Protein : Qrob_P0395650.2 Q. robur
Protein Identifier  ? Qrob_P0395650.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=25) 3.1.3.2 - Acid phosphatase. Code Enzyme  EC:3.1.3.2
Gene Prediction Quality  validated Protein length 

Sequence

Length: 249  
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0 Synonyms

1 GO Terms

Identifier Name Description
GO:0003993 acid phosphatase activity Catalysis of the reaction: an orthophosphoric monoester + H2O = an alcohol + phosphate, with an acid pH optimum.

26 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pper:PRUPE_ppa010268mg 26 248 + 223 Gaps:1 87.50 256 75.89 1e-121 hypothetical protein
blastp_kegg lcl|pmum:103340796 26 248 + 223 Gaps:1 87.50 256 75.45 1e-121 acid phosphatase 1
blastp_kegg lcl|mdm:103449864 25 248 + 224 none 87.16 257 73.66 1e-119 acid phosphatase 1-like
blastp_kegg lcl|pxb:103943925 25 248 + 224 none 86.82 258 73.66 4e-119 acid phosphatase 1
blastp_kegg lcl|mdm:103431530 25 248 + 224 none 86.49 259 73.66 6e-119 acid phosphatase 1-like
blastp_kegg lcl|cic:CICLE_v10009222mg 26 248 + 223 Gaps:1 85.50 262 72.77 3e-118 hypothetical protein
blastp_kegg lcl|cit:102629285 26 248 + 223 Gaps:1 85.50 262 72.77 3e-118 acid phosphatase 1-like
blastp_kegg lcl|tcc:TCM_037750 26 248 + 223 none 69.04 323 74.89 5e-118 HAD superfamily isoform 1
blastp_kegg lcl|pop:POPTR_0006s15760g 15 247 + 233 none 94.33 247 68.67 4e-117 POPTRDRAFT_416559 hypothetical protein
blastp_kegg lcl|fve:101302001 7 248 + 242 none 95.65 253 67.77 2e-116 acid phosphatase 1-like
blastp_pdb 2i34_B 100 245 + 146 Gaps:37 60.85 258 34.39 1e-11 mol:protein length:258 acid phosphatase
blastp_pdb 2i34_A 100 245 + 146 Gaps:37 60.85 258 34.39 1e-11 mol:protein length:258 acid phosphatase
blastp_pdb 2i33_B 100 245 + 146 Gaps:37 60.85 258 34.39 1e-11 mol:protein length:258 Acid phosphatase
blastp_pdb 2i33_A 100 245 + 146 Gaps:37 60.85 258 34.39 1e-11 mol:protein length:258 Acid phosphatase
blastp_uniprot_sprot sp|P27061|PPA1_SOLLC 21 247 + 227 none 89.02 255 52.86 2e-85 Acid phosphatase 1 OS Solanum lycopersicum GN APS1 PE 2 SV 1
blastp_uniprot_sprot sp|P15490|VSPA_SOYBN 33 247 + 215 Gaps:4 84.65 254 46.05 6e-60 Stem 28 kDa glycoprotein OS Glycine max GN VSPA PE 2 SV 1
blastp_uniprot_sprot sp|P10743|VSPB_SOYBN 33 247 + 215 Gaps:3 84.25 254 46.73 8e-59 Stem 31 kDa glycoprotein OS Glycine max GN VSPB PE 2 SV 1
blastp_uniprot_sprot sp|P10742|S25K_SOYBN 33 243 + 211 Gaps:4 72.51 291 45.50 5e-57 Stem 31 kDa glycoprotein (Fragment) OS Glycine max GN VSP25 PE 2 SV 2
blastp_uniprot_sprot sp|O82122|VSP2_ARATH 36 247 + 212 Gaps:4 80.00 265 41.04 3e-50 Vegetative storage protein 2 OS Arabidopsis thaliana GN VSP2 PE 1 SV 1
blastp_uniprot_sprot sp|O49195|VSP1_ARATH 36 247 + 212 Gaps:4 78.52 270 40.09 4e-49 Vegetative storage protein 1 OS Arabidopsis thaliana GN VSP1 PE 1 SV 2
blastp_uniprot_sprot sp|O04195|Y2992_ARATH 3 245 + 243 Gaps:30 78.09 283 25.79 6e-08 Uncharacterized protein At2g39920 OS Arabidopsis thaliana GN At2g39920 PE 2 SV 2
rpsblast_cdd gnl|CDD|130736 28 247 + 220 none 96.07 229 50.91 9e-77 TIGR01675 plant-AP plant acid phosphatase. This model represents a family of acid phosphatase from plants which are most closely related to the (so called) class B non-specific acid phosphatase OlpA (TIGR01533 which is believed to be a 5'-nucleotide phosphatase) and somewhat more distantly to another class B phosphatase AphA (TIGR01672). Together these three clades define a subfamily (pfam03767) which corresponds to the IIIB subfamily of the haloacid dehalogenase (HAD) superfamily of aspartate nucleophile hydrolases. It has been reported that the best substrate for this enzyme that could be found was purine 5'-nucleoside phosphates. This is in concordance with the assignment of the H. influenzae hel protein (from TIGR01533) as a 5'-nucleotidase however there is presently no other evidence to support this specific function for these plant phosphatases. Many genes from this family have been annotated as vegetative storage proteins due to their close homology with these earlier-characterized gene products which are highly expressed in leaves. There are significant differences however including expression levels and distribution. The most important difference is the lack in authentic VSPs of the nucleophilic aspartate residue which is instead replaced by serine glycine or asparagine. Thus these proteins can not be expected to be active phosphatases. This issue was confused by the publication in 1992 of an article claiming activity for the Glycine max VSP. In 1994 this assertion was refuted by the separation of the activity from the VSP. This model explicitly excludes the VSPs which lack the nucleophilc aspartate. The possibility exists however that some members of this family may while containing all of the conserved HAD-superfamily catalytic residues lack activity and have a function related to the function of the VSPs rather than the acid phosphatases.
rpsblast_cdd gnl|CDD|202762 37 247 + 211 Gaps:1 99.53 213 54.72 2e-75 pfam03767 Acid_phosphat_B HAD superfamily subfamily IIIB (Acid phosphatase). This family proteins includes acid phosphatases and a number of vegetative storage proteins.
rpsblast_cdd gnl|CDD|130741 31 248 + 218 Gaps:5 79.64 275 46.12 2e-51 TIGR01680 Veg_Stor_Prot vegetative storage protein. The proteins represented by this model are close relatives of the plant acid phosphatases (TIGR01675) are limited to members of the Phaseoleae including Glycine max (soybean) and Phaseolus vulgaris (kidney bean). These proteins are highly expressed in the leaves of repeatedly depodded plants. VSP differs most strinkingly from the acid phosphatases in the lack of the conserved nucleophilic aspartate residue in the N-terminus thus they should be inactive as phosphatases. This issue was confused by the publication in 1992 of an article claiming activity for the Glycine max VSP. In 1994 this assertion was refuted by the separation of the activity from the VSP.
rpsblast_cdd gnl|CDD|162404 100 245 + 146 Gaps:31 59.77 266 32.08 2e-13 TIGR01533 lipo_e_P4 5'-nucleotidase lipoprotein e(P4) family. This model represents a set of bacterial lipoproteins belonging to a larger acid phosphatase family (pfam03767) which in turn belongs to the haloacid dehalogenase (HAD) superfamily of aspartate-dependent hydrolases. Members are found on the outer membrane of Gram-negative bacteria and the cytoplasmic membrane of Gram-positive bacteria. Most members have classic lipoprotein signal sequences. A critical role of this 5'-nucleotidase in Haemophilus influenzae is the degradation of external riboside in order to allow transport into the cell. An earlier suggested role in hemin transport is no longer current. This enzyme may also have other physiologically significant roles.
rpsblast_cdd gnl|CDD|32577 100 245 + 146 Gaps:35 58.03 274 25.79 3e-08 COG2503 COG2503 Predicted secreted acid phosphatase [General function prediction only].

12 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Pfam 30 247 218 PF03767 none HAD superfamily, subfamily IIIB (Acid phosphatase) IPR005519
PANTHER 26 247 222 PTHR31284:SF6 none none none
Phobius 20 248 229 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
PIRSF 10 248 239 PIRSF002674 none none IPR014403
Phobius 4 15 12 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
Phobius 1 19 19 SIGNAL_PEPTIDE none Signal peptide region none
SUPERFAMILY 97 246 150 SSF56784 none none IPR023214
Phobius 16 19 4 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
Gene3D 99 245 147 G3DSA:3.40.50.1000 none none IPR023214
PANTHER 26 247 222 PTHR31284 none none none
TIGRFAM 27 247 221 TIGR01675 none plant-AP: plant acid phosphatase IPR010028
Phobius 1 3 3 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none

1 Localization

Analysis Start End Length
SignalP_EUK 1 19 18

13 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran1_2003_QTL2_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 87 lod 3,3 8,7
Bourran2_2004_QTL9_peak_Bud_burst_3P Qrob_Chr02 2 s_1C34E9_788 v_12238_322 50 25 75 lod 4,4 10,1
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL2_d13Cf Qrob_Chr02 2 s_1AQA4Z_1644 s_1AK5QX_947 53.67 14,01 79,68 lod 5.6594 0.03
Bourran1_2004_QTL2_peak_Bud_burst_3P Qrob_Chr02 2 s_1AW12F_382 s_1A77MR_223 42 6 64 lod 3,6 9,6
Bourran_2000_2002_QTL2_Delta.F Qrob_Chr02 2 s_1CSO13_1244 s_1AVEUF_1540 55.44 46,71 63,68 lod 7.3232 0.058
Bourran2_2002_QTL7_peak_Bud_burst_3P Qrob_Chr02 2 s_1ANG6_1446 v_11270_161 40 29 52 lod 8,1 16
Bourran2_2002_QTL9_peak_Bud_burst_A4 Qrob_Chr02 2 s_1BFNDA_375 s_1A3VA1_2139 32,5 17 62 lod 3,1 4,2
Bourran2_2003_QTL8_peak_Bud_burst_3P Qrob_Chr02 2 s_1ANG6_1446 v_11270_161 40 0 72 lod 4,4 9,9
Bourran2_2014_nP_A4 Qrob_Chr11 11 s_1B58GB_1413 s_1A5BYY_1671 11,15 0 42,38 lod 1,8913 4,5
Bourran2_2015_nP_A4 Qrob_Chr02 2 s_1A0FUE_1868 s_1A1UAI_500 20,64 20,47 21,36 lod 5.8 10.9
Bourran2_2015_nPriLBD_A4 Qrob_Chr02 2 s_1CP5DI_1183 s_1A63ZX_1277 24,87 24,63 26,18 lod 3.8 7
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL6_d13Cf Qrob_Chr02 2 s_1AEP21_172 v_6048_204 46.33 22,5 65,23 lod 4.972 0.03
Bourran1_2004_QTL3_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 46 lod 2,9 6,4

0 Targeting