qrob_Chr08 8 s_A9TNV_543 v_11837_70 9,93 9,83 11,15 lod 3.3 7.3
Champenoux_2015_nSeqBC_A4 Qrob_Chr08 8 v_AD7YD13_501 s_1A7IED_780 43,44 43,42 43,99 lod 3.7 8.9
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL3_d13Cf Qrob_Chr08 8 v_5216_549 v_11625_20 37.08 12,26 54,9 lod 6.5888 0.04

0 Targeting