Protein : Qrob_P0430920.2 Q. robur
Protein Identifier  ? Qrob_P0430920.2 Organism . Name  Quercus robur
Score  0.0 Score Type  egn
Protein Description  (M=3) PF11960 - Domain of unknown function (DUF3474) Gene Prediction Quality  validated
Protein length 

Sequence

Length: 204  
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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0055114 oxidation-reduction process A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from each of two donors, and molecular oxygen is reduced to two molecules of water.

25 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|tcc:TCM_041603 1 172 + 172 Gaps:18 39.90 391 56.41 3e-45 Fatty acid desaturase 8 isoform 1
blastp_kegg lcl|mdm:103447381 6 172 + 167 Gaps:20 39.95 388 55.48 2e-43 omega-3 fatty acid desaturase endoplasmic reticulum-like
blastp_kegg lcl|pxb:103931220 6 172 + 167 Gaps:20 39.95 388 55.48 1e-42 omega-3 fatty acid desaturase endoplasmic reticulum-like
blastp_kegg lcl|pper:PRUPE_ppa007057mg 9 172 + 164 Gaps:21 39.84 384 54.90 3e-42 hypothetical protein
blastp_kegg lcl|pmum:103320972 9 172 + 164 Gaps:21 39.84 384 54.25 4e-42 omega-3 fatty acid desaturase endoplasmic reticulum-like
blastp_kegg lcl|pmum:103341784 6 171 + 166 Gaps:20 33.55 453 55.92 1e-41 omega-3 fatty acid desaturase chloroplastic-like
blastp_kegg lcl|pop:POPTR_0001s25980g 1 172 + 172 Gaps:16 40.73 383 55.77 5e-41 POPTRDRAFT_1067894 omega-3 desaturase family protein
blastp_kegg lcl|fve:101306021 18 179 + 162 Gaps:23 39.53 387 56.21 6e-41 omega-3 fatty acid desaturase chloroplastic-like
blastp_kegg lcl|pper:PRUPE_ppa005574mg 6 171 + 166 Gaps:20 33.55 453 55.26 9e-41 hypothetical protein
blastp_kegg lcl|gmx:100217335 17 171 + 155 Gaps:19 30.97 452 60.00 1e-40 FAD8-1 fatty acid desaturase 8
blastp_uniprot_sprot sp|P48624|FAD3E_BRANA 12 172 + 161 Gaps:16 37.86 383 57.24 2e-40 Omega-3 fatty acid desaturase endoplasmic reticulum OS Brassica napus GN FAD3 PE 2 SV 1
blastp_uniprot_sprot sp|P46310|FAD3C_ARATH 25 171 + 147 Gaps:18 29.37 446 58.78 4e-37 Omega-3 fatty acid desaturase chloroplastic OS Arabidopsis thaliana GN FAD7 PE 1 SV 1
blastp_uniprot_sprot sp|P48622|FAD3D_ARATH 24 171 + 148 Gaps:18 30.34 435 57.58 1e-36 Temperature-sensitive omega-3 fatty acid desaturase chloroplastic OS Arabidopsis thaliana GN FAD8 PE 2 SV 1
blastp_uniprot_sprot sp|P48619|FAD3C_RICCO 5 171 + 167 Gaps:20 33.26 460 53.59 1e-36 Omega-3 fatty acid desaturase chloroplastic OS Ricinus communis GN FAD7A-1 PE 2 SV 1
blastp_uniprot_sprot sp|P48618|FAD3C_BRANA 28 171 + 144 Gaps:18 31.68 404 57.81 6e-36 Omega-3 fatty acid desaturase chloroplastic (Fragment) OS Brassica napus GN FAD7 PE 2 SV 1
blastp_uniprot_sprot sp|P48623|FAD3E_ARATH 5 172 + 168 Gaps:16 39.38 386 51.97 7e-36 Omega-3 fatty acid desaturase endoplasmic reticulum OS Arabidopsis thaliana GN FAD3 PE 2 SV 1
blastp_uniprot_sprot sp|P48620|FAD3C_SESIN 19 171 + 153 Gaps:21 29.98 447 55.97 2e-35 Omega-3 fatty acid desaturase chloroplastic OS Sesamum indicum GN FAD7 PE 2 SV 1
blastp_uniprot_sprot sp|P48621|FAD3C_SOYBN 6 171 + 166 Gaps:18 33.55 453 54.61 5e-35 Omega-3 fatty acid desaturase chloroplastic OS Glycine max GN FAD7 PE 2 SV 1
blastp_uniprot_sprot sp|P32291|FAD3E_VIGRR 20 172 + 153 Gaps:18 36.58 380 54.68 5e-34 Omega-3 fatty acid desaturase endoplasmic reticulum OS Vigna radiata var. radiata GN ARG1 PE 2 SV 1
blastp_uniprot_sprot sp|P48626|FAD3E_TOBAC 18 172 + 155 Gaps:23 38.52 379 50.00 7e-34 Omega-3 fatty acid desaturase endoplasmic reticulum OS Nicotiana tabacum GN FAD3 PE 2 SV 1
rpsblast_cdd gnl|CDD|178114 14 171 + 158 Gaps:20 32.00 450 58.33 1e-40 PLN02498 PLN02498 omega-3 fatty acid desaturase.
rpsblast_cdd gnl|CDD|152395 4 74 + 71 Gaps:8 48.15 135 52.31 4e-13 pfam11960 DUF3474 Domain of unknown function (DUF3474). This presumed domain is functionally uncharacterized. This domain is found in bacteria and eukaryotes. This domain is typically between 126 to 140 amino acids in length. This domain is found associated with pfam00487.
rpsblast_cdd gnl|CDD|58173 52 172 + 121 Gaps:16 47.30 222 36.19 4e-11 cd03507 Delta12-FADS-like The Delta12 Fatty Acid Desaturase (Delta12-FADS)-like CD includes the integral-membrane enzymes delta-12 acyl-lipid desaturases oleate 12-hydroxylases omega3 and omega6 fatty acid desaturases and other related proteins found in a wide range of organisms including higher plants green algae diatoms nematodes fungi and bacteria. The expression of these proteins appears to be temperature dependent: decreases in temperature result in increased levels of fatty acid desaturation within membrane lipids subsequently altering cell membrane fluidity. An important enzyme for the production of polyunsaturates in plants is the oleate delta-12 desaturase (Arabidopsis FAD2) of the endoplasmic reticulum. This enzyme accepts l-acyl-2-oleoyl-sn-glycero-3-phosphocholine as substrate and requires NADH:cytochrome b oxidoreductase cytochrome b and oxygen for activity. FAD2 converts oleate(18:1) to linoleate (18:2) and is closely related to oleate 12-hydroxylase which catalyzes the hydroxylation of oleate to ricinoleate. Plastid-bound desaturases (Arabidopsis delta-12 desaturase (FAD6) omega-3 desaturase (FAD8) omega-6 desaturase (FAD6)) as well as the cyanobacterial thylakoid-bound FADSs require oxygen ferredoxin and ferredoxin oxidoreductase for activity. As in higher plants the cyanobacteria delta-12 (DesA) and omega-3 (DesB) FADSs desaturate oleate (18:1) to linoleate (18:2) and linoleate (18:2) to linolenate (18:3) respectively. Omega-3 (DesB/FAD8) and omega-6 (DesD/FAD6) desaturases catalyze reactions that introduce a double bond between carbons three and four and carbons six and seven respectively from the methyl end of fatty acids. As with other members of this superfamily this domain family has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain eight histidine residues: HXXXH HXX(X)HH and HXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the homologue stearoyl CoA desaturase. Mutation of any one of four of these histidines in the Synechocystis delta-12 acyl-lipid desaturase resulted in complete inactivity..
rpsblast_cdd gnl|CDD|178121 30 172 + 143 Gaps:25 35.17 381 31.34 1e-10 PLN02505 PLN02505 omega-6 fatty acid desaturase.
rpsblast_cdd gnl|CDD|33051 25 168 + 144 Gaps:16 37.32 343 24.22 2e-08 COG3239 DesA Fatty acid desaturase [Lipid metabolism].

10 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Phobius 149 203 55 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 91 96 6 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 97 125 29 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Pfam 14 74 61 PF11960 "UniPathway:UPA00658" Domain of unknown function (DUF3474) IPR021863
PANTHER 24 172 149 PTHR32100 none none none
PANTHER 24 172 149 PTHR32100:SF12 none none none
Phobius 131 148 18 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 1 66 66 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 67 90 24 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 126 130 5 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none

3 Localization

Analysis Start End Length
TMHMM 67 89 22
TMHMM 133 155 22
TMHMM 101 123 22

1 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Champenoux_2015_nPriLBD_3P Qrob_Chr10 10 v_15000_157 v_15000_310 15,68 15,9 15,91 lod 2.4 5.5

0 Targeting