Protein : Qrob_P0377170.2 Q. robur
Protein Identifier  ? Qrob_P0377170.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=1) K02684 - DNA primase small subunit [EC:2.7.7.-] Gene Prediction Quality  validated
Protein length 

Sequence

Length: 459  
Kegg Orthology  K02684
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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0003896 DNA primase activity Catalysis of the synthesis of a short RNA primer on a DNA template, providing a free 3'-OH that can be extended by DNA-directed DNA polymerases. Catalyzed by a DNA-directed RNA polymerase that forms a complex with alpha DNA polymerase.
GO:0006269 DNA replication, synthesis of RNA primer The synthesis of a short RNA polymer, usually 4-15 nucleotides long, using one strand of unwound DNA as a template; the RNA then serves as a primer from which DNA polymerases extend synthesis.

27 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|vvi:100260326 4 458 + 455 Gaps:1 99.78 457 79.39 0.0 DNA primase small subunit-like
blastp_kegg lcl|tcc:TCM_016451 1 458 + 458 Gaps:8 100.00 452 79.87 0.0 DNA primase isoform 1
blastp_kegg lcl|pmum:103337654 1 458 + 458 Gaps:8 100.00 456 78.51 0.0 DNA primase small subunit
blastp_kegg lcl|pper:PRUPE_ppa005529mg 1 458 + 458 Gaps:8 100.00 456 78.73 0.0 hypothetical protein
blastp_kegg lcl|cit:102613266 1 458 + 458 Gaps:9 100.00 451 78.27 0.0 DNA primase small subunit-like
blastp_kegg lcl|pop:POPTR_0003s10380g 1 458 + 458 Gaps:10 100.00 450 77.78 0.0 POPTRDRAFT_711657 hypothetical protein
blastp_kegg lcl|sot:102590488 1 458 + 458 Gaps:6 100.00 454 75.33 0.0 DNA primase small subunit-like
blastp_kegg lcl|sly:101258292 1 458 + 458 Gaps:6 100.00 454 74.89 0.0 DNA primase small subunit-like
blastp_kegg lcl|mus:103996854 1 458 + 458 Gaps:4 100.00 456 72.81 0.0 DNA primase small subunit
blastp_kegg lcl|pda:103719443 1 458 + 458 Gaps:4 100.00 456 72.81 0.0 DNA primase small subunit
blastp_uniprot_sprot sp|P20664|PRI1_MOUSE 40 443 + 404 Gaps:22 92.57 417 47.15 1e-117 DNA primase small subunit OS Mus musculus GN Prim1 PE 1 SV 1
blastp_uniprot_sprot sp|P49642|PRI1_HUMAN 40 443 + 404 Gaps:23 92.62 420 47.30 3e-117 DNA primase small subunit OS Homo sapiens GN PRIM1 PE 1 SV 1
blastp_uniprot_sprot sp|Q24317|PRI1_DROME 38 401 + 364 Gaps:21 81.51 438 45.66 6e-103 DNA primase small subunit OS Drosophila melanogaster GN DNApol-alpha50 PE 2 SV 2
blastp_uniprot_sprot sp|O14215|PRI1_SCHPO 43 401 + 359 Gaps:27 76.65 454 44.54 9e-96 DNA primase small subunit OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN pri1 PE 1 SV 1
blastp_uniprot_sprot sp|Q25998|PRI1_PLAFK 36 440 + 405 Gaps:37 93.36 452 40.05 1e-92 DNA primase small subunit OS Plasmodium falciparum (isolate K1 / Thailand) PE 3 SV 1
blastp_uniprot_sprot sp|Q7KQM1|PRI1_PLAF7 36 440 + 405 Gaps:37 93.36 452 40.05 1e-92 DNA primase small subunit OS Plasmodium falciparum (isolate 3D7) GN PF14_0366 PE 3 SV 1
blastp_uniprot_sprot sp|P10363|PRI1_YEAST 33 443 + 411 Gaps:38 96.09 409 39.95 2e-78 DNA primase small subunit OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN PRI1 PE 1 SV 2
blastp_uniprot_sprot sp|P34471|PRI1_CAEEL 40 412 + 373 Gaps:48 90.00 410 38.48 1e-69 DNA primase small subunit OS Caenorhabditis elegans GN pri-1 PE 3 SV 1
blastp_uniprot_sprot sp|O26685|PRIS_METTH 89 391 + 303 Gaps:45 82.35 323 30.45 5e-13 DNA primase small subunit PriS OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) GN priS PE 3 SV 1
blastp_uniprot_sprot sp|A5UJA2|PRIS_METS3 75 399 + 325 Gaps:52 89.20 324 30.10 3e-10 DNA primase small subunit PriS OS Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) GN priS PE 3 SV 1
rpsblast_cdd gnl|CDD|80310 49 387 + 339 Gaps:15 100.00 232 45.69 1e-58 cd04860 AE_Prim_S AE_Prim_S: primase domain similar to that found in the small subunit of archaeal and eukaryotic (A/E) DNA primases. Primases are DNA-dependent RNA polymerases which synthesis the short RNA primers required for DNA replication. In addition to its catalytic role in replication DNA primase may play a role in coupling replication to DNA damage repair and in checkpoint control during S phase. In eukaryotes this small catalytically active primase subunit (p50) and a larger primase subunit (p60) referred to jointly as the core primase associate with the B subunit and the DNA polymerase alpha subunit in a complex called Pol alpha-pri. The function of the larger primase subunit is unclear. Included in this group are Pfu41 and Pfu46 these two proteins comprise the primase complex of the archaea Pyrococcus furiosus Pfu41 and Pfu46 have sequence identity to the eukaryotic p50 and p60 primase proteins respectively. Pfu41 preferentially uses dNTPs as substrate. Pfu46 regulates the primase activity of Pfu41..
rpsblast_cdd gnl|CDD|31656 38 398 + 361 Gaps:39 94.43 341 25.47 2e-43 COG1467 PRI1 Eukaryotic-type DNA primase catalytic (small) subunit [DNA replication recombination and repair].
rpsblast_cdd gnl|CDD|161824 41 387 + 347 Gaps:68 94.61 297 25.98 9e-31 TIGR00335 primase_sml DNA primase eukaryotic-type small subunit putative. Archaeal members differ substantially from eukaryotic members and should be considered putative pending experimental evidence. The protein is universal and single copy among completed archaeal and eukarotic genomes to date. DNA primase creates RNA primers needed for DNA replication.This model is named putative because the assignment is putative for archaeal proteins. Eukaryotic proteins scoring above the trusted cutoff can be considered authentic.
rpsblast_cdd gnl|CDD|202033 144 379 + 236 Gaps:6 100.00 133 48.87 4e-24 pfam01896 DNA_primase_S Eukaryotic and archaeal DNA primase small subunit. DNA primase synthesises the RNA primers for the Okazaki fragments in lagging strand DNA synthesis. DNA primase is a heterodimer of large and small subunits. This family also includes baculovirus late expression factor 1 or LEF-1 proteins. Baculovirus LEF-1 is a DNA primase enzyme. Bacterial DNA primase adopts a different fold to archaeal and eukaryotic primases.
rpsblast_cdd gnl|CDD|179016 75 392 + 318 Gaps:60 78.72 376 25.34 5e-18 PRK00419 PRK00419 DNA primase small subunit Reviewed.
rpsblast_cdd gnl|CDD|80308 76 207 + 132 Gaps:30 77.94 136 24.53 1e-09 cd00525 AE_Prim_S_like AE_Prim_S_like: primase domain similar to that found in the small subunit of archaeal and eukaryotic (A/E) DNA primases. The replication machineries of A/Es are distinct from that of bacteria. Primases are DNA-dependent RNA polymerases which synthesis the short RNA primers required for DNA replication. In eukaryotes this small catalytically active primase subunit (p50) and a larger primase subunit (p60) referred to jointly as the core primase associate with the B subunit and the DNA polymerase alpha subunit in a complex called Pol alpha-pri. In addition to its catalytic role in replication eukaryotic DNA primase may play a role in coupling replication to DNA damage repair and in checkpoint control during S phase. Pfu41 and Pfu46 comprise the primase complex of the archaea Pyrococcus furiosus these proteins have sequence identity to the eukaryotic p50 and p60 primase proteins respectively. Pfu41 preferentially uses dNTPs as substrate. Pfu46 regulates the primase activity of Pfu41. Also found in this group is the primase-polymerase (primpol) domain of replicases from archaeal plasmids including the ORF904 protein of pRN1 from Sulfolobus islandicus (pRN1 primpol). The pRN1 primpol domain exhibits DNA polymerase and primase activities a cluster of active site residues (three acidic residues and a histidine) is required for both these activities. The pRN1 primpol primase activity prefers dNTPs to rNTPs however incorporation of dNTPs requires rNTP as cofactor. This group also includes the Pol domain of bacterial LigD proteins such Mycobacterium tuberculosis (Mt)LigD. MtLigD contains an N-terminal Pol domain a central phosphoesterase module and a C-terminal ligase domain. LigD Pol plays a role in non-homologous end joining (NHEJ)-mediated repair of DNA double-strand breaks (DSB) in vivo perhaps by filling in short 5'-overhangs with ribonucleotides the filled in termini would be sealed by the associated LigD ligase domain. The MtLigD Pol domain is stimulated by manganese is error-prone and prefers adding rNTPs to dNTPs in vitro..
rpsblast_kog gnl|CDD|38062 40 442 + 403 Gaps:26 95.87 412 50.38 1e-132 KOG2851 KOG2851 KOG2851 Eukaryotic-type DNA primase catalytic (small) subunit [Replication recombination and repair].

6 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
SUPERFAMILY 37 400 364 SSF56747 none none none
Pfam 144 379 236 PF01896 none Eukaryotic and archaeal DNA primase small subunit IPR002755
PANTHER 4 455 452 PTHR10536 none none IPR014052
Gene3D 36 226 191 G3DSA:3.90.920.10 none none none
Gene3D 348 401 54 G3DSA:3.90.920.10 none none none
TIGRFAM 41 398 358 TIGR00335 none primase_sml: putative DNA primase, eukaryotic-type, small subunit IPR014052

0 Localization

17 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran1_2003_QTL2_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 87 lod 3,3 8,7
Bourran2_2004_QTL9_peak_Bud_burst_3P Qrob_Chr02 2 s_1C34E9_788 v_12238_322 50 25 75 lod 4,4 10,1
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL2_d13Cf Qrob_Chr02 2 s_1AQA4Z_1644 s_1AK5QX_947 53.67 14,01 79,68 lod 5.6594 0.03
Bourran1_2004_QTL2_peak_Bud_burst_3P Qrob_Chr02 2 s_1AW12F_382 s_1A77MR_223 42 6 64 lod 3,6 9,6
Bourran2_2002_QTL7_peak_Bud_burst_3P Qrob_Chr02 2 s_1ANG6_1446 v_11270_161 40 29 52 lod 8,1 16
Bourran2_2002_QTL9_peak_Bud_burst_A4 Qrob_Chr02 2 s_1BFNDA_375 s_1A3VA1_2139 32,5 17 62 lod 3,1 4,2
Bourran2_2003_QTL8_peak_Bud_burst_3P Qrob_Chr02 2 s_1ANG6_1446 v_11270_161 40 0 72 lod 4,4 9,9
Bourran2_2014_nP_A4 Qrob_Chr11 11 s_1B58GB_1413 s_1A5BYY_1671 11,15 0 42,38 lod 1,8913 4,5
Bourran2_2015_nP_A4 Qrob_Chr02 2 s_1A0FUE_1868 s_1A1UAI_500 20,64 20,47 21,36 lod 5.8 10.9
Bourran2_2015_nPriLBD_A4 Qrob_Chr02 2 s_1CP5DI_1183 s_1A63ZX_1277 24,87 24,63 26,18 lod 3.8 7
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL6_d13Cf Qrob_Chr02 2 s_1AEP21_172 v_6048_204 46.33 22,5 65,23 lod 4.972 0.03
Bourran2_2015_nEpis_A4 Qrob_Chr09 9 v_15847_485 v_8329_369 34,94 34,88 37,45 lod 3.1 7
Bourran2_2015_nSecLBD_A4 Qrob_Chr09 9 v_15847_485 v_8329_369 35,81 34,88 37,45 lod 4.4 10.4
Bourran1_2004_QTL3_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 46 lod 2,9 6,4
Bourran2_2015_nEpiBC_A4 Qrob_Chr07 7 s_1DP9TW_798 v_8128_173 22,61 22,14 22,73 lod 3.1 8.5
Champenoux_2015_nP_A4 Qrob_Chr02 2 s_1BN4CB_644 v_508_128 23,76 23,06 24,51 lod 2.8 6.2
Champenoux_2015_nPriLBD_A4 Qrob_Chr02 2 s_1CP5DI_1183 s_1A63ZX_1277 25,35 24,63 26,18 lod 4.0 8.7

0 Targeting